Antiviral and Immunomodulatory Effects of Norantea brasiliensis Choisy on Dengue Virus-2
Background/Aims: Severe dengue fever is a result of exacerbated immune responses and no specific treatments are available. We evaluated the antiviral and immunomodulatory effects of Norantea brasiliensis Choisy. Methods: Human adherent monocytes infected in vitro with dengue virus (DENV)-2 were incu...
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| Veröffentlicht in: | Intervirology 2016-01, Vol.59 (4), p.217-227 |
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| creator | Fialho, Luciana Gomes da Silva, Vagner Pereira Reis, Sônia Regina Nogueira Ignácio Azeredo, Elzinandes Leal Kaplan, Maria Auxiliadora Coelho Figueiredo, Maria Raquel Kubelka, Claire Fernandes |
| description | Background/Aims: Severe dengue fever is a result of exacerbated immune responses and no specific treatments are available. We evaluated the antiviral and immunomodulatory effects of Norantea brasiliensis Choisy. Methods: Human adherent monocytes infected in vitro with dengue virus (DENV)-2 were incubated with the crude ethanol extract from leaves (NB1) or 3 derived fractions: dichloromethane (NB3), ethyl acetate (NB5), and butanolic (NB6) partitions. The antiviral and immunomodulatory activities were determined by intracellular detection of DENV antigen within monocytes and by secreted NS1 viral protein and cytokines. Results: The crude extract alone exhibited both antiviral activities (intracellular and secreted antigens) and all fractions derived from this extract modulated NS1 production. Regarding the immunomodulatory effect, among the secreted factors, TNF-α was inhibited by NB3 and NB6; IL-6 was inhibited by NB1, NB3, and NB6; IL-10 by NB1 and NB3; and IFN-α by NB6. The crude extract (NB1) presented the best antiviral effect, whereas the dichloromethane fraction (NB3) presented an immunomodulatory effect in the inflammatory and anti-inflammatory cytokines. Conclusion: During in vitro DENV infection, N. brasiliensis Choisy exerts both antiviral and immunomodulatory effects that are likely associated, considering that less viral load may lead to less immunostimulation. |
| doi_str_mv | 10.1159/000455855 |
| format | Article |
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We evaluated the antiviral and immunomodulatory effects of Norantea brasiliensis Choisy. Methods: Human adherent monocytes infected in vitro with dengue virus (DENV)-2 were incubated with the crude ethanol extract from leaves (NB1) or 3 derived fractions: dichloromethane (NB3), ethyl acetate (NB5), and butanolic (NB6) partitions. The antiviral and immunomodulatory activities were determined by intracellular detection of DENV antigen within monocytes and by secreted NS1 viral protein and cytokines. Results: The crude extract alone exhibited both antiviral activities (intracellular and secreted antigens) and all fractions derived from this extract modulated NS1 production. Regarding the immunomodulatory effect, among the secreted factors, TNF-α was inhibited by NB3 and NB6; IL-6 was inhibited by NB1, NB3, and NB6; IL-10 by NB1 and NB3; and IFN-α by NB6. The crude extract (NB1) presented the best antiviral effect, whereas the dichloromethane fraction (NB3) presented an immunomodulatory effect in the inflammatory and anti-inflammatory cytokines. Conclusion: During in vitro DENV infection, N. brasiliensis Choisy exerts both antiviral and immunomodulatory effects that are likely associated, considering that less viral load may lead to less immunostimulation.