HOX Antisense lincRNA HOXA-AS2 Promotes Tumorigenesis of Hepatocellular Carcinoma
Background: Recent studies reveal that long non-coding RNAs (LncRNAs) play critical roles in the proliferation and migration of human cancer. Previous report has shown that LncRNA HOXA-AS2 was involved in carcinoma processes. However, the expression and biological function of HOXA-AS2 in hepatocellu...
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| Veröffentlicht in: | Cellular physiology and biochemistry 2016-01, Vol.40 (1-2), p.287-296 |
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| creator | Wang, Fuqiang Yang, Huili Deng, Zhigang Su, Yongjie Fang, QinLiang Yin, Zhenyu |
| description | Background: Recent studies reveal that long non-coding RNAs (LncRNAs) play critical roles in the proliferation and migration of human cancer. Previous report has shown that LncRNA HOXA-AS2 was involved in carcinoma processes. However, the expression and biological function of HOXA-AS2 in hepatocellular carcinoma (HCC) are poorly understood. Methods: Quantitative real-time PCR (qRT-PCR) was performed to detect the expression of HOXA-AS2 in HCC tissues and cell lines. The relation between lncRNA HOXA-AS2 expression and clinicopathological characteristics was assessed by chi-square test. The prognosis was analyzed using Kaplan-Meier method, and compared differences between the two groups by log-rank test. The biological function of HOXA-AS2 on HCC cells were determined both in vitro and in vivo. Results: In the present study, we found that HOXA-AS2 expression was increased in HCC tissues and adjacent normal tissues and high HOXA-AS2 expression was associated with bigger tumor size, advanced tumor stage, and shorter survival time. Knockdown of HOXA-AS2 significantly inhibited HCC cell proliferation and invasion and resulted in an increase of apoptosis. Furthermore, inhibition of HOXA-AS2 in HCC cells significantly repressed tumorigenicity in nude mice. Conclusion: Our results indicated that the inhibition of HOXA-AS2 in HCC cells significantly inhibited cell proliferation in vitro and in vivo, which might provide a potential possibility for targeted therapy of HCC. |
| doi_str_mv | 10.1159/000452545 |
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Previous report has shown that LncRNA HOXA-AS2 was involved in carcinoma processes. However, the expression and biological function of HOXA-AS2 in hepatocellular carcinoma (HCC) are poorly understood. Methods: Quantitative real-time PCR (qRT-PCR) was performed to detect the expression of HOXA-AS2 in HCC tissues and cell lines. The relation between lncRNA HOXA-AS2 expression and clinicopathological characteristics was assessed by chi-square test. The prognosis was analyzed using Kaplan-Meier method, and compared differences between the two groups by log-rank test. The biological function of HOXA-AS2 on HCC cells were determined both in vitro and in vivo. Results: In the present study, we found that HOXA-AS2 expression was increased in HCC tissues and adjacent normal tissues and high HOXA-AS2 expression was associated with bigger tumor size, advanced tumor stage, and shorter survival time. Knockdown of HOXA-AS2 significantly inhibited HCC cell proliferation and invasion and resulted in an increase of apoptosis. Furthermore, inhibition of HOXA-AS2 in HCC cells significantly repressed tumorigenicity in nude mice. Conclusion: Our results indicated that the inhibition of HOXA-AS2 in HCC cells significantly inhibited cell proliferation in vitro and in vivo, which might provide a potential possibility for targeted therapy of HCC.