Purification and characterization of membrane-bound 3-dehydroshikimate dehydratase from Gluconobacter oxydans IFO 3244, a new enzyme catalyzing extracellular protocatechuate formation
3-Dehydroshikimate dehydratase (DSD) is the first known enzyme catalyzing aromatization from 3-dehydroshikimate (DSA) to protocatechuate (PCA). Differently from cytosolic DSD (sDSD), a membrane-bound 3-dehydroshikimate dehydratase (mDSD) was found for the first time in the membrane fraction of Gluco...
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| Veröffentlicht in: | Bioscience, biotechnology, and biochemistry biotechnology, and biochemistry, 2010, Vol.74 (5), p.1084-1088 |
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| creator | Shinagawa, E., Ube National Coll. of Technology, Yamaguchi (Japan) Adachi, O Ano, Y Yakushi, T Matsushita, K |
| description | 3-Dehydroshikimate dehydratase (DSD) is the first known enzyme catalyzing aromatization from 3-dehydroshikimate (DSA) to protocatechuate (PCA). Differently from cytosolic DSD (sDSD), a membrane-bound 3-dehydroshikimate dehydratase (mDSD) was found for the first time in the membrane fraction of Gluconobacter oxydans IFO 3244, and DSA was confirmed to be the direct precursor of PCA. In contrast to weak and instable sDSD, the abundance of mDSD in the membrane fraction suggested the metabolic significance of mDSD as the initial step in aromatization. mDSD was solubilized only by a detergent and was readily purified to high homogeneity. Its molecular weight was estimated to be 76,000. Purified mDSD showed a sole peak at 280 nm in the absorption spectrum and no critical cofactor requirements. The Km of DSA was measured at 0.5 mM, and the optimum pH was observed at pH 6-8. mDSD appeared to react only with DSA, and was inert to other compounds, such as 3-dehydroquinate, quinate, and shikimate. |
| doi_str_mv | 10.1271/bbb.100043 |
| format | Article |
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Differently from cytosolic DSD (sDSD), a membrane-bound 3-dehydroshikimate dehydratase (mDSD) was found for the first time in the membrane fraction of Gluconobacter oxydans IFO 3244, and DSA was confirmed to be the direct precursor of PCA. In contrast to weak and instable sDSD, the abundance of mDSD in the membrane fraction suggested the metabolic significance of mDSD as the initial step in aromatization. mDSD was solubilized only by a detergent and was readily purified to high homogeneity. Its molecular weight was estimated to be 76,000. Purified mDSD showed a sole peak at 280 nm in the absorption spectrum and no critical cofactor requirements. The Km of DSA was measured at 0.5 mM, and the optimum pH was observed at pH 6-8. mDSD appeared to react only with DSA, and was inert to other compounds, such as 3-dehydroquinate, quinate, and shikimate.</description><identifier>ISSN: 0916-8451</identifier><identifier>EISSN: 1347-6947</identifier><identifier>DOI: 10.1271/bbb.100043</identifier><identifier>PMID: 20460715</identifier><language>eng</language><publisher>Tokyo: Japan Society for Bioscience, Biotechnology, and Agrochemistry</publisher><subject>3-dehydroshikimate dehydratase ; Absorption ; Acetates - metabolism ; acetic acid bacteria ; ACIDE ORGANIQUE ; ACIDOS ORGANICOS ; AROMATIC COMPOUNDS ; Biocatalysis ; Biological and medical sciences ; BIOSINTESIS ; BIOSYNTHESE ; BIOSYNTHESIS ; Biotechnology ; CATECHIN ; CATECHINE ; CATEQUINA ; Cell Membrane - metabolism ; CHEMICOPHYSICAL PROPERTIES ; COMPOSE AROMATIQUE ; COMPUESTOS AROMATICOS ; enzymatic aromatization ; Extracellular Space - metabolism ; FERMENTACION ; FERMENTATION ; Fundamental and applied biological