Effect of ß-glucan from Euglena gracilis as an antioxidant on goat semen cryopreservation

Effect of ß-glucan from Euglena gracilis as an antioxidant on goat semen cryopreservation

This study aimed to determine the influence of different doses of β-glucan on post-thaw spermatological parameters, lipid peroxidation, and total antioxidant activity of buck semen. In the non-breeding season, semen was collected from bucks twice weekly. After then, ejaculates were pooled and divide...

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Veröffentlicht in:Journal of the Hellenic Veterinary Medical Society 2024-07, Vol.75 (2), p.7361-7370
Hauptverfasser: Ustuner, B, Soylu, MK, Sengul, E, Karakci, D, Gokce, E, Aktar, A, Ak Sonat, F, Cimen, T, Huraydin, O, Yılmaz, MM, Nur, Z
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container_issue 2
container_start_page 7361
container_title Journal of the Hellenic Veterinary Medical Society
container_volume 75
creator Ustuner, B
Soylu, MK
Sengul, E
Karakci, D
Gokce, E
Aktar, A
Ak Sonat, F
Cimen, T
Huraydin, O
Yılmaz, MM
Nur, Z
description This study aimed to determine the influence of different doses of β-glucan on post-thaw spermatological parameters, lipid peroxidation, and total antioxidant activity of buck semen. In the non-breeding season, semen was collected from bucks twice weekly. After then, ejaculates were pooled and divided into four equal aliquots: β-glucan concentrations of 1 mM (βG1), 2 mM (βG2), and 4 mM (βG4), and a control group without antioxidants. Each sample group was diluted for cryopreservation using a dilution method involving two steps. The experimental groups were then evaluated for several parameters including sperm motility, plasma membrane functional integrity [hypoosmotic swelling test (HOST)], damaged acrosome rate [FITC-Pisum sativum agglutinin (FITC-PSA)], DNA integrity [terminal deoxynucleotidyl transferase-mediated dUTP nick-end labeling (TUNEL)], mitochondrial membrane potential using JC-1, evaluation of lipid peroxidation, and determination of total antioxidant activity. The post-thaw motility and plasma functional integrity of the control group were significantly lower than those values in the βG groups (P < 0.05). Although the numerically greatest acrosome damage was detected in the control group, it was only statistically different from βG1 and βG4 (P
doi_str_mv 10.12681/jhvms.34504
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Although the numerically greatest acrosome damage was detected in the control group, it was only statistically different from βG1 and βG4 (P&lt;0.05). While the DNA fragmentation rate of the control group was higher than βG4 group (P&lt;0.05), it was similar to βG1 and βG2 groups (P&gt;0.05). There was no statistical difference among all the groups regarding low mitochondrial membrane potential, MDA, and TAC rates. 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title Effect of ß-glucan from Euglena gracilis as an antioxidant on goat semen cryopreservation
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