</description><identifier>ISSN: 0300-5526</identifier><identifier>EISSN: 1423-0100</identifier><identifier>DOI: 10.1159/000455855</identifier><identifier>PMID: 28329744</identifier><language>eng</language><publisher>Basel, Switzerland</publisher><subject>Antigens, Viral - analysis ; Antigens, Viral - immunology ; Antiviral Agents - chemistry ; Antiviral Agents - pharmacology ; Cytokines - antagonists & inhibitors ; Cytokines - drug effects ; Cytokines - immunology ; Cytokines - secretion ; Dengue Virus - drug effects ; Dengue Virus - immunology ; Ethanol - chemistry ; Humans ; Immunomodulation - drug effects ; In Vitro Techniques ; Interleukin-10 - antagonists & inhibitors ; Interleukin-6 - antagonists & inhibitors ; Magnoliopsida - chemistry ; Monocytes - drug effects ; Monocytes - immunology ; Monocytes - virology ; Original Paper ; Plant Extracts - chemistry ; Plant Extracts - pharmacology ; Plant Leaves - chemistry ; Plants, Medicinal - chemistry ; Tumor Necrosis Factor-alpha - antagonists & inhibitors ; Tumor Necrosis Factor-alpha - drug effects ; Viral Load - drug effects ; Viral Nonstructural Proteins - drug effects</subject><ispartof>Intervirology, 2016-01, Vol.59 (4), p.217-227</ispartof><rights>2017 S. Karger AG, Basel</rights><rights>2017 S. Karger AG, Basel.</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c341t-87ccebc86c819109cf0f9acfd6064507c89add7985a1fbf132609200252ac19d3</citedby><cites>FETCH-LOGICAL-c341t-87ccebc86c819109cf0f9acfd6064507c89add7985a1fbf132609200252ac19d3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,2429,27924,27925</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/28329744$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Fialho, Luciana Gomes</creatorcontrib><creatorcontrib>da Silva, Vagner Pereira</creatorcontrib><creatorcontrib>Reis, Sônia Regina Nogueira Ignácio</creatorcontrib><creatorcontrib>Azeredo, Elzinandes Leal</creatorcontrib><creatorcontrib>Kaplan, Maria Auxiliadora Coelho</creatorcontrib><creatorcontrib>Figueiredo, Maria Raquel</creatorcontrib><creatorcontrib>Kubelka, Claire Fernandes</creatorcontrib><title>Antiviral and Immunomodulatory Effects of Norantea brasiliensis Choisy on Dengue Virus-2</title><title>Intervirology</title><addtitle>Intervirology</addtitle><description>Background/Aims: Severe dengue fever is a result of exacerbated immune responses and no specific treatments are available. We evaluated the antiviral and immunomodulatory effects of Norantea brasiliensis Choisy. Methods: Human adherent monocytes infected in vitro with dengue virus (DENV)-2 were incubated with the crude ethanol extract from leaves (NB1) or 3 derived fractions: dichloromethane (NB3), ethyl acetate (NB5), and butanolic (NB6) partitions. The antiviral and immunomodulatory activities were determined by intracellular detection of DENV antigen within monocytes and by secreted NS1 viral protein and cytokines. Results: The crude extract alone exhibited both antiviral activities (intracellular and secreted antigens) and all fractions derived from this extract modulated NS1 production. Regarding the immunomodulatory effect, among the secreted factors, TNF-α was inhibited by NB3 and NB6; IL-6 was inhibited by NB1, NB3, and NB6; IL-10 by NB1 and NB3; and IFN-α by NB6. The crude extract (NB1) presented the best antiviral effect, whereas the dichloromethane fraction (NB3) presented an immunomodulatory effect in the inflammatory and anti-inflammatory cytokines. Conclusion: During in vitro DENV infection, N. brasiliensis Choisy exerts both antiviral and immunomodulatory effects that are likely associated, considering that less viral load may lead to less immunostimulation.