Improved peroxyl radical scavenging TOSC assay to quantify antioxidant capacity using SIFT-MS
We report a new, fast, sensitive variation of the total oxyradical scavenging capacity (TOSC) assay for measuring the antioxidant capacity of pure compounds, plant extracts and biological fluids using selected ion flow tube mass spectrometry (SIFT-MS). The TOSC assay examines the partial inhibition...
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| Veröffentlicht in: | Redox report : communications in free radical research 2009-10, Vol.14 (5), p.197-204 |
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| container_title | Redox report : communications in free radical research |
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| creator | Senthilmohan, Senti T. Davis, Brett M Wilson, Paul F. McEwan, Murray J. |
| description | We report a new, fast, sensitive variation of the total oxyradical scavenging capacity (TOSC) assay for measuring the antioxidant capacity of pure compounds, plant extracts and biological fluids using selected ion flow tube mass spectrometry (SIFT-MS). The TOSC assay examines the partial inhibition of ethene formation in the presence of antioxidants that compete with α-keto-γ-methiolbutyric acid (KMBA) for reactive oxygen species. The SIFT-MS-TOSC assay takes 15 s for each ethene analysis and the time interval between consecutive analyses is 20 s. We demonstrate the method by monitoring the antioxidant capacity of several standard radical scavengers of peroxyl radicals. For peroxyl radicals the measured SIFT-MS-TOSC concentrations necessary to produce 50% inhibition of radical reaction with KMBA are 6.1 ± 0.3 μM for Trolox, 5.7 ± 0.3 μM for ascorbic acid, 8.4 ± 0.4 μM for uric acid and 38 ± 2 μM for reduced glutathione. |
| doi_str_mv | 10.1179/135100009X12525712409571 |
| format | Article |
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The TOSC assay examines the partial inhibition of ethene formation in the presence of antioxidants that compete with α-keto-γ-methiolbutyric acid (KMBA) for reactive oxygen species. The SIFT-MS-TOSC assay takes 15 s for each ethene analysis and the time interval between consecutive analyses is 20 s. We demonstrate the method by monitoring the antioxidant capacity of several standard radical scavengers of peroxyl radicals. For peroxyl radicals the measured SIFT-MS-TOSC concentrations necessary to produce 50% inhibition of radical reaction with KMBA are 6.1 ± 0.3 μM for Trolox, 5.7 ± 0.3 μM for ascorbic acid, 8.4 ± 0.4 μM for uric acid and 38 ± 2 μM for reduced glutathione.</description><identifier>ISSN: 1351-0002</identifier><identifier>EISSN: 1743-2928</identifier><identifier>DOI: 10.1179/135100009X12525712409571</identifier><identifier>PMID: 19843374</identifier><language>eng</language><publisher>England: Taylor & Francis</publisher><subject>Animals ; ANTIOXIDANT CAPACITY ; Antioxidants - analysis ; Ascorbic Acid - analysis ; Ascorbic Acid - blood ; Butyrates - chemistry ; Flavonoids - analysis ; Flavonoids - blood ; Glutathione - analysis ; Glutathione - blood ; Humans ; Mass Spectrometry - instrumentation ; Mass Spectrometry - methods ; Plant Extracts - analysis ; Reactive Oxygen Species - metabolism ; SIFT-MS ; Sulfhydryl Compounds ; TOSC ASSAY ; Uric Acid - analysis ; Uric Acid - blood</subject><ispartof>Redox report : communications in free radical research, 2009-10, Vol.14 (5), p.197-204</ispartof><rights>2009 Maney Publishing 2009</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c374t-7da701aab41357acc694ee06c7e25e71deaf67f77ea65d358d227a34edb987c43</citedby><cites>FETCH-LOGICAL-c374t-7da701aab41357acc694ee06c7e25e71deaf67f77ea65d358d227a34edb987c43</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,776,780,27901,27902</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/19843374$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Senthilmohan, Senti T.