Fluorescent Estimation of H2O2-Induced Changes in Cell Viability and Cellular Nonprotein Thiol Level of Dissociated Rat Thymocytes

We have developed a procedure to simultaneously estimate cell viability and the cellular level of nonprotein thiol (presumably glutathione) using two fluorescent dyes, 5-chloromethylfluorescein (5CMF) and ethidium, and rat thymocytes. Diethylmaleate and N-ethylmaleimide reduced, respectively, the in...

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Veröffentlicht in:Japanese journal of pharmacology 1996, Vol.71(4), pp.299-305
Hauptverfasser: Chikahisa, Lumi, Oyama, Yasuo, Okazaki, Eisuke, Noda, Katsuhiko
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Sprache:eng
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