sciences. Psychology ; Gluconobacter oxydans ; Gluconobacter oxydans - cytology ; Gluconobacter oxydans - enzymology ; http://www.fao.org/aos/agrovoc#c_1521 ; http://www.fao.org/aos/agrovoc#c_28457 ; http://www.fao.org/aos/agrovoc#c_2855 ; http://www.fao.org/aos/agrovoc#c_33963 ; http://www.fao.org/aos/agrovoc#c_4473 ; http://www.fao.org/aos/agrovoc#c_5383 ; http://www.fao.org/aos/agrovoc#c_622 ; http://www.fao.org/aos/agrovoc#c_6303 ; http://www.fao.org/aos/agrovoc#c_6377 ; http://www.fao.org/aos/agrovoc#c_928 ; Hydro-Lyases - chemistry ; Hydro-Lyases - isolation & purification ; Hydro-Lyases - metabolism ; Hydrogen-Ion Concentration ; Hydroxybenzoates - metabolism ; LIASAS ; LYASE ; LYASES ; MEMBRANA ; MEMBRANE ; MEMBRANES ; Methods. Procedures. Technologies ; Microbial engineering. Fermentation and microbial culture technology ; ORGANIC ACIDS ; oxidative fermentation ; PROPIEDADES FISICOQUIMICAS ; PROPRIETE PHYSICOCHIMIQUE ; PSEUDOMONACEAE ; PURIFICACION ; PURIFICATION ; shikimate pathway ; Solubility</subject><ispartof>Bioscience, biotechnology, and biochemistry, 2010, Vol.74 (5), p.1084-1088</ispartof><rights>2010 by Japan Society for Bioscience, Biotechnology, and Agrochemistry 2010</rights><rights>2015 INIST-CNRS</rights><rights>Copyright Japan Science and Technology Agency 2010</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c571t-ff4a6ef2f4ad2f111465b18516c5851192d01c8afe922d206bb2cdc93b2ed4513</citedby><cites>FETCH-LOGICAL-c571t-ff4a6ef2f4ad2f111465b18516c5851192d01c8afe922d206bb2cdc93b2ed4513</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,776,780,4009,27902,27903,27904</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=23147162$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/20460715$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Shinagawa, E., Ube National Coll. of Technology, Yamaguchi (Japan)</creatorcontrib><creatorcontrib>Adachi, O</creatorcontrib><creatorcontrib>Ano, Y</creatorcontrib><creatorcontrib>Yakushi, T</creatorcontrib><creatorcontrib>Matsushita, K</creatorcontrib><title>Purification and characterization of membrane-bound 3-dehydroshikimate dehydratase from Gluconobacter oxydans IFO 3244, a new enzyme catalyzing extracellular protocatechuate formation</title><title>Bioscience, biotechnology, and biochemistry</title><addtitle>Biosci Biotechnol Biochem</addtitle><description>3-Dehydroshikimate dehydratase (DSD) is the first known enzyme catalyzing aromatization from 3-dehydroshikimate (DSA) to protocatechuate (PCA). Differently from cytosolic DSD (sDSD), a membrane-bound 3-dehydroshikimate dehydratase (mDSD) was found for the first time in the membrane fraction of Gluconobacter oxydans IFO 3244, and DSA was confirmed to be the direct precursor of PCA. In contrast to weak and instable sDSD, the abundance of mDSD in the membrane fraction suggested the metabolic significance of mDSD as the initial step in aromatization. mDSD was solubilized only by a detergent and was readily purified to high homogeneity. Its molecular weight was estimated to be 76,000. Purified mDSD showed a sole peak at 280 nm in the absorption spectrum and no critical cofactor requirements. The Km of DSA was measured at 0.5 mM, and the optimum pH was observed at pH 6-8. mDSD appeared to react only with DSA, and was inert to other compounds, such as 3-dehydroquinate, quinate, and shikimate.