</creatorcontrib><creatorcontrib>Davis, Brett M</creatorcontrib><creatorcontrib>Wilson, Paul F.</creatorcontrib><creatorcontrib>McEwan, Murray J.</creatorcontrib><title>Improved peroxyl radical scavenging TOSC assay to quantify antioxidant capacity using SIFT-MS</title><title>Redox report : communications in free radical research</title><addtitle>Redox Rep</addtitle><description>We report a new, fast, sensitive variation of the total oxyradical scavenging capacity (TOSC) assay for measuring the antioxidant capacity of pure compounds, plant extracts and biological fluids using selected ion flow tube mass spectrometry (SIFT-MS). The TOSC assay examines the partial inhibition of ethene formation in the presence of antioxidants that compete with α-keto-γ-methiolbutyric acid (KMBA) for reactive oxygen species. The SIFT-MS-TOSC assay takes 15 s for each ethene analysis and the time interval between consecutive analyses is 20 s. We demonstrate the method by monitoring the antioxidant capacity of several standard radical scavengers of peroxyl radicals. For peroxyl radicals the measured SIFT-MS-TOSC concentrations necessary to produce 50% inhibition of radical reaction with KMBA are 6.1 ± 0.3 μM for Trolox, 5.7 ± 0.3 μM for ascorbic acid, 8.4 ± 0.4 μM for uric acid and 38 ± 2 μM for reduced glutathione.</description><subject>Animals</subject><subject>ANTIOXIDANT CAPACITY</subject><subject>Antioxidants - analysis</subject><subject>Ascorbic Acid - analysis</subject><subject>Ascorbic Acid - blood</subject><subject>Butyrates - chemistry</subject><subject>Flavonoids - analysis</subject><subject>Flavonoids - blood</subject><subject>Glutathione - analysis</subject><subject>Glutathione - blood</subject><subject>Humans</subject><subject>Mass Spectrometry - instrumentation</subject><subject>Mass Spectrometry - methods</subject><subject>Plant Extracts - analysis</subject><subject>Reactive Oxygen Species - metabolism</subject><subject>SIFT-MS</subject><subject>Sulfhydryl Compounds</subject><subject>TOSC ASSAY</subject><subject>Uric Acid - analysis</subject><subject>Uric Acid - blood</subject><issn>1351-0002</issn><issn>1743-2928</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2009</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkL1OwzAUhS0EolB4BeSNKRDbSZyMKKJQqahDi8SColvbqYySOLWT0rw9jlqJCXGXc4fv3J-DECbhAyE8eyQsJqGv7IPQmMac0CjMvJyhK8IjFtCMpue-91jgMTpB1859-Y4lWXqJJiRLI8Z4dIU-53VrzV5J3CprDkOFLUgtoMJOwF41W91s8Xq5yjE4BwPuDN710HS6HPAo5qClVyygBaG7AfdudKzms3XwtrpBFyVUTt2edIreZ8_r_DVYLF_m-dMiEP6ILuASeEgANpG_mIMQSRYpFSaCKxorTqSCMuEl5wqSWLI4lZRyYJGSmyzlImJTdH-c63_Z9cp1Ra2dUFUFjTK9Kzgb8yEs9GR6JIU1zllVFq3VNdihIGExZlv8la233p2W9JtayV_jKUwP5EdAN6WxNXwbW8mig6EytrTQCO0K9u-aHyLridg</recordid><startdate>20091001</startdate><enddate>20091001</enddate><creator>Senthilmohan, Senti T.</creator><creator>Davis, Brett M</creator><creator>Wilson, Paul F.</creator><creator>McEwan, Murray J.</creator><general>Taylor & Francis</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>20091001</creationdate><title>Improved peroxyl radical scavenging TOSC assay to quantify antioxidant capacity using SIFT-MS</title><author>Senthilmohan, Senti T. ; Davis, Brett M ; Wilson, Paul F. ; McEwan, Murray J.