The atypical histone variant H3.15 promotes callus formation in Arabidopsis thaliana
Plants are capable of regenerating new organs after mechanical injury. The regeneration process involves genome-wide reprogramming of transcription, which usually requires dynamic changes in the chromatin landscape. We show that the histone 3 variant HISTONE THREE RELATED 15 (H3.15) plays an importa...
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| Veröffentlicht in: | Development (Cambridge) 2020-06, Vol.147 (11) |
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| creator | Yan, An Borg, Michael Berger, Frédéric Chen, Zhong |
| description | Plants are capable of regenerating new organs after mechanical injury. The regeneration process involves genome-wide reprogramming of transcription, which usually requires dynamic changes in the chromatin landscape. We show that the histone 3 variant HISTONE THREE RELATED 15 (H3.15) plays an important role in cell fate reprogramming during plant regeneration in
H3.15 expression is rapidly induced upon wounding. Ectopic overexpression of H3.15 promotes cell proliferation to form a larger callus at the wound site, whereas
mutation compromises callus formation. H3.15 is distinguished from other
histones by the absence of the lysine residue 27 that is trimethylated by the POLYCOMB REPRESSIVE COMPLEX 2 (PRC2) in constitutively expressed H3 variants. Overexpression of H3.15 promotes the removal of the transcriptional repressive mark H3K27me3 from chromatin, which results in transcriptional de-repression of downstream genes, such as
(
). Our results reveal a new mechanism for a release from PRC2-mediated gene repression through H3.15 deposition into chromatin, which is involved in reprogramming cell fate to produce pluripotent callus cells. |
| doi_str_mv | 10.1242/dev.184895 |
| format | Article |
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H3.15 expression is rapidly induced upon wounding. Ectopic overexpression of H3.15 promotes cell proliferation to form a larger callus at the wound site, whereas
mutation compromises callus formation. H3.15 is distinguished from other
histones by the absence of the lysine residue 27 that is trimethylated by the POLYCOMB REPRESSIVE COMPLEX 2 (PRC2) in constitutively expressed H3 variants. Overexpression of H3.15 promotes the removal of the transcriptional repressive mark H3K27me3 from chromatin, which results in transcriptional de-repression of downstream genes, such as
(
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H3.15 expression is rapidly induced upon wounding. Ectopic overexpression of H3.15 promotes cell proliferation to form a larger callus at the wound site, whereas
mutation compromises callus formation. H3.15 is distinguished from other
histones by the absence of the lysine residue 27 that is trimethylated by the POLYCOMB REPRESSIVE COMPLEX 2 (PRC2) in constitutively expressed H3 variants. Overexpression of H3.15 promotes the removal of the transcriptional repressive mark H3K27me3 from chromatin, which results in transcriptional de-repression of downstream genes, such as
(
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H3.15 expression is rapidly induced upon wounding. Ectopic overexpression of H3.15 promotes cell proliferation to form a larger callus at the wound site, whereas
mutation compromises callus formation. H3.15 is distinguished from other
histones by the absence of the lysine residue 27 that is trimethylated by the POLYCOMB REPRESSIVE COMPLEX 2 (PRC2) in constitutively expressed H3 variants. Overexpression of H3.15 promotes the removal of the transcriptional repressive mark H3K27me3 from chromatin, which results in transcriptional de-repression of downstream genes, such as
(
). Our results reveal a new mechanism for a release from PRC2-mediated gene repression through H3.15 deposition into chromatin, which is involved in reprogramming cell fate to produce pluripotent callus cells.</abstract><cop>England</cop><pmid>32439757</pmid><doi>10.1242/dev.184895</doi><orcidid>https://orcid.org/0000-0003-1632-1389</orcidid><oa>free_for_read</oa></addata></record> |
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| language | eng |
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| source | MEDLINE; EZB-FREE-00999 freely available EZB journals; Alma/SFX Local Collection; Company of Biologists |
| subjects | Amino Acid Sequence Arabidopsis - metabolism Arabidopsis Proteins - classification Arabidopsis Proteins - genetics Arabidopsis Proteins - metabolism Chromatin - metabolism Gene Expression Regulation, Plant Histones - classification Histones - genetics Histones - metabolism Homeodomain Proteins - genetics Homeodomain Proteins - metabolism Methylation Mutagenesis, Site-Directed Phylogeny Plants, Genetically Modified - metabolism Polycomb Repressive Complex 2 - metabolism Sequence Alignment Transcription Factors - genetics Transcription Factors - metabolism |
| title | The atypical histone variant H3.15 promotes callus formation in Arabidopsis thaliana |
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