Buffer Optimization of Thermal Melt Assays of Plasmodium Proteins for Detection of Small-Molecule Ligands

In the past decade, thermal melt/thermal shift assays have become a common tool for identifying ligands and other factors that stabilize specific proteins. Increased stability is indicated by an increase in the protein’s melting temperature (Tm). In optimizing the assays for subsequent screening of...

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Veröffentlicht in:	Journal of biomolecular screening 2009-07, Vol.14 (6), p.700-707
Hauptverfasser:	Crowther, Gregory J., Napuli, Alberto J., Thomas, Andrew P., Chung, Diana J., Kovzun, Kuzma V., Leibly, David J., Castaneda, Lisa J., Bhandari, Janhavi, Damman, Christopher J., Hui, Raymond, Hol, Wim G.J., Buckner, Frederick S., Verlinde, Christophe L.M.J., Zhang, Zhongsheng, Fan, Erkang, Van Voorhis, Wesley C.
Format:	Artikel
Sprache:	eng
Schlagworte:	Animals Biological Assay - methods Buffers Ligands Plasmodium Plasmodium - chemistry protein stabilization protein unfolding Protozoan Proteins - chemistry Small Molecule Libraries - analysis superoxide dismutase thermal shift assays Transition Temperature
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