1396 Epidemiology and Antibiotic Susceptibility Spectrum in Upec From Urine in Children Suspected to Uti in Mofid Children Hospital

Introduction: Urinary tract infection due to UPEC with antibiotic resistance is one of the most important problems in infants and children. Prevalence of UPEC isolated from children urine samples and their antimicrobial susceptibilities were considered in this study. Material and Methods: Urine samples of children who referred to Mofeed hospital were studied during one year. E.coli strains in these urine samples were identified by conventional methods. The Uropathogenic E.coli strains was confirmed by the gene including by decteting papC, papGII, papGIII, sfa/foc, hlyC, c nf1, iucC, fyuA, iron N genes by PCR method. Results: 12572 urine samples of suspected children to have urinary infections were studied and then 378 E.coli strains were detected in which 150 of strains were UPEC (39/7%). All of Uropathogenic E.coli were resistant to penicillin, Oxacillin, Bacit racin, Cloxacillinand pipracillin.resistantto other antibiotics were: Sulfametoxazole 92%, Nalidixic acid 53%, Ampicillin 89%, Nitrafurantoin 9%, Cephotaxime55.3%, Cefixime67%, Gentamicin72% Cephalexin75.6%, Ciprofloxacin17.5%. Conclusion: Periodic review and formulation of antiobiotic policy are needed for control of acquisition of drug resistance. Further studies on better understanding of interaction of different virulence factors at molecular level are necessary as most urovirulent strain express multiple virulence factors simultaneously.