Effect of an Experimental Paste with Hydroxyapatite Nanoparticles and Fluoride on Dental Demineralisation and Remineralisation in situ
This study evaluated the effect of an experimental paste containing hydroxyapatite in nanoparticles (nano-HA)/fluoride on dental de-remineralisation in situ. Thirteen subjects took part in this crossover/randomised/double-blind study performed in 4 phases (14 days each). Four sound and 4 pre-deminer...
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| Veröffentlicht in: | Caries research 2015-01, Vol.49 (5), p.499-507 |
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| creator | Souza, Beatriz M. Comar, Livia P. Vertuan, Mariele Fernandes Neto, Constantino Buzalaf, Marília Afonso Rabelo Magalhães, Ana Carolina |
| description | This study evaluated the effect of an experimental paste containing hydroxyapatite in nanoparticles (nano-HA)/fluoride on dental de-remineralisation in situ. Thirteen subjects took part in this crossover/randomised/double-blind study performed in 4 phases (14 days each). Four sound and 4 pre-demineralised specimens were worn intraorally at each phase corresponding to the following treatments: Nanop Plus (10% HA, 0.2% NaF, nano-HA/fluoride), MI Paste Plus (casein phosphopeptide-amorphous calcium phosphate, 0.2% NaF), F (0.2% NaF) and placebo. Two-hundred and forty enamel and 240 dentine specimens were selected by using surface microhardness; half of them were subjected to pre-demineralisation and the other half remained sound. Sound specimens were further exposed to severe cariogenic challenge (20% sucrose in biofilm) in situ, while pre-demineralised specimens were not. All specimens were exposed to fluoride dentifrice slurry 2 × 1 min/day. Thereafter, the treatments were done for 4 min. The de-remineralisation was quantified by transversal microradiography. The data were statistically analysed by repeated-measures ANOVA/Tukey's tests (p < 0.05). Generally, no huge differences were found among the treatments. However, Nanop Plus was the only treatment able to significantly reduce dentine demineralisation (ΔZ, integrated mineral loss) and to improve enamel remineralisation (ΔΔZ, integrated mineral uptake) compared to placebo. No treatments were able to reduce enamel demineralisation, while for dentine remineralisation all treatments were similarly effective in improving ΔΔZ compared to placebo. Nanop Plus seems to have a positive influence on dental de-remineralisation, which should be further confirmed. |
| doi_str_mv | 10.1159/000438466 |
| format | Article |
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Thirteen subjects took part in this crossover/randomised/double-blind study performed in 4 phases (14 days each). Four sound and 4 pre-demineralised specimens were worn intraorally at each phase corresponding to the following treatments: Nanop Plus (10% HA, 0.2% NaF, nano-HA/fluoride), MI Paste Plus (casein phosphopeptide-amorphous calcium phosphate, 0.2% NaF), F (0.2% NaF) and placebo. Two-hundred and forty enamel and 240 dentine specimens were selected by using surface microhardness; half of them were subjected to pre-demineralisation and the other half remained sound. Sound specimens were further exposed to severe cariogenic challenge (20% sucrose in biofilm) in situ, while pre-demineralised specimens were not. All specimens were exposed to fluoride dentifrice slurry 2 × 1 min/day. Thereafter, the treatments were done for 4 min. The de-remineralisation was quantified by transversal microradiography. The data were statistically analysed by repeated-measures ANOVA/Tukey's tests (p < 0.05). Generally, no huge differences were found among the treatments. However, Nanop Plus was the only treatment able to significantly reduce dentine demineralisation (ΔZ, integrated mineral loss) and to improve enamel remineralisation (ΔΔZ, integrated mineral uptake) compared to placebo. No treatments were able to reduce enamel demineralisation, while for dentine remineralisation all treatments were similarly effective in improving ΔΔZ compared to placebo. Nanop Plus seems to have a positive influence on dental de-remineralisation, which should be further confirmed.