</description><identifier>ISSN: 1015-8987</identifier><identifier>EISSN: 1421-9778</identifier><identifier>DOI: 10.1159/000452545</identifier><identifier>PMID: 27855366</identifier><language>eng</language><publisher>Basel, Switzerland: Cell Physiol Biochem Press GmbH & Co KG</publisher><subject>Animals ; Carcinogenesis - genetics ; Carcinogenesis - pathology ; Carcinoma, Hepatocellular - genetics ; Carcinoma, Hepatocellular - pathology ; Cell Line, Tumor ; Cell migration ; Cell Movement - genetics ; Cell Proliferation ; Clone Cells ; Female ; Gene Expression Regulation, Neoplastic ; Gene Knockdown Techniques ; Gene Silencing ; Hepatocellular carcinoma ; HOXA-AS2 ; Humans ; Kaplan-Meier Estimate ; Liver Neoplasms - genetics ; Liver Neoplasms - pathology ; Male ; Mice ; Mice, Inbred BALB C ; Mice, Nude ; Middle Aged ; Neoplasm Invasiveness ; Original Paper ; Prognosis ; RNA, Long Noncoding - genetics ; RNA, Long Noncoding - metabolism ; Up-Regulation - genetics ; Xenograft Model Antitumor Assays</subject><ispartof>Cellular physiology and biochemistry, 2016-01, Vol.40 (1-2), p.287-296</ispartof><rights>2016 The Author(s) Published by S. Karger AG, Basel</rights><rights>2016 The Author(s) Published by S. Karger AG, Basel.</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c435t-981eb7665d4704851ef96b55c630f25f4df1fba696db59ebc44f755fbd2afded3</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,864,2102,27635,27924,27925</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/27855366$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Wang, Fuqiang</creatorcontrib><creatorcontrib>Yang, Huili</creatorcontrib><creatorcontrib>Deng, Zhigang</creatorcontrib><creatorcontrib>Su, Yongjie</creatorcontrib><creatorcontrib>Fang, QinLiang</creatorcontrib><creatorcontrib>Yin, Zhenyu</creatorcontrib><title>HOX Antisense lincRNA HOXA-AS2 Promotes Tumorigenesis of Hepatocellular Carcinoma</title><title>Cellular physiology and biochemistry</title><addtitle>Cell Physiol Biochem</addtitle><description>Background: Recent studies reveal that long non-coding RNAs (LncRNAs) play critical roles in the proliferation and migration of human cancer. Previous report has shown that LncRNA HOXA-AS2 was involved in carcinoma processes. However, the expression and biological function of HOXA-AS2 in hepatocellular carcinoma (HCC) are poorly understood. Methods: Quantitative real-time PCR (qRT-PCR) was performed to detect the expression of HOXA-AS2 in HCC tissues and cell lines. The relation between lncRNA HOXA-AS2 expression and clinicopathological characteristics was assessed by chi-square test. The prognosis was analyzed using Kaplan-Meier method, and compared differences between the two groups by log-rank test. The biological function of HOXA-AS2 on HCC cells were determined both in vitro and in vivo. Results: In the present study, we found that HOXA-AS2 expression was increased in HCC tissues and adjacent normal tissues and high HOXA-AS2 expression was associated with bigger tumor size, advanced tumor stage, and shorter survival time. Knockdown of HOXA-AS2 significantly inhibited HCC cell proliferation and invasion and resulted in an increase of apoptosis. Furthermore, inhibition of HOXA-AS2 in HCC cells significantly repressed tumorigenicity in nude mice. Conclusion: Our results indicated that the inhibition of HOXA-AS2 in HCC cells significantly inhibited cell proliferation in vitro and in vivo, which might provide a potential possibility for targeted therapy of HCC.