The DrosDel Collection

We describe a collection of P-element insertions that have considerable utility for generating custom chromosomal aberrations in Drosophila melanogaster. We have mobilized a pair of engineered P elements, p{RS3} and p{RS5}, to collect 3243 lines unambiguously mapped to the Drosophila genome sequence...

Ausführliche Beschreibung

Gespeichert in:
Bibliographische Detailangaben
Veröffentlicht in:Genetics (Austin) 2004-06, Vol.167 (2), p.797-813
Hauptverfasser: Ryder, Edward, Blows, Fiona, Ashburner, Michael, Bautista-Llacer, Rosa, Coulson, Darin, Drummond, Jenny, Webster, Jane, Gubb, David, Gunton, Nicola, Johnson, Glynnis, O'Kane, Cahir J, Huen, David, Sharma, Punita, Asztalos, Zoltan, Baisch, Heiko, Schulze, Janet, Kube, Maria, Kittlaus, Kathrin, Reuter, Gunter, Maroy, Peter, Szidonya, Janos, Rasmuson-Lestander, Åsa, Ekström, Karin, Dickson, Barry, Hugentobler, Christoph, Stocker, Hugo, Hafen, Ernst, Lepesant, Jean Antoine, Pflugfelder, Gert, Heisenberg, Martin, Mechler, Bernard, Serras, Florenci, Corominas, Montserrat, Schneuwly, Stephan, Preat, Thomas, Roote, John, Russell, Steven
Format: Artikel
Sprache:eng
Online-Zugang:Volltext
Tags: Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
container_end_page 813
container_issue 2
container_start_page 797
container_title Genetics (Austin)
container_volume 167
creator Ryder, Edward
Blows, Fiona
Ashburner, Michael
Bautista-Llacer, Rosa
Coulson, Darin
Drummond, Jenny
Webster, Jane
Gubb, David
Gunton, Nicola
Johnson, Glynnis
O'Kane, Cahir J
Huen, David
Sharma, Punita
Asztalos, Zoltan
Baisch, Heiko
Schulze, Janet
Kube, Maria
Kittlaus, Kathrin
Reuter, Gunter
Maroy, Peter
Szidonya, Janos
Rasmuson-Lestander, Åsa
Ekström, Karin
Dickson, Barry
Hugentobler, Christoph
Stocker, Hugo
Hafen, Ernst
Lepesant, Jean Antoine
Pflugfelder, Gert
Heisenberg, Martin
Mechler, Bernard
Serras, Florenci
Corominas, Montserrat
Schneuwly, Stephan
Preat, Thomas
Roote, John
Russell, Steven
description We describe a collection of P-element insertions that have considerable utility for generating custom chromosomal aberrations in Drosophila melanogaster. We have mobilized a pair of engineered P elements, p{RS3} and p{RS5}, to collect 3243 lines unambiguously mapped to the Drosophila genome sequence. The collection contains, on average, an element every 35 kb. We demonstrate the utility of the collection for generating custom chromosomal deletions that have their end points mapped, with base-pair resolution, to the genome sequence. The collection was generated in an isogenic strain, thus affording a uniform background for screens where sensitivity to genetic background is high. The entire collection, along with a computational and genetic toolbox for designing and generating custom deletions, is publicly available. Using the collection it is theoretically possible to generate >12,000 deletions between 1 bp and 1 Mb in size by simple eye color selection. In addition, a further 37,000 deletions, selectable by molecular screening, may be generated. We are now using the collection to generate a second-generation deficiency kit that is precisely mapped to the genome sequence.
