Gamma-glutamyl transfer reactions by glutaminase from Pseudomonas nitroreducens IFO 12694 and their application for the syntheses of theanine and gamma-glutamylmethylamide
In a mixture containing gamma-glutamyl donor (donor) and gamma glutamyl acceptor (acceptor), the glutaminase of Pseudomonas nitroreducens IFO 12694 simultaneously catalyzed a gamma-glutamyl transfer reaction and hydrolysis of the donor. The variation of the activities responding to the concentration...
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| Veröffentlicht in: | Bioscience, biotechnology, and biochemistry biotechnology, and biochemistry, 1998, Vol.62 (7), p.1279-1283 |
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| container_title | Bioscience, biotechnology, and biochemistry |
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| creator | Tachiki, T. (Ritsumeikan Univ., Kyoto (Japan). Faculty of Science and Engineering) Yamada, T Mizuno, K Ueda, M Shiode, J Fukami, H |
| description | In a mixture containing gamma-glutamyl donor (donor) and gamma glutamyl acceptor (acceptor), the glutaminase of Pseudomonas nitroreducens IFO 12694 simultaneously catalyzed a gamma-glutamyl transfer reaction and hydrolysis of the donor. The variation of the activities responding to the concentration of glutathione and glycylglycine indicated that the enzyme might be classified in a group of glutaminases that shows hydrolysis prior to transfer reaction. On the other hand, the results with glutamine and ethylamine or methylamine indicated that the enzyme was active in the transfer reaction with suppressed hydrolysis of glutamine, and suggested the possibility of using the reaction for producing gamma-glutamylethylamide (theanine) or gamma-glutamylethylamide (gamma-GMA). In fact, in a mixture containing high concentrations of substrates (0.7 M glutamine, 1.5 M ethylamine or methylamine) and 0.5 unit/ml glutaminase (borate buffer pH 11), 270 mM (47 g/L theanine or 250 mM (38 g/L) gamma-GMA was formed in 7 h of incubation at 30 degrees C |
| doi_str_mv | 10.1271/bbb.62.1279 |
| format | Article |
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(Ritsumeikan Univ., Kyoto (Japan). Faculty of Science and Engineering) ; Yamada, T ; Mizuno, K ; Ueda, M ; Shiode, J ; Fukami, H</creator><creatorcontrib>Tachiki, T. (Ritsumeikan Univ., Kyoto (Japan). Faculty of Science and Engineering) ; Yamada, T ; Mizuno, K ; Ueda, M ; Shiode, J ; Fukami, H</creatorcontrib><description>In a mixture containing gamma-glutamyl donor (donor) and gamma glutamyl acceptor (acceptor), the glutaminase of Pseudomonas nitroreducens IFO 12694 simultaneously catalyzed a gamma-glutamyl transfer reaction and hydrolysis of the donor. The variation of the activities responding to the concentration of glutathione and glycylglycine indicated that the enzyme might be classified in a group of glutaminases that shows hydrolysis prior to transfer reaction. On the other hand, the results with glutamine and ethylamine or methylamine indicated that the enzyme was active in the transfer reaction with suppressed hydrolysis of glutamine, and suggested the possibility of using the reaction for producing gamma-glutamylethylamide (theanine) or gamma-glutamylethylamide (gamma-GMA). In fact, in a mixture containing high concentrations of substrates (0.7 M glutamine, 1.5 M ethylamine or methylamine) and 0.5 unit/ml glutaminase (borate buffer pH 11), 270 mM (47 g/L theanine or 250 mM (38 g/L) gamma-GMA was formed in 7 h of incubation at 30 degrees C</description><identifier>ISSN: 0916-8451</identifier><identifier>EISSN: 1347-6947</identifier><identifier>DOI: 10.1271/bbb.62.1279</identifier><identifier>PMID: 27396994</identifier><language>eng</language><publisher>Tokyo: Japan Society for Bioscience, Biotechnology, and Agrochemistry</publisher><subject>AMIDAS ; AMIDE ; AMIDES ; Biological and medical sciences ; Biology of microorganisms of confirmed or potential industrial interest ; BIOSINTESIS ; BIOSYNTHESE ; BIOSYNTHESIS ; Biotechnology ; Fundamental and applied biological sciences. Psychology ; glutaminase ; HIDROLASAS ; HYDROLASE ; HYDROLASES ; Mission oriented research ; Physiology and metabolism ; PSEUDOMONAS ; Pseudomonas nitroreducens ; theanine ; γ-glutamyl transfer reaction ; γ-glutamylmethylamide</subject><ispartof>Bioscience, biotechnology, and biochemistry, 1998, Vol.62 (7), p.1279-1283</ispartof><rights>1998 by Japan Society for Bioscience, Biotechnology, and Agrochemistry 1998</rights><rights>1998 