Introduction of Tyramide Signal Amplification (TSA) to Pre-embedding Nanogold-Silver Staining at the Electron Microscopic Level
The tyramide signal amplification (TSA) technique has been shown to detect scarce tissue antigens in light and electron microscopy. In this study we applied the TSA technique at the electron microscopic level to pre-embedding immunocytochemistry. This protocol was compared to the non-amplified proto...
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| Veröffentlicht in: | The journal of histochemistry and cytochemistry 2005-02, Vol.53 (2), p.249-252 |
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| container_title | The journal of histochemistry and cytochemistry |
| container_volume | 53 |
| creator | Lee, Seung-won Lee, Song Eun Ko, Seong Hyuk Hong, Eun Kyoung Nam, Kwang Il Nakamura, Kei-ichiro Imayama, Shuhei Park, Yeong-Joon Ahn, Kyu Youn Bae, Choon Sang Kim, Baik Yoon Park, Sung Sik |
| description | The tyramide signal amplification (TSA) technique has been shown to detect scarce tissue antigens in light and electron microscopy. In this study we applied the TSA technique at the electron microscopic level to pre-embedding immunocytochemistry. This protocol was compared to the non-amplified protocol. With the TSA protocol, the labeling of GM130, a cis-Golgi matrix protein, was tested in a cell line and found to be highly sensitive and more enhanced than that with the simple protocol. Moreover, the gold particles were well localized to the cis-side of the Golgi apparatus in both the TSA and the simple protocol. |
| doi_str_mv | 10.1369/jhc.3B6194.2005 |
| format | Article |
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In this study we applied the TSA technique at the electron microscopic level to pre-embedding immunocytochemistry. This protocol was compared to the non-amplified protocol. With the TSA protocol, the labeling of GM130, a cis-Golgi matrix protein, was tested in a cell line and found to be highly sensitive and more enhanced than that with the simple protocol. Moreover, the gold particles were well localized to the cis-side of the Golgi apparatus in both the TSA and the simple protocol.</description><identifier>ISSN: 0022-1554</identifier><identifier>EISSN: 1551-5044</identifier><identifier>DOI: 10.1369/jhc.3B6194.2005</identifier><identifier>PMID: 15684338</identifier><language>eng</language><publisher>Los Angeles, CA: Histochemical Soc</publisher><subject>Autoantigens ; Cell Line, Tumor ; Gold ; Golgi Apparatus - metabolism ; Humans ; Immunohistochemistry - methods ; Melanoma ; Membrane Proteins - metabolism ; Microscopy, Electron ; Silver Staining ; Tyramine</subject><ispartof>The journal of histochemistry and cytochemistry, 2005-02, Vol.53 (2), p.249-252</ispartof><rights>2005 Authors</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c438t-958dfc5d837a43e2ba4a6bd5a5795624790cf37d201d19b50afdc38b6e4fe5f43</citedby><cites>FETCH-LOGICAL-c438t-958dfc5d837a43e2ba4a6bd5a5795624790cf37d201d19b50afdc38b6e4fe5f43</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://journals.sagepub.com/doi/pdf/10.1369/jhc.3B6194.2005$$EPDF$$P50$$Gsage$$H</linktopdf><linktohtml>$$Uhttps://journals.sagepub.com/doi/10.1369/jhc.3B6194.2005$$EHTML$$P50$$Gsage$$H</linktohtml><link.rule.ids>314,776,780,21798,27901,27902,43597,43598</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/15684338$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Lee, Seung-won</creatorcontrib><creatorcontrib>Lee, Song Eun</creatorcontrib><creatorcontrib>Ko, Seong Hyuk</creatorcontrib><creatorcontrib>Hong, Eun Kyoung</creatorcontrib><creatorcontrib>Nam, Kwang Il</creatorcontrib><creatorcontrib>Nakamura, Kei-ichiro</creatorcontrib><creatorcontrib>Imayama, Shuhei</creatorcontrib><creatorcontrib>Park, Yeong-Joon</creatorcontrib><creatorcontrib>Ahn, Kyu Youn</creatorcontrib><creatorcontrib>Bae, Choon Sang</creatorcontrib><creatorcontrib>Kim, Baik Yoon</creatorcontrib><creatorcontrib>Park, Sung Sik</creatorcontrib><title>Introduction of Tyramide Signal Amplification (TSA) to Pre-embedding Nanogold-Silver Staining at the Electron Microscopic Level</title><title>The journal of histochemistry and cytochemistry</title><addtitle>J Histochem Cytochem</addtitle><description>The tyramide signal amplification (TSA) technique has been shown to detect scarce tissue antigens in light and electron microscopy. In this study we applied the TSA technique at the electron microscopic level to pre-embedding immunocytochemistry. This protocol was compared to the non-amplified protocol. With the TSA protocol, the labeling of GM130, a cis-Golgi matrix protein, was tested in a cell line and found to be highly sensitive and more enhanced than that with the simple protocol. 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In this study we applied the TSA technique at the electron microscopic level to pre-embedding immunocytochemistry. This protocol was compared to the non-amplified protocol. With the TSA protocol, the labeling of GM130, a cis-Golgi matrix protein, was tested in a cell line and found to be highly sensitive and more enhanced than that with the simple protocol. Moreover, the gold particles were well localized to the cis-side of the Golgi apparatus in both the TSA and the simple protocol.</abstract><cop>Los Angeles, CA</cop><pub>Histochemical Soc</pub><pmid>15684338</pmid><doi>10.1369/jhc.3B6194.2005</doi><tpages>4</tpages><oa>free_for_read</oa></addata></record> |
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| subjects | Autoantigens Cell Line, Tumor Gold Golgi Apparatus - metabolism Humans Immunohistochemistry - methods Melanoma Membrane Proteins - metabolism Microscopy, Electron Silver Staining Tyramine |
| title | Introduction of Tyramide Signal Amplification (TSA) to Pre-embedding Nanogold-Silver Staining at the Electron Microscopic Level |
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