</description><subject>3-dehydroshikimate dehydratase</subject><subject>Absorption</subject><subject>Acetates - metabolism</subject><subject>acetic acid bacteria</subject><subject>ACIDE ORGANIQUE</subject><subject>ACIDOS ORGANICOS</subject><subject>AROMATIC COMPOUNDS</subject><subject>Biocatalysis</subject><subject>Biological and medical sciences</subject><subject>BIOSINTESIS</subject><subject>BIOSYNTHESE</subject><subject>BIOSYNTHESIS</subject><subject>Biotechnology</subject><subject>CATECHIN</subject><subject>CATECHINE</subject><subject>CATEQUINA</subject><subject>Cell Membrane - metabolism</subject><subject>CHEMICOPHYSICAL PROPERTIES</subject><subject>COMPOSE AROMATIQUE</subject><subject>COMPUESTOS AROMATICOS</subject><subject>enzymatic aromatization</subject><subject>Extracellular Space - metabolism</subject><subject>FERMENTACION</subject><subject>FERMENTATION</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Gluconobacter oxydans</subject><subject>Gluconobacter oxydans - cytology</subject><subject>Gluconobacter oxydans - enzymology</subject><subject>http://www.fao.org/aos/agrovoc#c_1521</subject><subject>http://www.fao.org/aos/agrovoc#c_28457</subject><subject>http://www.fao.org/aos/agrovoc#c_2855</subject><subject>http://www.fao.org/aos/agrovoc#c_33963</subject><subject>http://www.fao.org/aos/agrovoc#c_4473</subject><subject>http://www.fao.org/aos/agrovoc#c_5383</subject><subject>http://www.fao.org/aos/agrovoc#c_622</subject><subject>http://www.fao.org/aos/agrovoc#c_6303</subject><subject>http://www.fao.org/aos/agrovoc#c_6377</subject><subject>http://www.fao.org/aos/agrovoc#c_928</subject><subject>Hydro-Lyases - chemistry</subject><subject>Hydro-Lyases - isolation & purification</subject><subject>Hydro-Lyases - metabolism</subject><subject>Hydrogen-Ion Concentration</subject><subject>Hydroxybenzoates - metabolism</subject><subject>LIASAS</subject><subject>LYASE</subject><subject>LYASES</subject><subject>MEMBRANA</subject><subject>MEMBRANE</subject><subject>MEMBRANES</subject><subject>Methods. Procedures. Technologies</subject><subject>Microbial engineering. Fermentation and microbial culture technology</subject><subject>ORGANIC ACIDS</subject><subject>oxidative fermentation</subject><subject>PROPIEDADES FISICOQUIMICAS</subject><subject>PROPRIETE PHYSICOCHIMIQUE</subject><subject>PSEUDOMONACEAE</subject><subject>PURIFICACION</subject><subject>PURIFICATION</subject><subject>shikimate pathway</subject><subject>Solubility</subject><issn>0916-8451</issn><issn>1347-6947</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2010</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkV1rFDEUhgdR7Fq98V4JiAji1Jwk83UpxdZKob3Q6yGf3dSZSZvM0M7-Mf-eZ3e2CiJIIIFznpyP982yl0CPgFXwUSl1BJRSwR9lK-CiystGVI-zFW2gzGtRwEH2LKVrRBoo4Gl2wKgoaQXFKvt5OUXvvJajDwORgyF6LaPUo41-swSDI73tVZSDzVWYEOG5sevZxJDW_ofv5WjJEpCjTJa4GHpy2k06DEHtSpFwPxs5JHJ2ckE4E-IDkWSwd8QOm7m3BNvLbt744YrY-xHb266bOhnJTQxjwKzV62nbxoXY76Z6nj1xskv2xf49zL6ffP52_CU_vzg9O_50nuuigjF3TsjSOoaPYQ4ARFkoqAsodYE3NMxQ0LV0tmHMMFoqxbTRDVfMGhSOH2bvlro4ye1k09j2Pm3HQzXClNqqrqAUlNf_JzkHWjU1RfLNX-R1mOKAa7QgRFMLwUEg9X6hNOqconXtTUSt49wCbbe-t-h7u_iO8Ot9yUn11vxGH4xG4O0ekEnLzqGb2qc_HHbERRhyxcL5Yaf1XYidaUc5dyE-fOL_HODV8s_J0MqriNjXS0a3WQ54fgGmy9Nc</recordid><startdate>2010</startdate><enddate>2010</enddate><creator>Shinagawa, E., Ube National Coll. of Technology, Yamaguchi (Japan)</creator><creator>Adachi, O</creator><creator>Ano, Y</creator><creator>Yakushi, T</creator><creator>Matsushita, K</creator><general>Japan Society for Bioscience, Biotechnology, and Agrochemistry</general><general>Japan Society for Bioscience Biotechnology and Agrochemistry</general><general>Oxford University Press</general><scope>FBQ</scope><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><scope>7QO</scope><scope>8FD</scope><scope>FR3</scope><scope>P64</scope></search><sort><creationdate>2010</creationdate><title>Purification and characterization of membrane-bound 3-dehydroshikimate dehydratase