METHOD FOR ENHANCEMENT OF ELECTRON MICROSCOPIC VISUALIZATION OF EMBEDDED ANTIGEN BY BRIDGING OSMIUM TO URANIUM ANTIBODY WITH THIOCARBOHYDRAZIDE

Thiocarbohydrazide (TCH), a bidentate ligand, capable of bridging osmium to osmium in ultrathin sections (OTO method), was shown to possess the additional property of binding osmium to uranium-labeled antibody. On sections in which acrolein-fixed antigen had been localized for electron microscopy by...

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Veröffentlicht in:	J. Histochem. Cytochem., 14: 711-18(Oct. 1966) 14: 711-18(Oct. 1966), 1966-10, Vol.14 (10), p.711-718
Hauptverfasser:	STERNBERGER, LUDWIG A, HANKER, JACOB S, DONATI, EDWARD J, PETRALI, JOHN P, SELIGMAN, ARNOLD M
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Sprache:	eng
Schlagworte:	ANTIBODIES CHELATES ELECTRON MICROSCOPY IONS N28110 -Life Sciences-Biochemistry, Physiology, & Molecular Biology-Basic Processes PREPARATION SAMPLING TESTING TISSUES URANIUM
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container_end_page	718
container_issue	10
container_start_page	711
container_title	J. Histochem. Cytochem., 14: 711-18(Oct. 1966)
container_volume	14
creator	STERNBERGER, LUDWIG A HANKER, JACOB S DONATI, EDWARD J PETRALI, JOHN P SELIGMAN, ARNOLD M
description	Thiocarbohydrazide (TCH), a bidentate ligand, capable of bridging osmium to osmium in ultrathin sections (OTO method), was shown to possess the additional property of binding osmium to uranium-labeled antibody. On sections in which acrolein-fixed antigen had been localized for electron microscopy by uranium antibody, application of TCH followed by osmication (T-O procedure) greatly enhanced the specific contrast afforded. In addition, the T-O procedure yielded better resolution and better stability to the electron beam than the immunouranium technique employed without this intensification. Even osmium tetroxide-fixed tissue could be used, providing thiosemicarbazide (TSC) was employed to block the later reaction of TCH with tissue-bound osmium and to limit, thereby, the ligation of TCH to the uranium-labeled antibody.
doi_str_mv	10.1177/14.10.711
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Histochem. Cytochem., 14: 711-18(Oct. 1966)</title><addtitle>J. Histochem. Cytochem</addtitle><description>Thiocarbohydrazide (TCH), a bidentate ligand, capable of bridging osmium to osmium in ultrathin sections (OTO method), was shown to possess the additional property of binding osmium to uranium-labeled antibody. On sections in which acrolein-fixed antigen had been localized for electron microscopy by uranium antibody, application of TCH followed by osmication (T-O procedure) greatly enhanced the specific contrast afforded. In addition, the T-O procedure yielded better resolution and better stability to the electron beam than the immunouranium technique employed without this intensification. Even osmium tetroxide-fixed tissue could be used, providing thiosemicarbazide (TSC) was employed to block the later reaction of TCH with tissue-bound osmium and to limit, thereby, the ligation of TCH to the uranium-labeled antibody.</description><subject>ANTIBODIES</subject><subject>CHELATES</subject><subject>ELECTRON MICROSCOPY</subject><subject>IONS</subject><subject>N28110 -Life Sciences-Biochemistry, Physiology, & Molecular Biology-Basic Processes</subject><subject>PREPARATION</subject><subject>SAMPLING</subject><subject>TESTING</subject><subject>TISSUES</subject><subject>URANIUM</subject><issn>0022-1554</issn><issn>1551-5044</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1966</creationdate><recordtype>article</recordtype><recordid>eNp90M1O4zAQB3BrtQi67B54A4vDShwCtuN89JgPt7HUxCh1F5WLlTouDSotiouqfQpeGUdF4sZp_pr5aTQaAK4wusU4iu4wvXUxwvgHGOEgwF6AKP0JRggR4rkGvQC_rH1GCFMaxOfgPBiH4ygIR-C9ZLIQOZyIGrKqSKqMlaySUEwgm7FM1qKCJc9qMc_EPc_gPz5fJDP-mEjuJoMqU5bnLIdJJfmUVTBdwrTm-ZRXUyjmJV-UUAq4qJNqiINKRb6ED1wWUBZcZEmdimKZ18kjz9lvcLZuttb8-ayXYDFhMiu8mZjyLJl5msT-wVtF7Sr0_Yi0Tdi2Pg11iwhd08BEJg5cz2-0JpiEJI5Ni8Zk1TYYUaxbYyI39S_B9Wnv3h46ZXV3MHqj97ud0QdFKRljP3To5oR0v7e2N2v12ncvTf9fYaSGxytMh-ge7-zfk7XNk1HP-7d-5-7_Dm66p82x642yL812-_q2wup4PH7BD_VugRQ</recordid><startdate>196610</startdate><enddate>196610</enddate><creator>STERNBERGER, LUDWIG