Occurrence of Lewis a epitope in N-glycans of a glycoallergen, Jun a 1, from mountain cedar (Juniperus ashei) pollen
We have determined the structures of N-glycans linked to major allergens in the mountain cedar (Juniperus ashei) pollen, Jun a 1. First, two kinds of the pollen glycoallergen (Jun a 1-A and Jun a l-B) were purified from partially purified Jun a 1 by cation exchange chromatography. The N-glycans were...
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| Veröffentlicht in: | Bioscience, biotechnology, and biochemistry biotechnology, and biochemistry, 2005-01, Vol.69 (1), p.137-144 |
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| container_title | Bioscience, biotechnology, and biochemistry |
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| creator | Kimura, Y. (Okayama Univ. (Japan). Faculty of Agriculture) Kamamoto, M Maeda, M Okano, M Yokoyama, M Kino, K |
| description | We have determined the structures of N-glycans linked to major allergens in the mountain cedar (Juniperus ashei) pollen, Jun a 1. First, two kinds of the pollen glycoallergen (Jun a 1-A and Jun a l-B) were purified from partially purified Jun a 1 by cation exchange chromatography. The N-glycans were liberated by hydrazinolysis from the two glycoallergens and the resulting sugar chains were N-acetylated and then coupled with 2-aminopyridine. Three pyridylaminated sugar chains were purified by reversed-phase HPLC and size-fractionation HPLC from Jun a 1-A and Jun a l-B respectively. The structures were determined by a combination of exo- and endo-glycosidase digestions, two dimensional sugar chain mapping, and electrospray ionization mass spectrometry (ESI-MS) analysis. Structural analysis indicated that Lewis a epitope (GalBetal-3(Fucalpha1-4)GlcNAcBetal-) occurs in the N-glycans of the pollen allergens. |
| doi_str_mv | 10.1271/bbb.69.137 |
| format | Article |
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(Okayama Univ. (Japan). Faculty of Agriculture) ; Kamamoto, M ; Maeda, M ; Okano, M ; Yokoyama, M ; Kino, K</creator><creatorcontrib>Kimura, Y. (Okayama Univ. (Japan). Faculty of Agriculture) ; Kamamoto, M ; Maeda, M ; Okano, M ; Yokoyama, M ; Kino, K</creatorcontrib><description>We have determined the structures of N-glycans linked to major allergens in the mountain cedar (Juniperus ashei) pollen, Jun a 1. First, two kinds of the pollen glycoallergen (Jun a 1-A and Jun a l-B) were purified from partially purified Jun a 1 by cation exchange chromatography. The N-glycans were liberated by hydrazinolysis from the two glycoallergens and the resulting sugar chains were N-acetylated and then coupled with 2-aminopyridine. Three pyridylaminated sugar chains were purified by reversed-phase HPLC and size-fractionation HPLC from Jun a 1-A and Jun a l-B respectively. The structures were determined by a combination of exo- and endo-glycosidase digestions, two dimensional sugar chain mapping, and electrospray ionization mass spectrometry (ESI-MS) analysis. Structural analysis indicated that Lewis a epitope (GalBetal-3(Fucalpha1-4)GlcNAcBetal-) occurs in the N-glycans of the pollen allergens.</description><identifier>ISSN: 0916-8451</identifier><identifier>EISSN: 1347-6947</identifier><identifier>DOI: 10.1271/bbb.69.137</identifier><identifier>PMID: 15665478</identifier><language>eng</language><publisher>Tokyo: Japan Society for Bioscience, Biotechnology, and Agrochemistry</publisher><subject>ALLERGENS ; Allergens - chemistry ; ANTIGENS ; Antigens, Plant ; Biological and medical sciences ; BLOOD GROUPS ; Carbohydrate Sequence ; Epitopes - analysis ; Fundamental and applied biological sciences. Psychology ; HPLC ; HYPERSENSITIVITY ; JUNIPERUS ; Juniperus - chemistry ; Juniperus - immunology ; Juniperus ashei ; Lewis a epitope ; Molecular Sequence Data ; mountain cedar pollen ; N-glycan ; Oligosaccharides - analysis ; Oligosaccharides - immunology ; Plant Proteins - chemistry ; POLLEN ; Pollen - chemistry ; Pollen - immunology ; pollen allergen ; POLYSACCHARIDES ; Polysaccharides - chemistry ; Polysaccharides - immunology</subject><ispartof>Bioscience, biotechnology, and biochemistry, 2005-01, Vol.69 (1), p.137-144</ispartof><rights>2005 by Japan Society for Bioscience, Biotechnology, and Agrochemistry 2005</rights><rights>2005 INIST-CNRS</rights><rights>Copyright Japan Science and Technology Agency 