Identification of a Novel Secreted Protease from Pseudomonas aeruginosa that Causes Corneal Erosions

The purpose of this study was to identify a new Pseudomonas protease and determine its possible role in keratitis. Concentrated culture supernatants of the Pseudomonas aeruginosa strains PA103 and ATCC 19660 were analyzed by zymography. P. aeruginosa small protease (PASP) was purified from strain PA...

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Veröffentlicht in:	Investigative ophthalmology & visual science 2005-10, Vol.46 (10), p.3761-3768
Hauptverfasser:	Marquart, Mary E, Caballero, Armando R, Chomnawang, Mullika, Thibodeaux, Brett A, Twining, Sally S, O'Callaghan, Richard J
Format:	Artikel
Sprache:	eng
Schlagworte:	Amino Acid Sequence Animals Bacterial Proteins - isolation & purification Bacterial Proteins - pharmacology Base Sequence Biological and medical sciences Blotting, Western Cloning, Molecular Cornea - drug effects Cornea - pathology Corneal Diseases - chemically induced Corneal Diseases - pathology Diseases of cornea, anterior segment and sclera Electrophoresis, Polyacrylamide Gel Escherichia coli - genetics Eye and associated structures. Visual pathways and centers. Vision Fundamental and applied biological sciences. Psychology Gene Expression Medical sciences Metalloendopeptidases - isolation & purification Metalloendopeptidases - pharmacology Molecular Sequence Data Molecular Weight Ophthalmology Pseudomonas aeruginosa - enzymology Rabbits Recombinant Proteins Serine Endopeptidases - genetics Serine Endopeptidases - isolation & purification Serine Endopeptidases - pharmacology Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization Vertebrates: nervous system and sense organs
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