Identification of Photoreceptor Precursors in the Pars Plana during Ocular Development and after Retinal Injury

To study the distribution and differentiation of photoreceptor precursors in the ciliary epithelium in mice. Proliferating cells in flat-mount specimens of the ciliary body and retina were studied by bromodeoxyuridine (BRDU; 150 mg/kg) labeling in young C57Bl mice. Immunoreactivity to anti-recoverin...

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Veröffentlicht in:Investigative ophthalmology & visual science 2008-01, Vol.49 (1), p.422-428
Hauptverfasser: Nishiguchi, Koji M, Kaneko, Hiroki, Nakamura, Makoto, Kachi, Shu, Terasaki, Hiroko
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creator Nishiguchi, Koji M
Kaneko, Hiroki
Nakamura, Makoto
Kachi, Shu
Terasaki, Hiroko
description To study the distribution and differentiation of photoreceptor precursors in the ciliary epithelium in mice. Proliferating cells in flat-mount specimens of the ciliary body and retina were studied by bromodeoxyuridine (BRDU; 150 mg/kg) labeling in young C57Bl mice. Immunoreactivity to anti-recoverin, rhodopsin, and Pax6 antibodies and binding to peanut agglutinin were analyzed histologically to assess the distribution and differentiation of photoreceptor progenitors or precursors. Mice injected intraperitoneally with N-methyl-N-nitrosourea (MNU; 60 mg/kg) were also examined. Part of the neuroblast layer composed of BrdU-positive retinal progenitor cells was identified within the ciliary epithelium of the pars plana in continuation of the layer of the peripheral retina during ocular development. In both the ciliary epithelium and the retina, the layer size decreased rapidly and disappeared mostly by postnatal day (P)9. Within the ciliary epithelium of the pars plana, numerous postmitotic rod and cone photoreceptor precursors were identified that rapidly differentiated morphologically and decreased in number with ocular development. Rod precursors were no longer seen in the pars plana at P12, whereas rare presumptive cone precursors persisted even at P120. An increase in the number of presumptive cone precursors (approximately 16-fold) was identified in the pars plana of adult mice with MNU-induced photoreceptor degeneration. Rod and cone precursors were identified in the ciliary epithelium of the murine pars plana during ocular development but nearly disappeared after the completion of histogenesis. However, in response to retinal injury, an increased number of presumptive cone precursors was found even in the adult pars plana.
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Within the ciliary epithelium of the pars plana, numerous postmitotic rod and cone photoreceptor precursors were identified that rapidly differentiated morphologically and decreased in number with ocular development. Rod precursors were no longer seen in the pars plana at P12, whereas rare presumptive cone precursors persisted even at P120. An increase in the number of presumptive cone precursors (approximately 16-fold) was identified in the pars plana of adult mice with MNU-induced photoreceptor degeneration. Rod and cone precursors were identified in the ciliary epithelium of the murine pars plana during ocular development but nearly disappeared after the completion of histogenesis. 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Visual pathways and centers. Vision</topic><topic>Eye Proteins - metabolism</topic><topic>Fluorescent Antibody Technique, Indirect</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Homeodomain Proteins - metabolism</topic><topic>Medical sciences</topic><topic>Methylnitrosourea - toxicity</topic><topic>Mice</topic><topic>Mice, Inbred C57BL</topic><topic>Microscopy, Confocal</topic><topic>Ophthalmology</topic><topic>Paired Box Transcription Factors - metabolism</topic><topic>PAX6 Transcription Factor</topic><topic>Photoreceptor Cells, Vertebrate - cytology</topic><topic>Photoreceptor Cells, Vertebrate - metabolism</topic><topic>Pigment Epithelium of Eye - cytology</topic><topic>Recoverin - metabolism</topic><topic>Repressor Proteins - metabolism</topic><topic>Retinal Degeneration - chemically induced</topic><topic>Retinal Degeneration - pathology</topic><topic>Rhodopsin - metabolism</topic><topic>Stem Cells - cytology</topic><topic>Stem Cells - metabolism</topic><topic>Vertebrates: nervous system and sense organs</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Nishiguchi, Koji M</creatorcontrib><creatorcontrib>Kaneko, Hiroki</creatorcontrib><creatorcontrib>Nakamura, Makoto</creatorcontrib><creatorcontrib>Kachi, Shu</creatorcontrib><creatorcontrib>Terasaki, Hiroko</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><jtitle>Investigative ophthalmology &amp; visual science</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Nishiguchi, Koji M</au><au>Kaneko, Hiroki</au><au>Nakamura, Makoto</au><au>Kachi, Shu</au><au>Terasaki, Hiroko</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Identification of Photoreceptor Precursors in the Pars Plana during Ocular Development and after Retinal Injury</atitle><jtitle>Investigative ophthalmology &amp; visual science</jtitle><addtitle>Invest Ophthalmol Vis Sci</addtitle><date>2008-01-01</date><risdate>2008</risdate><volume>49</volume><issue>1</issue><spage>422</spage><epage>428</epage><pages>422-428</pages><issn>0146-0404</issn><issn>1552-5783</issn><eissn>1552-5783</eissn><coden>IOVSDA</coden><abstract>To study the distribution and differentiation of photoreceptor precursors in the ciliary epithelium in mice. Proliferating cells in flat-mount specimens of the ciliary body and retina were studied by bromodeoxyuridine (BRDU; 150 mg/kg) labeling in young C57Bl mice. Immunoreactivity to anti-recoverin, rhodopsin, and Pax6 antibodies and binding to peanut agglutinin were analyzed histologically to assess the distribution and differentiation of photoreceptor progenitors or precursors. Mice injected intraperitoneally with N-methyl-N-nitrosourea (MNU; 60 mg/kg) were also examined. Part of the neuroblast layer composed of BrdU-positive retinal progenitor cells was identified within the ciliary epithelium of the pars plana in continuation of the layer of the peripheral retina during ocular development. In both the ciliary epithelium and the retina, the layer size decreased rapidly and disappeared mostly by postnatal day (P)9. Within the ciliary epithelium of the pars plana, numerous postmitotic rod and cone photoreceptor precursors were identified that rapidly differentiated morphologically and decreased in number with ocular development. Rod precursors were no longer seen in the pars plana at P12, whereas rare presumptive cone precursors persisted even at P120. An increase in the number of presumptive cone precursors (approximately 16-fold) was identified in the pars plana of adult mice with MNU-induced photoreceptor degeneration. Rod and cone precursors were identified in the ciliary epithelium of the murine pars plana during ocular development but nearly disappeared after the completion of histogenesis. However, in response to retinal injury, an increased number of presumptive cone precursors was found even in the adult pars plana.</abstract><cop>Rockville, MD</cop><pub>ARVO</pub><pmid>18172121</pmid><doi>10.1167/iovs.07-1008</doi><tpages>7</tpages></addata></record>
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source MEDLINE; Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals; PubMed Central
subjects Animals
Biological and medical sciences
Bromodeoxyuridine - metabolism
Cell Differentiation
Ciliary Body - cytology
Eye - growth & development
Eye and associated structures. Visual pathways and centers. Vision
Eye Proteins - metabolism
Fluorescent Antibody Technique, Indirect
Fundamental and applied biological sciences. Psychology
Homeodomain Proteins - metabolism
Medical sciences
Methylnitrosourea - toxicity
Mice
Mice, Inbred C57BL
Microscopy, Confocal
Ophthalmology
Paired Box Transcription Factors - metabolism
PAX6 Transcription Factor
Photoreceptor Cells, Vertebrate - cytology
Photoreceptor Cells, Vertebrate - metabolism
Pigment Epithelium of Eye - cytology
Recoverin - metabolism
Repressor Proteins - metabolism
Retinal Degeneration - chemically induced
Retinal Degeneration - pathology
Rhodopsin - metabolism
Stem Cells - cytology
Stem Cells - metabolism
Vertebrates: nervous system and sense organs
title Identification of Photoreceptor Precursors in the Pars Plana during Ocular Development and after Retinal Injury
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