Clones of Interstitial Cells From Bovine Aortic Valve Exhibit Different Calcifying Potential When Exposed to Endotoxin and Phosphate
OBJECTIVE—Our purpose was to study in vitro whether phenotypically-distinct interstitial cell clones from bovine aortic valve (BVIC) possess different calcifying potential in response to endotoxin (lipopolysaccharide [LPS]) and phosphate (Pi). METHODS AND RESULTS—Among various clones of BVIC obtaine...
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| Veröffentlicht in: | Arteriosclerosis, thrombosis, and vascular biology thrombosis, and vascular biology, 2008-12, Vol.28 (12), p.2165-2172 |
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| creator | Rattazzi, Marcello Iop, Laura Faggin, Elisabetta Bertacco, Elisa Zoppellaro, Giacomo Baesso, Ilenia Puato, Massimo Torregrossa, Gianluca Fadini, Gian Paolo Agostini, Carlo Gerosa, Gino Sartore, Saverio Pauletto, Paolo |
| description | OBJECTIVE—Our purpose was to study in vitro whether phenotypically-distinct interstitial cell clones from bovine aortic valve (BVIC) possess different calcifying potential in response to endotoxin (lipopolysaccharide [LPS]) and phosphate (Pi).
METHODS AND RESULTS—Among various clones of BVIC obtained by limited dilution technique we selected 4 clones displaying different growth patterns and immunophenotypes. Uncloned and cloned cells were treated with combinations of LPS (100 ng/mL) and Pi (2.4 mmol/L). Uncloned BVIC showed increased alkaline phosphatase activity (ALP) after treatment with LPS, which resulted in calcification after addition of Pi. Among BVIC clones, only Clone 1 (fibroblast-like phenotype) showed a relevant increase in ALP after LPS treatment in parallel with prevention of smooth muscle (SM) α-actin accumulation. No effect was observed in clonal cells harboring a more stable SM cell-like profile (Clone 4). None of the isolated clones calcified but mineralization was induced in the presence of LPS plus Pi when Clone 1 was cocultured with Clone 4 or after seeding on type I collagen sponges.
CONCLUSION—Endotoxin and phosphate can act as valve calcification promoters by targeting specific fibroblast-like interstitial valve cells that possess a unique procalcific potential. |
| doi_str_mv | 10.1161/ATVBAHA.108.174342 |
| format | Article |
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METHODS AND RESULTS—Among various clones of BVIC obtained by limited dilution technique we selected 4 clones displaying different growth patterns and immunophenotypes. Uncloned and cloned cells were treated with combinations of LPS (100 ng/mL) and Pi (2.4 mmol/L). Uncloned BVIC showed increased alkaline phosphatase activity (ALP) after treatment with LPS, which resulted in calcification after addition of Pi. Among BVIC clones, only Clone 1 (fibroblast-like phenotype) showed a relevant increase in ALP after LPS treatment in parallel with prevention of smooth muscle (SM) α-actin accumulation. No effect was observed in clonal cells harboring a more stable SM cell-like profile (Clone 4). None of the isolated clones calcified but mineralization was induced in the presence of LPS plus Pi when Clone 1 was cocultured with Clone 4 or after seeding on type I collagen sponges.
CONCLUSION—Endotoxin and phosphate can act as valve calcification promoters by targeting specific fibroblast-like interstitial valve cells that possess a unique procalcific potential.</description><identifier>ISSN: 1079-5642</identifier><identifier>EISSN: 1524-4636</identifier><identifier>DOI: 10.1161/ATVBAHA.108.174342</identifier><identifier>PMID: 18832754</identifier><identifier>CODEN: ATVBFA</identifier><language>eng</language><publisher>Philadelphia, PA: American Heart Association, Inc</publisher><subject>Alkaline Phosphatase - metabolism ; Animals ; Aortic Valve - drug effects ; Aortic Valve - metabolism ; Aortic Valve - pathology ; Atherosclerosis (general aspects, experimental research) ; Biological and medical sciences ; Blood and lymphatic vessels ; Calcinosis - etiology ; Calcinosis - metabolism ; Calcinosis - pathology ; Calcium - metabolism ; Cardiology. Vascular system ; Cattle ; Clone Cells ; Coculture Techniques ; Diseases of the aorta ; Diseases of the peripheral vessels. Diseases of the vena cava. Miscellaneous ; Fibroblasts - drug effects ; Fibroblasts - metabolism ; Fibroblasts - pathology ; Heart Valve Diseases - etiology ; Heart Valve Diseases - metabolism ; Heart Valve Diseases - pathology ; Lipopolysaccharides - toxicity ; Medical sciences ; Myocytes, Smooth Muscle - drug effects ; Myocytes, Smooth Muscle - metabolism ; Myocytes, Smooth Muscle - pathology ; Phenotype ; Phosphates - toxicity</subject><ispartof>Arteriosclerosis, thrombosis, and vascular biology, 2008-12, Vol.28 (12), p.2165-2172</ispartof><rights>2008 American Heart Association, Inc.</rights><rights>2009 INIST-CNRS</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c5214-72b1ddc64837ad5e2003cb7165ba04bdf2d0fc58faa797b57b69adbcea10f9e43</citedby><cites>FETCH-LOGICAL-c5214-72b1ddc64837ad5e2003cb7165ba04bdf2d0fc58faa797b57b69adbcea10f9e43</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,776,780,27901,27902</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=20899456$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/18832754$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Rattazzi, Marcello</creatorcontrib><creatorcontrib>Iop, Laura</creatorcontrib><creatorcontrib>Faggin, Elisabetta</creatorcontrib><creatorcontrib>Bertacco, Elisa</creatorcontrib><creatorcontrib>Zoppellaro, Giacomo</creatorcontrib><creatorcontrib>Baesso, Ilenia</creatorcontrib><creatorcontrib>Puato, Massimo</creatorcontrib><creatorcontrib>Torregrossa, Gianluca</creatorcontrib><creatorcontrib>Fadini, Gian Paolo</creatorcontrib><creatorcontrib>Agostini, Carlo</creatorcontrib><creatorcontrib>Gerosa, Gino</creatorcontrib><creatorcontrib>Sartore, Saverio</creatorcontrib><creatorcontrib>Pauletto, Paolo</creatorcontrib><title>Clones of Interstitial Cells From Bovine Aortic Valve Exhibit Different Calcifying Potential When Exposed to Endotoxin and Phosphate</title><title>Arteriosclerosis, thrombosis, and vascular biology</title><addtitle>Arterioscler Thromb Vasc Biol</addtitle><description>OBJECTIVE—Our purpose was to study in vitro whether phenotypically-distinct interstitial cell clones from bovine aortic valve (BVIC) possess different calcifying potential in response to endotoxin (lipopolysaccharide [LPS]) and phosphate (Pi).
METHODS AND RESULTS—Among various clones of BVIC obtained by limited dilution technique we selected 4 clones displaying different growth patterns and immunophenotypes. Uncloned and cloned cells were treated with combinations of LPS (100 ng/mL) and Pi (2.4 mmol/L). Uncloned BVIC showed increased alkaline phosphatase activity (ALP) after treatment with LPS, which resulted in calcification after addition of Pi. Among BVIC clones, only Clone 1 (fibroblast-like phenotype) showed a relevant increase in ALP after LPS treatment in parallel with prevention of smooth muscle (SM) α-actin accumulation. No effect was observed in clonal cells harboring a more stable SM cell-like profile (Clone 4). None of the isolated clones calcified but mineralization was induced in the presence of LPS plus Pi when Clone 1 was cocultured with Clone 4 or after seeding on type I collagen sponges.
CONCLUSION—Endotoxin and phosphate can act as valve calcification promoters by targeting specific fibroblast-like interstitial valve cells that possess a unique procalcific potential.</description><subject>Alkaline Phosphatase - metabolism</subject><subject>Animals</subject><subject>Aortic Valve - drug effects</subject><subject>Aortic Valve - metabolism</subject><subject>Aortic Valve - pathology</subject><subject>Atherosclerosis (general aspects, experimental research)</subject><subject>Biological and medical sciences</subject><subject>Blood and lymphatic vessels</subject><subject>Calcinosis - etiology</subject><subject>Calcinosis - metabolism</subject><subject>Calcinosis - pathology</subject><subject>Calcium - metabolism</subject><subject>Cardiology. Vascular system</subject><subject>Cattle</subject><subject>Clone Cells</subject><subject>Coculture Techniques</subject><subject>Diseases of the aorta</subject><subject>Diseases of the peripheral vessels. Diseases of the vena cava. Miscellaneous</subject><subject>Fibroblasts - drug