Lung exposure to nanoparticles modulates an asthmatic response in a mouse model
The aim of this study was to investigate the modulation of an asthmatic response by titanium dioxide (TiO₂) or gold (Au) nanoparticles (NPs) in a murine model of diisocyanate-induced asthma. On days 1 and 8, BALB/c mice received 0.3% toluene diisocyanate (TDI) or the vehicle acetone-olive oil (AOO)...
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| Veröffentlicht in: | The European respiratory journal 2011-02, Vol.37 (2), p.299-309 |
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| creator | HUSSAIN, S VANOIRBEEK, J. A. J NEMERY, B HOET, P. H. M LUYTS, K DE VOOGHT, V VERBEKEN, E THOMASSEN, L. C. J MARTENS, J. A DINSDALE, D BOLAND, S MARANO, F |
| description | The aim of this study was to investigate the modulation of an asthmatic response by titanium dioxide (TiO₂) or gold (Au) nanoparticles (NPs) in a murine model of diisocyanate-induced asthma. On days 1 and 8, BALB/c mice received 0.3% toluene diisocyanate (TDI) or the vehicle acetone-olive oil (AOO) on the dorsum of both ears (20 μL). On day 14, the mice were oropharyngeally dosed with 40 μL of a NP suspension (0.4 mg·mL⁻¹ (∼0.8 mg·kg⁻¹) TiO₂ or Au). 1 day later (day 15), the mice received an oropharyngeal challenge with 0.01% TDI (20 μL). On day 16, airway hyperreactivity (AHR), bronchoalveolar lavage (BAL) cell and cytokine analysis, lung histology, and total serum immunoglobulin E were assessed. NP exposure in sensitised mice led to a two- (TiO₂) or three-fold (Au) increase in AHR, and a three- (TiO₂) or five-fold (Au) increase in BAL total cell counts, mainly comprising neutrophils and macrophages. The NPs taken up by BAL macrophages were identified by energy dispersive X-ray spectroscopy. Histological analysis revealed increased oedema, epithelial damage and inflammation. In conclusion, these results show that a low, intrapulmonary doses of TiO₂ or Au NPs can aggravate pulmonary inflammation and AHR in a mouse model of diisocyanate-induced asthma. |
| doi_str_mv | 10.1183/09031936.00168509 |
| format | Article |
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A. J ; NEMERY, B ; HOET, P. H. M ; LUYTS, K ; DE VOOGHT, V ; VERBEKEN, E ; THOMASSEN, L. C. J ; MARTENS, J. A ; DINSDALE, D ; BOLAND, S ; MARANO, F</creator><creatorcontrib>HUSSAIN, S ; VANOIRBEEK, J. A. J ; NEMERY, B ; HOET, P. H. M ; LUYTS, K ; DE VOOGHT, V ; VERBEKEN, E ; THOMASSEN, L. C. J ; MARTENS, J. A ; DINSDALE, D ; BOLAND, S ; MARANO, F</creatorcontrib><description>The aim of this study was to investigate the modulation of an asthmatic response by titanium dioxide (TiO₂) or gold (Au) nanoparticles (NPs) in a murine model of diisocyanate-induced asthma. On days 1 and 8, BALB/c mice received 0.3% toluene diisocyanate (TDI) or the vehicle acetone-olive oil (AOO) on the dorsum of both ears (20 μL). On day 14, the mice were oropharyngeally dosed with 40 μL of a NP suspension (0.4 mg·mL⁻¹ (∼0.8 mg·kg⁻¹) TiO₂ or Au). 1 day later (day 15), the mice received an oropharyngeal challenge with 0.01% TDI (20 μL). On day 16, airway hyperreactivity (AHR), bronchoalveolar lavage (BAL) cell and cytokine analysis, lung histology, and total serum immunoglobulin E were assessed. NP exposure in sensitised mice led to a two- (TiO₂) or three-fold (Au) increase in AHR, and a three- (TiO₂) or five-fold (Au) increase in BAL total cell counts, mainly comprising neutrophils and macrophages. The NPs taken up by BAL macrophages were identified by energy dispersive X-ray spectroscopy. Histological analysis revealed increased oedema, epithelial damage and inflammation. In conclusion, these results show that a low, intrapulmonary doses of TiO₂ or Au NPs can aggravate pulmonary inflammation and AHR in a mouse model of diisocyanate-induced asthma.