A</creator><creator>HANKER, JACOB S</creator><creator>DONATI, EDWARD J</creator><creator>PETRALI, JOHN P</creator><creator>SELIGMAN, ARNOLD M</creator><general>Histochemical Soc</general><general>SAGE Publications</general><scope>AAYXX</scope><scope>CITATION</scope><scope>OTOTI</scope></search><sort><creationdate>196610</creationdate><title>METHOD FOR ENHANCEMENT OF ELECTRON MICROSCOPIC VISUALIZATION OF EMBEDDED ANTIGEN BY BRIDGING OSMIUM TO URANIUM ANTIBODY WITH THIOCARBOHYDRAZIDE</title><author>STERNBERGER, LUDWIG A ; HANKER, JACOB S ; DONATI, EDWARD J ; PETRALI, JOHN P ; SELIGMAN, ARNOLD M</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c283t-b7db63372da6dd346cd024f45e7e85a6d3acc2126288ed092bda1041cdee76d33</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1966</creationdate><topic>ANTIBODIES</topic><topic>CHELATES</topic><topic>ELECTRON MICROSCOPY</topic><topic>IONS</topic><topic>N28110 -Life Sciences-Biochemistry, Physiology, & Molecular Biology-Basic Processes</topic><topic>PREPARATION</topic><topic>SAMPLING</topic><topic>TESTING</topic><topic>TISSUES</topic><topic>URANIUM</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>STERNBERGER, LUDWIG A</creatorcontrib><creatorcontrib>HANKER, JACOB S</creatorcontrib><creatorcontrib>DONATI, EDWARD J</creatorcontrib><creatorcontrib>PETRALI, JOHN P</creatorcontrib><creatorcontrib>SELIGMAN, ARNOLD M</creatorcontrib><creatorcontrib>Medical Research Labs., Edgewood Arsenal, Md</creatorcontrib><collection>CrossRef</collection><collection>OSTI.GOV</collection><jtitle>J. Histochem. Cytochem., 14: 711-18(Oct. 1966)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>STERNBERGER, LUDWIG A</au><au>HANKER, JACOB S</au><au>DONATI, EDWARD J</au><au>PETRALI, JOHN P</au><au>SELIGMAN, ARNOLD M</au><aucorp>Medical Research Labs., Edgewood Arsenal, Md</aucorp><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>METHOD FOR ENHANCEMENT OF ELECTRON MICROSCOPIC VISUALIZATION OF EMBEDDED ANTIGEN BY BRIDGING OSMIUM TO URANIUM ANTIBODY WITH THIOCARBOHYDRAZIDE</atitle><jtitle>J. Histochem. Cytochem., 14: 711-18(Oct. 1966)</jtitle><addtitle>J. Histochem. Cytochem</addtitle><date>1966-10</date><risdate>1966</risdate><volume>14</volume><issue>10</issue><spage>711</spage><epage>718</epage><pages>711-718</pages><issn>0022-1554</issn><eissn>1551-5044</eissn><abstract>Thiocarbohydrazide (TCH), a bidentate ligand, capable of bridging osmium to osmium in ultrathin sections (OTO method), was shown to possess the additional property of binding osmium to uranium-labeled antibody. On sections in which acrolein-fixed antigen had been localized for electron microscopy by uranium antibody, application of TCH followed by osmication (T-O procedure) greatly enhanced the specific contrast afforded. In addition, the T-O procedure yielded better resolution and better stability to the electron beam than the immunouranium technique employed without this intensification. 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subjects	ANTIBODIES CHELATES ELECTRON MICROSCOPY IONS N28110 -Life Sciences-Biochemistry, Physiology, & Molecular Biology-Basic Processes PREPARATION SAMPLING TESTING TISSUES URANIUM
title	METHOD FOR ENHANCEMENT OF ELECTRON MICROSCOPIC VISUALIZATION OF EMBEDDED ANTIGEN BY BRIDGING OSMIUM TO URANIUM ANTIBODY WITH THIOCARBOHYDRAZIDE
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