doi_str_mv 10.1534/genetics.104.026658
format Article
fullrecord <record><control><sourceid>crossref</sourceid><recordid>TN_cdi_crossref_primary_10_1534_genetics_104_026658</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>10_1534_genetics_104_026658</sourcerecordid><originalsourceid>FETCH-LOGICAL-c2058-234b04e6c986f18992b4ed6ad75dd598f731bc59e392c7faf1012d70c7b25b873</originalsourceid><addsrcrecordid>eNpNjz1PwzAURS1EJUrblYWlfyDhPX97RCkUpEos7WzF9nMbFBoUd-HfU1SQmO7VHY7uYeweoUYl5MOejnTqYqkRZA1ca2Wv2BSdFBXXAq__9Rt2W8o7AGin7JTdbQ-0XI1DWVG_bIa-p3jqhuOcTXLbF1r85oztnp-2zUu1eVu_No-bKnJQtuJCBpCko7M6o3WOB0lJt8molJSz2QgMUTkSjkeT24yAPBmIJnAVrBEzJi7ceL5QRsr-c-w-2vHLI_gfNf-ndh6kv6iJbyLhQis</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype></control><display><type>article</type><title>The DrosDel Collection</title><source>Oxford Journals【Remote access available】</source><source>Free E-Journal (出版社公開部分のみ)</source><source>Alma/SFX Local Collection</source><creator>Ryder, Edward ; Blows, Fiona ; Ashburner, Michael ; Bautista-Llacer, Rosa ; Coulson, Darin ; Drummond, Jenny ; Webster, Jane ; Gubb, David ; Gunton, Nicola ; Johnson, Glynnis ; O'Kane, Cahir J ; Huen, David ; Sharma, Punita ; Asztalos, Zoltan ; Baisch, Heiko ; Schulze, Janet ; Kube, Maria ; Kittlaus, Kathrin ; Reuter, Gunter ; Maroy, Peter ; Szidonya, Janos ; Rasmuson-Lestander, Åsa ; Ekström, Karin ; Dickson, Barry ; Hugentobler, Christoph ; Stocker, Hugo ; Hafen, Ernst ; Lepesant, Jean Antoine ; Pflugfelder, Gert ; Heisenberg, Martin ; Mechler, Bernard ; Serras, Florenci ; Corominas, Montserrat ; Schneuwly, Stephan ; Preat, Thomas ; Roote, John ; Russell, Steven</creator><creatorcontrib>Ryder, Edward ; Blows, Fiona ; Ashburner, Michael ; Bautista-Llacer, Rosa ; Coulson, Darin ; Drummond, Jenny ; Webster, Jane ; Gubb, David ; Gunton, Nicola ; Johnson, Glynnis ; O'Kane, Cahir J ; Huen, David ; Sharma, Punita ; Asztalos, Zoltan ; Baisch, Heiko ; Schulze, Janet ; Kube, Maria ; Kittlaus, Kathrin ; Reuter, Gunter ; Maroy, Peter ; Szidonya, Janos ; Rasmuson-Lestander, Åsa ; Ekström, Karin ; Dickson, Barry ; Hugentobler, Christoph ; Stocker, Hugo ; Hafen, Ernst ; Lepesant, Jean Antoine ; Pflugfelder, Gert ; Heisenberg, Martin ; Mechler, Bernard ; Serras, Florenci ; Corominas, Montserrat ; Schneuwly, Stephan ; Preat, Thomas ; Roote, John ; Russell, Steven</creatorcontrib><description>We describe a collection of P-element insertions that have considerable utility for generating custom chromosomal aberrations in Drosophila melanogaster. We have mobilized a pair of engineered P elements, p{RS3} and p{RS5}, to collect 3243 lines unambiguously mapped to the Drosophila genome sequence. The collection contains, on average, an element every 35 kb. We demonstrate the utility of the collection for generating custom chromosomal deletions that have their end points mapped, with base-pair resolution, to the genome sequence. The collection was generated in an isogenic strain, thus affording a uniform background for screens where sensitivity to genetic background is high. The entire collection, along with a computational and genetic toolbox for designing and generating custom deletions, is publicly available. Using the collection it is theoretically possible to generate &gt;12,000 deletions between 1 bp and 1 Mb in size by simple eye color selection. In addition, a further 37,000 deletions, selectable by molecular screening, may be generated. We are now using the collection to generate a second-generation deficiency kit that is precisely mapped to the genome sequence.