Serum-Deprivation Induces Cell Death of Rat Cultured Microglia Accompanied With Expression of Bax Protein

Serum-Deprivation Induces Cell Death of Rat Cultured Microglia Accompanied With Expression of Bax Protein

Expression of Bcl−2 related proteins in rat microglial primary culture was examined.At relative low cell densities, serum deprivation caused cell death and nuclei condensation of cultured microglia.Expression of a pro−apoptotic protein, Bax, but not Bcl−2, was increased by the serum deprivation.SB20...

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Veröffentlicht in:Japanese journal of pharmacology 2000, Vol.83(4), pp.351-354
Hauptverfasser: Koyama, Yutaka, Kimura, Yuji, Yoshioka, Yasuhiro, Wakamatsu, Daisuke, Kozaki, Ryo-hei, Hashimoto, Hitoshi, Matsuda, Toshio, Baba, Akemichi
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Apoptosis
Bax
Microglia
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container_end_page 354
container_issue 4
container_start_page 351
container_title Japanese journal of pharmacology
container_volume 83
creator Koyama, Yutaka
Kimura, Yuji
Yoshioka, Yasuhiro
Wakamatsu, Daisuke
Kozaki, Ryo-hei
Hashimoto, Hitoshi
Matsuda, Toshio
Baba, Akemichi
description Expression of Bcl−2 related proteins in rat microglial primary culture was examined.At relative low cell densities, serum deprivation caused cell death and nuclei condensation of cultured microglia.Expression of a pro−apoptotic protein, Bax, but not Bcl−2, was increased by the serum deprivation.SB203580, a specific inhibitor of p38MAPK, prevented both the serum deprivation−induced Bax expression and microglial death.Immunochemical staining showed that microglia expressing a high level of Bax were subjected to apoptosis−like cell death.These observations suggest that Bax expression underlies the apoptosis of cultured microglia after serum deprivation.
doi_str_mv 10.1254/jjp.83.351
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subjects Apoptosis
Bax
Microglia
title Serum-Deprivation Induces Cell Death of Rat Cultured Microglia Accompanied With Expression of Bax Protein
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