A Simple, Flexible, Nonfluorescent System for the Automated Screening of Neurite Outgrowth

Measurement of neurite outgrowth is a common assay of neurotrophic activity. However, currently available techniques for measuring neurite outgrowth are either time or resource intensive. The authors established a system in which chronic treatment of a subcloned SH-SY5Y cell line with aphidicolin an...

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Veröffentlicht in:	Journal of biomolecular screening 2006-03, Vol.11 (2), p.155-164
Hauptverfasser:	Price, Raymond D., Oe, Tomoya, Yamaji, Takayuki, Matsuoka, Nobuya
Format:	Artikel
Sprache:	eng
Schlagworte:	Analysis Aphidicolin - pharmacology Brain-Derived Neurotrophic Factor - pharmacology Cell Count Cell Differentiation - drug effects Cell Line, Tumor Cell Proliferation - drug effects Clone Cells Drug Synergism Humans Imaging systems Nerve growth factor Nerve Growth Factor - pharmacology Neurites - chemistry Neurites - ultrastructure Neuroblastoma rho GTP-Binding Proteins - antagonists & inhibitors Tretinoin - pharmacology
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