</description><subject>Antigens, Viral - analysis</subject><subject>Antigens, Viral - immunology</subject><subject>Antiviral Agents - chemistry</subject><subject>Antiviral Agents - pharmacology</subject><subject>Cytokines - antagonists & inhibitors</subject><subject>Cytokines - drug effects</subject><subject>Cytokines - immunology</subject><subject>Cytokines - secretion</subject><subject>Dengue Virus - drug effects</subject><subject>Dengue Virus - immunology</subject><subject>Ethanol - chemistry</subject><subject>Humans</subject><subject>Immunomodulation - drug effects</subject><subject>In Vitro Techniques</subject><subject>Interleukin-10 - antagonists & inhibitors</subject><subject>Interleukin-6 - antagonists & inhibitors</subject><subject>Magnoliopsida - chemistry</subject><subject>Monocytes - drug effects</subject><subject>Monocytes - immunology</subject><subject>Monocytes - virology</subject><subject>Original Paper</subject><subject>Plant Extracts - chemistry</subject><subject>Plant Extracts - pharmacology</subject><subject>Plant Leaves - chemistry</subject><subject>Plants, Medicinal - chemistry</subject><subject>Tumor Necrosis Factor-alpha - antagonists & inhibitors</subject><subject>Tumor Necrosis Factor-alpha - drug effects</subject><subject>Viral Load - drug effects</subject><subject>Viral Nonstructural Proteins - drug effects</subject><issn>0300-5526</issn><issn>1423-0100</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2016</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNo90DFPwzAQhmELgWgpDOwIeYQhcLbjxB6rUqBSBQsgtshx7GJI4mInSP33BLV0uuW5b3gROidwQwiXtwCQci44P0BjklKWAAE4RGNgAAnnNBuhkxg_B8YIg2M0ooJRmafpGL1P2879uKBqrNoKL5qmb33jq75WnQ8bPLfW6C5ib_GTD6rtjMJlUNHVzrTRRTz78C5usG_xnWlXvcFvLvQxoafoyKo6mrPdnaDX-_nL7DFZPj8sZtNlollKukTkWptSi0wLIglIbcFKpW2VQZZyyLWQqqpyKbgitrSE0QwkBaCcKk1kxSboaru7Dv67N7ErGhe1qWvVGt_HgggBaSaA0YFeb6kOPsZgbLEOrlFhUxAo_kIW-5CDvdzN9mVjqr38LzeAiy34UmFlwh7s_n8BvVh2Pg</recordid><startdate>20160101</startdate><enddate>20160101</enddate><creator>Fialho, Luciana Gomes</creator><creator>da Silva, Vagner Pereira</creator><creator>Reis, Sônia Regina Nogueira Ignácio</creator><creator>Azeredo, Elzinandes Leal</creator><creator>Kaplan, Maria Auxiliadora Coelho</creator><creator>Figueiredo, Maria Raquel</creator><creator>Kubelka, Claire Fernandes</creator><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>20160101</creationdate><title>Antiviral and Immunomodulatory Effects of Norantea brasiliensis Choisy on Dengue Virus-2</title><author>Fialho, Luciana Gomes ; da Silva, Vagner Pereira ; Reis, Sônia Regina Nogueira Ignácio ; Azeredo, Elzinandes Leal ; Kaplan, Maria Auxiliadora Coelho ; Figueiredo, Maria Raquel ; Kubelka, Claire Fernandes</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c341t-87ccebc86c819109cf0f9acfd6064507c89add7985a1fbf132609200252ac19d3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2016</creationdate><topic>Antigens, Viral - analysis</topic><topic>Antigens, Viral - immunology</topic><topic>Antiviral Agents - chemistry</topic><topic>Antiviral Agents - pharmacology</topic><topic>Cytokines - antagonists & inhibitors</topic><topic>Cytokines - drug effects</topic><topic>Cytokines - immunology</topic><topic>Cytokines - secretion</topic><topic>Dengue Virus - drug effects</topic><topic>Dengue Virus - immunology</topic><topic>Ethanol - chemistry</topic><topic>Humans</topic><topic>Immunomodulation - drug effects</topic><topic>In Vitro Techniques</topic><topic>Interleukin-10 - antagonists & inhibitors</topic><topic>Interleukin-6 - antagonists & inhibitors</topic><topic>Magnoliopsida - chemistry</topic><topic>Monocytes - drug effects</topic><topic>Monocytes - immunology</topic><topic>Monocytes - virology</topic><topic>Original Paper</topic><topic>Plant Extracts - chemistry</topic><topic>Plant Extracts - pharmacology</topic><topic>Plant Leaves - chemistry</topic><topic>Plants, Medicinal - chemistry</topic><topic>Tumor Necrosis Factor-alpha - antagonists & inhibitors</topic><topic>Tumor Necrosis Factor-alpha - drug effects</topic><topic>Viral Load - drug effects</topic><topic>Viral Nonstructural Proteins - drug