An Alanine Racemase Gene as a New Index for Detecting Escherichia coli in Foods

A gene of alanine racemase, a typical prokaryotic enzyme, was evaluated as a new index for detecting Escherichia coli in foods. An alanine racemase gene fragment containing a non-conserved sequence of the gene was amplified from genomic DNA of E. coli by a polymerase chain reaction, and then labeled...

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Veröffentlicht in:	Bioscience, biotechnology, and biochemistry biotechnology, and biochemistry, 1996, Vol.60 (11), p.1799-1804
Hauptverfasser:	Yokoigawa, Kumio, Tomioka, Rikako, Okubo, Yoko, Kawai, Hiroyasu
Format:	Artikel
Sprache:	eng
Schlagworte:	Alanine Racemase - genetics alanine racemase: Escherichia coli Amino Acid Sequence Base Sequence Biological and medical sciences Culture Media detection Digoxigenin Electrophoresis, Agar Gel Escherichia coli Escherichia coli - chemistry Escherichia coli - enzymology Escherichia coli - genetics Escherichia coli O157 - enzymology Escherichia coli O157 - genetics Food Contamination - analysis Food industries Food microbiology Fundamental and applied biological sciences. Psychology Hybridization, Genetic Molecular Sequence Data Oligonucleotide Probes Polymerase Chain Reaction
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