High-Throughput Screen for Escherichia coli Heat Shock Protein 70 (Hsp70/DnaK): ATPase Assay in Low Volume by Exploiting Energy Transfer

Members of the heat shock protein 70 (Hsp70) family of molecular chaperones are emerging as potential therapeutic targets. Their ATPase activity has classically been measured using colorimetric phosphate detection reagents, such as quinaldine red (QR). Although such assays are suitable for 96-well p...

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Veröffentlicht in:	Journal of biomolecular screening 2010-12, Vol.15 (10), p.1211-1219
Hauptverfasser:	Miyata, Yoshinari, Chang, Lyra, Bainor, Anthony, Mcquade, Thomas J., Walczak, Christopher P., Zhang, Yaru, Larsen, Martha J., Kirchhoff, Paul, Gestwicki, Jason E.
Format:	Artikel
Sprache:	eng
Schlagworte:	Adenosine Triphosphatases - metabolism ATPase Drug Discovery Escherichia coli Escherichia coli Proteins - antagonists & inhibitors Escherichia coli Proteins - metabolism fluorescence assay Fluorescence Resonance Energy Transfer High-Throughput Screening Assays - methods HSP40 Heat-Shock Proteins - antagonists & inhibitors HSP40 Heat-Shock Proteins - metabolism HSP70 Heat-Shock Proteins - antagonists & inhibitors HSP70 Heat-Shock Proteins - metabolism malachite green molecular chaperone Molecular Chaperones - antagonists & inhibitors Molecular Chaperones - metabolism phosphate Small Molecule Libraries
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