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c374t-7da701aab41357acc694ee06c7e25e71deaf67f77ea65d358d227a34edb987c43</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2009</creationdate><topic>Animals</topic><topic>ANTIOXIDANT CAPACITY</topic><topic>Antioxidants - analysis</topic><topic>Ascorbic Acid - analysis</topic><topic>Ascorbic Acid - blood</topic><topic>Butyrates - chemistry</topic><topic>Flavonoids - analysis</topic><topic>Flavonoids - blood</topic><topic>Glutathione - analysis</topic><topic>Glutathione - blood</topic><topic>Humans</topic><topic>Mass Spectrometry - instrumentation</topic><topic>Mass Spectrometry - methods</topic><topic>Plant Extracts - analysis</topic><topic>Reactive Oxygen Species - metabolism</topic><topic>SIFT-MS</topic><topic>Sulfhydryl Compounds</topic><topic>TOSC ASSAY</topic><topic>Uric Acid - analysis</topic><topic>Uric Acid - blood</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Senthilmohan, Senti T.</creatorcontrib><creatorcontrib>Davis, Brett M</creatorcontrib><creatorcontrib>Wilson, Paul F.</creatorcontrib><creatorcontrib>McEwan, Murray J.</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Redox report : communications in free radical research</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Senthilmohan, Senti T.</au><au>Davis, Brett M</au><au>Wilson, Paul F.</au><au>McEwan, Murray J.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Improved peroxyl radical scavenging TOSC assay to quantify antioxidant capacity using SIFT-MS</atitle><jtitle>Redox report : communications in free radical research</jtitle><addtitle>Redox Rep</addtitle><date>2009-10-01</date><risdate>2009</risdate><volume>14</volume><issue>5</issue><spage>197</spage><epage>204</epage><pages>197-204</pages><issn>1351-0002</issn><eissn>1743-2928</eissn><abstract>We report a new, fast, sensitive variation of the total oxyradical scavenging capacity (TOSC) assay for measuring the antioxidant capacity of pure compounds, plant extracts and biological fluids using selected ion flow tube mass spectrometry (SIFT-MS). The TOSC assay examines the partial inhibition of ethene formation in the presence of antioxidants that compete with α-keto-γ-methiolbutyric acid (KMBA) for reactive oxygen species. The SIFT-MS-TOSC assay takes 15 s for each ethene analysis and the time interval between consecutive analyses is 20 s. We demonstrate the method by monitoring the antioxidant capacity of several standard radical scavengers of peroxyl radicals. For peroxyl radicals the measured SIFT-MS-TOSC concentrations necessary to produce 50% inhibition of radical reaction with KMBA are 6.1 ± 0.3 μM for Trolox, 5.7 ± 0.3 μM for ascorbic acid, 8.4 ± 0.4 μM for uric acid and 38 ± 2 μM for reduced glutathione.</abstract><cop>England</cop><pub>Taylor & Francis</pub><pmid>19843374</pmid><doi>10.1179/135100009X12525712409571</doi><tpages>8</tpages></addata></record> |
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| ispartof | Redox report : communications in free radical research, 2009-10, Vol.14 (5), p.197-204 |
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| source | MEDLINE; Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals; Alma/SFX Local Collection |
| subjects | Animals ANTIOXIDANT CAPACITY Antioxidants - analysis Ascorbic Acid - analysis Ascorbic Acid - blood Butyrates - chemistry Flavonoids - analysis Flavonoids - blood Glutathione - analysis Glutathione - blood Humans Mass Spectrometry - instrumentation Mass Spectrometry - methods Plant Extracts - analysis Reactive Oxygen Species - metabolism SIFT-MS Sulfhydryl Compounds TOSC ASSAY Uric Acid - analysis Uric Acid - blood |
| title | Improved peroxyl radical scavenging TOSC assay to quantify antioxidant capacity using SIFT-MS |
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