</description><subject>Animals</subject><subject>Carcinogenesis - genetics</subject><subject>Carcinogenesis - pathology</subject><subject>Carcinoma, Hepatocellular - genetics</subject><subject>Carcinoma, Hepatocellular - pathology</subject><subject>Cell Line, Tumor</subject><subject>Cell migration</subject><subject>Cell Movement - genetics</subject><subject>Cell Proliferation</subject><subject>Clone Cells</subject><subject>Female</subject><subject>Gene Expression Regulation, Neoplastic</subject><subject>Gene Knockdown Techniques</subject><subject>Gene Silencing</subject><subject>Hepatocellular carcinoma</subject><subject>HOXA-AS2</subject><subject>Humans</subject><subject>Kaplan-Meier Estimate</subject><subject>Liver Neoplasms - genetics</subject><subject>Liver Neoplasms - pathology</subject><subject>Male</subject><subject>Mice</subject><subject>Mice, Inbred BALB C</subject><subject>Mice, Nude</subject><subject>Middle Aged</subject><subject>Neoplasm Invasiveness</subject><subject>Original Paper</subject><subject>Prognosis</subject><subject>RNA, Long Noncoding - genetics</subject><subject>RNA, Long Noncoding - metabolism</subject><subject>Up-Regulation - genetics</subject><subject>Xenograft Model Antitumor Assays</subject><issn>1015-8987</issn><issn>1421-9778</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2016</creationdate><recordtype>article</recordtype><sourceid>M--</sourceid><sourceid>EIF</sourceid><sourceid>DOA</sourceid><recordid>eNptkb9v1TAQxyMEoqUwsCMUiQWGgO347HgMT7SvUkULFInNcuzzk0sSP-xk4L8nJSUT0_363Pd0d0XxkpL3lIL6QAjhwIDDo-KUckYrJWXzePEJhapRjTwpnuV8R5ZQKva0OGGyAaiFOC2-7K9_lO04hYxjxrIPo_36uS2XbFu131h5k-IQJ8zl7TzEFA44Yg65jL7c49FM0WLfz71J5c4kG8Y4mOfFE2_6jC8e7Fnx_fzT7W5fXV1fXO7aq8ryGqZKNRQ7KQQ4LglvgKJXogOwoiaegefOU98ZoYTrQGFnOfcSwHeOGe_Q1WfF5arrornTxxQGk37raIL-m4jpoE2agu1Rc89BLrtLyig3BgxzjKATlnZNp7xYtN6uWscUf82YJz2EfL-aGTHOWdOGU6mg4XJB362oTTHnhH4bTYm-_4bevrGwrx9k525At5H_zr8Ar1bgp0kHTBuw9b_5b3l383El9NH5-g_Ru5gE</recordid><startdate>20160101</startdate><enddate>20160101</enddate><creator>Wang, Fuqiang</creator><creator>Yang, Huili</creator><creator>Deng, Zhigang</creator><creator>Su, Yongjie</creator><creator>Fang, QinLiang</creator><creator>Yin, Zhenyu</creator><general>Cell Physiol Biochem Press GmbH & Co KG</general><scope>M--</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><scope>DOA</scope></search><sort><creationdate>20160101</creationdate><title>HOX Antisense lincRNA HOXA-AS2 Promotes Tumorigenesis of Hepatocellular Carcinoma</title><author>Wang, Fuqiang ; Yang, Huili ; Deng, Zhigang ; Su, Yongjie ; Fang, QinLiang ; Yin, Zhenyu</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c435t-981eb7665d4704851ef96b55c630f25f4df1fba696db59ebc44f755fbd2afded3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2016</creationdate><topic>Animals</topic><topic>Carcinogenesis - genetics</topic><topic>Carcinogenesis - pathology</topic><topic>Carcinoma, Hepatocellular - genetics</topic><topic>Carcinoma, Hepatocellular - pathology</topic><topic>Cell Line, Tumor</topic><topic>Cell migration</topic><topic>Cell Movement - genetics</topic><topic>Cell Proliferation</topic><topic>Clone Cells</topic><topic>Female</topic><topic>Gene Expression Regulation, Neoplastic</topic><topic>Gene Knockdown Techniques</topic><topic>Gene Silencing</topic><topic>Hepatocellular carcinoma</topic><topic>HOXA-AS2</topic><topic>Humans</topic><topic>Kaplan-Meier Estimate</topic><topic>Liver Neoplasms - genetics</topic><topic>Liver Neoplasms - pathology</topic><topic>Male</topic><topic>Mice</topic><topic>Mice, Inbred BALB C</topic><topic>Mice, Nude</topic><topic>Middle Aged</topic><topic>Neoplasm Invasiveness</topic><topic>Original Paper</topic><topic>Prognosis</topic><topic>RNA, Long Noncoding - genetics</topic><topic>RNA, Long Noncoding - metabolism</topic><topic>Up-Regulation - genetics</topic><topic>Xenograft Model Antitumor