THE INFLUENCE OF TECHNIQUE AND HORMONES ON HISTOCHEMICAL DEMONSTRATION OF ENZYMES IN INTESTINAL EPITHELIUM
The influence of technical variations and of hypophysectomy and adrenalectomy on the demonstration of enzymes in the jejunal epithelium was studied. The application to fresh frozen sections of procedures for cytochrome c oxidase, succinic dehydrogenase, diphosphopyridine nucleotide diaphorase and la...
Gespeichert in:
| Veröffentlicht in: | The journal of histochemistry and cytochemistry 1963-05, Vol.11 (3), p.349-364 |
|---|---|
| Hauptverfasser: | , |
| Format: | Artikel |
| Sprache: | eng |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 364 |
|---|---|
| container_issue | 3 |
| container_start_page | 349 |
| container_title | The journal of histochemistry and cytochemistry |
| container_volume | 11 |
| creator | LIU, HSI-YEN BAKER, BURTON L |
| description | The influence of technical variations and of hypophysectomy and adrenalectomy on the demonstration of enzymes in the jejunal epithelium was studied. The application to fresh frozen sections of procedures for cytochrome c oxidase, succinic dehydrogenase, diphosphopyridine nucleotide diaphorase and lactic dehydrogenase failed to reveal a precise mitochondrial localization, basing the conclusion on comparison with mitochondria as demonstrated by staining of Regaud-fixed mucosa with the Altmann-Masson procedure. Fixation in cold formalin prior to freezing and sectioning permitted a more satisfactory localization of succinic dehydrogenase in mitochondria.
Hypophysectomy or adrenalectomy resulted in a reduction in number, size, staining and orderly arrangement of mitochondria in the villous epithelium as revealed in cytological preparations and those fixed and stained for succinic dehydrogenase. Alkaline phosphatase activity was also depressed. The intensification of cytoplasmic staining for lactic dehydrogenase, diphosphopyridine nucleotide diaphorase and succinic dehydrogenase in fresh frozen tissue probably represents concentration of formazan by reduction in cell volume and not increased enzyme activities. |
| doi_str_mv | 10.1177/11.3.349 |
| format | Article |
| fullrecord | <record><control><sourceid>sage_cross</sourceid><recordid>TN_cdi_crossref_primary_10_1177_11_3_349</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sage_id>10.1177_11.3.349</sage_id><sourcerecordid>10.1177_11.3.349</sourcerecordid><originalsourceid>FETCH-LOGICAL-c217t-5f4d8fb5dd8f586d68a259156ed5af6a45a712e51d3658c41828b1203e8ccb163</originalsourceid><addsrcrecordid>eNp9kE1Lw0AQhhdRsFbBn7AXwUvqTrKbpMeSbs1CstFmc9DLsvnqB62VxBL8926peBSGGZh55p3hRegeyAQgCJ4AJt7Eo9MLNALGwGGE0ks0IsR1Hdug1-im77eEAKUsHKGtijkWcpEUXEYcZwuseBRL8VpwPJNzHGfLNJM8x5nEschVFsU8FdEswXNuB7lazpSwM7vI5ftbakkhbSieKyEtxl-EPZGIIr1FV63Z9c3dbx2jYsFVFDtJ9nxSdCoXgi-HtbQO25LVNrPQr_3QuGwKzG9qZlrfUGYCcBsGteezsKIQumEJLvGasKpK8L0xejzrVt2h77um1Z_dZm-6bw1EnzyySXvaemTRhzPam1Wjt4dj92E_-4dbb1brYdM1ut-b3e7zWIIehuGP-wHSJWhZ</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype></control><display><type>article</type><title>THE INFLUENCE OF TECHNIQUE AND HORMONES ON HISTOCHEMICAL DEMONSTRATION OF ENZYMES IN INTESTINAL EPITHELIUM</title><source>SAGE Complete A-Z List</source><source>Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals</source><source>Free Full-Text Journals in Chemistry</source><creator>LIU, HSI-YEN ; BAKER, BURTON L</creator><creatorcontrib>LIU, HSI-YEN ; BAKER, BURTON L</creatorcontrib><description>The influence of technical variations and of hypophysectomy and adrenalectomy on the demonstration of enzymes in the jejunal epithelium was studied. The application to fresh frozen sections of procedures for cytochrome c oxidase, succinic dehydrogenase, diphosphopyridine nucleotide diaphorase and lactic dehydrogenase failed to reveal a precise mitochondrial localization, basing the conclusion on comparison with mitochondria as demonstrated by staining of Regaud-fixed mucosa with the Altmann-Masson procedure. Fixation in cold formalin prior to freezing and sectioning permitted a more satisfactory localization of succinic dehydrogenase in mitochondria.