</description><identifier>ISSN: 0903-1936</identifier><identifier>EISSN: 1399-3003</identifier><identifier>DOI: 10.1183/09031936.00168509</identifier><identifier>PMID: 20530043</identifier><language>eng</language><publisher>Leeds: Maney</publisher><subject>Animals ; Asthma - chemically induced ; Asthma - physiopathology ; Biological and medical sciences ; Bronchial Hyperreactivity ; Bronchoalveolar Lavage Fluid ; Chronic obstructive pulmonary disease, asthma ; Disease Models, Animal ; Eosinophils ; Gold - adverse effects ; Immunoglobulin E - blood ; Lung - physiopathology ; Macrophages ; Male ; Medical sciences ; Mice ; Mice, Inbred BALB C ; Nanoparticles - adverse effects ; Neutrophils ; Pneumology ; Pulmonary Edema - chemically induced ; Pulmonary Edema - physiopathology ; Titanium - adverse effects ; Toluene 2,4-Diisocyanate - toxicity</subject><ispartof>The European respiratory journal, 2011-02, Vol.37 (2), p.299-309</ispartof><rights>2015 INIST-CNRS</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c373t-5aa32ffa64907dcf1dfdf9e81797de2c815359bb5848a704056601776cda66a53</citedby><cites>FETCH-LOGICAL-c373t-5aa32ffa64907dcf1dfdf9e81797de2c815359bb5848a704056601776cda66a53</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,777,781,27905,27906</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=24090931$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/20530043$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>HUSSAIN, S</creatorcontrib><creatorcontrib>VANOIRBEEK, J. A. J</creatorcontrib><creatorcontrib>NEMERY, B</creatorcontrib><creatorcontrib>HOET, P. H. M</creatorcontrib><creatorcontrib>LUYTS, K</creatorcontrib><creatorcontrib>DE VOOGHT, V</creatorcontrib><creatorcontrib>VERBEKEN, E</creatorcontrib><creatorcontrib>THOMASSEN, L. C. J</creatorcontrib><creatorcontrib>MARTENS, J. A</creatorcontrib><creatorcontrib>DINSDALE, D</creatorcontrib><creatorcontrib>BOLAND, S</creatorcontrib><creatorcontrib>MARANO, F</creatorcontrib><title>Lung exposure to nanoparticles modulates an asthmatic response in a mouse model</title><title>The European respiratory journal</title><addtitle>Eur Respir J</addtitle><description>The aim of this study was to investigate the modulation of an asthmatic response by titanium dioxide (TiO₂) or gold (Au) nanoparticles (NPs) in a murine model of diisocyanate-induced asthma. On days 1 and 8, BALB/c mice received 0.3% toluene diisocyanate (TDI) or the vehicle acetone-olive oil (AOO) on the dorsum of both ears (20 μL). On day 14, the mice were oropharyngeally dosed with 40 μL of a NP suspension (0.4 mg·mL⁻¹ (∼0.8 mg·kg⁻¹) TiO₂ or Au). 1 day later (day 15), the mice received an oropharyngeal challenge with 0.01% TDI (20 μL). On day 16, airway hyperreactivity (AHR), bronchoalveolar lavage (BAL) cell and cytokine analysis, lung histology, and total serum immunoglobulin E were assessed. NP exposure in sensitised mice led to a two- (TiO₂) or three-fold (Au) increase in AHR, and a three- (TiO₂) or five-fold (Au) increase in BAL total cell counts, mainly comprising neutrophils and macrophages. The NPs taken up by BAL macrophages were identified by energy dispersive X-ray spectroscopy. Histological analysis revealed increased oedema, epithelial damage and inflammation. In conclusion, these results show that a low, intrapulmonary doses of TiO₂ or Au NPs can aggravate pulmonary inflammation and AHR in a mouse model of diisocyanate-induced asthma.