effects</subject><subject>Fibroblasts - metabolism</subject><subject>Fibroblasts - pathology</subject><subject>Heart Valve Diseases - etiology</subject><subject>Heart Valve Diseases - metabolism</subject><subject>Heart Valve Diseases - pathology</subject><subject>Lipopolysaccharides - toxicity</subject><subject>Medical sciences</subject><subject>Myocytes, Smooth Muscle - drug effects</subject><subject>Myocytes, Smooth Muscle - metabolism</subject><subject>Myocytes, Smooth Muscle - pathology</subject><subject>Phenotype</subject><subject>Phosphates - toxicity</subject><issn>1079-5642</issn><issn>1524-4636</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2008</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNpFkUtv3CAURq2qVZMm_QNdVGy69BQwD3vpOJOHFKlZpMnS4lnTMjAC8tr3h4fRjNoFFy4635U4NM0XBFcIMfR9vLs_G6_GFYL9CnHSEfyuOUYUk5awjr2vZ8iHljKCj5pPOf-GEBKM4cfmCPV9hzklx83fycdgMogWXIdiUi6uOOHBZLzP4CLFDTiLTy4YMMZUnAL3wj8ZsH5ZnHQFnDtrTTKhgEl45eyrC7_AbSz1ZjflYTGhstuYjQYlgnXQscQXF4AIGtwuMW8XUcxp88EKn83nw37S_LxY301X7c2Py-tpvGkVxYi0HEuktWKk77jQ1GAIOyU5YlQKSKS2WEOraG-F4AOXlEs2CC2VEQjawZDupMH7uSrFnJOx8za5jUivM4LzTul8UFr7ft4rraGv-9D2UW6M_h85OKzAtwMgshLeJhGUy_84DPthIJRVjuy55-h3pv_4x2eT5sUIX5Z59zkdg7Str-pRLbCtC5HuDZhykTc</recordid><startdate>200812</startdate><enddate>200812</enddate><creator>Rattazzi, Marcello</creator><creator>Iop, Laura</creator><creator>Faggin, Elisabetta</creator><creator>Bertacco, Elisa</creator><creator>Zoppellaro, Giacomo</creator><creator>Baesso, Ilenia</creator><creator>Puato, Massimo</creator><creator>Torregrossa, Gianluca</creator><creator>Fadini, Gian Paolo</creator><creator>Agostini, Carlo</creator><creator>Gerosa, Gino</creator><creator>Sartore, Saverio</creator><creator>Pauletto, Paolo</creator><general>American Heart Association, Inc</general><general>Lippincott Williams & Wilkins</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope></search><sort><creationdate>200812</creationdate><title>Clones of Interstitial Cells From Bovine Aortic Valve Exhibit Different Calcifying Potential When Exposed to Endotoxin and Phosphate</title><author>Rattazzi, Marcello ; Iop, Laura ; Faggin, Elisabetta ; Bertacco, Elisa ; Zoppellaro, Giacomo ; Baesso, Ilenia ; Puato, Massimo ; Torregrossa, Gianluca ; Fadini, Gian Paolo ; Agostini, Carlo ; Gerosa, Gino ; Sartore, Saverio ; Pauletto, Paolo</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c5214-72b1ddc64837ad5e2003cb7165ba04bdf2d0fc58faa797b57b69adbcea10f9e43</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2008</creationdate><topic>Alkaline Phosphatase - metabolism</topic><topic>Animals</topic><topic>Aortic Valve - drug effects</topic><topic>Aortic Valve - metabolism</topic><topic>Aortic Valve - pathology</topic><topic>Atherosclerosis (general aspects, experimental research)</topic><topic>Biological and medical sciences</topic><topic>Blood and lymphatic vessels</topic><topic>Calcinosis - etiology</topic><topic>Calcinosis - metabolism</topic><topic>Calcinosis - pathology</topic><topic>Calcium - metabolism</topic><topic>Cardiology. Vascular system</topic><topic>Cattle</topic><topic>Clone Cells</topic><topic>Coculture Techniques</topic><topic>Diseases of the aorta</topic><topic>Diseases of the peripheral vessels. Diseases of the vena cava. Miscellaneous</topic><topic>Fibroblasts - drug effects</topic><topic>Fibroblasts - metabolism</topic><topic>Fibroblasts - pathology</topic><topic>Heart Valve Diseases - etiology</topic><topic>Heart Valve Diseases - metabolism</topic><topic>Heart Valve Diseases - pathology</topic><topic>Lipopolysaccharides - toxicity</topic><topic>Medical sciences</topic><topic>Myocytes, Smooth Muscle - drug effects</topic><topic>Myocytes, Smooth Muscle - metabolism</topic><topic>Myocytes, Smooth Muscle - pathology</topic><topic>Phenotype</topic><topic>Phosphates - toxicity</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Rattazzi, Marcello</creatorcontrib><creatorcontrib>Iop, Laura</creatorcontrib><creatorcontrib>Faggin, Elisabetta</creatorcontrib><creatorcontrib>Bertacco, Elisa</creatorcontrib><creatorcontrib>Zoppellaro, Giacomo</creatorcontrib><creatorcontrib>Baesso, Ilenia</creatorcontrib><creatorcontrib>Puato, Massimo</creatorcontrib><creatorcontrib>Torregrossa, Gianluca</creatorcontrib><creatorcontrib>Fadini, Gian