Bovine nuclear transfer using cumulus cells derived from serum-starved and confluent cultures
Bovine cumulus cells after oocyte maturation were cultured under serum-starvation condition or grown to confluence. Their cell size, cell cycle phases, and developmental potential as donors for nuclear transfer (NT) were investigated. The majority of trypsinized cells of both cultures were found wit...
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| Veröffentlicht in: | Journal of Reproduction and Development 2000/04/25, Vol.46(2), pp.85-92 |
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| container_title | Journal of Reproduction and Development |
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| creator | Mohamed Nour, M.S. (Hokkaido Univ., Sapporo (Japan)) Ikeda, K Takahashi, Y |
| description | Bovine cumulus cells after oocyte maturation were cultured under serum-starvation condition or grown to confluence. Their cell size, cell cycle phases, and developmental potential as donors for nuclear transfer (NT) were investigated. The majority of trypsinized cells of both cultures were found within the medium size range (15-20 μm). Flow cytometric analysis revealed that small- and medium-sized cells had significantly higher percentages of nuclei existing in the G0/G1 phase (95-98%) than large-sized cells (82%) regardless of the culture conditions. Serum-starved cells of different sizes (small, 9-14 μm; medium, 15-20 μm; large, 21-26 μm) had the same fusion rate, cleavage, development to blastocysts, and blastocyst cell number of NT embryos. NT embryos reconstituted from serum-starved and confluent medium-sized cells showed the same developmental rate. These results indicate that the most available medium-sized cells derived from both serum-starvation and confluent cultures can be used as a G0/G1-phase nuclei source. |
| doi_str_mv | 10.1262/jrd.46.85 |
| format | Article |
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(Hokkaido Univ., Sapporo (Japan)) ; Ikeda, K ; Takahashi, Y</creator><creatorcontrib>Mohamed Nour, M.S. (Hokkaido Univ., Sapporo (Japan)) ; Ikeda, K ; Takahashi, Y</creatorcontrib><description>Bovine cumulus cells after oocyte maturation were cultured under serum-starvation condition or grown to confluence. Their cell size, cell cycle phases, and developmental potential as donors for nuclear transfer (NT) were investigated. The majority of trypsinized cells of both cultures were found within the medium size range (15-20 μm). Flow cytometric analysis revealed that small- and medium-sized cells had significantly higher percentages of nuclei existing in the G0/G1 phase (95-98%) than large-sized cells (82%) regardless of the culture conditions. Serum-starved cells of different sizes (small, 9-14 μm; medium, 15-20 μm; large, 21-26 μm) had the same fusion rate, cleavage, development to blastocysts, and blastocyst cell number of NT embryos. NT embryos reconstituted from serum-starved and confluent medium-sized cells showed the same developmental rate. These results indicate that the most available medium-sized cells derived from both serum-starvation and confluent cultures can be used as a G0/G1-phase nuclei source.</description><identifier>ISSN: 0916-8818</identifier><identifier>EISSN: 1348-4400</identifier><identifier>DOI: 10.1262/jrd.46.85</identifier><language>eng</language><publisher>THE SOCIETY FOR REPRODUCTION AND DEVELOPMENT</publisher><subject>BLOOD SERUM ; BOVIN ; Bovine ; CATTLE ; CELL CULTURE ; Cell size ; CULTIVO DE CELULAS ; CULTURE DE CELLULE ; CULTURE MEDIA ; Cumulus cells ; GANADO BOVINO ; GENE TRANSFER ; MEDIO DE CULTIVO ; MILIEU DE CULTURE ; NOYAU CELLULAIRE ; Nuclear transfer ; NUCLEO ; NUCLEUS ; SERUM SANGUIN ; SUERO SANGUINEO ; TRANSFERENCIA DE GENES ; TRANSFERT DE GENE</subject><ispartof>Journal of Reproduction and Development, 2000/04/25, Vol.46(2), pp.85-92</ispartof><rights>2000 Society for Reproduction and Development</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c4025-a124cfd9dd26f33b606f62ed88619844b0964ca7ffa876c34d615ca0366253823</citedby><cites>FETCH-LOGICAL-c4025-a124cfd9dd26f33b606f62ed88619844b0964ca7ffa876c34d615ca0366253823</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,864,1883,4024,27923,27924,27925</link.rule.ids></links><search><creatorcontrib>Mohamed Nour, M.S. (Hokkaido Univ., Sapporo (Japan))</creatorcontrib><creatorcontrib>Ikeda, K</creatorcontrib><creatorcontrib>Takahashi, Y</creatorcontrib><title>Bovine nuclear transfer using cumulus cells derived from serum-starved and confluent cultures</title><title>Journal of Reproduction and Development</title><addtitle>J. Reprod. Dev.