Comparative Study of Extraction Techniques with Ammonia or Methanol/Water for the Isolation of Ginsenosides Using HPLC/MS
Roots of ginseng plants have been extracted with liquid ammonia or methanol/water (60:40 v/v). The extracts were characterized by means of a gradient HPLC/MS method and the results were compared with each other. The ginsenosides Rb , Rb , Rc, Rd, Re, Rf, Rg and Ro including the malonyl ginsenosides...
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| Veröffentlicht in: | Zeitschrift für Naturforschung. B, A journal of chemical sciences A journal of chemical sciences, 2005-02, Vol.60 (2), p.205-210 |
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| container_title | Zeitschrift für Naturforschung. B, A journal of chemical sciences |
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| creator | Nolte, Jürgen Nigge, Walter Hohaus, Eberhard Cho, In-Ho Lentz, Harro |
| description | Roots of ginseng plants have been extracted with liquid ammonia or methanol/water (60:40 v/v). The extracts were characterized by means of a gradient HPLC/MS method and the results were compared with each other. The ginsenosides Rb
, Rb
, Rc, Rd, Re, Rf, Rg
and Ro including the malonyl ginsenosides m-Rb
, m-Rb
, m-Rc and m-Rd could be identified. Contrary to previous reports suggesting that during the extraction with liquid ammonia malonyl ginsenosides are totally converted to neutral ginsenosides, it could now be demonstrated by use of mass spectrometry that even after an extraction time of 4 h malonyl ginsenosides can be detected. However, the amount of malonyl ginsenosides was reduced by about one order of magnitude.
The neutral ginsenosides were not changed by liquid ammonia. The mass spectrometric detection has been performed by use of the quasi-molecule ion [M+Na]
and some specific fragment ions. |
| doi_str_mv | 10.1515/znb-2005-0213 |
| format | Article |
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, Rb
, Rc, Rd, Re, Rf, Rg
and Ro including the malonyl ginsenosides m-Rb
, m-Rb
, m-Rc and m-Rd could be identified. Contrary to previous reports suggesting that during the extraction with liquid ammonia malonyl ginsenosides are totally converted to neutral ginsenosides, it could now be demonstrated by use of mass spectrometry that even after an extraction time of 4 h malonyl ginsenosides can be detected. However, the amount of malonyl ginsenosides was reduced by about one order of magnitude.
The neutral ginsenosides were not changed by liquid ammonia. The mass spectrometric detection has been performed by use of the quasi-molecule ion [M+Na]
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, Rb
, Rc, Rd, Re, Rf, Rg
and Ro including the malonyl ginsenosides m-Rb
, m-Rb
, m-Rc and m-Rd could be identified. Contrary to previous reports suggesting that during the extraction with liquid ammonia malonyl ginsenosides are totally converted to neutral ginsenosides, it could now be demonstrated by use of mass spectrometry that even after an extraction time of 4 h malonyl ginsenosides can be detected. However, the amount of malonyl ginsenosides was reduced by about one order of magnitude.
