Evaluation of ATN PD Framework and Biofluid Markers to Predict Cognitive Decline in Early Parkinson Disease

In Parkinson disease (PD), Alzheimer disease (AD) copathology is common and clinically relevant. However, the longitudinal progression of AD CSF biomarkers-β-amyloid 1-42 (Aβ ), phosphorylated tau 181 (p-tau ), and total tau (t-tau)-in PD is poorly understood and may be distinct from clinical AD. Moreover, it is unclear whether CSF p-tau and serum neurofilament light (NfL) have added prognostic utility in PD, when combined with CSF Aβ . First, we describe longitudinal trajectories of biofluid markers in PD. Second, we modified the AD β-amyloid/tau/neurodegeneration (ATN) framework for application in PD (ATN ) using CSF Aβ (A), p-tau (T), and serum NfL (N) and tested ATN prediction of longitudinal cognitive decline in PD. Participants were selected from the Parkinson's Progression Markers Initiative cohort, clinically diagnosed with sporadic PD or as controls, and followed up annually for 5 years. Linear mixed-effects models (LMEMs) tested the interaction of diagnosis with longitudinal trajectories of analytes (log transformed, false discovery rate [FDR] corrected). In patients with PD, LMEMs tested how baseline ATN status (AD [A+T+N±] vs not) predicted clinical outcomes, including Montreal Cognitive Assessment (MoCA; rank transformed, FDR corrected). Participants were 364 patients with PD and 168 controls, with comparable baseline mean (±SD) age (patients with PD = 62 ± 10 years; controls = 61 ± 11 years]; Mann-Whitney Wilcoxon: = 0.4) and sex distribution (patients with PD = 231 male individuals [63%]; controls = 107 male individuals [64%]; χ : = 1). Patients with PD had overall lower CSF p-tau (β = -0.16, 95% CI -0.23 to -0.092, = 2.2e-05) and t-tau than controls (β = -0.13, 95% CI -0.19 to -0.065, = 4e-04), but not Aβ ( = 0.061) or NfL ( = 0.32). Over time, patients with PD had greater increases in serum NfL than controls (β = 0.035, 95% CI 0.022 to 0.048, = 9.8e-07); slopes of patients with PD did not differ from those of controls for CSF Aβ ( = 0.18), p-tau ( = 1), or t-tau ( = 0.96). Using ATN , PD classified as A+T+N± (n = 32; 9%) had worse cognitive decline on global MoCA (β = -73, 95% CI -110 to -37, = 0.00077) than all other ATN statuses including A+ alone (A+T-N-; n = 75; 21%). In patients with early PD, CSF p-tau and t-tau were low compared with those in controls and did not increase over 5 years of follow-up. Our study shows that classification using modified ATN (incorporating CSF Aβ , CSF p-tau , and serum NfL) can identify biologically rele