Comprehensive Analyses of White-Handed Gibbon Chromosomes Enables Access to 92 Evolutionary Conserved Breakpoints Compared to the Human Genome

Gibbon species (Hylobatidae) impress with an unusually high number of numerical and structural chromosomal changes within the family itself as well as compared to other Hominoidea including humans. In former studies applying molecular cytogenetic methods, 86 evolutionary conserved breakpoints (ECBs)...

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Veröffentlicht in:Cytogenetic and genome research 2015-01, Vol.145 (1), p.42-49
Hauptverfasser: Weise, Anja, Kosyakova, Nadezda, Voigt, Martin, Aust, Nadine, Mrasek, Kristin, Löhmer, Sharon, Rubtsov, Nikolai, Karamysheva, Tatyana V., Trifonov, Vladimir A., Hardekopf, David, Jančušková, Tereza, Pekova, Sona, Wilhelm, Kathleen, Liehr, Thomas, Fan, Xiaobo
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container_issue 1
container_start_page 42
container_title Cytogenetic and genome research
container_volume 145
creator Weise, Anja
Kosyakova, Nadezda
Voigt, Martin
Aust, Nadine
Mrasek, Kristin
Löhmer, Sharon
Rubtsov, Nikolai
Karamysheva, Tatyana V.
Trifonov, Vladimir A.
Hardekopf, David
Jančušková, Tereza
Pekova, Sona
Wilhelm, Kathleen
Liehr, Thomas
Fan, Xiaobo
description Gibbon species (Hylobatidae) impress with an unusually high number of numerical and structural chromosomal changes within the family itself as well as compared to other Hominoidea including humans. In former studies applying molecular cytogenetic methods, 86 evolutionary conserved breakpoints (ECBs) were reported in the white-handed gibbon (Hylobates lar, HLA) with respect to the human genome. To analyze those ECBs in more detail and also to achieve a better understanding of the fast karyotype evolution in Hylobatidae, molecular data for these regions are indispensably necessary. In the present study, we obtained whole chromosome-specific probes by microdissection of all 21 HLA autosomes and prepared them for aCGH. Locus-specific DNA probes were also used for further molecular cytogenetic characterization of selected regions. Thus, we could map 6 yet unreported ECBs in HLA with respect to the human genome. Additionally, in 26 of the 86 previously reported ECBs, the present approach enabled a more precise breakpoint mapping. Interestingly, a preferred localization of ECBs within segmental duplications, copy number variant regions, and fragile sites was observed.
doi_str_mv 10.1159/000381764
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subjects Animals
Cell Line
Chromosome Breakpoints
Chromosome Mapping
Chromosomes, Mammalian - genetics
Comparative Genomic Hybridization
Conserved Sequence
Evolution, Molecular
Female
Genome, Human - genetics
Humans
Hylobates
Hylobates lar
Hylobatidae
Karyotype
Original Article
Species Specificity
title Comprehensive Analyses of White-Handed Gibbon Chromosomes Enables Access to 92 Evolutionary Conserved Breakpoints Compared to the Human Genome
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