from Gluconobacter oxydans IFO 3244, a new enzyme catalyzing extracellular protocatechuate formation</title><author>Shinagawa, E., Ube National Coll. of Technology, Yamaguchi (Japan) ; Adachi, O ; Ano, Y ; Yakushi, T ; Matsushita, K</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c571t-ff4a6ef2f4ad2f111465b18516c5851192d01c8afe922d206bb2cdc93b2ed4513</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2010</creationdate><topic>3-dehydroshikimate dehydratase</topic><topic>Absorption</topic><topic>Acetates - metabolism</topic><topic>acetic acid bacteria</topic><topic>ACIDE ORGANIQUE</topic><topic>ACIDOS ORGANICOS</topic><topic>AROMATIC COMPOUNDS</topic><topic>Biocatalysis</topic><topic>Biological and medical sciences</topic><topic>BIOSINTESIS</topic><topic>BIOSYNTHESE</topic><topic>BIOSYNTHESIS</topic><topic>Biotechnology</topic><topic>CATECHIN</topic><topic>CATECHINE</topic><topic>CATEQUINA</topic><topic>Cell Membrane - metabolism</topic><topic>CHEMICOPHYSICAL PROPERTIES</topic><topic>COMPOSE AROMATIQUE</topic><topic>COMPUESTOS AROMATICOS</topic><topic>enzymatic aromatization</topic><topic>Extracellular Space - metabolism</topic><topic>FERMENTACION</topic><topic>FERMENTATION</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Gluconobacter oxydans</topic><topic>Gluconobacter oxydans - cytology</topic><topic>Gluconobacter oxydans - enzymology</topic><topic>http://www.fao.org/aos/agrovoc#c_1521</topic><topic>http://www.fao.org/aos/agrovoc#c_28457</topic><topic>http://www.fao.org/aos/agrovoc#c_2855</topic><topic>http://www.fao.org/aos/agrovoc#c_33963</topic><topic>http://www.fao.org/aos/agrovoc#c_4473</topic><topic>http://www.fao.org/aos/agrovoc#c_5383</topic><topic>http://www.fao.org/aos/agrovoc#c_622</topic><topic>http://www.fao.org/aos/agrovoc#c_6303</topic><topic>http://www.fao.org/aos/agrovoc#c_6377</topic><topic>http://www.fao.org/aos/agrovoc#c_928</topic><topic>Hydro-Lyases - chemistry</topic><topic>Hydro-Lyases - isolation & purification</topic><topic>Hydro-Lyases - metabolism</topic><topic>Hydrogen-Ion Concentration</topic><topic>Hydroxybenzoates - metabolism</topic><topic>LIASAS</topic><topic>LYASE</topic><topic>LYASES</topic><topic>MEMBRANA</topic><topic>MEMBRANE</topic><topic>MEMBRANES</topic><topic>Methods. Procedures. Technologies</topic><topic>Microbial engineering. Fermentation and microbial culture technology</topic><topic>ORGANIC ACIDS</topic><topic>oxidative fermentation</topic><topic>PROPIEDADES FISICOQUIMICAS</topic><topic>PROPRIETE PHYSICOCHIMIQUE</topic><topic>PSEUDOMONACEAE</topic><topic>PURIFICACION</topic><topic>PURIFICATION</topic><topic>shikimate pathway</topic><topic>Solubility</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Shinagawa, E., Ube National Coll. of Technology, Yamaguchi (Japan)</creatorcontrib><creatorcontrib>Adachi, O</creatorcontrib><creatorcontrib>Ano, Y</creatorcontrib><creatorcontrib>Yakushi, T</creatorcontrib><creatorcontrib>Matsushita, K</creatorcontrib><collection>AGRIS</collection><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><collection>Biotechnology Research Abstracts</collection><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><jtitle>Bioscience, biotechnology, and biochemistry</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Shinagawa, E., Ube National Coll. of Technology, Yamaguchi (Japan)</au><au>Adachi, O</au><au>Ano, Y</au><au>Yakushi, T</au><au>Matsushita, K</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Purification and characterization of membrane-bound 3-dehydroshikimate dehydratase from Gluconobacter oxydans IFO 3244, a new enzyme catalyzing extracellular protocatechuate