</description><identifier>ISSN: 1943-2631</identifier><identifier>EISSN: 1943-2631</identifier><identifier>DOI: 10.1534/genetics.104.026658</identifier><language>eng</language><ispartof>Genetics (Austin), 2004-06, Vol.167 (2), p.797-813</ispartof><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c2058-234b04e6c986f18992b4ed6ad75dd598f731bc59e392c7faf1012d70c7b25b873</citedby><cites>FETCH-LOGICAL-c2058-234b04e6c986f18992b4ed6ad75dd598f731bc59e392c7faf1012d70c7b25b873</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,27924,27925</link.rule.ids></links><search><creatorcontrib>Ryder, Edward</creatorcontrib><creatorcontrib>Blows, Fiona</creatorcontrib><creatorcontrib>Ashburner, Michael</creatorcontrib><creatorcontrib>Bautista-Llacer, Rosa</creatorcontrib><creatorcontrib>Coulson, Darin</creatorcontrib><creatorcontrib>Drummond, Jenny</creatorcontrib><creatorcontrib>Webster, Jane</creatorcontrib><creatorcontrib>Gubb, David</creatorcontrib><creatorcontrib>Gunton, Nicola</creatorcontrib><creatorcontrib>Johnson, Glynnis</creatorcontrib><creatorcontrib>O'Kane, Cahir J</creatorcontrib><creatorcontrib>Huen, David</creatorcontrib><creatorcontrib>Sharma, Punita</creatorcontrib><creatorcontrib>Asztalos, Zoltan</creatorcontrib><creatorcontrib>Baisch, Heiko</creatorcontrib><creatorcontrib>Schulze, Janet</creatorcontrib><creatorcontrib>Kube, Maria</creatorcontrib><creatorcontrib>Kittlaus, Kathrin</creatorcontrib><creatorcontrib>Reuter, Gunter</creatorcontrib><creatorcontrib>Maroy, Peter</creatorcontrib><creatorcontrib>Szidonya, Janos</creatorcontrib><creatorcontrib>Rasmuson-Lestander, Åsa</creatorcontrib><creatorcontrib>Ekström, Karin</creatorcontrib><creatorcontrib>Dickson, Barry</creatorcontrib><creatorcontrib>Hugentobler, Christoph</creatorcontrib><creatorcontrib>Stocker, Hugo</creatorcontrib><creatorcontrib>Hafen, Ernst</creatorcontrib><creatorcontrib>Lepesant, Jean Antoine</creatorcontrib><creatorcontrib>Pflugfelder, Gert</creatorcontrib><creatorcontrib>Heisenberg, Martin</creatorcontrib><creatorcontrib>Mechler, Bernard</creatorcontrib><creatorcontrib>Serras, Florenci</creatorcontrib><creatorcontrib>Corominas, Montserrat</creatorcontrib><creatorcontrib>Schneuwly, Stephan</creatorcontrib><creatorcontrib>Preat, Thomas</creatorcontrib><creatorcontrib>Roote, John</creatorcontrib><creatorcontrib>Russell, Steven</creatorcontrib><title>The DrosDel Collection</title><title>Genetics (Austin)</title><description>We describe a collection of P-element insertions that have considerable utility for generating custom chromosomal aberrations in Drosophila melanogaster. We have mobilized a pair of engineered P elements, p{RS3} and p{RS5}, to collect 3243 lines unambiguously mapped to the Drosophila genome sequence. The collection contains, on average, an element every 35 kb. We demonstrate the utility of the collection for generating custom chromosomal deletions that have their end points mapped, with base-pair resolution, to the genome sequence. The collection was generated in an isogenic strain, thus affording a uniform background for screens where sensitivity to genetic background is high. The entire collection, along with a computational and genetic toolbox for designing and generating custom deletions, is publicly available. Using the collection it is theoretically possible to generate &gt;12,000 deletions between 1 bp and 1 Mb in size by simple eye color selection. In addition, a further 37,000 deletions, selectable by molecular screening, may be generated. We are now using the collection to generate a second-generation deficiency kit that is precisely mapped to the genome sequence.