Hypophysectomy or adrenalectomy resulted in a reduction in number, size, staining and orderly arrangement of mitochondria in the villous epithelium as revealed in cytological preparations and those fixed and stained for succinic dehydrogenase. Alkaline phosphatase activity was also depressed. The intensification of cytoplasmic staining for lactic dehydrogenase, diphosphopyridine nucleotide diaphorase and succinic dehydrogenase in fresh frozen tissue probably represents concentration of formazan by reduction in cell volume and not increased enzyme activities.</description><identifier>ISSN: 0022-1554</identifier><identifier>EISSN: 1551-5044</identifier><identifier>DOI: 10.1177/11.3.349</identifier><language>eng</language><publisher>London, England: Histochemical Soc</publisher><ispartof>The journal of histochemistry and cytochemistry, 1963-05, Vol.11 (3), p.349-364</ispartof><rights>1963, THE WILLIAMS & WILKINS COMPANY</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c217t-5f4d8fb5dd8f586d68a259156ed5af6a45a712e51d3658c41828b1203e8ccb163</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://journals.sagepub.com/doi/pdf/10.1177/11.3.349$$EPDF$$P50$$Gsage$$H</linktopdf><linktohtml>$$Uhttps://journals.sagepub.com/doi/10.1177/11.3.349$$EHTML$$P50$$Gsage$$H</linktohtml><link.rule.ids>314,776,780,21798,27901,27902,43597,43598</link.rule.ids></links><search><creatorcontrib>LIU, HSI-YEN</creatorcontrib><creatorcontrib>BAKER, BURTON L</creatorcontrib><title>THE INFLUENCE OF TECHNIQUE AND HORMONES ON HISTOCHEMICAL DEMONSTRATION OF ENZYMES IN INTESTINAL EPITHELIUM</title><title>The journal of histochemistry and cytochemistry</title><addtitle>J. Histochem. Cytochem</addtitle><description>The influence of technical variations and of hypophysectomy and adrenalectomy on the demonstration of enzymes in the jejunal epithelium was studied. The application to fresh frozen sections of procedures for cytochrome c oxidase, succinic dehydrogenase, diphosphopyridine nucleotide diaphorase and lactic dehydrogenase failed to reveal a precise mitochondrial localization, basing the conclusion on comparison with mitochondria as demonstrated by staining of Regaud-fixed mucosa with the Altmann-Masson procedure. Fixation in cold formalin prior to freezing and sectioning permitted a more satisfactory localization of succinic dehydrogenase in mitochondria.
Hypophysectomy or adrenalectomy resulted in a reduction in number, size, staining and orderly arrangement of mitochondria in the villous epithelium as revealed in cytological preparations and those fixed and stained for succinic dehydrogenase. Alkaline phosphatase activity was also depressed. The intensification of cytoplasmic staining for lactic dehydrogenase, diphosphopyridine nucleotide diaphorase and succinic dehydrogenase in fresh frozen tissue probably represents concentration of formazan by reduction in cell volume and not increased enzyme activities.</description><issn>0022-1554</issn><issn>1551-5044</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1963</creationdate><recordtype>article</recordtype><recordid>eNp9kE1Lw0AQhhdRsFbBn7AXwUvqTrKbpMeSbs1CstFmc9DLsvnqB62VxBL8926peBSGGZh55p3hRegeyAQgCJ4AJt7Eo9MLNALGwGGE0ks0IsR1Hdug1-im77eEAKUsHKGtijkWcpEUXEYcZwuseBRL8VpwPJNzHGfLNJM8x5nEschVFsU8FdEswXNuB7lazpSwM7vI5ftbakkhbSieKyEtxl-EPZGIIr1FV63Z9c3dbx2jYsFVFDtJ9nxSdCoXgi-HtbQO25LVNrPQr_3QuGwKzG9qZlrfUGYCcBsGteezsKIQumEJLvGasKpK8L0xejzrVt2h77um1Z_dZm-6bw1EnzyySXvaemTRhzPam1Wjt4dj92E_-4dbb1brYdM1ut-b3e7zWIIehuGP-wHSJWhZ</recordid><startdate>196305</startdate><enddate>196305</enddate><creator>LIU, HSI-YEN</creator><creator>BAKER, BURTON L</creator><general>Histochemical Soc</general><general>SAGE Publications</general><scope>AAYXX</scope><scope>CITATION</scope></search><sort><creationdate>196305</creationdate><title>THE