</description><subject>Animals</subject><subject>Asthma - chemically induced</subject><subject>Asthma - physiopathology</subject><subject>Biological and medical sciences</subject><subject>Bronchial Hyperreactivity</subject><subject>Bronchoalveolar Lavage Fluid</subject><subject>Chronic obstructive pulmonary disease, asthma</subject><subject>Disease Models, Animal</subject><subject>Eosinophils</subject><subject>Gold - adverse effects</subject><subject>Immunoglobulin E - blood</subject><subject>Lung - physiopathology</subject><subject>Macrophages</subject><subject>Male</subject><subject>Medical sciences</subject><subject>Mice</subject><subject>Mice, Inbred BALB C</subject><subject>Nanoparticles - adverse effects</subject><subject>Neutrophils</subject><subject>Pneumology</subject><subject>Pulmonary Edema - chemically induced</subject><subject>Pulmonary Edema - physiopathology</subject><subject>Titanium - adverse effects</subject><subject>Toluene 2,4-Diisocyanate - toxicity</subject><issn>0903-1936</issn><issn>1399-3003</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2011</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNpFkEtPwzAQhC0EoqXwA7ggXzim2N3Yjo-oooBUqRc4R1s_ICgv2YkE_x5HbeG0o51v5jCE3HK25LyAB6YZcA1yyRiXhWD6jMw5aJ0BY3BO5pOfTcCMXMX4NVE58EsyWzGRkBzmZLcd2w_qvvsujsHRoaMttl2PYahM7SJtOjvWOCSFLcU4fDaYHBpc7Ls2Olqlb4LGJBPq6mty4bGO7uZ4F-R98_S2fsm2u-fX9eM2M6BgyAQirLxHmWumrPHceuu1K7jSyrqVKbgAofd7UeQFKpYzISXjSkljUUoUsCD80GtCF2NwvuxD1WD4KTkrp3HK0zjlaZyUuTtk-nHfOPuXOK2RgPsjgNFg7QO2por_XJ46NXD4BfwobDg</recordid><startdate>20110201</startdate><enddate>20110201</enddate><creator>HUSSAIN, S</creator><creator>VANOIRBEEK, J. A. J</creator><creator>NEMERY, B</creator><creator>HOET, P. H. M</creator><creator>LUYTS, K</creator><creator>DE VOOGHT, V</creator><creator>VERBEKEN, E</creator><creator>THOMASSEN, L. C. J</creator><creator>MARTENS, J. A</creator><creator>DINSDALE, D</creator><creator>BOLAND, S</creator><creator>MARANO, F</creator><general>Maney</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope></search><sort><creationdate>20110201</creationdate><title>Lung exposure to nanoparticles modulates an asthmatic response in a mouse model</title><author>HUSSAIN, S ; VANOIRBEEK, J. A. J ; NEMERY, B ; HOET, P. H. M ; LUYTS, K ; DE VOOGHT, V ; VERBEKEN, E ; THOMASSEN, L. C. J ; MARTENS, J. A ; DINSDALE, D ; BOLAND, S ; MARANO, F</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c373t-5aa32ffa64907dcf1dfdf9e81797de2c815359bb5848a704056601776cda66a53</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2011</creationdate><topic>Animals</topic><topic>Asthma - chemically induced</topic><topic>Asthma - physiopathology</topic><topic>Biological and medical sciences</topic><topic>Bronchial Hyperreactivity</topic><topic>Bronchoalveolar Lavage Fluid</topic><topic>Chronic obstructive pulmonary disease, asthma</topic><topic>Disease Models, Animal</topic><topic>Eosinophils</topic><topic>Gold - adverse effects</topic><topic>Immunoglobulin E - blood</topic><topic>Lung - physiopathology</topic><topic>Macrophages</topic><topic>Male</topic><topic>Medical sciences</topic><topic>Mice</topic><topic>Mice, Inbred BALB C</topic><topic>Nanoparticles - adverse effects</topic><topic>Neutrophils</topic><topic>Pneumology</topic><topic>Pulmonary Edema - chemically induced</topic><topic>Pulmonary Edema - physiopathology</topic><topic>Titanium - adverse effects</topic><topic>Toluene 2,4-Diisocyanate - toxicity</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>HUSSAIN, S</creatorcontrib><creatorcontrib>VANOIRBEEK, J. A. J</creatorcontrib><creatorcontrib>NEMERY, B</creatorcontrib><creatorcontrib>HOET, P. H. M</creatorcontrib><creatorcontrib>LUYTS, K</creatorcontrib><creatorcontrib>DE VOOGHT, V</creatorcontrib><creatorcontrib>VERBEKEN, E</creatorcontrib><creatorcontrib>THOMASSEN, L. C. J</creatorcontrib><creatorcontrib>MARTENS, J. A</creatorcontrib><creatorcontrib>DINSDALE, D</creatorcontrib><creatorcontrib>BOLAND, S</creatorcontrib><creatorcontrib>MARANO, F</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><jtitle>The European respiratory journal</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>HUSSAIN, S</au><au>VANOIRBEEK, J. A. J</au><au>NEMERY, B</au><au>HOET, P. H. M</au><au>LUYTS, K</au><au>DE VOOGHT, V</au><au>VERBEKEN, E</au><au>THOMASSEN, L. C. J</au><au>MARTENS, J. A</au><au>DINSDALE, D</au><au>BOLAND, S</au><au>MARANO, F</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Lung exposure to nanoparticles modulates an asthmatic response in a mouse model</atitle><jtitle>The European respiratory journal</jtitle><addtitle>Eur Respir J</addtitle><date>2011-02-01</date><risdate>2011</risdate><volume>37</volume><issue>2</issue><spage>299</spage><epage>309</epage><pages>299-309</pages><issn>0903-1936</issn><eissn>1399-3003</eissn><abstract>The aim of this study was to investigate the modulation of an asthmatic response by titanium dioxide (TiO₂) or gold (Au) nanoparticles (NPs) in a murine model of diisocyanate-induced asthma. On days 1 and 8, BALB/c mice received 0.3% toluene diisocyanate (TDI) or the vehicle acetone-olive oil (AOO) on the dorsum of both ears (20 μL). On day 14, the mice were oropharyngeally dosed with 40 μL of a NP suspension (0.4 mg·mL⁻¹ (∼0.8 mg·kg⁻¹) TiO₂ or Au). 1 day later (day 15), the mice received an oropharyngeal challenge with 0.01% TDI (20 μL). On day 16, airway hyperreactivity (AHR), bronchoalveolar lavage (BAL) cell and cytokine analysis, lung histology, and total serum immunoglobulin E were assessed. NP exposure in sensitised mice led to a two- (TiO₂) or three-fold (Au) increase in AHR, and a three- (TiO₂) or five-fold (Au) increase in BAL total cell counts, mainly comprising neutrophils and macrophages. The NPs taken up by BAL macrophages were identified by energy dispersive X-ray spectroscopy. Histological analysis revealed increased oedema, epithelial damage and inflammation. In conclusion, these results show that a low, intrapulmonary doses of TiO₂ or Au NPs can aggravate pulmonary inflammation and AHR in a mouse model of diisocyanate-induced asthma.</abstract><cop>Leeds</cop><pub>Maney</pub><pmid>20530043</pmid><doi>10.1183/09031936.00168509</doi><tpages>11</tpages><oa>free_for_read</oa></addata></record> |
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| subjects | Animals Asthma - chemically induced Asthma - physiopathology Biological and medical sciences Bronchial Hyperreactivity Bronchoalveolar Lavage Fluid Chronic obstructive pulmonary disease, asthma Disease Models, Animal Eosinophils Gold - adverse effects Immunoglobulin E - blood Lung - physiopathology Macrophages Male Medical sciences Mice Mice, Inbred BALB C Nanoparticles - adverse effects Neutrophils Pneumology Pulmonary Edema - chemically induced Pulmonary Edema - physiopathology Titanium - adverse effects Toluene 2,4-Diisocyanate - toxicity |
| title | Lung exposure to nanoparticles modulates an asthmatic response in a mouse model |
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