Assays</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Wang, Fuqiang</creatorcontrib><creatorcontrib>Yang, Huili</creatorcontrib><creatorcontrib>Deng, Zhigang</creatorcontrib><creatorcontrib>Su, Yongjie</creatorcontrib><creatorcontrib>Fang, QinLiang</creatorcontrib><creatorcontrib>Yin, Zhenyu</creatorcontrib><collection>Karger Open Access</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><collection>DOAJ Directory of Open Access Journals</collection><jtitle>Cellular physiology and biochemistry</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Wang, Fuqiang</au><au>Yang, Huili</au><au>Deng, Zhigang</au><au>Su, Yongjie</au><au>Fang, QinLiang</au><au>Yin, Zhenyu</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>HOX Antisense lincRNA HOXA-AS2 Promotes Tumorigenesis of Hepatocellular Carcinoma</atitle><jtitle>Cellular physiology and biochemistry</jtitle><addtitle>Cell Physiol Biochem</addtitle><date>2016-01-01</date><risdate>2016</risdate><volume>40</volume><issue>1-2</issue><spage>287</spage><epage>296</epage><pages>287-296</pages><issn>1015-8987</issn><eissn>1421-9778</eissn><abstract>Background: Recent studies reveal that long non-coding RNAs (LncRNAs) play critical roles in the proliferation and migration of human cancer. Previous report has shown that LncRNA HOXA-AS2 was involved in carcinoma processes. However, the expression and biological function of HOXA-AS2 in hepatocellular carcinoma (HCC) are poorly understood. Methods: Quantitative real-time PCR (qRT-PCR) was performed to detect the expression of HOXA-AS2 in HCC tissues and cell lines. The relation between lncRNA HOXA-AS2 expression and clinicopathological characteristics was assessed by chi-square test. The prognosis was analyzed using Kaplan-Meier method, and compared differences between the two groups by log-rank test. The biological function of HOXA-AS2 on HCC cells were determined both in vitro and in vivo. Results: In the present study, we found that HOXA-AS2 expression was increased in HCC tissues and adjacent normal tissues and high HOXA-AS2 expression was associated with bigger tumor size, advanced tumor stage, and shorter survival time. Knockdown of HOXA-AS2 significantly inhibited HCC cell proliferation and invasion and resulted in an increase of apoptosis. Furthermore, inhibition of HOXA-AS2 in HCC cells significantly repressed tumorigenicity in nude mice. Conclusion: Our results indicated that the inhibition of HOXA-AS2 in HCC cells significantly inhibited cell proliferation in vitro and in vivo, which might provide a potential possibility for targeted therapy of HCC.</abstract><cop>Basel, Switzerland</cop><pub>Cell Physiol Biochem Press GmbH & Co KG</pub><pmid>27855366</pmid><doi>10.1159/000452545</doi><tpages>10</tpages><oa>free_for_read</oa></addata></record> |
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| subjects | Animals Carcinogenesis - genetics Carcinogenesis - pathology Carcinoma, Hepatocellular - genetics Carcinoma, Hepatocellular - pathology Cell Line, Tumor Cell migration Cell Movement - genetics Cell Proliferation Clone Cells Female Gene Expression Regulation, Neoplastic Gene Knockdown Techniques Gene Silencing Hepatocellular carcinoma HOXA-AS2 Humans Kaplan-Meier Estimate Liver Neoplasms - genetics Liver Neoplasms - pathology Male Mice Mice, Inbred BALB C Mice, Nude Middle Aged Neoplasm Invasiveness Original Paper Prognosis RNA, Long Noncoding - genetics RNA, Long Noncoding - metabolism Up-Regulation - genetics Xenograft Model Antitumor Assays |
| title | HOX Antisense lincRNA HOXA-AS2 Promotes Tumorigenesis of Hepatocellular Carcinoma |
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