Bovine nuclear transfer using cumulus cells derived from serum-starved and confluent cultures

Bovine cumulus cells after oocyte maturation were cultured under serum-starvation condition or grown to confluence. Their cell size, cell cycle phases, and developmental potential as donors for nuclear transfer (NT) were investigated. The majority of trypsinized cells of both cultures were found wit...

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Veröffentlicht in:	Journal of Reproduction and Development 2000/04/25, Vol.46(2), pp.85-92
Hauptverfasser:	Mohamed Nour, M.S. (Hokkaido Univ., Sapporo (Japan)), Ikeda, K, Takahashi, Y
Format:	Artikel
Sprache:	eng
Schlagworte:	BLOOD SERUM BOVIN Bovine CATTLE CELL CULTURE Cell size CULTIVO DE CELULAS CULTURE DE CELLULE CULTURE MEDIA Cumulus cells GANADO BOVINO GENE TRANSFER MEDIO DE CULTIVO MILIEU DE CULTURE NOYAU CELLULAIRE Nuclear transfer NUCLEO NUCLEUS SERUM SANGUIN SUERO SANGUINEO TRANSFERENCIA DE GENES TRANSFERT DE GENE
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