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c300t-21bc078c140fa332dd6d76d631053bef5b02dd1b13cc01798afbbda3510281803</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,776,780,4010,27902,27903,27904</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=2415977$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/27396994$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Tachiki, T. (Ritsumeikan Univ., Kyoto (Japan). Faculty of Science and Engineering)</creatorcontrib><creatorcontrib>Yamada, T</creatorcontrib><creatorcontrib>Mizuno, K</creatorcontrib><creatorcontrib>Ueda, M</creatorcontrib><creatorcontrib>Shiode, J</creatorcontrib><creatorcontrib>Fukami, H</creatorcontrib><title>Gamma-glutamyl transfer reactions by glutaminase from Pseudomonas nitroreducens IFO 12694 and their application for the syntheses of theanine and gamma-glutamylmethylamide</title><title>Bioscience, biotechnology, and biochemistry</title><addtitle>Biosci Biotechnol Biochem</addtitle><description>In a mixture containing gamma-glutamyl donor (donor) and gamma glutamyl acceptor (acceptor), the glutaminase of Pseudomonas nitroreducens IFO 12694 simultaneously catalyzed a gamma-glutamyl transfer reaction and hydrolysis of the donor. The variation of the activities responding to the concentration of glutathione and glycylglycine indicated that the enzyme might be classified in a group of glutaminases that shows hydrolysis prior to transfer reaction. On the other hand, the results with glutamine and ethylamine or methylamine indicated that the enzyme was active in the transfer reaction with suppressed hydrolysis of glutamine, and suggested the possibility of using the reaction for producing gamma-glutamylethylamide (theanine) or gamma-glutamylethylamide (gamma-GMA). In fact, in a mixture containing high concentrations of substrates (0.7 M glutamine, 1.5 M ethylamine or methylamine) and 0.5 unit/ml glutaminase (borate buffer pH 11), 270 mM (47 g/L theanine or 250 mM (38 g/L) gamma-GMA was formed in 7 h of incubation at 30 degrees C</description><subject>AMIDAS</subject><subject>AMIDE</subject><subject>AMIDES</subject><subject>Biological and medical sciences</subject><subject>Biology of microorganisms of confirmed or potential industrial interest</subject><subject>BIOSINTESIS</subject><subject>BIOSYNTHESE</subject><subject>BIOSYNTHESIS</subject><subject>Biotechnology</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>glutaminase</subject><subject>HIDROLASAS</subject><subject>HYDROLASE</subject><subject>HYDROLASES</subject><subject>Mission oriented research</subject><subject>Physiology and metabolism</subject><subject>PSEUDOMONAS</subject><subject>Pseudomonas nitroreducens</subject><subject>theanine</subject><subject>γ-glutamyl transfer reaction</subject><subject>γ-glutamylmethylamide</subject><issn>0916-8451</issn><issn>1347-6947</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1998</creationdate><recordtype>article</recordtype><recordid>eNptkk1rFTEUhoMo9lpduVaycCHI1GQyX1lKaWul0C50PZx83UbycU1mkPlN_slmnFtFcHUOb55z8pI3CL2m5IzWPf0ohDjr6rXnT9COsqavOt70T9GOcNpVQ9PSE_Qi5--EFKGlz9FJ3TPecd7s0K8r8B6qvZsn8IvDU4KQjU44aZCTjSFjseDt2AbIGpsUPb7LelbRx6LgYKcUk1az1IW-vrzFtC4GMASFp3ttE4bDwVkJ6zpsYlpVnJdQStYZR7MKEGzQv2f2_zjyerpfXLlc6ZfomQGX9atjPUXfLi--nn-ubm6vrs8_3VSSETJVNRWS9IOkDTHAWK1Up_pOdYySlgltWkGKRgVlUhLa8wGMEApYS0k90IGwU_R-23tI8ces8zR6m6V2DoKOcx5XpmlZW7cF_bChMsWckzbjIVkPaRkpGdd0xpLO2NVrzwv99rh4Fl6rP-xjHAV4dwQgS3CmhCFt_ss1tOV9X7Buw2woz-nhZ0xOjRMsLqbHGfZ_A2-2QQNxhH0q3Jc7yjlfv0bfsAcEjLZY</recordid><startdate>1998</startdate><enddate>1998</enddate><creator>Tachiki, T. (Ritsumeikan Univ., Kyoto (Japan). Faculty of Science and Engineering)</creator><creator>Yamada, T</creator><creator>Mizuno, K</creator><creator>Ueda, M</creator><creator>Shiode, J</creator><creator>Fukami, H</creator><general>Japan Society for Bioscience, Biotechnology, and Agrochemistry</general><general>Japan Society for Bioscience Biotechnology and Agrochemistry</general><scope>FBQ</scope><scope>IQODW</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>1998</creationdate><title>Gamma-glutamyl transfer reactions by glutaminase from Pseudomonas nitroreducens IFO 12694 and their application for the syntheses of theanine and gamma-glutamylmethylamide</title><author>Tachiki, T. (Ritsumeikan Univ., Kyoto (Japan). Faculty of Science and Engineering) ; Yamada, T ; Mizuno, K ; Ueda, M ; Shiode, J ; Fukami, H</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c300t-21bc078c140fa332dd6d76d631053bef5b02dd1b13cc01798afbbda3510281803</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1998</creationdate><topic>AMIDAS</topic><topic>AMIDE</topic><topic>AMIDES</topic><topic>Biological and medical sciences</topic><topic>Biology of microorganisms of confirmed or potential industrial interest</topic><topic>BIOSINTESIS</topic><topic>BIOSYNTHESE</topic><topic>BIOSYNTHESIS</topic><topic>Biotechnology</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>glutaminase</topic><topic>HIDROLASAS</topic><topic>HYDROLASE</topic><topic>HYDROLASES</topic><topic>Mission oriented research</topic><topic>Physiology and metabolism</topic><topic>PSEUDOMONAS</topic><topic>Pseudomonas nitroreducens</topic><topic>theanine</topic><topic>γ-glutamyl transfer reaction</topic><topic>γ-glutamylmethylamide</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Tachiki, T. (Ritsumeikan Univ., Kyoto (Japan). Faculty of Science and Engineering)</creatorcontrib><creatorcontrib>Yamada, T</creatorcontrib><creatorcontrib>Mizuno, K</creatorcontrib><creatorcontrib>Ueda, M</creatorcontrib><creatorcontrib>Shiode, J</creatorcontrib><creatorcontrib>Fukami, H</creatorcontrib><collection>AGRIS</collection><collection>Pascal-Francis</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Bioscience, biotechnology, and biochemistry</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Tachiki, T. (Ritsumeikan Univ., Kyoto (Japan). Faculty of Science and Engineering)</au><au>Yamada, T</au><au>Mizuno, K</au><au>Ueda, M</au><au>Shiode, J</au><au>Fukami, H</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Gamma-glutamyl transfer reactions by glutaminase from Pseudomonas nitroreducens IFO 12694 and their application for the syntheses of theanine and gamma-glutamylmethylamide</atitle><jtitle>Bioscience, biotechnology, and biochemistry</jtitle><addtitle>Biosci Biotechnol Biochem</addtitle><date>1998</date><risdate>1998</risdate><volume>62</volume><issue>7</issue><spage>1279</spage><epage>1283</epage><pages>1279-1283</pages><issn>0916-8451</issn><eissn>1347-6947</eissn><abstract>In a mixture containing gamma-glutamyl donor (donor) and gamma glutamyl acceptor (acceptor), the glutaminase of Pseudomonas nitroreducens IFO 12694 simultaneously catalyzed a gamma-glutamyl transfer reaction and hydrolysis of the donor. The variation of the activities responding to the concentration of glutathione and glycylglycine indicated that the enzyme might be classified in a group of glutaminases that shows hydrolysis prior to transfer reaction. On the other hand, the results with glutamine and ethylamine or methylamine indicated that the enzyme was active in the transfer reaction with suppressed hydrolysis of glutamine, and suggested the possibility of using the reaction for producing gamma-glutamylethylamide (theanine) or gamma-glutamylethylamide (gamma-GMA). In fact, in a mixture containing high concentrations of substrates (0.7 M glutamine, 1.5 M ethylamine or methylamine) and 0.5 unit/ml glutaminase (borate buffer pH 11), 270 mM (47 g/L theanine or 250 mM (38 g/L) gamma-GMA was formed in 7 h of incubation at 30 degrees C</abstract><cop>Tokyo</cop><pub>Japan Society for Bioscience, Biotechnology, and Agrochemistry</pub><pmid>27396994</pmid><doi>10.1271/bbb.62.1279</doi><tpages>5</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0916-8451 |
| ispartof | Bioscience, biotechnology, and biochemistry, 1998, Vol.62 (7), p.1279-1283 |
| issn | 0916-8451 1347-6947 |
| language | eng |
| recordid | cdi_crossref_primary_10_1271_bbb_62_1279 |
| source | Oxford University Press Journals All Titles (1996-Current); J-STAGE (Japan Science & Technology Information Aggregator, Electronic) Freely Available Titles - Japanese; Open Access Titles of Japan; Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals; Free Full-Text Journals in Chemistry |
| subjects | AMIDAS AMIDE AMIDES Biological and medical sciences Biology of microorganisms of confirmed or potential industrial interest BIOSINTESIS BIOSYNTHESE BIOSYNTHESIS Biotechnology Fundamental and applied biological sciences. Psychology glutaminase HIDROLASAS HYDROLASE HYDROLASES Mission oriented research Physiology and metabolism PSEUDOMONAS Pseudomonas nitroreducens theanine γ-glutamyl transfer reaction γ-glutamylmethylamide |
| title | Gamma-glutamyl transfer reactions by glutaminase from Pseudomonas nitroreducens IFO 12694 and their application for the syntheses of theanine and gamma-glutamylmethylamide |
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