</description><issn>1943-2631</issn><issn>1943-2631</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2004</creationdate><recordtype>article</recordtype><recordid>eNpNjz1PwzAURS1EJUrblYWlfyDhPX97RCkUpEos7WzF9nMbFBoUd-HfU1SQmO7VHY7uYeweoUYl5MOejnTqYqkRZA1ca2Wv2BSdFBXXAq__9Rt2W8o7AGin7JTdbQ-0XI1DWVG_bIa-p3jqhuOcTXLbF1r85oztnp-2zUu1eVu_No-bKnJQtuJCBpCko7M6o3WOB0lJt8molJSz2QgMUTkSjkeT24yAPBmIJnAVrBEzJi7ceL5QRsr-c-w-2vHLI_gfNf-ndh6kv6iJbyLhQis</recordid><startdate>20040601</startdate><enddate>20040601</enddate><creator>Ryder, Edward</creator><creator>Blows, Fiona</creator><creator>Ashburner, Michael</creator><creator>Bautista-Llacer, Rosa</creator><creator>Coulson, Darin</creator><creator>Drummond, Jenny</creator><creator>Webster, Jane</creator><creator>Gubb, David</creator><creator>Gunton, Nicola</creator><creator>Johnson, Glynnis</creator><creator>O'Kane, Cahir J</creator><creator>Huen, David</creator><creator>Sharma, Punita</creator><creator>Asztalos, Zoltan</creator><creator>Baisch, Heiko</creator><creator>Schulze, Janet</creator><creator>Kube, Maria</creator><creator>Kittlaus, Kathrin</creator><creator>Reuter, Gunter</creator><creator>Maroy, Peter</creator><creator>Szidonya, Janos</creator><creator>Rasmuson-Lestander, Åsa</creator><creator>Ekström, Karin</creator><creator>Dickson, Barry</creator><creator>Hugentobler, Christoph</creator><creator>Stocker, Hugo</creator><creator>Hafen, Ernst</creator><creator>Lepesant, Jean Antoine</creator><creator>Pflugfelder, Gert</creator><creator>Heisenberg, Martin</creator><creator>Mechler, Bernard</creator><creator>Serras, Florenci</creator><creator>Corominas, Montserrat</creator><creator>Schneuwly, Stephan</creator><creator>Preat, Thomas</creator><creator>Roote, John</creator><creator>Russell, Steven</creator><scope>AAYXX</scope><scope>CITATION</scope></search><sort><creationdate>20040601</creationdate><title>The DrosDel Collection</title><author>Ryder, Edward ; Blows, Fiona ; Ashburner, Michael ; Bautista-Llacer, Rosa ; Coulson, Darin ; Drummond, Jenny ; Webster, Jane ; Gubb, David ; Gunton, Nicola ; Johnson, Glynnis ; O'Kane, Cahir J ; Huen, David ; Sharma, Punita ; Asztalos, Zoltan ; Baisch, Heiko ; Schulze, Janet ; Kube, Maria ; Kittlaus, Kathrin ; Reuter, Gunter ; Maroy, Peter ; Szidonya, Janos ; Rasmuson-Lestander, Åsa ; Ekström, Karin ; Dickson, Barry ; Hugentobler, Christoph ; Stocker, Hugo ; Hafen, Ernst ; Lepesant, Jean Antoine ; Pflugfelder, Gert ; Heisenberg, Martin ; Mechler, Bernard ; Serras, Florenci ; Corominas, Montserrat ; Schneuwly, Stephan ; Preat, Thomas ; Roote, John ; Russell, Steven</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c2058-234b04e6c986f18992b4ed6ad75dd598f731bc59e392c7faf1012d70c7b25b873</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2004</creationdate><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Ryder, Edward</creatorcontrib><creatorcontrib>Blows, Fiona</creatorcontrib><creatorcontrib>Ashburner, Michael</creatorcontrib><creatorcontrib>Bautista-Llacer, Rosa</creatorcontrib><creatorcontrib>Coulson, Darin</creatorcontrib><creatorcontrib>Drummond, Jenny</creatorcontrib><creatorcontrib>Webster, Jane</creatorcontrib><creatorcontrib>Gubb, David</creatorcontrib><creatorcontrib>Gunton, Nicola</creatorcontrib><creatorcontrib>Johnson, Glynnis</creatorcontrib><creatorcontrib>O'Kane, Cahir J</creatorcontrib><creatorcontrib>Huen, David</creatorcontrib><creatorcontrib>Sharma, Punita</creatorcontrib><creatorcontrib>Asztalos, Zoltan</creatorcontrib><creatorcontrib>Baisch, Heiko</creatorcontrib><creatorcontrib>Schulze, Janet</creatorcontrib><creatorcontrib>Kube, Maria</creatorcontrib><creatorcontrib>Kittlaus, Kathrin</creatorcontrib><creatorcontrib>Reuter, Gunter</creatorcontrib><creatorcontrib>Maroy, Peter</creatorcontrib><creatorcontrib>Szidonya, Janos</creatorcontrib><creatorcontrib>Rasmuson-Lestander, Åsa</creatorcontrib><creatorcontrib>Ekström, Karin</creatorcontrib><creatorcontrib>Dickson, Barry</creatorcontrib><creatorcontrib>Hugentobler, Christoph</creatorcontrib><creatorcontrib>Stocker, Hugo</creatorcontrib><creatorcontrib>Hafen, Ernst</creatorcontrib><creatorcontrib>Lepesant, Jean