</description><identifier>ISSN: 0008-6568</identifier><identifier>EISSN: 1421-976X</identifier><identifier>DOI: 10.1159/000438466</identifier><identifier>PMID: 26278685</identifier><identifier>CODEN: CAREBK</identifier><language>eng</language><publisher>Basel, Switzerland: S. Karger AG</publisher><subject><![CDATA[Adult ; Calcium Phosphates - administration & dosage ; Cariostatic Agents - therapeutic use ; Caseins - administration & dosage ; Cross-Over Studies ; Dental Enamel - diagnostic imaging ; Dental Enamel - drug effects ; Dentifrices - chemistry ; Dentin - diagnostic imaging ; Dentin - drug effects ; Dentistry ; Double-Blind Method ; Durapatite - administration & dosage ; Durapatite - pharmacology ; Female ; Fluorides ; Humans ; Male ; Microradiography ; Nanoparticles - administration & dosage ; Original Paper ; Random Allocation ; Sodium Fluoride - administration & dosage ; Tooth Demineralization - prevention & control ; Tooth Remineralization - methods ; Toothpastes - chemistry ; Toothpastes - pharmacology ; Young Adult]]></subject><ispartof>Caries research, 2015-01, Vol.49 (5), p.499-507</ispartof><rights>2015 S. Karger AG, Basel</rights><rights>2015 S. Karger AG, Basel.</rights><rights>Copyright S. Karger AG Jan 2015</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c367t-4151365b7340794c3a78555a392922db0fc1face836a045cd7471d31202fece23</citedby><orcidid>0000-0002-5985-3951</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,2429,27924,27925</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/26278685$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Souza, Beatriz M.</creatorcontrib><creatorcontrib>Comar, Livia P.</creatorcontrib><creatorcontrib>Vertuan, Mariele</creatorcontrib><creatorcontrib>Fernandes Neto, Constantino</creatorcontrib><creatorcontrib>Buzalaf, Marília Afonso Rabelo</creatorcontrib><creatorcontrib>Magalhães, Ana Carolina</creatorcontrib><title>Effect of an Experimental Paste with Hydroxyapatite Nanoparticles and Fluoride on Dental Demineralisation and Remineralisation in situ</title><title>Caries research</title><addtitle>Caries Res</addtitle><description>This study evaluated the effect of an experimental paste containing hydroxyapatite in nanoparticles (nano-HA)/fluoride on dental de-remineralisation in situ. Thirteen subjects took part in this crossover/randomised/double-blind study performed in 4 phases (14 days each). Four sound and 4 pre-demineralised specimens were worn intraorally at each phase corresponding to the following treatments: Nanop Plus (10% HA, 0.2% NaF, nano-HA/fluoride), MI Paste Plus (casein phosphopeptide-amorphous calcium phosphate, 0.2% NaF), F (0.2% NaF) and placebo. Two-hundred and forty enamel and 240 dentine specimens were selected by using surface microhardness; half of them were subjected to pre-demineralisation and the other half remained sound. Sound specimens were further exposed to severe cariogenic challenge (20% sucrose in biofilm) in situ, while pre-demineralised specimens were not. All specimens were exposed to fluoride dentifrice slurry 2 × 1 min/day. Thereafter, the treatments were done for 4 min. The de-remineralisation was quantified by transversal microradiography. The data were statistically analysed by repeated-measures ANOVA/Tukey's tests (p < 0.05). Generally, no huge differences were found among the treatments. However, Nanop Plus was the only treatment able to significantly reduce dentine demineralisation (ΔZ, integrated mineral loss) and to improve enamel remineralisation (ΔΔZ, integrated mineral uptake) compared to placebo. No treatments were able to reduce enamel demineralisation, while for dentine remineralisation all treatments were similarly effective in improving ΔΔZ compared to placebo. Nanop Plus seems to have a positive influence on dental de-remineralisation, which should be further confirmed.</description><subject>Adult</subject><subject>Calcium Phosphates - administration & dosage</subject><subject>Cariostatic Agents - therapeutic use</subject><subject>Caseins - administration & dosage</subject><subject>Cross-Over Studies</subject><subject>Dental Enamel - diagnostic imaging</subject><subject>Dental Enamel - drug effects</subject><subject>Dentifrices - chemistry</subject><subject>Dentin - diagnostic imaging</subject><subject>Dentin - drug effects</subject><subject>Dentistry</subject><subject>Double-Blind Method</subject><subject>Durapatite - administration & dosage</subject><subject>Durapatite - pharmacology</subject><subject>Female</subject><subject>Fluorides</subject><subject>Humans</subject><subject>Male</subject><subject>Microradiography</subject><subject>Nanoparticles - administration & dosage</subject><subject>Original Paper</subject><subject>Random Allocation</subject><subject>Sodium Fluoride - administration & dosage</subject><subject>Tooth Demineralization - prevention & control</subject><subject>Tooth Remineralization - methods</subject><subject>Toothpastes - chemistry</subject><subject>Toothpastes - pharmacology</subject><subject>Young Adult</subject><issn>0008-6568</issn><issn>1421-976X</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2015</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><recordid>eNqN0U1LHDEYB_Agim7XHrxLCXiph9G8TF7mWHTtCmKLtNDbkM08o7Gzk2mSQfcL9HMb2XUPnjwF_vz-Dzx5EDqi5IxSUZ0TQkquSyl30ISWjBaVkn920STnupBC6gP0KcZHQmg2eh8dMMmUllpM0P9Z24JN2LfY9Hj2PEBwS-iT6fBPExPgJ5ce8HzVBP-8MoNJLme3pveDCcnZDmLuNfiqG31wDWDf48t1_RKWrodgOhdzK-ev7u596HocXRoP0V5rugifN-8U_b6a_bqYFzc_vl9ffLspLJcqFSUVlEuxULwkqiotN0oLIQyvWMVYsyCtpa2xoLk0pBS2UaWiDaeMsLwlMD5FX9dzh-D_jRBTvXTRQteZHvwYa6qUzF_EdfUByrRUnHKd6ck7-ujH0OdFspI8X0ErldXpWtngYwzQ1kP-axNWNSX16x3r7R2z_bKZOC6W0Gzl2-EyOF6DvybcQ9iCTf8Fb4ihDg</recordid><startdate>20150101</startdate><enddate>20150101</enddate><creator>Souza, Beatriz M.</creator><creator>Comar, Livia P.</creator><creator>Vertuan, Mariele</creator><creator>Fernandes Neto, Constantino</creator><creator>Buzalaf, Marília Afonso Rabelo</creator><creator>Magalhães, Ana Carolina</creator><general>S. Karger AG</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7QL</scope><scope>7QO</scope><scope>7QP</scope><scope>7RV</scope><scope>7U7</scope><scope>7X7</scope><scope>7XB</scope><scope>88E</scope><scope>8AO</scope><scope>8FD</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>BENPR</scope><scope>C1K</scope><scope>CCPQU</scope><scope>FR3</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>K9.</scope><scope>KB0</scope><scope>M0S</scope><scope>M1P</scope><scope>NAPCQ</scope><scope>P64</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>RC3</scope><scope>S0X</scope><scope>7X8</scope><orcidid>https://orcid.org/0000-0002-5985-3951</orcidid></search><sort><creationdate>20150101</creationdate><title>Effect of an Experimental Paste with Hydroxyapatite Nanoparticles and Fluoride on Dental Demineralisation and Remineralisation in situ</title><author>Souza, Beatriz M. ; Comar, Livia P. ; Vertuan, Mariele ; Fernandes Neto, Constantino ; Buzalaf, Marília Afonso Rabelo ; Magalhães, Ana Carolina</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c367t-4151365b7340794c3a78555a392922db0fc1face836a045cd7471d31202fece23</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2015</creationdate><topic>Adult</topic><topic>Calcium Phosphates - administration & dosage</topic><topic>Cariostatic Agents - therapeutic use</topic><topic>Caseins - administration & dosage</topic><topic>Cross-Over Studies</topic><topic>Dental Enamel - diagnostic imaging</topic><topic>Dental Enamel - drug effects</topic><topic>Dentifrices - chemistry</topic><topic>Dentin - diagnostic imaging</topic><topic>Dentin - drug effects</topic><topic>Dentistry</topic><topic>Double-Blind Method</topic><topic>Durapatite - administration & dosage</topic><topic>Durapatite - pharmacology</topic><topic>Female</topic><topic>Fluorides</topic><topic>Humans</topic><topic>Male</topic><topic>Microradiography</topic><topic>Nanoparticles - administration & dosage</topic><topic>Original Paper</topic><topic>Random Allocation</topic><topic>Sodium Fluoride - administration & dosage</topic><topic>Tooth Demineralization - prevention & control</topic><topic>Tooth Remineralization - methods</topic><topic>Toothpastes - chemistry</topic><topic>Toothpastes - pharmacology</topic><topic>Young Adult</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Souza, Beatriz M.</creatorcontrib><creatorcontrib>Comar, Livia P.