</addtitle><description>Bovine cumulus cells after oocyte maturation were cultured under serum-starvation condition or grown to confluence. Their cell size, cell cycle phases, and developmental potential as donors for nuclear transfer (NT) were investigated. The majority of trypsinized cells of both cultures were found within the medium size range (15-20 μm). Flow cytometric analysis revealed that small- and medium-sized cells had significantly higher percentages of nuclei existing in the G0/G1 phase (95-98%) than large-sized cells (82%) regardless of the culture conditions. Serum-starved cells of different sizes (small, 9-14 μm; medium, 15-20 μm; large, 21-26 μm) had the same fusion rate, cleavage, development to blastocysts, and blastocyst cell number of NT embryos. NT embryos reconstituted from serum-starved and confluent medium-sized cells showed the same developmental rate. These results indicate that the most available medium-sized cells derived from both serum-starvation and confluent cultures can be used as a G0/G1-phase nuclei source.</description><subject>BLOOD SERUM</subject><subject>BOVIN</subject><subject>Bovine</subject><subject>CATTLE</subject><subject>CELL CULTURE</subject><subject>Cell size</subject><subject>CULTIVO DE CELULAS</subject><subject>CULTURE DE CELLULE</subject><subject>CULTURE MEDIA</subject><subject>Cumulus cells</subject><subject>GANADO BOVINO</subject><subject>GENE TRANSFER</subject><subject>MEDIO DE CULTIVO</subject><subject>MILIEU DE CULTURE</subject><subject>NOYAU CELLULAIRE</subject><subject>Nuclear transfer</subject><subject>NUCLEO</subject><subject>NUCLEUS</subject><subject>SERUM SANGUIN</subject><subject>SUERO SANGUINEO</subject><subject>TRANSFERENCIA DE GENES</subject><subject>TRANSFERT DE GENE</subject><issn>0916-8818</issn><issn>1348-4400</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2000</creationdate><recordtype>article</recordtype><recordid>eNo9kE1Lw0AQhhdRsFYP_gBhrx5S9yvTzUVQ8ZOCHvQoy3Y_akqayGy24L83Jdo5zMA7z7wMLyHnnM24AHG1Rj9TMNPlAZlwqXShFGOHZMIqDoXWXB-Tk5TWjElRgpqQz9tuW7eBttk1wSLt0bYpBqQ51e2KurzJTU7UhaZJ1Aest8HTiN2GpoB5U6Te4k6yraeua2OTQ9sPZ02fMaRTchRtk8LZ35ySj4f797unYvH6-Hx3syicYqIsLBfKRV95LyBKuQQGEUTwWgOvtFJLVoFydh6j1XNwUnngpbNMAohSaiGn5HL0ddilhCGab6w3Fn8MZ2aXixlyMQqMLgf2emTXw--rsCct9vWQwT8pxqbL_cJ9WTShHQwuRoNoO2NXWCfz8ibYrpQAJn8Bes12wQ</recordid><startdate>2000</startdate><enddate>2000</enddate><creator>Mohamed Nour, M.S. 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(Hokkaido Univ., Sapporo (Japan))</creatorcontrib><creatorcontrib>Ikeda, K</creatorcontrib><creatorcontrib>Takahashi, Y</creatorcontrib><collection>AGRIS</collection><collection>CrossRef</collection><jtitle>Journal of Reproduction and Development</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Mohamed Nour, M.S. (Hokkaido Univ., Sapporo (Japan))</au><au>Ikeda, K</au><au>Takahashi, Y</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Bovine nuclear transfer using cumulus cells derived from serum-starved and confluent cultures</atitle><jtitle>Journal of Reproduction and Development</jtitle><addtitle>J. Reprod. Dev.</addtitle><date>2000</date><risdate>2000</risdate><volume>46</volume><issue>2</issue><spage>85</spage><epage>92</epage><pages>85-92</pages><issn>0916-8818</issn><eissn>1348-4400</eissn><abstract>Bovine cumulus cells after oocyte maturation were cultured under serum-starvation condition or grown to confluence. Their cell size, cell cycle phases, and developmental potential as donors for nuclear transfer (NT) were investigated. The majority of trypsinized cells of both cultures were found within the medium size range (15-20 μm). Flow cytometric analysis revealed that small- and medium-sized cells had significantly higher percentages of nuclei existing in the G0/G1 phase (95-98%) than large-sized cells (82%) regardless of the culture conditions. Serum-starved cells of different sizes (small, 9-14 μm; medium, 15-20 μm; large, 21-26 μm) had the same fusion rate, cleavage, development to blastocysts, and blastocyst cell number of NT embryos. NT embryos reconstituted from serum-starved and confluent medium-sized cells showed the same developmental rate. These results indicate that the most available medium-sized cells derived from both serum-starvation and confluent cultures can be used as a G0/G1-phase nuclei source.</abstract><pub>THE SOCIETY FOR REPRODUCTION AND DEVELOPMENT</pub><doi>10.1262/jrd.46.85</doi><tpages>8</tpages><oa>free_for_read</oa></addata></record> |
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| issn | 0916-8818 1348-4400 |
| language | eng |
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| source | J-STAGE Free; DOAJ Directory of Open Access Journals; EZB-FREE-00999 freely available EZB journals |
| subjects | BLOOD SERUM BOVIN Bovine CATTLE CELL CULTURE Cell size CULTIVO DE CELULAS CULTURE DE CELLULE CULTURE MEDIA Cumulus cells GANADO BOVINO GENE TRANSFER MEDIO DE CULTIVO MILIEU DE CULTURE NOYAU CELLULAIRE Nuclear transfer NUCLEO NUCLEUS SERUM SANGUIN SUERO SANGUINEO TRANSFERENCIA DE GENES TRANSFERT DE GENE |
| title | Bovine nuclear transfer using cumulus cells derived from serum-starved and confluent cultures |
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