HISTOCHEMICAL INVESTIGATIONS ON THE IN VIVO EFFECTS OF FLUORIDE ON TRICARBOXYLIC ACID CYCLE DEHYDROGENASES FROM PELARGONIUM ZONALE: PART II
In vivo effects of fluoride on tricarboxylic acid (TCA) cycle dehydrogenase enzymes of Pelargonium zonale were studied using p-nitro blue tetrazoleum chloride. Plants were exposed to 17 ppb HF, and enzyme activities in treated plants were compared to those in controls. Leaves of control plants were...
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Veröffentlicht in: | J. Histochem. Cytochem.; (United States) 1967-04, Vol.15 (4), p.202-206 |
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creator | LOVELACE, C. JAMES MILLER, GENE W |
description | In vivo effects of fluoride on tricarboxylic acid (TCA) cycle dehydrogenase enzymes of Pelargonium zonale were studied using p-nitro blue tetrazoleum chloride. Plants were exposed to 17 ppb HF, and enzyme activities in treated plants were compared to those in controls. Leaves of control plants were incubated in 5 x 10–3
M sodium fluoride. Injuries observed in fumigation and solution experiments were similar. Leaf tissue subjected to HF or sodium fluoride evidenced less succinic p-nitro blue tetrazoleum reductase activity than did control tissue. Other TCA cycle dehydrogenase enzymes were not observably affected by the fluoride concentrations used in these experiments. Excised leaves cultured in 5 x 10–3
M sodium fluoride exhibited less succinic p-nitro blue tetrazoleum reductase activity after 24 hr than did leaves cultured in 5 x 10–3
M sodium chloride. |
doi_str_mv | 10.1177/15.4.202 |
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M sodium fluoride. Injuries observed in fumigation and solution experiments were similar. Leaf tissue subjected to HF or sodium fluoride evidenced less succinic p-nitro blue tetrazoleum reductase activity than did control tissue. Other TCA cycle dehydrogenase enzymes were not observably affected by the fluoride concentrations used in these experiments. Excised leaves cultured in 5 x 10–3
M sodium fluoride exhibited less succinic p-nitro blue tetrazoleum reductase activity after 24 hr than did leaves cultured in 5 x 10–3
M sodium chloride.</description><identifier>ISSN: 0022-1554</identifier><identifier>EISSN: 1551-5044</identifier><identifier>DOI: 10.1177/15.4.202</identifier><identifier>PMID: 6033028</identifier><language>eng</language><publisher>London, England: Histochemical Soc</publisher><subject>560303 - Chemicals Metabolism & Toxicology- Plants- (-1987) ; ALKALI METAL COMPOUNDS ; CARBOXYLIC ACIDS ; ENZYME ACTIVITY ; ENZYMES ; FLUORIDES ; FLUORINE COMPOUNDS ; HALIDES ; HALOGEN COMPOUNDS ; HYDROFLUORIC ACID ; HYDROGEN COMPOUNDS ; IN VIVO ; INJURIES ; INORGANIC ACIDS ; LEAVES ; METABOLISM ; ORGANIC ACIDS ; ORGANIC COMPOUNDS ; ORNAMENTAL PLANTS ; OXIDOREDUCTASES ; PLANTS ; RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT ; SODIUM COMPOUNDS ; SODIUM FLUORIDES ; TOXICITY</subject><ispartof>J. Histochem. Cytochem.; (United States), 1967-04, Vol.15 (4), p.202-206</ispartof><rights>1967, THE HISTOCHEMICAL SOCIETY, INC.