effects</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Fialho, Luciana Gomes</creatorcontrib><creatorcontrib>da Silva, Vagner Pereira</creatorcontrib><creatorcontrib>Reis, Sônia Regina Nogueira Ignácio</creatorcontrib><creatorcontrib>Azeredo, Elzinandes Leal</creatorcontrib><creatorcontrib>Kaplan, Maria Auxiliadora Coelho</creatorcontrib><creatorcontrib>Figueiredo, Maria Raquel</creatorcontrib><creatorcontrib>Kubelka, Claire Fernandes</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Intervirology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Fialho, Luciana Gomes</au><au>da Silva, Vagner Pereira</au><au>Reis, Sônia Regina Nogueira Ignácio</au><au>Azeredo, Elzinandes Leal</au><au>Kaplan, Maria Auxiliadora Coelho</au><au>Figueiredo, Maria Raquel</au><au>Kubelka, Claire Fernandes</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Antiviral and Immunomodulatory Effects of Norantea brasiliensis Choisy on Dengue Virus-2</atitle><jtitle>Intervirology</jtitle><addtitle>Intervirology</addtitle><date>2016-01-01</date><risdate>2016</risdate><volume>59</volume><issue>4</issue><spage>217</spage><epage>227</epage><pages>217-227</pages><issn>0300-5526</issn><eissn>1423-0100</eissn><abstract>Background/Aims: Severe dengue fever is a result of exacerbated immune responses and no specific treatments are available. We evaluated the antiviral and immunomodulatory effects of Norantea brasiliensis Choisy. Methods: Human adherent monocytes infected in vitro with dengue virus (DENV)-2 were incubated with the crude ethanol extract from leaves (NB1) or 3 derived fractions: dichloromethane (NB3), ethyl acetate (NB5), and butanolic (NB6) partitions. The antiviral and immunomodulatory activities were determined by intracellular detection of DENV antigen within monocytes and by secreted NS1 viral protein and cytokines. Results: The crude extract alone exhibited both antiviral activities (intracellular and secreted antigens) and all fractions derived from this extract modulated NS1 production. Regarding the immunomodulatory effect, among the secreted factors, TNF-α was inhibited by NB3 and NB6; IL-6 was inhibited by NB1, NB3, and NB6; IL-10 by NB1 and NB3; and IFN-α by NB6. The crude extract (NB1) presented the best antiviral effect, whereas the dichloromethane fraction (NB3) presented an immunomodulatory effect in the inflammatory and anti-inflammatory cytokines. Conclusion: During in vitro DENV infection, N. brasiliensis Choisy exerts both antiviral and immunomodulatory effects that are likely associated, considering that less viral load may lead to less immunostimulation.</abstract><cop>Basel, Switzerland</cop><pmid>28329744</pmid><doi>10.1159/000455855</doi><tpages>11</tpages><oa>free_for_read</oa></addata></record> |
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| subjects | Antigens, Viral - analysis Antigens, Viral - immunology Antiviral Agents - chemistry Antiviral Agents - pharmacology Cytokines - antagonists & inhibitors Cytokines - drug effects Cytokines - immunology Cytokines - secretion Dengue Virus - drug effects Dengue Virus - immunology Ethanol - chemistry Humans Immunomodulation - drug effects In Vitro Techniques Interleukin-10 - antagonists & inhibitors Interleukin-6 - antagonists & inhibitors Magnoliopsida - chemistry Monocytes - drug effects Monocytes - immunology Monocytes - virology Original Paper Plant Extracts - chemistry Plant Extracts - pharmacology Plant Leaves - chemistry Plants, Medicinal - chemistry Tumor Necrosis Factor-alpha - antagonists & inhibitors Tumor Necrosis Factor-alpha - drug effects Viral Load - drug effects Viral Nonstructural Proteins - drug effects |
| title | Antiviral and Immunomodulatory Effects of Norantea brasiliensis Choisy on Dengue Virus-2 |
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