FREEZE-DRYING OF SMALL TISSUE SAMPLES AND THIN FROZEN SECTIONS BELOW -60{degrees}C A SIMPLE METHOD OF CRYOSORPTION PUMPING

FREEZE-DRYING OF SMALL TISSUE SAMPLES AND THIN FROZEN SECTIONS BELOW -60{degrees}C A SIMPLE METHOD OF CRYOSORPTION PUMPING

Freeze-drying rates for three tissues have been determined by direct measurement of weight loss in a closed system, using an electrobalance. The half-drying times (T/2) at approximately 1 x 10–5 mm Hg with Dry Ice slush as coolant were determined for brain, kidney and liver and found to be 100, 50 a...

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Veröffentlicht in:The journal of histochemistry and cytochemistry 1967-04, Vol.15 (4), p.243-251
Hauptverfasser: STUMPF, WALTER E, ROTH, LLOYD J
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container_title The journal of histochemistry and cytochemistry
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creator STUMPF, WALTER E
ROTH, LLOYD J
description Freeze-drying rates for three tissues have been determined by direct measurement of weight loss in a closed system, using an electrobalance. The half-drying times (T/2) at approximately 1 x 10–5 mm Hg with Dry Ice slush as coolant were determined for brain, kidney and liver and found to be 100, 50 and 30 hr, respectively, for 10-mg samples of tissue. A method for the freeze-drying of frozen sections in which the temperature of the tissue is maintained below –60°C throughout sectioning and drying is discussed. A simple portable, all glass, freeze-drying system utilizing cryosorption pumping is presented.
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title FREEZE-DRYING OF SMALL TISSUE SAMPLES AND THIN FROZEN SECTIONS BELOW -60{degrees}C A SIMPLE METHOD OF CRYOSORPTION PUMPING
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