The Role of Folic Acid in Rat Embryo Development in a Hypoxic Environment: An Experimental Study
Objective: Folic acid (FA) is a key antioxidant with substantial metabolic roles, and research has demonstrated its effectiveness in reducing congenital anatomical development disorders. This study explores the impact of folic acid on embryo development under hypoxia-induced conditions in embryo cul...
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| Veröffentlicht in: | Journal of Clinical Practice & Research 2024-05, Vol.46 (3), p.251-258 |
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| container_title | Journal of Clinical Practice & Research |
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| creator | Dagli, Ertugrul Nisari, Mehtap Patat, Dilara Cayan, Dicle Atay, Emre Ertekin, Tolga Ucar, Sumeyye |
| description | Objective: Folic acid (FA) is a key antioxidant with substantial metabolic roles, and research has demonstrated its effectiveness in reducing congenital anatomical development disorders. This study explores the impact of folic acid on embryo development under hypoxia-induced conditions in embryo cultures. Materials and Methods: Female Wistar albino rats, aged 4-10 months and weighing 150-250 grams, were utilized for this research. Embryos were extracted from the maternal womb on the 9.5th day of pregnancy. We established six groups, each consisting of 10 embryos: Control (C), Hypoxia (H), 1 mmol FA (1FA), 2 mmol FA (2FA), Hypoxia + 1 mmol FA (H1FA), and Hypoxia + 2 mmol FA (H2FA). Following a 48-hour culture period, the groups were assessed morphologically. Results: When comparing the morphological parameters of the Control and Hypoxia groups, it was statistically demonstrated that the Control group completed its development, whereas the Hypoxia group exhibited insufficient development (p |
| doi_str_mv | 10.14744/cpr.2024.64375 |
| format | Article |
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This study explores the impact of folic acid on embryo development under hypoxia-induced conditions in embryo cultures. Materials and Methods: Female Wistar albino rats, aged 4-10 months and weighing 150-250 grams, were utilized for this research. Embryos were extracted from the maternal womb on the 9.5th day of pregnancy. We established six groups, each consisting of 10 embryos: Control (C), Hypoxia (H), 1 mmol FA (1FA), 2 mmol FA (2FA), Hypoxia + 1 mmol FA (H1FA), and Hypoxia + 2 mmol FA (H2FA). Following a 48-hour culture period, the groups were assessed morphologically. Results: When comparing the morphological parameters of the Control and Hypoxia groups, it was statistically demonstrated that the Control group completed its development, whereas the Hypoxia group exhibited insufficient development (p<0.05). There were statistically significant diferences between the Hypoxia group and the Hypoxia + 1 mmol FA and Hypoxia + 2 mmol FA groups (p<0.05). Both the Hypoxia + 1 mmol FA and Hypoxia + 2 mmol FA groups demonstrated better embryonic development compared to the Hypoxia group (p<0.05). Conclusion: The study has established that FA has positive effects on embryos exposed to hypoxic conditions, which result in developmental delays. Keywords: Folic acid, embryo culture, rat, antioxidant effects.</description><identifier>ISSN: 2980-2156</identifier><identifier>EISSN: 2980-2156</identifier><identifier>DOI: 10.14744/cpr.2024.64375</identifier><language>eng</language><publisher>KARE Publishing</publisher><subject>Analysis ; Animal experimentation ; Embryonic development ; Folic acid ; Influence ; Methods ; Mice ; Testing</subject><ispartof>Journal of Clinical Practice & Research, 2024-05, Vol.46 (3), p.251-258</ispartof><rights>COPYRIGHT 2024 KARE Publishing</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,776,780,860,27901,27902</link.rule.ids></links><search><creatorcontrib>Dagli, Ertugrul</creatorcontrib><creatorcontrib>Nisari, Mehtap</creatorcontrib><creatorcontrib>Patat, Dilara</creatorcontrib><creatorcontrib>Cayan, Dicle</creatorcontrib><creatorcontrib>Atay, Emre</creatorcontrib><creatorcontrib>Ertekin, Tolga</creatorcontrib><creatorcontrib>Ucar, Sumeyye</creatorcontrib><title>The Role of Folic Acid in Rat Embryo Development in a Hypoxic Environment: An Experimental Study</title><title>Journal of Clinical Practice & Research</title><description>Objective: Folic acid (FA) is a key antioxidant with substantial metabolic roles, and research has demonstrated its effectiveness in reducing congenital anatomical development disorders. This study explores the impact of folic acid on embryo development under hypoxia-induced conditions in embryo cultures. Materials and Methods: Female Wistar albino rats, aged 4-10 months and weighing 150-250 grams, were utilized for this research. Embryos were extracted from the maternal womb on the 9.5th day of pregnancy. We established six groups, each consisting of 10 embryos: Control (C), Hypoxia (H), 1 mmol FA (1FA), 2 mmol FA (2FA), Hypoxia + 1 mmol FA (H1FA), and Hypoxia + 2 mmol FA (H2FA). Following a 48-hour culture period, the groups were assessed morphologically. Results: When comparing the morphological parameters of the Control and Hypoxia groups, it was statistically demonstrated that the Control group completed its development, whereas the Hypoxia group exhibited insufficient development (p<0.05). There were statistically significant diferences between the Hypoxia group and the Hypoxia + 1 mmol FA and Hypoxia + 2 mmol FA groups (p<0.05). Both the Hypoxia + 1 mmol FA and Hypoxia + 2 mmol FA groups demonstrated better embryonic development compared to the Hypoxia group (p<0.05). Conclusion: The study has established that FA has positive effects on embryos exposed to hypoxic conditions, which result in developmental delays. Keywords: Folic acid, embryo culture, rat, antioxidant effects.