</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://journals.sagepub.com/doi/pdf/10.1177/15.4.202$$EPDF$$P50$$Gsage$$H</linktopdf><linktohtml>$$Uhttps://journals.sagepub.com/doi/10.1177/15.4.202$$EHTML$$P50$$Gsage$$H</linktohtml><link.rule.ids>314,780,784,885,21819,27924,27925,43621,43622</link.rule.ids><backlink>$$Uhttps://www.osti.gov/biblio/5434576$$D View this record in Osti.gov$$Hfree_for_read</backlink></links><search><creatorcontrib>LOVELACE, C. JAMES</creatorcontrib><creatorcontrib>MILLER, GENE W</creatorcontrib><creatorcontrib>Humboldt State College, Arcata, CA</creatorcontrib><title>HISTOCHEMICAL INVESTIGATIONS ON THE IN VIVO EFFECTS OF FLUORIDE ON TRICARBOXYLIC ACID CYCLE DEHYDROGENASES FROM PELARGONIUM ZONALE: PART II</title><title>J. Histochem. Cytochem.; (United States)</title><addtitle>J. Histochem. Cytochem</addtitle><description>In vivo effects of fluoride on tricarboxylic acid (TCA) cycle dehydrogenase enzymes of Pelargonium zonale were studied using p-nitro blue tetrazoleum chloride. Plants were exposed to 17 ppb HF, and enzyme activities in treated plants were compared to those in controls. Leaves of control plants were incubated in 5 x 10–3
M sodium fluoride. Injuries observed in fumigation and solution experiments were similar. Leaf tissue subjected to HF or sodium fluoride evidenced less succinic p-nitro blue tetrazoleum reductase activity than did control tissue. Other TCA cycle dehydrogenase enzymes were not observably affected by the fluoride concentrations used in these experiments. Excised leaves cultured in 5 x 10–3
M sodium fluoride exhibited less succinic p-nitro blue tetrazoleum reductase activity after 24 hr than did leaves cultured in 5 x 10–3
M sodium chloride.</description><subject>560303 - Chemicals Metabolism & Toxicology- Plants- (-1987)</subject><subject>ALKALI METAL COMPOUNDS</subject><subject>CARBOXYLIC ACIDS</subject><subject>ENZYME ACTIVITY</subject><subject>ENZYMES</subject><subject>FLUORIDES</subject><subject>FLUORINE COMPOUNDS</subject><subject>HALIDES</subject><subject>HALOGEN COMPOUNDS</subject><subject>HYDROFLUORIC ACID</subject><subject>HYDROGEN COMPOUNDS</subject><subject>IN VIVO</subject><subject>INJURIES</subject><subject>INORGANIC ACIDS</subject><subject>LEAVES</subject><subject>METABOLISM</subject><subject>ORGANIC ACIDS</subject><subject>ORGANIC COMPOUNDS</subject><subject>ORNAMENTAL PLANTS</subject><subject>OXIDOREDUCTASES</subject><subject>PLANTS</subject><subject>RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT</subject><subject>SODIUM COMPOUNDS</subject><subject>SODIUM FLUORIDES</subject><subject>TOXICITY</subject><issn>0022-1554</issn><issn>1551-5044</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1967</creationdate><recordtype>article</recordtype><recordid>eNp90F1L6zAYB_Agis4X8CMEQfCmM0mTvnhXu3QNdI203XDehKymrrIXaSbjfIbzpU89Ey-9euDP73l4-ANwjdEQY9-_x2xIhwSRIzDAjGGHIUqPwQAhQpw-oGfg3Np3hDClLDgFpx5yXUSCAfibirKScconIo4yKPIZLysxjioh8xLKHFYp71M4EzMJeZLwuOrjBCbZVBZixP-Tot8tHuXzPBMxjGIxgvE8zjgc8XQ-KuSY51HJS5gUcgKfeBYVY5mL6QS-yDzK-AN8iooKCnEJThq9subqe16AacKrOHUyOf76zqkxo8TxDNEspDTwCfa0ftWBoUyHoR_gxtTGIxqzhed7TYgWuEZGN-6r9kJD_YAFi8a4F-DmcHdrd62ydbsz9bLebjam3ilGXcp8r0d3B1R3W2s706iPrl3r7o_CSH11rjBTVPWd9_T2QK1-M-p9-9lt-vd_ccv2bblvO6PsWq9WH58LrPb7_Y_7B4mKfpI</recordid><startdate>196704</startdate><enddate>196704</enddate><creator>LOVELACE, C. JAMES</creator><creator>MILLER, GENE W</creator><general>Histochemical Soc</general><general>SAGE Publications</general><scope>AAYXX</scope><scope>CITATION</scope><scope>OTOTI</scope></search><sort><creationdate>196704</creationdate><title>HISTOCHEMICAL INVESTIGATIONS ON THE IN VIVO EFFECTS OF FLUORIDE ON TRICARBOXYLIC ACID CYCLE DEHYDROGENASES FROM PELARGONIUM ZONALE: PART II</title><author>LOVELACE, C. JAMES ; MILLER, GENE W</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c1542-6e2a594487216aada8e45a99781fece62a15b676f90b1c0eaf3da69e47858bfe3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1967</creationdate><topic>560303 - Chemicals Metabolism & Toxicology- Plants- (-1987)</topic><topic>ALKALI METAL COMPOUNDS</topic><topic>CARBOXYLIC ACIDS</topic><topic>ENZYME ACTIVITY</topic><topic>ENZYMES</topic><topic>FLUORIDES</topic><topic>FLUORINE COMPOUNDS</topic><topic>HALIDES</topic><topic>HALOGEN COMPOUNDS</topic><topic>HYDROFLUORIC ACID</topic><topic>HYDROGEN COMPOUNDS</topic><topic>IN VIVO</topic><topic>INJURIES</topic><topic>INORGANIC ACIDS</topic><topic>LEAVES</topic><topic>METABOLISM</topic><topic>ORGANIC ACIDS</topic><topic>ORGANIC COMPOUNDS</topic><topic>ORNAMENTAL PLANTS</topic><topic>OXIDOREDUCTASES</topic><topic>PLANTS</topic><topic>RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT</topic><topic>SODIUM COMPOUNDS</topic><topic>SODIUM FLUORIDES</topic><topic>TOXICITY</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>LOVELACE, C. JAMES</creatorcontrib><creatorcontrib>MILLER, GENE W</creatorcontrib><creatorcontrib>Humboldt State College, Arcata, CA</creatorcontrib><collection>CrossRef</collection><collection>OSTI.GOV</collection><jtitle>J. Histochem. Cytochem.; (United States)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>LOVELACE, C. JAMES</au><au>MILLER, GENE W</au><aucorp>Humboldt State College, Arcata, CA</aucorp><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>HISTOCHEMICAL INVESTIGATIONS ON THE IN VIVO EFFECTS OF FLUORIDE ON TRICARBOXYLIC ACID CYCLE DEHYDROGENASES FROM PELARGONIUM ZONALE: PART II</atitle><jtitle>J. Histochem. Cytochem.; (United States)</jtitle><addtitle>J. Histochem. Cytochem</addtitle><date>1967-04</date><risdate>1967</risdate><volume>15</volume><issue>4</issue><spage>202</spage><epage>206</epage><pages>202-206</pages><issn>0022-1554</issn><eissn>1551-5044</eissn><abstract>In vivo effects of fluoride on tricarboxylic acid (TCA) cycle dehydrogenase enzymes of Pelargonium zonale were studied using p-nitro blue tetrazoleum chloride. Plants were exposed to 17 ppb HF, and enzyme activities in treated plants were compared to those in controls. Leaves of control plants were incubated in 5 x 10–3
M sodium fluoride. Injuries observed in fumigation and solution experiments were similar. Leaf tissue subjected to HF or sodium fluoride evidenced less succinic p-nitro blue tetrazoleum reductase activity than did control tissue. Other TCA cycle dehydrogenase enzymes were not observably affected by the fluoride concentrations used in these experiments. Excised leaves cultured in 5 x 10–3
M sodium fluoride exhibited less succinic p-nitro blue tetrazoleum reductase activity after 24 hr than did leaves cultured in 5 x 10–3
M sodium chloride.</abstract><cop>London, England</cop><pub>Histochemical Soc</pub><pmid>6033028</pmid><doi>10.1177/15.4.202</doi><tpages>5</tpages><oa>free_for_read</oa></addata></record> |
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subjects | 560303 - Chemicals Metabolism & Toxicology- Plants- (-1987) ALKALI METAL COMPOUNDS CARBOXYLIC ACIDS ENZYME ACTIVITY ENZYMES FLUORIDES FLUORINE COMPOUNDS HALIDES HALOGEN COMPOUNDS HYDROFLUORIC ACID HYDROGEN COMPOUNDS IN VIVO INJURIES INORGANIC ACIDS LEAVES METABOLISM ORGANIC ACIDS ORGANIC COMPOUNDS ORNAMENTAL PLANTS OXIDOREDUCTASES PLANTS RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT SODIUM COMPOUNDS SODIUM FLUORIDES TOXICITY |
title | HISTOCHEMICAL INVESTIGATIONS ON THE IN VIVO EFFECTS OF FLUORIDE ON TRICARBOXYLIC ACID CYCLE DEHYDROGENASES FROM PELARGONIUM ZONALE: PART II |
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