A New Nonisotopic, Highly Sensitive Assay for the Measurement of Human Placental Growth Hormone: Development and Clinical Implications
Human placental GH (hGH-V) is a variant of pituitary hGH (hGH-N) synthesized and secreted by syncytiotrophoblasts during pregnancy. It differs from hGH-V by only 13 amino acid residues, which makes difficult a specific measurement of hGH-V without interference from hGH-N. To overcome the analytical...
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| Veröffentlicht in: | The journal of clinical endocrinology and metabolism 2003-02, Vol.88 (2), p.804-811 |
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| creator | Wu, Zida Bidlingmaier, Martin Friess, Stephanie C. Kirk, Susan E. Buchinger, Peter Schiessl, Barbara Strasburger, Christian J. |
| description | Human placental GH (hGH-V) is a variant of pituitary hGH (hGH-N) synthesized and secreted by syncytiotrophoblasts during pregnancy. It differs from hGH-V by only 13 amino acid residues, which makes difficult a specific measurement of hGH-V without interference from hGH-N. To overcome the analytical difficulties, we produced new high affinity monoclonal antibodies specific for hGH-V. Precise screening and epitope mapping allowed identification of a pair of monoclonal antibodies suitable to establish a highly sensitive assay for hGH-V measurement. In a prospective, longitudinal study involving 84 normal pregnancies, we measured maternal concentrations of hGH-V, leptin, IGF-I, and cord blood IGF-I. hGH-V was detectable as early as gestational week (GW) 7. Mean concentrations of hGH-V increased from 0.9 ± 0.5 μg/liter (GW 7–13) to 2.8 ± 0.9 μg/liter (GW 18–22), 7.3 ± 2.6 μg/liter (GW 28–32), and 13.0 ± 9.6 (GW 37–41). A negative correlation was found between prepregnancy body mass index and hGH-V concentrations from GW 28 onward. Peak hGH-V levels occurred at wk 36.5 ± 2.6 and were significantly lower in obese (P = 0.029) and higher in underweight (P = 0.035) mothers compared with those in mothers of normal weight. The increase in hGH-V between GW 18–22 and GW 28–32 was negatively correlated to the increase in maternal leptin during this period (P = 0.027). Maternal IGF-I concentrations were correlated to those of hGH-V from GW 18 onward (P = 0.039). The strongest correlation was found at GW 28–32 (P = 0.001). Furthermore, maternal hGH-V concentrations in late pregnancy correlated with cord blood IGF-I (P = 0.025) and size of the newborn (P = 0.017).
These results, obtained by a new, highly sensitive hGH-V-specific immunoassay, highlight the importance of maternal hGH-V in the regulation of maternal and fetal IGF-I. In addition, the results indicate that maternal weight has a major impact on circulating concentrations of hGH-V. |
| doi_str_mv | 10.1210/jc.2002-020787 |
| format | Article |
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These results, obtained by a new, highly sensitive hGH-V-specific immunoassay, highlight the importance of maternal hGH-V in the regulation of maternal and fetal IGF-I. In addition, the results indicate that maternal weight has a major impact on circulating concentrations of hGH-V.