Antoine</creatorcontrib><creatorcontrib>Pflugfelder, Gert</creatorcontrib><creatorcontrib>Heisenberg, Martin</creatorcontrib><creatorcontrib>Mechler, Bernard</creatorcontrib><creatorcontrib>Serras, Florenci</creatorcontrib><creatorcontrib>Corominas, Montserrat</creatorcontrib><creatorcontrib>Schneuwly, Stephan</creatorcontrib><creatorcontrib>Preat, Thomas</creatorcontrib><creatorcontrib>Roote, John</creatorcontrib><creatorcontrib>Russell, Steven</creatorcontrib><collection>CrossRef</collection><jtitle>Genetics (Austin)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Ryder, Edward</au><au>Blows, Fiona</au><au>Ashburner, Michael</au><au>Bautista-Llacer, Rosa</au><au>Coulson, Darin</au><au>Drummond, Jenny</au><au>Webster, Jane</au><au>Gubb, David</au><au>Gunton, Nicola</au><au>Johnson, Glynnis</au><au>O'Kane, Cahir J</au><au>Huen, David</au><au>Sharma, Punita</au><au>Asztalos, Zoltan</au><au>Baisch, Heiko</au><au>Schulze, Janet</au><au>Kube, Maria</au><au>Kittlaus, Kathrin</au><au>Reuter, Gunter</au><au>Maroy, Peter</au><au>Szidonya, Janos</au><au>Rasmuson-Lestander, Åsa</au><au>Ekström, Karin</au><au>Dickson, Barry</au><au>Hugentobler, Christoph</au><au>Stocker, Hugo</au><au>Hafen, Ernst</au><au>Lepesant, Jean Antoine</au><au>Pflugfelder, Gert</au><au>Heisenberg, Martin</au><au>Mechler, Bernard</au><au>Serras, Florenci</au><au>Corominas, Montserrat</au><au>Schneuwly, Stephan</au><au>Preat, Thomas</au><au>Roote, John</au><au>Russell, Steven</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>The DrosDel Collection</atitle><jtitle>Genetics (Austin)</jtitle><date>2004-06-01</date><risdate>2004</risdate><volume>167</volume><issue>2</issue><spage>797</spage><epage>813</epage><pages>797-813</pages><issn>1943-2631</issn><eissn>1943-2631</eissn><abstract>We describe a collection of P-element insertions that have considerable utility for generating custom chromosomal aberrations in Drosophila melanogaster. We have mobilized a pair of engineered P elements, p{RS3} and p{RS5}, to collect 3243 lines unambiguously mapped to the Drosophila genome sequence. The collection contains, on average, an element every 35 kb. We demonstrate the utility of the collection for generating custom chromosomal deletions that have their end points mapped, with base-pair resolution, to the genome sequence. The collection was generated in an isogenic strain, thus affording a uniform background for screens where sensitivity to genetic background is high. The entire collection, along with a computational and genetic toolbox for designing and generating custom deletions, is publicly available. Using the collection it is theoretically possible to generate &gt;12,000 deletions between 1 bp and 1 Mb in size by simple eye color selection. In addition, a further 37,000 deletions, selectable by molecular screening, may be generated. We are now using the collection to generate a second-generation deficiency kit that is precisely mapped to the genome sequence.</abstract><doi>10.1534/genetics.104.026658</doi><tpages>17</tpages><oa>free_for_read</oa></addata></record>
fulltext fulltext
identifier ISSN: 1943-2631
ispartof Genetics (Austin), 2004-06, Vol.167 (2), p.797-813
issn 1943-2631
1943-2631
language eng
recordid cdi_crossref_primary_10_1534_genetics_104_026658
source Oxford Journals【Remote access available】; Free E-Journal (出版社公開部分のみ); Alma/SFX Local Collection
title The DrosDel Collection
url https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2024-12-25T06%3A59%3A44IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-crossref&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=The%20DrosDel%20Collection&rft.jtitle=Genetics%20(Austin)&rft.au=Ryder,%20Edward&rft.date=2004-06-01&rft.volume=167&rft.issue=2&rft.spage=797&rft.epage=813&rft.pages=797-813&rft.issn=1943-2631&rft.eissn=1943-2631&rft_id=info:doi/10.1534/genetics.104.026658&rft_dat=%3Ccrossref%3E10_1534_genetics_104_026658%3C/crossref%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_id=info:pmid/&rfr_iscdi=true