A Novel Method for Breeding Polyploid Cells by Heat-induced Endomitotic Diploidization in Saccharomyces cerevisiae
Endomitotic diploidization occurred as a result of heat treatment of spores during germination of the industrial yeast S. cerevisiae. Polyploid cells were obtained in both heterothallic and homothallic strains. In the heterothallic strains, the resultant diploid cells, with the ability to mate, were...
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| Veröffentlicht in: | Bioscience, biotechnology, and biochemistry biotechnology, and biochemistry, 1993, Vol.57 (12), p.2063-2066 |
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| container_end_page | 2066 |
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| container_issue | 12 |
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| container_title | Bioscience, biotechnology, and biochemistry |
| container_volume | 57 |
| creator | Tani, Yoshinobu Tomohiro, Yukio Miyata, Akira Kurokui, Takamasa Hayashida, Shinsaku |
| description | Endomitotic diploidization occurred as a result of heat treatment of spores during germination of the industrial yeast S. cerevisiae. Polyploid cells were obtained in both heterothallic and homothallic strains. In the heterothallic strains, the resultant diploid cells, with the ability to mate, were used to construct tetraploids. In the homothallic strains, tetraploid cells were obtained directly after a mating-type switch. In each strain, the highest frequency (approximately 10% of viable cells) of diploidization was obtained when spores were cultured for 30 min in liquid medium to initiate germination followed by subsequent heat treatment at 55°C for 10min. |
| doi_str_mv | 10.1271/bbb.57.2063 |
| format | Article |
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Polyploid cells were obtained in both heterothallic and homothallic strains. In the heterothallic strains, the resultant diploid cells, with the ability to mate, were used to construct tetraploids. In the homothallic strains, tetraploid cells were obtained directly after a mating-type switch. In each strain, the highest frequency (approximately 10% of viable cells) of diploidization was obtained when spores were cultured for 30 min in liquid medium to initiate germination followed by subsequent heat treatment at 55°C for 10min.</description><identifier>ISSN: 0916-8451</identifier><identifier>EISSN: 1347-6947</identifier><identifier>DOI: 10.1271/bbb.57.2063</identifier><language>eng</language><publisher>Tokyo: Taylor & Francis</publisher><subject>Biological and medical sciences ; Cytogenetics ; Fundamental and applied biological sciences. Psychology ; Genetics of eukaryotes. 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| ispartof | Bioscience, biotechnology, and biochemistry, 1993, Vol.57 (12), p.2063-2066 |
| issn | 0916-8451 1347-6947 |
| language | eng |
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| source | J-STAGE Free; Freely Accessible Japanese Titles; EZB-FREE-00999 freely available EZB journals; Free Full-Text Journals in Chemistry |
| subjects | Biological and medical sciences Cytogenetics Fundamental and applied biological sciences. Psychology Genetics of eukaryotes. Biological and molecular evolution Saccharomyces cerevisiae Thallophyta, bryophyta Vegetals and fungi |
| title | A Novel Method for Breeding Polyploid Cells by Heat-induced Endomitotic Diploidization in Saccharomyces cerevisiae |
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