formation</atitle><jtitle>Bioscience, biotechnology, and biochemistry</jtitle><addtitle>Biosci Biotechnol Biochem</addtitle><date>2010</date><risdate>2010</risdate><volume>74</volume><issue>5</issue><spage>1084</spage><epage>1088</epage><pages>1084-1088</pages><issn>0916-8451</issn><eissn>1347-6947</eissn><abstract>3-Dehydroshikimate dehydratase (DSD) is the first known enzyme catalyzing aromatization from 3-dehydroshikimate (DSA) to protocatechuate (PCA). Differently from cytosolic DSD (sDSD), a membrane-bound 3-dehydroshikimate dehydratase (mDSD) was found for the first time in the membrane fraction of Gluconobacter oxydans IFO 3244, and DSA was confirmed to be the direct precursor of PCA. In contrast to weak and instable sDSD, the abundance of mDSD in the membrane fraction suggested the metabolic significance of mDSD as the initial step in aromatization. mDSD was solubilized only by a detergent and was readily purified to high homogeneity. Its molecular weight was estimated to be 76,000. Purified mDSD showed a sole peak at 280 nm in the absorption spectrum and no critical cofactor requirements. The Km of DSA was measured at 0.5 mM, and the optimum pH was observed at pH 6-8. mDSD appeared to react only with DSA, and was inert to other compounds, such as 3-dehydroquinate, quinate, and shikimate.</abstract><cop>Tokyo</cop><pub>Japan Society for Bioscience, Biotechnology, and Agrochemistry</pub><pmid>20460715</pmid><doi>10.1271/bbb.100043</doi><tpages>5</tpages></addata></record> |
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| ispartof | Bioscience, biotechnology, and biochemistry, 2010, Vol.74 (5), p.1084-1088 |
| issn | 0916-8451 1347-6947 |
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| recordid | cdi_crossref_primary_10_1271_bbb_100043 |
| source | J-STAGE Free; MEDLINE; Oxford University Press Journals All Titles (1996-Current); Freely Accessible Japanese Titles; EZB-FREE-00999 freely available EZB journals; Free Full-Text Journals in Chemistry |
| subjects | 3-dehydroshikimate dehydratase Absorption Acetates - metabolism acetic acid bacteria ACIDE ORGANIQUE ACIDOS ORGANICOS AROMATIC COMPOUNDS Biocatalysis Biological and medical sciences BIOSINTESIS BIOSYNTHESE BIOSYNTHESIS Biotechnology CATECHIN CATECHINE CATEQUINA Cell Membrane - metabolism CHEMICOPHYSICAL PROPERTIES COMPOSE AROMATIQUE COMPUESTOS AROMATICOS enzymatic aromatization Extracellular Space - metabolism FERMENTACION FERMENTATION Fundamental and applied biological sciences. Psychology Gluconobacter oxydans Gluconobacter oxydans - cytology Gluconobacter oxydans - enzymology http://www.fao.org/aos/agrovoc#c_1521 http://www.fao.org/aos/agrovoc#c_28457 http://www.fao.org/aos/agrovoc#c_2855 http://www.fao.org/aos/agrovoc#c_33963 http://www.fao.org/aos/agrovoc#c_4473 http://www.fao.org/aos/agrovoc#c_5383 http://www.fao.org/aos/agrovoc#c_622 http://www.fao.org/aos/agrovoc#c_6303 http://www.fao.org/aos/agrovoc#c_6377 http://www.fao.org/aos/agrovoc#c_928 Hydro-Lyases - chemistry Hydro-Lyases - isolation & purification Hydro-Lyases - metabolism Hydrogen-Ion Concentration Hydroxybenzoates - metabolism LIASAS LYASE LYASES MEMBRANA MEMBRANE MEMBRANES Methods. Procedures. Technologies Microbial engineering. Fermentation and microbial culture technology ORGANIC ACIDS oxidative fermentation PROPIEDADES FISICOQUIMICAS PROPRIETE PHYSICOCHIMIQUE PSEUDOMONACEAE PURIFICACION PURIFICATION shikimate pathway Solubility |
| title | Purification and characterization of membrane-bound 3-dehydroshikimate dehydratase from Gluconobacter oxydans IFO 3244, a new enzyme catalyzing extracellular protocatechuate formation |
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