</description><identifier>ISSN: 0021-972X</identifier><identifier>EISSN: 1945-7197</identifier><identifier>DOI: 10.1210/jc.2002-020787</identifier><identifier>PMID: 12574217</identifier><identifier>CODEN: JCEMAZ</identifier><language>eng</language><publisher>Bethesda, MD: Endocrine Society</publisher><subject>Animals ; Antibodies, Monoclonal ; Antibody Specificity ; Biological and medical sciences ; Body Weight ; Epitope Mapping ; Female ; Fetal Blood ; Fibroblasts - physiology ; Functional investigation of endocrine glands and genital system ; Gene Expression ; Growth Hormone - analysis ; Growth Hormone - immunology ; Humans ; Immunoassay - methods ; Insulin-Like Growth Factor I - metabolism ; Investigative techniques, diagnostic techniques (general aspects) ; Leptin - blood ; Longitudinal Studies ; Medical sciences ; Mice ; Mice, Inbred BALB C ; Placental Hormones - analysis ; Placental Hormones - immunology ; Pregnancy ; Prospective Studies ; Reagent Kits, Diagnostic ; Recombinant Proteins - genetics ; Sensitivity and Specificity</subject><ispartof>The journal of clinical endocrinology and metabolism, 2003-02, Vol.88 (2), p.804-811</ispartof><rights>2003 INIST-CNRS</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c376t-2ff88bc0d79855c3795ad6892b0c3d648ad9df2f549d7fb3a7c1783ebff4f0cd3</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>315,781,785,27926,27927</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=14542812$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/12574217$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Wu, Zida</creatorcontrib><creatorcontrib>Bidlingmaier, Martin</creatorcontrib><creatorcontrib>Friess, Stephanie C.</creatorcontrib><creatorcontrib>Kirk, Susan E.</creatorcontrib><creatorcontrib>Buchinger, Peter</creatorcontrib><creatorcontrib>Schiessl, Barbara</creatorcontrib><creatorcontrib>Strasburger, Christian J.</creatorcontrib><title>A New Nonisotopic, Highly Sensitive Assay for the Measurement of Human Placental Growth Hormone: Development and Clinical Implications</title><title>The journal of clinical endocrinology and metabolism</title><addtitle>J Clin Endocrinol Metab</addtitle><description>Human placental GH (hGH-V) is a variant of pituitary hGH (hGH-N) synthesized and secreted by syncytiotrophoblasts during pregnancy. It differs from hGH-V by only 13 amino acid residues, which makes difficult a specific measurement of hGH-V without interference from hGH-N. To overcome the analytical difficulties, we produced new high affinity monoclonal antibodies specific for hGH-V. Precise screening and epitope mapping allowed identification of a pair of monoclonal antibodies suitable to establish a highly sensitive assay for hGH-V measurement. In a prospective, longitudinal study involving 84 normal pregnancies, we measured maternal concentrations of hGH-V, leptin, IGF-I, and cord blood IGF-I. hGH-V was detectable as early as gestational week (GW) 7. Mean concentrations of hGH-V increased from 0.9 ± 0.5 μg/liter (GW 7–13) to 2.8 ± 0.9 μg/liter (GW 18–22), 7.3 ± 2.6 μg/liter (GW 28–32), and 13.0 ± 9.6 (GW 37–41). A negative correlation was found between prepregnancy body mass index and hGH-V concentrations from GW 28 onward. Peak hGH-V levels occurred at wk 36.5 ± 2.6 and were significantly lower in obese (P = 0.029) and higher in underweight (P = 0.035) mothers compared with those in mothers of normal weight. The increase in hGH-V between GW 18–22 and GW 28–32 was negatively correlated to the increase in maternal leptin during this period (P = 0.027). Maternal IGF-I concentrations were correlated to those of hGH-V from GW 18 onward (P = 0.039). The strongest correlation was found at GW 28–32 (P = 0.001). Furthermore, maternal hGH-V concentrations in late pregnancy correlated with cord blood IGF-I (P = 0.025) and size of the newborn (P = 0.017).