The neutral ginsenosides were not changed by liquid ammonia. The mass spectrometric detection has been performed by use of the quasi-molecule ion [M+Na]
and some specific fragment ions.</description><subject>Extraction</subject><subject>Ginsenosides</subject><subject>HPLC/MS</subject><subject>Liquid Ammonia Extraction</subject><subject>Methanol/Water</subject><issn>0932-0776</issn><issn>1865-7117</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2005</creationdate><recordtype>article</recordtype><recordid>eNptkM1OwzAQhC0EEuXnyN0vELqOY7sWJxSVFqkIJFpxjLaJQ40au9guUJ6elHLktKvVzGjnI-SKwTUTTAy_3TLLAUQGOeNHZMBGUmSKMXVMBqB5noFS8pScxfgGwHRRwIDsSt9tMGCyH4Y-p22zo76l468UsE7WOzo39crZ962J9NOmFb3tOu8sUh_og0krdH49fMFkAm37U1oZeh_9Gn-9fdLEumicj7bpAxbRulc6fZqVw4fnC3LS4jqay795ThZ343k5zWaPk_vydpbVPB-lzCgNYtQKiQqZ1AKlMIgg1LJpl0WNDeOCa14oXgtpVKGlzkHVCjS0Uo-Qn5PskFsHH2MwbbUJtsOwqxhUe25Vz63ac6v23Hr9zUH_ieu-VmNew3bXL9Wb3wbXf_q_T0Keg-A_YQh1HQ</recordid><startdate>20050201</startdate><enddate>20050201</enddate><creator>Nolte, Jürgen</creator><creator>Nigge, Walter</creator><creator>Hohaus, Eberhard</creator><creator>Cho, In-Ho</creator><creator>Lentz, Harro</creator><general>Verlag der Zeitschrift für Naturforschung</general><scope>AAYXX</scope><scope>CITATION</scope></search><sort><creationdate>20050201</creationdate><title>Comparative Study of Extraction Techniques with Ammonia or Methanol/Water for the Isolation of Ginsenosides Using HPLC/MS</title><author>Nolte, Jürgen ; Nigge, Walter ; Hohaus, Eberhard ; Cho, In-Ho ; Lentz, Harro</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c328t-e79058f56a7a1695a65eaa057bdfb4cad135393473c56e74969207c7090f698a3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2005</creationdate><topic>Extraction</topic><topic>Ginsenosides</topic><topic>HPLC/MS</topic><topic>Liquid Ammonia Extraction</topic><topic>Methanol/Water</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Nolte, Jürgen</creatorcontrib><creatorcontrib>Nigge, Walter</creatorcontrib><creatorcontrib>Hohaus, Eberhard</creatorcontrib><creatorcontrib>Cho, In-Ho</creatorcontrib><creatorcontrib>Lentz, Harro</creatorcontrib><collection>CrossRef</collection><jtitle>Zeitschrift für Naturforschung. B, A journal of chemical sciences</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Nolte, Jürgen</au><au>Nigge, Walter</au><au>Hohaus, Eberhard</au><au>Cho, In-Ho</au><au>Lentz, Harro</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Comparative Study of Extraction Techniques with Ammonia or Methanol/Water for the Isolation of Ginsenosides Using HPLC/MS</atitle><jtitle>Zeitschrift für Naturforschung. B, A journal of chemical sciences</jtitle><date>2005-02-01</date><risdate>2005</risdate><volume>60</volume><issue>2</issue><spage>205</spage><epage>210</epage><pages>205-210</pages><issn>0932-0776</issn><eissn>1865-7117</eissn><abstract>Roots of ginseng plants have been extracted with liquid ammonia or methanol/water (60:40 v/v). The extracts were characterized by means of a gradient HPLC/MS method and the results were compared with each other. The ginsenosides Rb
, Rb
, Rc, Rd, Re, Rf, Rg
and Ro including the malonyl ginsenosides m-Rb
, m-Rb
, m-Rc and m-Rd could be identified. Contrary to previous reports suggesting that during the extraction with liquid ammonia malonyl ginsenosides are totally converted to neutral ginsenosides, it could now be demonstrated by use of mass spectrometry that even after an extraction time of 4 h malonyl ginsenosides can be detected. However, the amount of malonyl ginsenosides was reduced by about one order of magnitude.
The neutral ginsenosides were not changed by liquid ammonia. The mass spectrometric detection has been performed by use of the quasi-molecule ion [M+Na]
and some specific fragment ions.</abstract><pub>Verlag der Zeitschrift für Naturforschung</pub><doi>10.1515/znb-2005-0213</doi><tpages>6</tpages><oa>free_for_read</oa></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0932-0776 |
| ispartof | Zeitschrift für Naturforschung. B, A journal of chemical sciences, 2005-02, Vol.60 (2), p.205-210 |
| issn | 0932-0776 1865-7117 |
| language | eng |
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| source | Alma/SFX Local Collection |
| subjects | Extraction Ginsenosides HPLC/MS Liquid Ammonia Extraction Methanol/Water |
| title | Comparative Study of Extraction Techniques with Ammonia or Methanol/Water for the Isolation of Ginsenosides Using HPLC/MS |
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