4-(2-Aminoethylamino)-7 H -benz[ de ]benzimidazo[2,1- a ]isoquinoline-7-one as a Highly Sensitive Fluorescent Labeling Reagent for Carnitine

4-(2-Aminoethylamino)-7 H -benz[ de ]benzimidazo[2,1- a ]isoquinoline-7-one as a Highly Sensitive Fluorescent Labeling Reagent for Carnitine

4-(2-Aminoethylamino)-7H-benz[de]benzimidazo[2,1-a]isoquinoline-7-one, synthesized and identified by a HMBC study, can be used as a fluorescent labeling reagent for carnitine. The excitation and emission maxima in acetonitrile were observed at λ 454 and 508 nm, respectively. This reagent smoothly re...

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Veröffentlicht in:Bulletin of the Chemical Society of Japan 2001-01, Vol.74 (1), p.173-177
Hauptverfasser: Nakaya, Ken-ichi, Tanaka, Toshiyuki, Shirataki, Yoshiaki, Shiozaki, Hisayoshi, Funabiki, Kazumasa, Shibata, Katsuyoshi, Matsui, Masaki
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container_end_page 177
container_issue 1
container_start_page 173
container_title Bulletin of the Chemical Society of Japan
container_volume 74
creator Nakaya, Ken-ichi
Tanaka, Toshiyuki
Shirataki, Yoshiaki
Shiozaki, Hisayoshi
Funabiki, Kazumasa
Shibata, Katsuyoshi
Matsui, Masaki
description 4-(2-Aminoethylamino)-7H-benz[de]benzimidazo[2,1-a]isoquinoline-7-one, synthesized and identified by a HMBC study, can be used as a fluorescent labeling reagent for carnitine. The excitation and emission maxima in acetonitrile were observed at λ 454 and 508 nm, respectively. This reagent smoothly reacted with carnitine in the presence of 1-[3-(dimethylamino)propyl]-3-ethylcarbodiimide hydrochloride to afford the corresponding amide under mild conditions. The detection limit (S/N > 3) of carnitine was ca. 12 fmol.
doi_str_mv 10.1246/bcsj.74.173
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title 4-(2-Aminoethylamino)-7 H -benz[ de ]benzimidazo[2,1- a ]isoquinoline-7-one as a Highly Sensitive Fluorescent Labeling Reagent for Carnitine
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