miR-142-5p Improves Neural Differentiation and Proliferation of Adipose-Derived Stem Cells
Background/Aims: MiRNAs may regulate neurogenic differentiation of adipose-derived stem cells (ADSCs). In this study, we hypothesized that the miR-142-5p can repress the expression of RhoA/ROCK1 pathway on the neurogenesis of ADSCs. Methods: Deregulated miRNA during neurogenic differentiation of ADS...
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| Veröffentlicht in: | Cellular physiology and biochemistry 2018-01, Vol.50 (6), p.2097-2107 |
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| description | Background/Aims: MiRNAs may regulate neurogenic differentiation of adipose-derived stem cells (ADSCs). In this study, we hypothesized that the miR-142-5p can repress the expression of RhoA/ROCK1 pathway on the neurogenesis of ADSCs. Methods: Deregulated miRNA during neurogenic differentiation of ADSCs were identified. The expression of neuron-specific enolase (NSE) and β III tubulin (Neuron-specific class III beta-tubulin) were detected as the markers of neurogenic differentiation by immunostaining and western blot. The targeting of miR-142-5p on RhoA and ROCK1 was verified by dual luciferase assay, qRT-PCR and western blot. The roles of miR-142-5p and the RhoA/ROCK1 signaling pathway were explored by using functional experiments including cell viability and colony formation assays. Results: MiR-142-5p is significantly upregulated during neurogenic differentiation of ADSCs. Knockdown of endogenous miR-142-5p hampered neurogenic differentiation. MiR-142-5p could directly target RhoA and ROCK1 mRNA and repress their expressions, through which it increased the proportion of differentiated cells with positive NSE and β III tubulin. RhoA/ROCK1 signaling pathway is involved in miR-142-5p effect on the process of neurogenic differentiation of ADSCs. Conclusion: Our results demonstrate that miR-142-5p functions as a growth promotive miRNA and plays an important role in neurogenic differentiation by targeting RhoA/ROCK1 in ADSCs. |
| doi_str_mv | 10.1159/000495054 |
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In this study, we hypothesized that the miR-142-5p can repress the expression of RhoA/ROCK1 pathway on the neurogenesis of ADSCs. Methods: Deregulated miRNA during neurogenic differentiation of ADSCs were identified. The expression of neuron-specific enolase (NSE) and β III tubulin (Neuron-specific class III beta-tubulin) were detected as the markers of neurogenic differentiation by immunostaining and western blot. The targeting of miR-142-5p on RhoA and ROCK1 was verified by dual luciferase assay, qRT-PCR and western blot. The roles of miR-142-5p and the RhoA/ROCK1 signaling pathway were explored by using functional experiments including cell viability and colony formation assays. Results: MiR-142-5p is significantly upregulated during neurogenic differentiation of ADSCs. Knockdown of endogenous miR-142-5p hampered neurogenic differentiation. MiR-142-5p could directly target RhoA and ROCK1 mRNA and repress their expressions, through which it increased the proportion of differentiated cells with positive NSE and β III tubulin. RhoA/ROCK1 signaling pathway is involved in miR-142-5p effect on the process of neurogenic differentiation of ADSCs. Conclusion: Our results demonstrate that miR-142-5p functions as a growth promotive miRNA and plays an important role in neurogenic differentiation by targeting RhoA/ROCK1 in ADSCs.