Direct evidence of interaction of a green tea polyphenol, epigallocatechin gallate, with lipid bilayers by solid-state nuclear magnetic resonance
The interaction of a tea catechin, epigallocatechin gallate (EGCg), with the model membrane of dimyristoylphosphatidylcholine (DMPC) was studied by solidstate 31P and 2H NMR. The 31P chemical shift anisotropy of the DMPC phosphate group decreased on addition of EGCg. The 2H NMR spectrum of [4-2H] EG...
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| Veröffentlicht in: | Bioscience, biotechnology, and biochemistry biotechnology, and biochemistry, 2004-08, Vol.68 (8), p.1743-1747 |
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| container_title | Bioscience, biotechnology, and biochemistry |
| container_volume | 68 |
| creator | Kumazawa, S. (Shizuoka Univ. (Japan)) Kajiya, K Naito, A Saito, H Tuzi, S Tanio, M Suzuki, M Nanjo, F Suzuki, E Nakayama, T |
| description | The interaction of a tea catechin, epigallocatechin gallate (EGCg), with the model membrane of dimyristoylphosphatidylcholine (DMPC) was studied by solidstate 31P and 2H NMR. The 31P chemical shift anisotropy of the DMPC phosphate group decreased on addition of EGCg. The 2H NMR spectrum of [4-2H] EGCg, which is deuterated at the 4-position, in the DMPC liposomes gave deuterium nuclei with much smaller quadrupole splittings than those in the solid phase. These 31P and 2H NMR observations provide direct experimental evidence that the EGCg molecule interacts with the lipid bilayers. |
| doi_str_mv | 10.1271/bbb.68.1743 |
| format | Article |
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(Shizuoka Univ. (Japan)) ; Kajiya, K ; Naito, A ; Saito, H ; Tuzi, S ; Tanio, M ; Suzuki, M ; Nanjo, F ; Suzuki, E ; Nakayama, T</creator><creatorcontrib>Kumazawa, S. (Shizuoka Univ. (Japan)) ; Kajiya, K ; Naito, A ; Saito, H ; Tuzi, S ; Tanio, M ; Suzuki, M ; Nanjo, F ; Suzuki, E ; Nakayama, T</creatorcontrib><description>The interaction of a tea catechin, epigallocatechin gallate (EGCg), with the model membrane of dimyristoylphosphatidylcholine (DMPC) was studied by solidstate 31P and 2H NMR. The 31P chemical shift anisotropy of the DMPC phosphate group decreased on addition of EGCg. The 2H NMR spectrum of [4-2H] EGCg, which is deuterated at the 4-position, in the DMPC liposomes gave deuterium nuclei with much smaller quadrupole splittings than those in the solid phase. These 31P and 2H NMR observations provide direct experimental evidence that the EGCg molecule interacts with the lipid bilayers.</description><identifier>ISSN: 0916-8451</identifier><identifier>EISSN: 1347-6947</identifier><identifier>DOI: 10.1271/bbb.68.1743</identifier><identifier>PMID: 15322359</identifier><language>eng</language><publisher>Tokyo: Japan Society for Bioscience, Biotechnology, and Agrochemistry</publisher><subject>ANALYTICAL METHODS ; Biological and medical sciences ; CAMELLIA SINENSIS ; CATECHIN ; Catechin - analogs & derivatives ; Catechin - chemistry ; Deuterium - chemistry ; Dimyristoylphosphatidylcholine - chemistry ; epigallocatechin gallate ; Flavonoids - chemistry ; Fundamental and applied biological sciences. Psychology ; lipid bilayer ; Lipid Bilayers - chemistry ; LIPIDS ; liposome ; Magnetic Resonance Spectroscopy ; NMR SPECTROSCOPY ; PHENOLIC COMPOUNDS ; Phenols - chemistry ; Polyphenols ; solid-state NMR ; TEA ; Tea - chemistry ; tea catechins</subject><ispartof>Bioscience, biotechnology, and biochemistry, 2004-08, Vol.68 (8), p.1743-1747</ispartof><rights>2004 by Japan Society for Bioscience, Biotechnology, and Agrochemistry 2004</rights><rights>2004 INIST-CNRS</rights><rights>Copyright Japan Science and Technology Agency 2004</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c539t-428a84b07d4989da0e3506ea0abeb51e95b30ff889c2ac5816134c89504abda53</citedby><cites>FETCH-LOGICAL-c539t-428a84b07d4989da0e3506ea0abeb51e95b30ff889c2ac5816134c89504abda53</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,776,780,27901,27902</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=16238435$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/15322359$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Kumazawa, S. 