</creatorcontrib><creatorcontrib>Vertuan, Mariele</creatorcontrib><creatorcontrib>Fernandes Neto, Constantino</creatorcontrib><creatorcontrib>Buzalaf, Marília Afonso Rabelo</creatorcontrib><creatorcontrib>Magalhães, Ana Carolina</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Biotechnology Research Abstracts</collection><collection>Calcium & Calcified Tissue Abstracts</collection><collection>Proquest Nursing & Allied Health Source</collection><collection>Toxicology Abstracts</collection><collection>Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Medical Database (Alumni Edition)</collection><collection>ProQuest Pharma Collection</collection><collection>Technology Research Database</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest Central UK/Ireland</collection><collection>ProQuest Central</collection><collection>Environmental Sciences and Pollution Management</collection><collection>ProQuest One Community College</collection><collection>Engineering Research Database</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>Nursing & Allied Health Database (Alumni Edition)</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Medical Database</collection><collection>Nursing & Allied Health Premium</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central China</collection><collection>Genetics Abstracts</collection><collection>SIRS Editorial</collection><collection>MEDLINE - Academic</collection><jtitle>Caries research</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Souza, Beatriz M.</au><au>Comar, Livia P.</au><au>Vertuan, Mariele</au><au>Fernandes Neto, Constantino</au><au>Buzalaf, Marília Afonso Rabelo</au><au>Magalhães, Ana Carolina</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Effect of an Experimental Paste with Hydroxyapatite Nanoparticles and Fluoride on Dental Demineralisation and Remineralisation in situ</atitle><jtitle>Caries research</jtitle><addtitle>Caries Res</addtitle><date>2015-01-01</date><risdate>2015</risdate><volume>49</volume><issue>5</issue><spage>499</spage><epage>507</epage><pages>499-507</pages><issn>0008-6568</issn><eissn>1421-976X</eissn><coden>CAREBK</coden><abstract>This study evaluated the effect of an experimental paste containing hydroxyapatite in nanoparticles (nano-HA)/fluoride on dental de-remineralisation in situ. Thirteen subjects took part in this crossover/randomised/double-blind study performed in 4 phases (14 days each). Four sound and 4 pre-demineralised specimens were worn intraorally at each phase corresponding to the following treatments: Nanop Plus (10% HA, 0.2% NaF, nano-HA/fluoride), MI Paste Plus (casein phosphopeptide-amorphous calcium phosphate, 0.2% NaF), F (0.2% NaF) and placebo. Two-hundred and forty enamel and 240 dentine specimens were selected by using surface microhardness; half of them were subjected to pre-demineralisation and the other half remained sound. Sound specimens were further exposed to severe cariogenic challenge (20% sucrose in biofilm) in situ, while pre-demineralised specimens were not. All specimens were exposed to fluoride dentifrice slurry 2 × 1 min/day. Thereafter, the treatments were done for 4 min. The de-remineralisation was quantified by transversal microradiography. The data were statistically analysed by repeated-measures ANOVA/Tukey's tests (p < 0.05). Generally, no huge differences were found among the treatments. However, Nanop Plus was the only treatment able to significantly reduce dentine demineralisation (ΔZ, integrated mineral loss) and to improve enamel remineralisation (ΔΔZ, integrated mineral uptake) compared to placebo. No treatments were able to reduce enamel demineralisation, while for dentine remineralisation all treatments were similarly effective in improving ΔΔZ compared to placebo. Nanop Plus seems to have a positive influence on dental de-remineralisation, which should be further confirmed.</abstract><cop>Basel, Switzerland</cop><pub>S. Karger AG</pub><pmid>26278685</pmid><doi>10.1159/000438466</doi><tpages>9</tpages><orcidid>https://orcid.org/0000-0002-5985-3951</orcidid></addata></record> |
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| source | MEDLINE; Karger Journals |
| subjects | Adult Calcium Phosphates - administration & dosage Cariostatic Agents - therapeutic use Caseins - administration & dosage Cross-Over Studies Dental Enamel - diagnostic imaging Dental Enamel - drug effects Dentifrices - chemistry Dentin - diagnostic imaging Dentin - drug effects Dentistry Double-Blind Method Durapatite - administration & dosage Durapatite - pharmacology Female Fluorides Humans Male Microradiography Nanoparticles - administration & dosage Original Paper Random Allocation Sodium Fluoride - administration & dosage Tooth Demineralization - prevention & control Tooth Remineralization - methods Toothpastes - chemistry Toothpastes - pharmacology Young Adult |
| title | Effect of an Experimental Paste with Hydroxyapatite Nanoparticles and Fluoride on Dental Demineralisation and Remineralisation in situ |
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