Paolo</creatorcontrib><creatorcontrib>Agostini, Carlo</creatorcontrib><creatorcontrib>Gerosa, Gino</creatorcontrib><creatorcontrib>Sartore, Saverio</creatorcontrib><creatorcontrib>Pauletto, Paolo</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><jtitle>Arteriosclerosis, thrombosis, and vascular biology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Rattazzi, Marcello</au><au>Iop, Laura</au><au>Faggin, Elisabetta</au><au>Bertacco, Elisa</au><au>Zoppellaro, Giacomo</au><au>Baesso, Ilenia</au><au>Puato, Massimo</au><au>Torregrossa, Gianluca</au><au>Fadini, Gian Paolo</au><au>Agostini, Carlo</au><au>Gerosa, Gino</au><au>Sartore, Saverio</au><au>Pauletto, Paolo</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Clones of Interstitial Cells From Bovine Aortic Valve Exhibit Different Calcifying Potential When Exposed to Endotoxin and Phosphate</atitle><jtitle>Arteriosclerosis, thrombosis, and vascular biology</jtitle><addtitle>Arterioscler Thromb Vasc Biol</addtitle><date>2008-12</date><risdate>2008</risdate><volume>28</volume><issue>12</issue><spage>2165</spage><epage>2172</epage><pages>2165-2172</pages><issn>1079-5642</issn><eissn>1524-4636</eissn><coden>ATVBFA</coden><abstract>OBJECTIVE—Our purpose was to study in vitro whether phenotypically-distinct interstitial cell clones from bovine aortic valve (BVIC) possess different calcifying potential in response to endotoxin (lipopolysaccharide [LPS]) and phosphate (Pi).
METHODS AND RESULTS—Among various clones of BVIC obtained by limited dilution technique we selected 4 clones displaying different growth patterns and immunophenotypes. Uncloned and cloned cells were treated with combinations of LPS (100 ng/mL) and Pi (2.4 mmol/L). Uncloned BVIC showed increased alkaline phosphatase activity (ALP) after treatment with LPS, which resulted in calcification after addition of Pi. Among BVIC clones, only Clone 1 (fibroblast-like phenotype) showed a relevant increase in ALP after LPS treatment in parallel with prevention of smooth muscle (SM) α-actin accumulation. No effect was observed in clonal cells harboring a more stable SM cell-like profile (Clone 4). None of the isolated clones calcified but mineralization was induced in the presence of LPS plus Pi when Clone 1 was cocultured with Clone 4 or after seeding on type I collagen sponges.
CONCLUSION—Endotoxin and phosphate can act as valve calcification promoters by targeting specific fibroblast-like interstitial valve cells that possess a unique procalcific potential.</abstract><cop>Philadelphia, PA</cop><pub>American Heart Association, Inc</pub><pmid>18832754</pmid><doi>10.1161/ATVBAHA.108.174342</doi><tpages>8</tpages><oa>free_for_read</oa></addata></record> |
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| identifier | ISSN: 1079-5642 |
| ispartof | Arteriosclerosis, thrombosis, and vascular biology, 2008-12, Vol.28 (12), p.2165-2172 |
| issn | 1079-5642 1524-4636 |
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| source | MEDLINE; Alma/SFX Local Collection; Journals@Ovid Complete |
| subjects | Alkaline Phosphatase - metabolism Animals Aortic Valve - drug effects Aortic Valve - metabolism Aortic Valve - pathology Atherosclerosis (general aspects, experimental research) Biological and medical sciences Blood and lymphatic vessels Calcinosis - etiology Calcinosis - metabolism Calcinosis - pathology Calcium - metabolism Cardiology. Vascular system Cattle Clone Cells Coculture Techniques Diseases of the aorta Diseases of the peripheral vessels. Diseases of the vena cava. Miscellaneous Fibroblasts - drug effects Fibroblasts - metabolism Fibroblasts - pathology Heart Valve Diseases - etiology Heart Valve Diseases - metabolism Heart Valve Diseases - pathology Lipopolysaccharides - toxicity Medical sciences Myocytes, Smooth Muscle - drug effects Myocytes, Smooth Muscle - metabolism Myocytes, Smooth Muscle - pathology Phenotype Phosphates - toxicity |
| title | Clones of Interstitial Cells From Bovine Aortic Valve Exhibit Different Calcifying Potential When Exposed to Endotoxin and Phosphate |
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