Label-Free Assays on the BIND System
Screening of biochemical interactions becomes simpler, less expensive, and more accurate when labels, such as fluorescent dyes, radioactive markers, and colorimetric reactions, are not required to quantify detected material. SRU Biosystems has developed a biosensor technology that is manufactured on...
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| Veröffentlicht in: | Journal of biomolecular screening 2004-09, Vol.9 (6), p.481-490 |
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| container_end_page | 490 |
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| container_issue | 6 |
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| container_title | Journal of biomolecular screening |
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| creator | Cunningham, Brian T. Li, Peter Schulz, Stephen Lin, Bo Baird, Cheryl Gerstenmaier, John Genick, Christine Wang, Frank Fine, Eric Laing, Lance |
| description | Screening of biochemical interactions becomes simpler, less expensive, and more accurate when labels, such as fluorescent dyes, radioactive markers, and colorimetric reactions, are not required to quantify detected material. SRU Biosystems has developed a biosensor technology that is manufactured on continuous sheets of plastic film and incorporated into standard microplates and microarray slides to enable label-free assays to be performed with high throughput, high sensitivity, and low cost per assay. The biosensor incorporates a narrow band guided-mode resonance reflectance filter, in which the reflected color is modulated by the attachment/detachment of biochemical material to the surface. The technology offers 4 orders of linear dynamic range and uniformity within a plate, with a coefficient of variation of 2.5%. Using conventional biochemical immobilization surface chemistries, a wide range of assay applications are enabled. Small molecule screening, cell proliferation/cytotoxicity, enzyme activity screening, protein-protein interaction, and cell membrane receptor expression are among the applications demonstrated. |
| doi_str_mv | 10.1177/1087057104267604 |
| format | Article |
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SRU Biosystems has developed a biosensor technology that is manufactured on continuous sheets of plastic film and incorporated into standard microplates and microarray slides to enable label-free assays to be performed with high throughput, high sensitivity, and low cost per assay. The biosensor incorporates a narrow band guided-mode resonance reflectance filter, in which the reflected color is modulated by the attachment/detachment of biochemical material to the surface. The technology offers 4 orders of linear dynamic range and uniformity within a plate, with a coefficient of variation of 2.5%. Using conventional biochemical immobilization surface chemistries, a wide range of assay applications are enabled. 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| fulltext | fulltext |
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| ispartof | Journal of biomolecular screening, 2004-09, Vol.9 (6), p.481-490 |
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| language | eng |
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| source | MEDLINE; Alma/SFX Local Collection |
| subjects | Animals Biological Assay - methods Biosensing Techniques - instrumentation Biosensing Techniques - methods CHO Cells Cricetinae Cricetulus Enzymes, Immobilized - metabolism Equipment Design Humans Immunoglobulin G - analysis Jurkat Cells Kinetics Protein Interaction Mapping Regression Analysis Sensitivity and Specificity Surface Properties |
| title | Label-Free Assays on the BIND System |
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