2005</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c627t-d4e2d18acc5986d4cacc641bcac7e3a34b0f5f6cb923230d7b19063fd52828773</citedby><cites>FETCH-LOGICAL-c627t-d4e2d18acc5986d4cacc641bcac7e3a34b0f5f6cb923230d7b19063fd52828773</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,776,780,27901,27902</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=16733338$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/15665478$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Kimura, Y. (Okayama Univ. (Japan). Faculty of Agriculture)</creatorcontrib><creatorcontrib>Kamamoto, M</creatorcontrib><creatorcontrib>Maeda, M</creatorcontrib><creatorcontrib>Okano, M</creatorcontrib><creatorcontrib>Yokoyama, M</creatorcontrib><creatorcontrib>Kino, K</creatorcontrib><title>Occurrence of Lewis a epitope in N-glycans of a glycoallergen, Jun a 1, from mountain cedar (Juniperus ashei) pollen</title><title>Bioscience, biotechnology, and biochemistry</title><addtitle>Biosci Biotechnol Biochem</addtitle><description>We have determined the structures of N-glycans linked to major allergens in the mountain cedar (Juniperus ashei) pollen, Jun a 1. First, two kinds of the pollen glycoallergen (Jun a 1-A and Jun a l-B) were purified from partially purified Jun a 1 by cation exchange chromatography. The N-glycans were liberated by hydrazinolysis from the two glycoallergens and the resulting sugar chains were N-acetylated and then coupled with 2-aminopyridine. Three pyridylaminated sugar chains were purified by reversed-phase HPLC and size-fractionation HPLC from Jun a 1-A and Jun a l-B respectively. The structures were determined by a combination of exo- and endo-glycosidase digestions, two dimensional sugar chain mapping, and electrospray ionization mass spectrometry (ESI-MS) analysis. Structural analysis indicated that Lewis a epitope (GalBetal-3(Fucalpha1-4)GlcNAcBetal-) occurs in the N-glycans of the pollen allergens.</description><subject>ALLERGENS</subject><subject>Allergens - chemistry</subject><subject>ANTIGENS</subject><subject>Antigens, Plant</subject><subject>Biological and medical sciences</subject><subject>BLOOD GROUPS</subject><subject>Carbohydrate Sequence</subject><subject>Epitopes - analysis</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>HPLC</subject><subject>HYPERSENSITIVITY</subject><subject>JUNIPERUS</subject><subject>Juniperus - chemistry</subject><subject>Juniperus - immunology</subject><subject>Juniperus ashei</subject><subject>Lewis a epitope</subject><subject>Molecular Sequence Data</subject><subject>mountain cedar pollen</subject><subject>N-glycan</subject><subject>Oligosaccharides - analysis</subject><subject>Oligosaccharides - immunology</subject><subject>Plant Proteins - chemistry</subject><subject>POLLEN</subject><subject>Pollen - chemistry</subject><subject>Pollen - immunology</subject><subject>pollen allergen</subject><subject>POLYSACCHARIDES</subject><subject>Polysaccharides - chemistry</subject><subject>Polysaccharides - immunology</subject><issn>0916-8451</issn><issn>1347-6947</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2005</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNptkd9rFDEQx4Mo9mx98V0JiKLSPfM7u49StLUcrQ_6HLLZ5Nyym2yTXcr9985xJwUxBGYy8_l-GTIIvaJkTZmmn9u2XatmTbl-glaUC12pRuinaEUaqqpaSHqCXpRyRwgUJH2OTqhUSgpdr9B869ySs4_O4xTwxj_0BVvsp35Ok8d9xDfVdtg5G8u-b_H-keww-Lz18RxfLxGK9ByHnEY8piXOFkTOdzbjD9DtJ58XsCy_ff8RTwmU8Qw9C3Yo_uUxnqJf377-vLiqNreX3y--bCqnmJ6rTnjW0do6J5tadcJBpgRtIWrPLRctCTIo1zaMM0463dKGKB46yWpWa81P0fuD75TT_eLLbMa-OD8MNvq0FKM017RhEsC3_4B3ackRZjNUiKamTFIC1KcD5XIqJftgptyPNu8MJWa_CQObMKoxsAmA3xwtl3b03SN6_HoA3h0BW5wdQrbR9eWRg-Hg7Dl54PoYUh7tQ8pDZ2a7G1L-K-L_HeD1QRdsMnabAbv-wQiRcJng_A9I_aqr</recordid><startdate>20050101</startdate><enddate>20050101</enddate><creator>Kimura, Y. (Okayama Univ. (Japan). Faculty of Agriculture)</creator><creator>Kamamoto, M</creator><creator>Maeda, M</creator><creator>Okano, M</creator><creator>Yokoyama, M</creator><creator>Kino, K</creator><general>Japan Society for Bioscience, Biotechnology, and Agrochemistry</general><general>Japan Society for Bioscience Biotechnology and Agrochemistry</general><general>Oxford University Press</general><scope>FBQ</scope><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>20050101</creationdate><title>Occurrence of Lewis a epitope in N-glycans of a glycoallergen, Jun a 1, from mountain cedar (Juniperus ashei) pollen</title><author>Kimura, Y. (Okayama Univ. (Japan). Faculty of Agriculture) ; Kamamoto, M ; Maeda, M ; Okano, M ; Yokoyama, M ; Kino, K</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c627t-d4e2d18acc5986d4cacc641bcac7e3a34b0f5f6cb923230d7b19063fd52828773</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2005</creationdate><topic>ALLERGENS</topic><topic>Allergens - chemistry</topic><topic>ANTIGENS</topic><topic>Antigens, Plant</topic><topic>Biological and medical sciences</topic><topic>BLOOD GROUPS</topic><topic>Carbohydrate Sequence</topic><topic>Epitopes - analysis</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>HPLC</topic><topic>HYPERSENSITIVITY</topic><topic>JUNIPERUS</topic><topic>Juniperus - chemistry</topic><topic>Juniperus - immunology</topic><topic>Juniperus ashei</topic><topic>Lewis a epitope</topic><topic>Molecular Sequence Data</topic><topic>mountain cedar pollen</topic><topic>N-glycan</topic><topic>Oligosaccharides - analysis</topic><topic>Oligosaccharides - immunology</topic><topic>Plant Proteins - chemistry</topic><topic>POLLEN</topic><topic>Pollen - chemistry</topic><topic>Pollen - immunology</topic><topic>pollen allergen</topic><topic>POLYSACCHARIDES</topic><topic>Polysaccharides - chemistry</topic><topic>Polysaccharides - immunology</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Kimura, Y. (Okayama Univ. (Japan). Faculty of Agriculture)</creatorcontrib><creatorcontrib>Kamamoto, M</creatorcontrib><creatorcontrib>Maeda, M</creatorcontrib><creatorcontrib>Okano, M</creatorcontrib><creatorcontrib>Yokoyama, M</creatorcontrib><creatorcontrib>Kino, K</creatorcontrib><collection>AGRIS</collection><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Bioscience, biotechnology, and biochemistry</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Kimura, Y. (Okayama Univ. (Japan). Faculty of Agriculture)</au><au>Kamamoto, M</au><au>Maeda, M</au><au>Okano, M</au><au>Yokoyama, M</au><au>Kino, K</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Occurrence of Lewis a epitope in N-glycans of a glycoallergen, Jun a 1, from mountain cedar (Juniperus ashei) pollen</atitle><jtitle>Bioscience, biotechnology, and biochemistry</jtitle><addtitle>Biosci Biotechnol Biochem</addtitle><date>2005-01-01</date><risdate>2005</risdate><volume>69</volume><issue>1</issue><spage>137</spage><epage>144</epage><pages>137-144</pages><issn>0916-8451</issn><eissn>1347-6947</eissn><abstract>We have determined the structures of N-glycans linked to major allergens in the mountain cedar (Juniperus ashei) pollen, Jun a 1. First, two kinds of the pollen glycoallergen (Jun a 1-A and Jun a l-B) were purified from partially purified Jun a 1 by cation exchange chromatography. The N-glycans were liberated by hydrazinolysis from the two glycoallergens and the resulting sugar chains were N-acetylated and then coupled with 2-aminopyridine. Three pyridylaminated sugar chains were purified by reversed-phase HPLC and size-fractionation HPLC from Jun a 1-A and Jun a l-B respectively. The structures were determined by a combination of exo- and endo-glycosidase digestions, two dimensional sugar chain mapping, and electrospray ionization mass spectrometry (ESI-MS) analysis. Structural analysis indicated that Lewis a epitope (GalBetal-3(Fucalpha1-4)GlcNAcBetal-) occurs in the N-glycans of the pollen allergens.</abstract><cop>Tokyo</cop><pub>Japan Society for Bioscience, Biotechnology, and Agrochemistry</pub><pmid>15665478</pmid><doi>10.1271/bbb.69.137</doi><tpages>8</tpages></addata></record> |
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| source | J-STAGE Free; Oxford University Press Journals All Titles (1996-Current); MEDLINE; Freely Accessible Japanese Titles; EZB-FREE-00999 freely available EZB journals; Free Full-Text Journals in Chemistry |
| subjects | ALLERGENS Allergens - chemistry ANTIGENS Antigens, Plant Biological and medical sciences BLOOD GROUPS Carbohydrate Sequence Epitopes - analysis Fundamental and applied biological sciences. Psychology HPLC HYPERSENSITIVITY JUNIPERUS Juniperus - chemistry Juniperus - immunology Juniperus ashei Lewis a epitope Molecular Sequence Data mountain cedar pollen N-glycan Oligosaccharides - analysis Oligosaccharides - immunology Plant Proteins - chemistry POLLEN Pollen - chemistry Pollen - immunology pollen allergen POLYSACCHARIDES Polysaccharides - chemistry Polysaccharides - immunology |
| title | Occurrence of Lewis a epitope in N-glycans of a glycoallergen, Jun a 1, from mountain cedar (Juniperus ashei) pollen |
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