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The 2H NMR spectrum of [4-2H] EGCg, which is deuterated at the 4-position, in the DMPC liposomes gave deuterium nuclei with much smaller quadrupole splittings than those in the solid phase. These 31P and 2H NMR observations provide direct experimental evidence that the EGCg molecule interacts with the lipid bilayers.</description><subject>ANALYTICAL METHODS</subject><subject>Biological and medical sciences</subject><subject>CAMELLIA SINENSIS</subject><subject>CATECHIN</subject><subject>Catechin - analogs & derivatives</subject><subject>Catechin - chemistry</subject><subject>Deuterium - chemistry</subject><subject>Dimyristoylphosphatidylcholine - chemistry</subject><subject>epigallocatechin gallate</subject><subject>Flavonoids - chemistry</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>lipid bilayer</subject><subject>Lipid Bilayers - chemistry</subject><subject>LIPIDS</subject><subject>liposome</subject><subject>Magnetic Resonance Spectroscopy</subject><subject>NMR SPECTROSCOPY</subject><subject>PHENOLIC COMPOUNDS</subject><subject>Phenols - chemistry</subject><subject>Polyphenols</subject><subject>solid-state NMR</subject><subject>TEA</subject><subject>Tea - chemistry</subject><subject>tea catechins</subject><issn>0916-8451</issn><issn>1347-6947</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2004</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNptkU2LFDEQhhtR3HX15FkJiF7cHpPOR6ePsn6zoAc9N5V09UyWTNImGZf5Gf5jM8zIgniqqvBU5a16m-YpoyvW9eyNMWal9Ir1gt9rzhkXfasG0d9vzunAVKuFZGfNo5xvKK0Pkj1szpjkXcflcN78fucS2kLwl5swWCRxJi4UTGCLi-FQAlknxEAKAlmi3y8bDNFfElzcGryPFgrajQvkUNX8kty6siHeLW4ixnnYY8rE7EmO3k1tLpUhYWc9QiJbWAcszpKEOQaoCh43D2bwGZ-c4kXz48P771ef2uuvHz9fvb1ureRDaUWnQQtD-0kMepiAIpdUIVAwaCTDQRpO51nrwXZgpWaqXsbqQVIBZgLJL5pXx7lLij93mMu4ddliXSFg3OVRqX4YhKIVfPEPeBN3KVRtIxP1c0aFUJV6faRsijknnMcluS2k_cjoePBprD6NSo8Hnyr9_DRzZ7Y43bEnYyrw8gRAtuDnVE_j8h2nOq4FP2yhjpwLc0xbuI3JT2OBvY_pbxP_v4Jnx8YZ4gjrVLkv3zpKJaW8Ez3_A94rt_0</recordid><startdate>20040801</startdate><enddate>20040801</enddate><creator>Kumazawa, S. 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Psychology</topic><topic>lipid bilayer</topic><topic>Lipid Bilayers - chemistry</topic><topic>LIPIDS</topic><topic>liposome</topic><topic>Magnetic Resonance Spectroscopy</topic><topic>NMR SPECTROSCOPY</topic><topic>PHENOLIC COMPOUNDS</topic><topic>Phenols - chemistry</topic><topic>Polyphenols</topic><topic>solid-state NMR</topic><topic>TEA</topic><topic>Tea - chemistry</topic><topic>tea catechins</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Kumazawa, S. (Shizuoka Univ. 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The 2H NMR spectrum of [4-2H] EGCg, which is deuterated at the 4-position, in the DMPC liposomes gave deuterium nuclei with much smaller quadrupole splittings than those in the solid phase. These 31P and 2H NMR observations provide direct experimental evidence that the EGCg molecule interacts with the lipid bilayers.</abstract><cop>Tokyo</cop><pub>Japan Society for Bioscience, Biotechnology, and Agrochemistry</pub><pmid>15322359</pmid><doi>10.1271/bbb.68.1743</doi><tpages>5</tpages><oa>free_for_read</oa></addata></record> |
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| source | J-STAGE Free; Oxford University Press Journals All Titles (1996-Current); MEDLINE; Freely Accessible Japanese Titles; Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals; Free Full-Text Journals in Chemistry |
| subjects | ANALYTICAL METHODS Biological and medical sciences CAMELLIA SINENSIS CATECHIN Catechin - analogs & derivatives Catechin - chemistry Deuterium - chemistry Dimyristoylphosphatidylcholine - chemistry epigallocatechin gallate Flavonoids - chemistry Fundamental and applied biological sciences. Psychology lipid bilayer Lipid Bilayers - chemistry LIPIDS liposome Magnetic Resonance Spectroscopy NMR SPECTROSCOPY PHENOLIC COMPOUNDS Phenols - chemistry Polyphenols solid-state NMR TEA Tea - chemistry tea catechins |
| title | Direct evidence of interaction of a green tea polyphenol, epigallocatechin gallate, with lipid bilayers by solid-state nuclear magnetic resonance |
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