Use of Cryopreserved Cells for Enabling Greater Flexibility in Compound Profiling
Measurement of intracellular calcium release following agonist challenge within cells expressing the relevant membrane protein is a commonly used format to derive structure-activity relationship (SAR) data within a compound profiling assay. The Fluorometric Imaging Plate Reader (FLIPR) has become th...
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| Veröffentlicht in: | Journal of biomolecular screening 2008-06, Vol.13 (5), p.354-362 |
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| creator | Wigglesworth, M.J. Lawless, K.J. Standing, D.J. Mackenzie, E.K. Kitchen, V.R. Mckay, F. Ward, E. Brough, S.J. Stylianou, M. Jewitt, F.R. Mclaren-Douglas, A.M. Jowet, M.I. Tamayama, N. Finnigan, D. Ding, J. Wise, A. |
| description | Measurement of intracellular calcium release following agonist challenge within cells expressing the relevant membrane protein is a commonly used format to derive structure-activity relationship (SAR) data within a compound profiling assay. The Fluorometric Imaging Plate Reader (FLIPR) has become the gold standard for this purpose. FLIPR traditionally uses cells that are maintained in continuous culture for compound profiling of iterative chemistry campaigns. This supply dictates that assays can only be run on 4 of 5 weekdays, or alternative cell culture machinery is required such that plating can occur remotely at the weekend. The data reported here demonstrate that high-quality compound profiling data can be generated from the use of cryopreserved cells and that these cells can also be plated at various densities to generate equivalent data between 24 and 72 h post-plating. Hence, the authors report a method that allows data generation throughout the week and without the requirement of highly automated cell culture or continuous culture. (Journal of Biomolecular Screening 2008:354-362) |
| doi_str_mv | 10.1177/1087057108317768 |
| format | Article |
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The Fluorometric Imaging Plate Reader (FLIPR) has become the gold standard for this purpose. FLIPR traditionally uses cells that are maintained in continuous culture for compound profiling of iterative chemistry campaigns. This supply dictates that assays can only be run on 4 of 5 weekdays, or alternative cell culture machinery is required such that plating can occur remotely at the weekend. The data reported here demonstrate that high-quality compound profiling data can be generated from the use of cryopreserved cells and that these cells can also be plated at various densities to generate equivalent data between 24 and 72 h post-plating. Hence, the authors report a method that allows data generation throughout the week and without the requirement of highly automated cell culture or continuous culture. 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The Fluorometric Imaging Plate Reader (FLIPR) has become the gold standard for this purpose. FLIPR traditionally uses cells that are maintained in continuous culture for compound profiling of iterative chemistry campaigns. This supply dictates that assays can only be run on 4 of 5 weekdays, or alternative cell culture machinery is required such that plating can occur remotely at the weekend. The data reported here demonstrate that high-quality compound profiling data can be generated from the use of cryopreserved cells and that these cells can also be plated at various densities to generate equivalent data between 24 and 72 h post-plating. Hence, the authors report a method that allows data generation throughout the week and without the requirement of highly automated cell culture or continuous culture. (Journal of Biomolecular Screening 2008:354-362)</description><subject>Animals</subject><subject>Calcium - analysis</subject><subject>Calcium - metabolism</subject><subject>Cells</subject><subject>CHO Cells</subject><subject>Cricetinae</subject><subject>Cricetulus</subject><subject>Cryopreservation</subject><subject>Cryopreservation of organs, tissues, etc</subject><subject>Drugs</subject><subject>Fluorimetry</subject><subject>Fluorometry - instrumentation</subject><subject>Fluorometry - methods</subject><subject>Histamine</subject><subject>Humans</subject><subject>Identification and classification</subject><subject>Methods</subject><subject>Physiological aspects</subject><subject>Product/Service Evaluations</subject><subject>Structure-Activity Relationship</subject><subject>Structure-activity relationship (Pharmacology)</subject><subject>Structure-activity relationships</subject><issn>1087-0571</issn><issn>2472-5552</issn><issn>1552-454X</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2008</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqNkc9r2zAUx8XYaLus952KTr05k2RZP47BpF0hsBYW2E3I8nNQsa1Mskfz308hgUJhdOjw9Hif75ev9BD6SsmSUim_UaIkqWQuZW6F-oCuaFWxglf818d8z-PiOL9En1N6JoSWgvALdEkVF1IIfYWetglw6HAdD2EfIUH8Ay2uoe8T7kLE69E2vR93-D6CnSDiux5efON7Px2wH3Edhn2YxxY_xtD5I_kFfepsn-D6XBdoe7f-WX8vNj_uH-rVpnBcialoy0ZyaXWjG-Vo55xj1pUup6qEhI44Jq0TshWWUiqIqqQC2lS2bEWlgalygW5PvvsYfs-QJjP45HJwO0KYkxGacUZK8i7IKCOM0KPj8gTubA_Gj12YonX5tDB4F0bIDwSzoopwxbVm_y2QWmSc6SwgJ4GLIaUIndlHP9h4MJSY40bN241myc05_dwM0L4KzivMQHECkt2BeQ5zHPO3_9vwL31fpyg</recordid><startdate>20080601</startdate><enddate>20080601</enddate><creator>Wigglesworth, M.J.</creator><creator>Lawless, K.J.</creator><creator>Standing, D.J.</creator><creator>Mackenzie, E.K.</creator><creator>Kitchen, V.R.</creator><creator>Mckay, F.</creator><creator>Ward, E.</creator><creator>Brough, S.J.</creator><creator>Stylianou, M.</creator><creator>Jewitt, F.R.</creator><creator>Mclaren-Douglas, A.M.</creator><creator>Jowet, M.I.</creator><creator>Tamayama, N.</creator><creator>Finnigan, D.</creator><creator>Ding, J.</creator><creator>Wise, A.</creator><general>SAGE Publications</general><general>Sage Publications, Inc</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QO</scope><scope>8FD</scope><scope>FR3</scope><scope>P64</scope><scope>7X8</scope></search><sort><creationdate>20080601</creationdate><title>Use of Cryopreserved Cells for Enabling Greater Flexibility in Compound Profiling</title><author>Wigglesworth, M.J. ; 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| ispartof | Journal of biomolecular screening, 2008-06, Vol.13 (5), p.354-362 |
| issn | 1087-0571 2472-5552 1552-454X |
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| source | MEDLINE; Alma/SFX Local Collection |
| subjects | Animals Calcium - analysis Calcium - metabolism Cells CHO Cells Cricetinae Cricetulus Cryopreservation Cryopreservation of organs, tissues, etc Drugs Fluorimetry Fluorometry - instrumentation Fluorometry - methods Histamine Humans Identification and classification Methods Physiological aspects Product/Service Evaluations Structure-Activity Relationship Structure-activity relationship (Pharmacology) Structure-activity relationships |
| title | Use of Cryopreserved Cells for Enabling Greater Flexibility in Compound Profiling |
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