Reactivities of mutants of a major house dust mite allergen Der f 2 to mouse anti-Der f 2 monoclonal antibodies analyzed by immunoblotting
A total of sixteen recombinant variants of a major house dust mite allergen Der f 2, the wild-type Der f 2, six cysteine mutants, six proline mutants, and three lysine mutants, were expressed in Escherichia coli. The cells were solubilized and run on SDS-PAGE under reducing conditions. Epitopes for...
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| Veröffentlicht in: | Bioscience, biotechnology, and biochemistry biotechnology, and biochemistry, 2001-03, Vol.65 (3), p.694-697 |
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| container_title | Bioscience, biotechnology, and biochemistry |
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| creator | Takai, T. (Asahi Breweries Co. Ltd., Moriya, Ibaraki (Japan)) Akagawa Chihara, M Yokota, T Okumura, Y |
| description | A total of sixteen recombinant variants of a major house dust mite allergen Der f 2, the wild-type Der f 2, six cysteine mutants, six proline mutants, and three lysine mutants, were expressed in Escherichia coli. The cells were solubilized and run on SDS-PAGE under reducing conditions. Epitopes for five mouse anti-Der f 2 monoclonal antibodies, 1B2, 7C10, 13A4, 15E11, and 18G8, to the recombinant Der f 2 variants were characterized by immunoblot analysis. |
| doi_str_mv | 10.1271/bbb.65.694 |
| format | Article |
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(Asahi Breweries Co. Ltd., Moriya, Ibaraki (Japan)) ; Akagawa Chihara, M ; Yokota, T ; Okumura, Y</creator><creatorcontrib>Takai, T. (Asahi Breweries Co. Ltd., Moriya, Ibaraki (Japan)) ; Akagawa Chihara, M ; Yokota, T ; Okumura, Y</creatorcontrib><description>A total of sixteen recombinant variants of a major house dust mite allergen Der f 2, the wild-type Der f 2, six cysteine mutants, six proline mutants, and three lysine mutants, were expressed in Escherichia coli. The cells were solubilized and run on SDS-PAGE under reducing conditions. Epitopes for five mouse anti-Der f 2 monoclonal antibodies, 1B2, 7C10, 13A4, 15E11, and 18G8, to the recombinant Der f 2 variants were characterized by immunoblot analysis.</description><identifier>ISSN: 0916-8451</identifier><identifier>EISSN: 1347-6947</identifier><identifier>DOI: 10.1271/bbb.65.694</identifier><identifier>PMID: 11330693</identifier><language>eng</language><publisher>Tokyo: Japan Society for Bioscience, Biotechnology, and Agrochemistry</publisher><subject>ALLERGENS ; Allergens - genetics ; Allergens - immunology ; Animals ; Antibodies, Monoclonal - immunology ; Antigenic determinants, haptens, artificial antigens ; Antigens ; Antigens, Dermatophagoides ; Biological and medical sciences ; Der f 2 ; epitope ; Epitope Mapping ; Epitopes, B-Lymphocyte - immunology ; Escherichia coli ; Fundamental and applied biological sciences. Psychology ; Fundamental immunology ; Glycoproteins - genetics ; Glycoproteins - immunology ; HYPERSENSITIVITY ; IMMUNOBLOTTING ; Immunoblotting - methods ; major house dust mite allergen ; MANKIND ; Mice ; Mice, Inbred BALB C ; Mites ; Molecular immunology ; MONOCLONAL ANTIBODIES ; Mutagenesis ; PEST MITES ; site-directed mutagenesis</subject><ispartof>Bioscience, biotechnology, and biochemistry, 2001-03, Vol.65 (3), p.694-697</ispartof><rights>2001 by Japan Society for Bioscience, Biotechnology, and Agrochemistry 2001</rights><rights>2001 INIST-CNRS</rights><rights>Copyright Japan Science and Technology Agency 2001</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c524t-79cdf17da791305a00f50c81dbdb71e05b74b02a180a11d5de16dd03970303a3</citedby><cites>FETCH-LOGICAL-c524t-79cdf17da791305a00f50c81dbdb71e05b74b02a180a11d5de16dd03970303a3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,778,782,27911,27912</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=1132347$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/11330693$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Takai, T. (Asahi Breweries Co. Ltd., Moriya, Ibaraki (Japan))</creatorcontrib><creatorcontrib>Akagawa Chihara, M</creatorcontrib><creatorcontrib>Yokota, T</creatorcontrib><creatorcontrib>Okumura, Y</creatorcontrib><title>Reactivities of mutants of a major house dust mite allergen Der f 2 to mouse anti-Der f 2 monoclonal antibodies analyzed by immunoblotting</title><title>Bioscience, biotechnology, and biochemistry</title><addtitle>Biosci Biotechnol Biochem</addtitle><description>A total of sixteen recombinant variants of a major house dust mite allergen Der f 2, the wild-type Der f 2, six cysteine mutants, six proline mutants, and three lysine mutants, were expressed in Escherichia coli. The cells were solubilized and run on SDS-PAGE under reducing conditions. 