</description><identifier>ISSN: 1015-8987</identifier><identifier>EISSN: 1421-9778</identifier><identifier>DOI: 10.1159/000495054</identifier><identifier>PMID: 30415244</identifier><language>eng</language><publisher>Basel, Switzerland: S. Karger AG</publisher><subject>Adipose Tissue - cytology ; Adipose-derived stem cells ; Alzheimer's disease ; Antagomirs - metabolism ; Cell culture ; Cell cycle ; Cell Differentiation ; Cell Line ; Cell Proliferation ; Gene expression ; Growth factors ; Humans ; Kinases ; MicroRNAs ; MicroRNAs - antagonists & inhibitors ; MicroRNAs - genetics ; MicroRNAs - metabolism ; miR-142-5p ; Neurogenic differentiation ; Neurons - cytology ; Neurons - metabolism ; Original Paper ; Plasmids ; rho-Associated Kinases - antagonists & inhibitors ; rho-Associated Kinases - genetics ; rho-Associated Kinases - metabolism ; RhoA ; rhoA GTP-Binding Protein - antagonists & inhibitors ; rhoA GTP-Binding Protein - genetics ; rhoA GTP-Binding Protein - metabolism ; RNA Interference ; RNA, Small Interfering - metabolism ; ROCK1 ; Signal Transduction ; Stem cells ; Stem Cells - cytology ; Stem Cells - metabolism ; Tubulin - metabolism</subject><ispartof>Cellular physiology and biochemistry, 2018-01, Vol.50 (6), p.2097-2107</ispartof><rights>2018 The Author(s). Published by S. Karger AG, Basel</rights><rights>2018 The Author(s). Published by S. Karger AG, Basel.</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c463t-5c769146409a37a59599d5500333de5fa5a107fc4bdda1772aa05b6eec0934ab3</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,864,2102,27635,27924,27925</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/30415244$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Yang, Liang</creatorcontrib><creatorcontrib>Wang, Zhi-Fei</creatorcontrib><creatorcontrib>Wu, Hao</creatorcontrib><creatorcontrib>Wang, Wei</creatorcontrib><title>miR-142-5p Improves Neural Differentiation and Proliferation of Adipose-Derived Stem Cells</title><title>Cellular physiology and biochemistry</title><addtitle>Cell Physiol Biochem</addtitle><description>Background/Aims: MiRNAs may regulate neurogenic differentiation of adipose-derived stem cells (ADSCs). In this study, we hypothesized that the miR-142-5p can repress the expression of RhoA/ROCK1 pathway on the neurogenesis of ADSCs. Methods: Deregulated miRNA during neurogenic differentiation of ADSCs were identified. The expression of neuron-specific enolase (NSE) and β III tubulin (Neuron-specific class III beta-tubulin) were detected as the markers of neurogenic differentiation by immunostaining and western blot. The targeting of miR-142-5p on RhoA and ROCK1 was verified by dual luciferase assay, qRT-PCR and western blot. The roles of miR-142-5p and the RhoA/ROCK1 signaling pathway were explored by using functional experiments including cell viability and colony formation assays. Results: MiR-142-5p is significantly upregulated during neurogenic differentiation of ADSCs. Knockdown of endogenous miR-142-5p hampered neurogenic differentiation. MiR-142-5p could directly target RhoA and ROCK1 mRNA and repress their expressions, through which it increased the proportion of differentiated cells with positive NSE and β III tubulin. RhoA/ROCK1 signaling pathway is involved in miR-142-5p effect on the process of neurogenic differentiation of ADSCs. Conclusion: Our results demonstrate that miR-142-5p functions as a growth promotive miRNA and plays an important role in neurogenic differentiation by targeting RhoA/ROCK1 in ADSCs.</description><subject>Adipose Tissue - cytology</subject><subject>Adipose-derived stem cells</subject><subject>Alzheimer's disease</subject><subject>Antagomirs - metabolism</subject><subject>Cell culture</subject><subject>Cell cycle</subject><subject>Cell Differentiation</subject><subject>Cell Line</subject><subject>Cell Proliferation</subject><subject>Gene expression</subject><subject>Growth factors</subject><subject>Humans</subject><subject>Kinases</subject><subject>MicroRNAs</subject><subject>MicroRNAs - antagonists & inhibitors</subject><subject>MicroRNAs - genetics</subject><subject>MicroRNAs - metabolism</subject><subject>miR-142-5p</subject><subject>Neurogenic differentiation</subject><subject>Neurons - cytology</subject><subject>Neurons - metabolism</subject><subject>Original Paper</subject><subject>Plasmids</subject><subject>rho-Associated