Isolation and characterization of a strain of Bacillus thuringiensis serovar kenyae encoding olny δ-endotoxin Cry1E

Isolation and characterization of a strain of Bacillus thuringiensis serovar kenyae encoding olny δ-endotoxin Cry1E

Bacillus thuringiensis STB-3 which was isolated from a Korean soil sample showed high toxicity to Spodoptera exigua. Its crystal toxin was bipyramidal-shaped with a molecular weight of 130 kDa. The H-serotype of STB-3 was identical to B. thuringiensis serovar kenaye (H4a4c). However, PCR analysis us...

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Veröffentlicht in:Applied Entomology and Zoology 1999/08/25, Vol.34(3), pp.379-382
Hauptverfasser: Chang, Jim-Hee, Je, Yeon-Ho, Roh, Jong-Yul, Park, Hyun-Woo, Jin, Byung-Rae, Lee, Dae-Weon, Kim, Sang-Hyun, Yang, Zi-Wen, Kang, Seok-Kwon
Format: Artikel
Sprache:eng
Schlagworte:
Bacillus thuringiensis
kenyae, δ-endotoxin, cry1E, Spodoptera exigua
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Zusammenfassung:Bacillus thuringiensis STB-3 which was isolated from a Korean soil sample showed high toxicity to Spodoptera exigua. Its crystal toxin was bipyramidal-shaped with a molecular weight of 130 kDa. The H-serotype of STB-3 was identical to B. thuringiensis serovar kenaye (H4a4c). However, PCR analysis using Spodoptera-specific cry gene primers showed that STB-3 had only the cry1E crystal gene, differing from that of serovar kenyae (reference strain) which contains cry1Ab, cry1Ac and cry1E crystal genes.
ISSN:0003-6862
1347-605X
DOI:10.1303/aez.34.379