INFLUENCE OF TECHNIQUE AND HORMONES ON HISTOCHEMICAL DEMONSTRATION OF ENZYMES IN INTESTINAL EPITHELIUM</title><author>LIU, HSI-YEN ; BAKER, BURTON L</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c217t-5f4d8fb5dd8f586d68a259156ed5af6a45a712e51d3658c41828b1203e8ccb163</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1963</creationdate><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>LIU, HSI-YEN</creatorcontrib><creatorcontrib>BAKER, BURTON L</creatorcontrib><collection>CrossRef</collection><jtitle>The journal of histochemistry and cytochemistry</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>LIU, HSI-YEN</au><au>BAKER, BURTON L</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>THE INFLUENCE OF TECHNIQUE AND HORMONES ON HISTOCHEMICAL DEMONSTRATION OF ENZYMES IN INTESTINAL EPITHELIUM</atitle><jtitle>The journal of histochemistry and cytochemistry</jtitle><addtitle>J. Histochem. Cytochem</addtitle><date>1963-05</date><risdate>1963</risdate><volume>11</volume><issue>3</issue><spage>349</spage><epage>364</epage><pages>349-364</pages><issn>0022-1554</issn><eissn>1551-5044</eissn><abstract>The influence of technical variations and of hypophysectomy and adrenalectomy on the demonstration of enzymes in the jejunal epithelium was studied. The application to fresh frozen sections of procedures for cytochrome c oxidase, succinic dehydrogenase, diphosphopyridine nucleotide diaphorase and lactic dehydrogenase failed to reveal a precise mitochondrial localization, basing the conclusion on comparison with mitochondria as demonstrated by staining of Regaud-fixed mucosa with the Altmann-Masson procedure. Fixation in cold formalin prior to freezing and sectioning permitted a more satisfactory localization of succinic dehydrogenase in mitochondria.
Hypophysectomy or adrenalectomy resulted in a reduction in number, size, staining and orderly arrangement of mitochondria in the villous epithelium as revealed in cytological preparations and those fixed and stained for succinic dehydrogenase. Alkaline phosphatase activity was also depressed. The intensification of cytoplasmic staining for lactic dehydrogenase, diphosphopyridine nucleotide diaphorase and succinic dehydrogenase in fresh frozen tissue probably represents concentration of formazan by reduction in cell volume and not increased enzyme activities.</abstract><cop>London, England</cop><pub>Histochemical Soc</pub><doi>10.1177/11.3.349</doi><tpages>16</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0022-1554 |
| ispartof | The journal of histochemistry and cytochemistry, 1963-05, Vol.11 (3), p.349-364 |
| issn | 0022-1554 1551-5044 |
| language | eng |
| recordid | cdi_crossref_primary_10_1177_11_3_349 |
| source | SAGE Complete A-Z List; Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals; Free Full-Text Journals in Chemistry |
| title | THE INFLUENCE OF TECHNIQUE AND HORMONES ON HISTOCHEMICAL DEMONSTRATION OF ENZYMES IN INTESTINAL EPITHELIUM |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-02-05T04%3A11%3A16IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-sage_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=THE%20INFLUENCE%20OF%20TECHNIQUE%20AND%20HORMONES%20ON%20HISTOCHEMICAL%20DEMONSTRATION%20OF%20ENZYMES%20IN%20INTESTINAL%20EPITHELIUM&rft.jtitle=The%20journal%20of%20histochemistry%20and%20cytochemistry&rft.au=LIU,%20HSI-YEN&rft.date=1963-05&rft.volume=11&rft.issue=3&rft.spage=349&rft.epage=364&rft.pages=349-364&rft.issn=0022-1554&rft.eissn=1551-5044&rft_id=info:doi/10.1177/11.3.349&rft_dat=%3Csage_cross%3E10.1177_11.3.349%3C/sage_cross%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_id=info:pmid/&rft_sage_id=10.1177_11.3.349&rfr_iscdi=true |