</description><subject>Analysis</subject><subject>Animal experimentation</subject><subject>Embryonic development</subject><subject>Folic acid</subject><subject>Influence</subject><subject>Methods</subject><subject>Mice</subject><subject>Testing</subject><issn>2980-2156</issn><issn>2980-2156</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2024</creationdate><recordtype>article</recordtype><recordid>eNpNkMlOwzAURS0EElXpmq1_IKmn1DG7qKQUqRJSKevgeACjNI6cUJG_x2lZsHrDfffq6QBwj1GKGWdsqbqQEkRYumKUZ1dgRkSOEoKz1fW__hYs-v4LIUQEyzKRz8D74dPAvW8M9BZufOMULJTT0LVwLwdYHuswevhoTqbx3dG0w6RIuB07_xNvy_bkgm8n4QEWLSx_OhPcNMoGvg7ferwDN1Y2vVn81Tl425SH9TbZvTw9r4tdogilQ2I4ldooqRTXEtdcEpILRDFRXFnCNNZW13lGeL4yVuOa5koKQrEmGDOmKZ2D9JL7IRtTudb6IciYF1OPTvnWWBf3BReCcMQZj4blxaCC7_tgbNXFz2UYK4yqM9QqQq0mqNUZKv0FN4tqhQ</recordid><startdate>20240501</startdate><enddate>20240501</enddate><creator>Dagli, Ertugrul</creator><creator>Nisari, Mehtap</creator><creator>Patat, Dilara</creator><creator>Cayan, Dicle</creator><creator>Atay, Emre</creator><creator>Ertekin, Tolga</creator><creator>Ucar, Sumeyye</creator><general>KARE Publishing</general><scope>AAYXX</scope><scope>CITATION</scope><scope>IAO</scope></search><sort><creationdate>20240501</creationdate><title>The Role of Folic Acid in Rat Embryo Development in a Hypoxic Environment: An Experimental Study</title><author>Dagli, Ertugrul ; Nisari, Mehtap ; Patat, Dilara ; Cayan, Dicle ; Atay, Emre ; Ertekin, Tolga ; Ucar, Sumeyye</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c233t-e73adecacc7da1b7a22890312c7cf24d1dfdb852786efd1b38ca9231d21144d33</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2024</creationdate><topic>Analysis</topic><topic>Animal experimentation</topic><topic>Embryonic development</topic><topic>Folic acid</topic><topic>Influence</topic><topic>Methods</topic><topic>Mice</topic><topic>Testing</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Dagli, Ertugrul</creatorcontrib><creatorcontrib>Nisari, Mehtap</creatorcontrib><creatorcontrib>Patat, Dilara</creatorcontrib><creatorcontrib>Cayan, Dicle</creatorcontrib><creatorcontrib>Atay, Emre</creatorcontrib><creatorcontrib>Ertekin, Tolga</creatorcontrib><creatorcontrib>Ucar, Sumeyye</creatorcontrib><collection>CrossRef</collection><collection>Gale Academic OneFile</collection><jtitle>Journal of Clinical Practice & Research</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Dagli, Ertugrul</au><au>Nisari, Mehtap</au><au>Patat, Dilara</au><au>Cayan, Dicle</au><au>Atay, Emre</au><au>Ertekin, Tolga</au><au>Ucar, Sumeyye</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>The Role of Folic Acid in Rat Embryo Development in a Hypoxic Environment: An Experimental Study</atitle><jtitle>Journal of Clinical Practice & Research</jtitle><date>2024-05-01</date><risdate>2024</risdate><volume>46</volume><issue>3</issue><spage>251</spage><epage>258</epage><pages>251-258</pages><issn>2980-2156</issn><eissn>2980-2156</eissn><abstract>Objective: Folic acid (FA) is a key antioxidant with substantial metabolic roles, and research has demonstrated its effectiveness in reducing congenital anatomical development disorders. This study explores the impact of folic acid on embryo development under hypoxia-induced conditions in embryo cultures. Materials and Methods: Female Wistar albino rats, aged 4-10 months and weighing 150-250 grams, were utilized for this research. Embryos were extracted from the maternal womb on the 9.5th day of pregnancy. We established six groups, each consisting of 10 embryos: Control (C), Hypoxia (H), 1 mmol FA (1FA), 2 mmol FA (2FA), Hypoxia + 1 mmol FA (H1FA), and Hypoxia + 2 mmol FA (H2FA). Following a 48-hour culture period, the groups were assessed morphologically. Results: When comparing the morphological parameters of the Control and Hypoxia groups, it was statistically demonstrated that the Control group completed its development, whereas the Hypoxia group exhibited insufficient development (p<0.05). There were statistically significant diferences between the Hypoxia group and the Hypoxia + 1 mmol FA and Hypoxia + 2 mmol FA groups (p<0.05). Both the Hypoxia + 1 mmol FA and Hypoxia + 2 mmol FA groups demonstrated better embryonic development compared to the Hypoxia group (p<0.05). Conclusion: The study has established that FA has positive effects on embryos exposed to hypoxic conditions, which result in developmental delays. Keywords: Folic acid, embryo culture, rat, antioxidant effects.</abstract><pub>KARE Publishing</pub><doi>10.14744/cpr.2024.64375</doi><tpages>8</tpages><oa>free_for_read</oa></addata></record> |
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| source | DOAJ Directory of Open Access Journals; Alma/SFX Local Collection; ProQuest Central |
| subjects | Analysis Animal experimentation Embryonic development Folic acid Influence Methods Mice Testing |
| title | The Role of Folic Acid in Rat Embryo Development in a Hypoxic Environment: An Experimental Study |
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