Kinases - antagonists & inhibitors</subject><subject>rho-Associated Kinases - genetics</subject><subject>rho-Associated Kinases - metabolism</subject><subject>RhoA</subject><subject>rhoA GTP-Binding Protein - antagonists & inhibitors</subject><subject>rhoA GTP-Binding Protein - genetics</subject><subject>rhoA GTP-Binding Protein - metabolism</subject><subject>RNA Interference</subject><subject>RNA, Small Interfering - metabolism</subject><subject>ROCK1</subject><subject>Signal Transduction</subject><subject>Stem cells</subject><subject>Stem Cells - cytology</subject><subject>Stem Cells - metabolism</subject><subject>Tubulin - metabolism</subject><issn>1015-8987</issn><issn>1421-9778</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2018</creationdate><recordtype>article</recordtype><sourceid>M--</sourceid><sourceid>EIF</sourceid><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DOA</sourceid><recordid>eNptkUtv1TAQhSMEoqWwYI9QpG7oIuBnHC_LLYUrVVDx2LCxJvak8iWJUzupxL_HkJIFYmX7-JszHp-ieE7Ja0qlfkMIEVoSKR4Ux1QwWmmlmod5T6isGt2oo-JJSgeSj0qzx8URJ4JKJsRx8X3wn6tcU8mp3A9TDHeYyo-4ROjLC991GHGcPcw-jCWMrryOofdZXZXQlefOTyFhdYHR36Erv8w4lDvs-_S0eNRBn_DZ_XpSfLt893X3obr69H6_O7-qrKj5XEmrak1FLYgGrkBqqbWTkhDOuUPZgQRKVGdF6xxQpRgAkW2NaInmAlp-UuxXXxfgYKboB4g_TQBv_ggh3hiIs7c9Gm45oV0tWmAohLKNoA1hDYOasJbzJnu9Wr3yT9wumGYz-GTzNDBiWJJhlDMmm9w4o6f_oIewxDFPalju0mitSZ2ps5WyMaQUsdseSIn5HZ7Zwsvsy3vHpR3QbeTftDLwYgV-QLzBuAFb_el_r3fXb1fCTK7jvwAQN6Ue</recordid><startdate>20180101</startdate><enddate>20180101</enddate><creator>Yang, Liang</creator><creator>Wang, Zhi-Fei</creator><creator>Wu, Hao</creator><creator>Wang, Wei</creator><general>S. Karger AG</general><general>Cell Physiol Biochem Press GmbH & Co KG</general><scope>M--</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7X7</scope><scope>7XB</scope><scope>88E</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>BENPR</scope><scope>CCPQU</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>K9.</scope><scope>M0S</scope><scope>M1P</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>7X8</scope><scope>DOA</scope></search><sort><creationdate>20180101</creationdate><title>miR-142-5p Improves Neural Differentiation and Proliferation of Adipose-Derived Stem Cells</title><author>Yang, Liang ; Wang, Zhi-Fei ; Wu, Hao ; Wang, Wei</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c463t-5c769146409a37a59599d5500333de5fa5a107fc4bdda1772aa05b6eec0934ab3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2018</creationdate><topic>Adipose Tissue - cytology</topic><topic>Adipose-derived stem cells</topic><topic>Alzheimer's disease</topic><topic>Antagomirs - metabolism</topic><topic>Cell culture</topic><topic>Cell cycle</topic><topic>Cell Differentiation</topic><topic>Cell Line</topic><topic>Cell Proliferation</topic><topic>Gene expression</topic><topic>Growth factors</topic><topic>Humans</topic><topic>Kinases</topic><topic>MicroRNAs</topic><topic>MicroRNAs - antagonists & inhibitors</topic><topic>MicroRNAs - genetics</topic><topic>MicroRNAs - metabolism</topic><topic>miR-142-5p</topic><topic>Neurogenic differentiation</topic><topic>Neurons - cytology</topic><topic>Neurons - metabolism</topic><topic>Original Paper</topic><topic>Plasmids</topic><topic>rho-Associated Kinases - antagonists & inhibitors</topic><topic>rho-Associated Kinases - genetics</topic><topic>rho-Associated Kinases - metabolism</topic><topic>RhoA</topic><topic>rhoA GTP-Binding Protein - antagonists & inhibitors</topic><topic>rhoA GTP-Binding Protein - genetics</topic><topic>rhoA GTP-Binding Protein - metabolism</topic><topic>RNA Interference</topic><topic>RNA, Small Interfering - metabolism</topic><topic>ROCK1</topic><topic>Signal Transduction</topic><topic>Stem cells</topic><topic>Stem Cells - cytology</topic><topic>Stem Cells - metabolism</topic><topic>Tubulin - metabolism</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Yang, Liang</creatorcontrib><creatorcontrib>Wang, Zhi-Fei</creatorcontrib><creatorcontrib>Wu, Hao</creatorcontrib><creatorcontrib>Wang, Wei</creatorcontrib><collection>Karger Open Access</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Medical Database (Alumni Edition)</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest Central UK/Ireland</collection><collection>ProQuest Central</collection><collection>ProQuest One Community College</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Medical Database</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central China</collection><collection>MEDLINE - Academic</collection><collection>DOAJ Directory of Open Access Journals</collection><jtitle>Cellular physiology and biochemistry</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Yang, Liang</au><au>Wang, Zhi-Fei</au><au>Wu, Hao</au><au>Wang, Wei</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>miR-142-5p Improves Neural Differentiation and Proliferation of Adipose-Derived Stem Cells</atitle><jtitle>Cellular physiology and biochemistry</jtitle><addtitle>Cell Physiol Biochem</addtitle><date>2018-01-01</date><risdate>2018</risdate><volume>50</volume><issue>6</issue><spage>2097</spage><epage>2107</epage><pages>2097-2107</pages><issn>1015-8987</issn><eissn>1421-9778</eissn><abstract>Background/Aims: MiRNAs may regulate neurogenic differentiation of adipose-derived stem cells (ADSCs). In this study, we hypothesized that the miR-142-5p can repress the expression of RhoA/ROCK1 pathway on the neurogenesis of ADSCs. Methods: Deregulated miRNA during neurogenic differentiation of ADSCs were identified. The expression of neuron-specific enolase (NSE) and β III tubulin (Neuron-specific class III beta-tubulin) were detected as the markers of neurogenic differentiation by immunostaining and western blot. The targeting of miR-142-5p on RhoA and ROCK1 was verified by dual luciferase assay, qRT-PCR and western blot. The roles of miR-142-5p and the RhoA/ROCK1 signaling pathway were explored by using functional experiments including cell viability and colony formation assays. Results: MiR-142-5p is significantly upregulated during neurogenic differentiation of ADSCs. Knockdown of endogenous miR-142-5p hampered neurogenic differentiation. MiR-142-5p could directly target RhoA and ROCK1 mRNA and repress their expressions, through which it increased the proportion of differentiated cells with positive NSE and β III tubulin. RhoA/ROCK1 signaling pathway is involved in miR-142-5p effect on the process of neurogenic differentiation of ADSCs. Conclusion: Our results demonstrate that miR-142-5p functions as a growth promotive miRNA and plays an important role in neurogenic differentiation by targeting RhoA/ROCK1 in ADSCs.</abstract><cop>Basel, Switzerland</cop><pub>S. Karger AG</pub><pmid>30415244</pmid><doi>10.1159/000495054</doi><tpages>11</tpages><oa>free_for_read</oa></addata></record> |
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| subjects | Adipose Tissue - cytology Adipose-derived stem cells Alzheimer's disease Antagomirs - metabolism Cell culture Cell cycle Cell Differentiation Cell Line Cell Proliferation Gene expression Growth factors Humans Kinases MicroRNAs MicroRNAs - antagonists & inhibitors MicroRNAs - genetics MicroRNAs - metabolism miR-142-5p Neurogenic differentiation Neurons - cytology Neurons - metabolism Original Paper Plasmids rho-Associated Kinases - antagonists & inhibitors rho-Associated Kinases - genetics rho-Associated Kinases - metabolism RhoA rhoA GTP-Binding Protein - antagonists & inhibitors rhoA GTP-Binding Protein - genetics rhoA GTP-Binding Protein - metabolism RNA Interference RNA, Small Interfering - metabolism ROCK1 Signal Transduction Stem cells Stem Cells - cytology Stem Cells - metabolism Tubulin - metabolism |
| title | miR-142-5p Improves Neural Differentiation and Proliferation of Adipose-Derived Stem Cells |
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