METHOD FOR ENHANCEMENT OF ELECTRON MICROSCOPIC VISUALIZATION OF EMBEDDED ANTIGEN BY BRIDGING OSMIUM TO URANIUM ANTIBODY WITH THIOCARBOHYDRAZIDE

Thiocarbohydrazide (TCH), a bidentate ligand, capable of bridging osmium to osmium in ultrathin sections (OTO method), was shown to possess the additional property of binding osmium to uranium-labeled antibody. On sections in which acrolein-fixed antigen had been localized for electron microscopy by...

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Veröffentlicht in:	J. Histochem. Cytochem., 14: 711-18(Oct. 1966) 14: 711-18(Oct. 1966), 1966-10, Vol.14 (10), p.711-718
Hauptverfasser:	STERNBERGER, LUDWIG A, HANKER, JACOB S, DONATI, EDWARD J, PETRALI, JOHN P, SELIGMAN, ARNOLD M
Format:	Artikel
Sprache:	eng
Schlagworte:	ANTIBODIES CHELATES ELECTRON MICROSCOPY IONS N28110 -Life Sciences-Biochemistry, Physiology, & Molecular Biology-Basic Processes PREPARATION SAMPLING TESTING TISSUES URANIUM
Online-Zugang:	Volltext
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