These results, obtained by a new, highly sensitive hGH-V-specific immunoassay, highlight the importance of maternal hGH-V in the regulation of maternal and fetal IGF-I. In addition, the results indicate that maternal weight has a major impact on circulating concentrations of hGH-V.</description><subject>Animals</subject><subject>Antibodies, Monoclonal</subject><subject>Antibody Specificity</subject><subject>Biological and medical sciences</subject><subject>Body Weight</subject><subject>Epitope Mapping</subject><subject>Female</subject><subject>Fetal Blood</subject><subject>Fibroblasts - physiology</subject><subject>Functional investigation of endocrine glands and genital system</subject><subject>Gene Expression</subject><subject>Growth Hormone - analysis</subject><subject>Growth Hormone - immunology</subject><subject>Humans</subject><subject>Immunoassay - methods</subject><subject>Insulin-Like Growth Factor I - metabolism</subject><subject>Investigative techniques, diagnostic techniques (general aspects)</subject><subject>Leptin - blood</subject><subject>Longitudinal Studies</subject><subject>Medical sciences</subject><subject>Mice</subject><subject>Mice, Inbred BALB C</subject><subject>Placental Hormones - analysis</subject><subject>Placental Hormones - immunology</subject><subject>Pregnancy</subject><subject>Prospective Studies</subject><subject>Reagent Kits, Diagnostic</subject><subject>Recombinant Proteins - genetics</subject><subject>Sensitivity and Specificity</subject><issn>0021-972X</issn><issn>1945-7197</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2003</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp1kEFPGzEQha2qVQmh1x4rX3rrprbXjnd7i1IgSDQgAVJvK689bhzt2it7A8of4HdjSCROPc1o5nujeQ-hr5TMKKPk51bPGCGsIIzISn5AE1pzUUhay49okhe0qCX7e4JOU9oSQjkX5Wd0QpmQnFE5Qc8LvIYnvA7epTCGwekfeOX-bbo9vgOf3OgeAS9SUntsQ8TjBvAfUGkXoQc_4mDxatcrj287pfNAdfgyhqdxg1ch9sHDL_wbHqELwxuuvMHLznmnM3jVD11uRhd8OkOfrOoSfDnWKXq4OL9frorrm8ur5eK60KWcjwWztqpaTYysKyHyrBbKzKuatUSXZs4rZWpjmRW8NtK2pZKayqqE1lpuiTblFM0Od3UMKUWwzRBdr-K-oaR5DbTZ6uY10OYQaBZ8OwiGXduDecePCWbg-xFQKbuyUXnt0jvHBWcVZZkTBw68CTo6D0OElJpt2EWfLf_vgReGyZHy</recordid><startdate>20030201</startdate><enddate>20030201</enddate><creator>Wu, Zida</creator><creator>Bidlingmaier, Martin</creator><creator>Friess, Stephanie C.</creator><creator>Kirk, Susan E.</creator><creator>Buchinger, Peter</creator><creator>Schiessl, Barbara</creator><creator>Strasburger, Christian J.</creator><general>Endocrine Society</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope></search><sort><creationdate>20030201</creationdate><title>A New Nonisotopic, Highly Sensitive Assay for the Measurement of Human Placental Growth Hormone: Development and Clinical Implications</title><author>Wu, Zida ; Bidlingmaier, Martin ; Friess, Stephanie C. ; Kirk, Susan E. ; Buchinger, Peter ; Schiessl, Barbara ; Strasburger, Christian J.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c376t-2ff88bc0d79855c3795ad6892b0c3d648ad9df2f549d7fb3a7c1783ebff4f0cd3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2003</creationdate><topic>Animals</topic><topic>Antibodies, Monoclonal</topic><topic>Antibody Specificity</topic><topic>Biological and medical sciences</topic><topic>Body Weight</topic><topic>Epitope Mapping</topic><topic>Female</topic><topic>Fetal Blood</topic><topic>Fibroblasts - physiology</topic><topic>Functional investigation of endocrine glands and genital system</topic><topic>Gene Expression</topic><topic>Growth Hormone - analysis</topic><topic>Growth Hormone - immunology</topic><topic>Humans</topic><topic>Immunoassay - methods</topic><topic>Insulin-Like Growth Factor I - metabolism</topic><topic>Investigative techniques, diagnostic techniques (general aspects)</topic><topic>Leptin - blood</topic><topic>Longitudinal Studies</topic><topic>Medical sciences</topic><topic>Mice</topic><topic>Mice, Inbred BALB C</topic><topic>Placental Hormones - analysis</topic><topic>Placental Hormones - immunology</topic><topic>Pregnancy</topic><topic>Prospective Studies</topic><topic>Reagent Kits, Diagnostic</topic><topic>Recombinant Proteins - genetics</topic><topic>Sensitivity and Specificity</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Wu, Zida</creatorcontrib><creatorcontrib>Bidlingmaier, Martin</creatorcontrib><creatorcontrib>Friess, Stephanie C.