Identifying Signalling Pathways Regulated by GPRC5B in β-Cells by CRISPR-Cas9-Mediated Genome Editing

Background/Aims: CRISPR-Cas9, a RNA-guided targeted genome editing tool, has revolutionized genetic engineering by offering the ability to precisely modify DNA. GPRC5B is an orphan receptor belonging to the group C family of G protein-coupled receptors (GPCRs). In this study, we analysed the functio...

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Veröffentlicht in:	Cellular physiology and biochemistry 2018-01, Vol.45 (2), p.656-666
Hauptverfasser:	Atanes, Patricio, Ruz-Maldonado, Inmaculada, Hawkes, Ross, Liu, Bo, Persaud, Shanta J., Amisten, Stefan
Format:	Artikel
Sprache:	eng
Schlagworte:	Animals Apoptosis Apoptosis - drug effects Apoptosis Regulatory Proteins - metabolism Base Sequence Cell culture Cell growth Cell Line, Tumor Cell Proliferation Cloning CRISPR CRISPR-Cas Systems - genetics CRISPR-Cas9 Deoxyribonucleic acid Diabetes DNA Gene Editing Genetic engineering Genome editing Genomes Genomics Glucose GPRC5B Insulin-Secreting Cells - cytology Insulin-Secreting Cells - metabolism Interferon-gamma - metabolism Mice Neuropeptides - metabolism Original Paper Palmitic Acid - toxicity Phosphorylation Plasmids Plasmids - genetics Plasmids - metabolism Proliferation Proteins Receptors, G-Protein-Coupled - antagonists & inhibitors Receptors, G-Protein-Coupled - genetics Receptors, G-Protein-Coupled - metabolism Signal Transduction Smad2 Protein - metabolism Smad3 Protein - metabolism STAT1 Transcription Factor - metabolism Stem cells Transforming Growth Factor beta - metabolism Type 2 diabetes β-cell
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