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Psychology</subject><subject>Fundamental immunology</subject><subject>Glycoproteins - genetics</subject><subject>Glycoproteins - immunology</subject><subject>HYPERSENSITIVITY</subject><subject>IMMUNOBLOTTING</subject><subject>Immunoblotting - methods</subject><subject>major house dust mite allergen</subject><subject>MANKIND</subject><subject>Mice</subject><subject>Mice, Inbred BALB C</subject><subject>Mites</subject><subject>Molecular immunology</subject><subject>MONOCLONAL ANTIBODIES</subject><subject>Mutagenesis</subject><subject>PEST MITES</subject><subject>site-directed mutagenesis</subject><issn>0916-8451</issn><issn>1347-6947</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2001</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNpt0cuKFDEUBuAgijOObtwrAcWFUO1JJVXpWg7jnQFFZh9OKqk2TVIZk5TSPoJPbaa7B0VcJTl8J7efkMcMVqyV7JXWetV3q34Qd8gp40I2dSrvklMYWN-sRcdOyIOctwC10LH75IQxzqEf-Cn59cXiWNx3V5zNNE40LAXnsp8iDbiNiX6NS7bULLnQ4Iql6L1NGzvT1zbRiba0RBr2pna65rYa4hxHH2f0-7qO5uYIrOvdT2uo3lEXwjJH7WMpbt48JPcm9Nk-Oo5n5Ortm6uL983lp3cfLs4vm7FrRWnkMJqJSYNyYBw6BJg6GNfMaKMls9BpKTS0yNaAjJnOWNYbA3yQwIEjPyMvDttep_htsbmo4PJovcfZ1kcoJgeovwoVPvsHbuOS6vWrEWIQXEouqnp5UGOKOSc7qevkAqadYqBu4lE1HtV3qmZS8dPjlosO1vyhxzwqeH4EmEf0U8J5dPlv19Z8K-sOzM1TTAF_xOSNKrjzMd328P-e_-TQN2FUuEmVffzcAjAAAbDmvwF9FLLA</recordid><startdate>20010301</startdate><enddate>20010301</enddate><creator>Takai, T. 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Ltd., Moriya, Ibaraki (Japan)) ; Akagawa Chihara, M ; Yokota, T ; Okumura, Y</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c524t-79cdf17da791305a00f50c81dbdb71e05b74b02a180a11d5de16dd03970303a3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2001</creationdate><topic>ALLERGENS</topic><topic>Allergens - genetics</topic><topic>Allergens - immunology</topic><topic>Animals</topic><topic>Antibodies, Monoclonal - immunology</topic><topic>Antigenic determinants, haptens, artificial antigens</topic><topic>Antigens</topic><topic>Antigens, Dermatophagoides</topic><topic>Biological and medical sciences</topic><topic>Der f 2</topic><topic>epitope</topic><topic>Epitope Mapping</topic><topic>Epitopes, B-Lymphocyte - immunology</topic><topic>Escherichia coli</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Fundamental immunology</topic><topic>Glycoproteins - genetics</topic><topic>Glycoproteins - immunology</topic><topic>HYPERSENSITIVITY</topic><topic>IMMUNOBLOTTING</topic><topic>Immunoblotting - methods</topic><topic>major house dust mite allergen</topic><topic>MANKIND</topic><topic>Mice</topic><topic>Mice, Inbred BALB C</topic><topic>Mites</topic><topic>Molecular immunology</topic><topic>MONOCLONAL ANTIBODIES</topic><topic>Mutagenesis</topic><topic>PEST MITES</topic><topic>site-directed mutagenesis</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Takai, T. (Asahi Breweries Co. 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Epitopes for five mouse anti-Der f 2 monoclonal antibodies, 1B2, 7C10, 13A4, 15E11, and 18G8, to the recombinant Der f 2 variants were characterized by immunoblot analysis.</abstract><cop>Tokyo</cop><pub>Japan Society for Bioscience, Biotechnology, and Agrochemistry</pub><pmid>11330693</pmid><doi>10.1271/bbb.65.694</doi><tpages>4</tpages><oa>free_for_read</oa></addata></record> |
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| ispartof | Bioscience, biotechnology, and biochemistry, 2001-03, Vol.65 (3), p.694-697 |
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| source | J-STAGE Free; MEDLINE; Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals; Oxford University Press Journals All Titles (1996-Current); Open Access Titles of Japan; Free Full-Text Journals in Chemistry |
| subjects | ALLERGENS Allergens - genetics Allergens - immunology Animals Antibodies, Monoclonal - immunology Antigenic determinants, haptens, artificial antigens Antigens Antigens, Dermatophagoides Biological and medical sciences Der f 2 epitope Epitope Mapping Epitopes, B-Lymphocyte - immunology Escherichia coli Fundamental and applied biological sciences. Psychology Fundamental immunology Glycoproteins - genetics Glycoproteins - immunology HYPERSENSITIVITY IMMUNOBLOTTING Immunoblotting - methods major house dust mite allergen MANKIND Mice Mice, Inbred BALB C Mites Molecular immunology MONOCLONAL ANTIBODIES Mutagenesis PEST MITES site-directed mutagenesis |
| title | Reactivities of mutants of a major house dust mite allergen Der f 2 to mouse anti-Der f 2 monoclonal antibodies analyzed by immunoblotting |
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