</creatorcontrib><creatorcontrib>Kirk, Susan E.</creatorcontrib><creatorcontrib>Buchinger, Peter</creatorcontrib><creatorcontrib>Schiessl, Barbara</creatorcontrib><creatorcontrib>Strasburger, Christian J.</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><jtitle>The journal of clinical endocrinology and metabolism</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Wu, Zida</au><au>Bidlingmaier, Martin</au><au>Friess, Stephanie C.</au><au>Kirk, Susan E.</au><au>Buchinger, Peter</au><au>Schiessl, Barbara</au><au>Strasburger, Christian J.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>A New Nonisotopic, Highly Sensitive Assay for the Measurement of Human Placental Growth Hormone: Development and Clinical Implications</atitle><jtitle>The journal of clinical endocrinology and metabolism</jtitle><addtitle>J Clin Endocrinol Metab</addtitle><date>2003-02-01</date><risdate>2003</risdate><volume>88</volume><issue>2</issue><spage>804</spage><epage>811</epage><pages>804-811</pages><issn>0021-972X</issn><eissn>1945-7197</eissn><coden>JCEMAZ</coden><abstract>Human placental GH (hGH-V) is a variant of pituitary hGH (hGH-N) synthesized and secreted by syncytiotrophoblasts during pregnancy. It differs from hGH-V by only 13 amino acid residues, which makes difficult a specific measurement of hGH-V without interference from hGH-N. To overcome the analytical difficulties, we produced new high affinity monoclonal antibodies specific for hGH-V. Precise screening and epitope mapping allowed identification of a pair of monoclonal antibodies suitable to establish a highly sensitive assay for hGH-V measurement. In a prospective, longitudinal study involving 84 normal pregnancies, we measured maternal concentrations of hGH-V, leptin, IGF-I, and cord blood IGF-I. hGH-V was detectable as early as gestational week (GW) 7. Mean concentrations of hGH-V increased from 0.9 ± 0.5 μg/liter (GW 7–13) to 2.8 ± 0.9 μg/liter (GW 18–22), 7.3 ± 2.6 μg/liter (GW 28–32), and 13.0 ± 9.6 (GW 37–41). A negative correlation was found between prepregnancy body mass index and hGH-V concentrations from GW 28 onward. Peak hGH-V levels occurred at wk 36.5 ± 2.6 and were significantly lower in obese (P = 0.029) and higher in underweight (P = 0.035) mothers compared with those in mothers of normal weight. The increase in hGH-V between GW 18–22 and GW 28–32 was negatively correlated to the increase in maternal leptin during this period (P = 0.027). Maternal IGF-I concentrations were correlated to those of hGH-V from GW 18 onward (P = 0.039). The strongest correlation was found at GW 28–32 (P = 0.001). Furthermore, maternal hGH-V concentrations in late pregnancy correlated with cord blood IGF-I (P = 0.025) and size of the newborn (P = 0.017).
These results, obtained by a new, highly sensitive hGH-V-specific immunoassay, highlight the importance of maternal hGH-V in the regulation of maternal and fetal IGF-I. In addition, the results indicate that maternal weight has a major impact on circulating concentrations of hGH-V.</abstract><cop>Bethesda, MD</cop><pub>Endocrine Society</pub><pmid>12574217</pmid><doi>10.1210/jc.2002-020787</doi><tpages>8</tpages><oa>free_for_read</oa></addata></record> |
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| source | MEDLINE; Oxford University Press Journals All Titles (1996-Current); EZB-FREE-00999 freely available EZB journals |
| subjects | Animals Antibodies, Monoclonal Antibody Specificity Biological and medical sciences Body Weight Epitope Mapping Female Fetal Blood Fibroblasts - physiology Functional investigation of endocrine glands and genital system Gene Expression Growth Hormone - analysis Growth Hormone - immunology Humans Immunoassay - methods Insulin-Like Growth Factor I - metabolism Investigative techniques, diagnostic techniques (general aspects) Leptin - blood Longitudinal Studies Medical sciences Mice Mice, Inbred BALB C Placental Hormones - analysis Placental Hormones - immunology Pregnancy Prospective Studies Reagent Kits, Diagnostic Recombinant Proteins - genetics Sensitivity and Specificity |
| title | A New Nonisotopic, Highly Sensitive Assay for the Measurement of Human Placental Growth Hormone: Development and Clinical Implications |
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