Comprehensive Analyses of White-Handed Gibbon Chromosomes Enables Access to 92 Evolutionary Conserved Breakpoints Compared to the Human Genome
Gibbon species (Hylobatidae) impress with an unusually high number of numerical and structural chromosomal changes within the family itself as well as compared to other Hominoidea including humans. In former studies applying molecular cytogenetic methods, 86 evolutionary conserved breakpoints (ECBs)...
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creator | Weise, Anja Kosyakova, Nadezda Voigt, Martin Aust, Nadine Mrasek, Kristin Löhmer, Sharon Rubtsov, Nikolai Karamysheva, Tatyana V. Trifonov, Vladimir A. Hardekopf, David Jančušková, Tereza Pekova, Sona Wilhelm, Kathleen Liehr, Thomas Fan, Xiaobo |
description | Gibbon species (Hylobatidae) impress with an unusually high number of numerical and structural chromosomal changes within the family itself as well as compared to other Hominoidea including humans. In former studies applying molecular cytogenetic methods, 86 evolutionary conserved breakpoints (ECBs) were reported in the white-handed gibbon (Hylobates lar, HLA) with respect to the human genome. To analyze those ECBs in more detail and also to achieve a better understanding of the fast karyotype evolution in Hylobatidae, molecular data for these regions are indispensably necessary. In the present study, we obtained whole chromosome-specific probes by microdissection of all 21 HLA autosomes and prepared them for aCGH. Locus-specific DNA probes were also used for further molecular cytogenetic characterization of selected regions. Thus, we could map 6 yet unreported ECBs in HLA with respect to the human genome. Additionally, in 26 of the 86 previously reported ECBs, the present approach enabled a more precise breakpoint mapping. Interestingly, a preferred localization of ECBs within segmental duplications, copy number variant regions, and fragile sites was observed. |
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In former studies applying molecular cytogenetic methods, 86 evolutionary conserved breakpoints (ECBs) were reported in the white-handed gibbon (Hylobates lar, HLA) with respect to the human genome. To analyze those ECBs in more detail and also to achieve a better understanding of the fast karyotype evolution in Hylobatidae, molecular data for these regions are indispensably necessary. In the present study, we obtained whole chromosome-specific probes by microdissection of all 21 HLA autosomes and prepared them for aCGH. Locus-specific DNA probes were also used for further molecular cytogenetic characterization of selected regions. Thus, we could map 6 yet unreported ECBs in HLA with respect to the human genome. Additionally, in 26 of the 86 previously reported ECBs, the present approach enabled a more precise breakpoint mapping. Interestingly, a preferred localization of ECBs within segmental duplications, copy number variant regions, and fragile sites was observed.</description><identifier>ISSN: 1424-8581</identifier><identifier>EISSN: 1424-859X</identifier><identifier>DOI: 10.1159/000381764</identifier><identifier>PMID: 25926034</identifier><language>eng</language><publisher>Basel, Switzerland: S. Karger AG</publisher><subject>Animals ; Cell Line ; Chromosome Breakpoints ; Chromosome Mapping ; Chromosomes, Mammalian - genetics ; Comparative Genomic Hybridization ; Conserved Sequence ; Evolution, Molecular ; Female ; Genome, Human - genetics ; Humans ; Hylobates ; Hylobates lar ; Hylobatidae ; Karyotype ; Original Article ; Species Specificity</subject><ispartof>Cytogenetic and genome research, 2015-01, Vol.145 (1), p.42-49</ispartof><rights>2015 S. Karger AG, Basel</rights><rights>2015 S. Karger AG, Basel.</rights><rights>Copyright (c) 2015 S. Karger AG, Basel</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c472t-f9d9223e9eb4a86006d6aa9db21ec74552673a16cd0310d0777618e9c54495cb3</citedby><cites>FETCH-LOGICAL-c472t-f9d9223e9eb4a86006d6aa9db21ec74552673a16cd0310d0777618e9c54495cb3</cites><orcidid>0000-0003-0454-8359</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,776,780,2422,27903,27904</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/25926034$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Weise, Anja</creatorcontrib><creatorcontrib>Kosyakova, Nadezda</creatorcontrib><creatorcontrib>Voigt, Martin</creatorcontrib><creatorcontrib>Aust, Nadine</creatorcontrib><creatorcontrib>Mrasek, Kristin</creatorcontrib><creatorcontrib>Löhmer, Sharon</creatorcontrib><creatorcontrib>Rubtsov, Nikolai</creatorcontrib><creatorcontrib>Karamysheva, Tatyana V.</creatorcontrib><creatorcontrib>Trifonov, Vladimir A.</creatorcontrib><creatorcontrib>Hardekopf, David</creatorcontrib><creatorcontrib>Jančušková, Tereza</creatorcontrib><creatorcontrib>Pekova, Sona</creatorcontrib><creatorcontrib>Wilhelm, Kathleen</creatorcontrib><creatorcontrib>Liehr, Thomas</creatorcontrib><creatorcontrib>Fan, Xiaobo</creatorcontrib><title>Comprehensive Analyses of White-Handed Gibbon Chromosomes Enables Access to 92 Evolutionary Conserved Breakpoints Compared to the Human Genome</title><title>Cytogenetic and genome research</title><addtitle>Cytogenet Genome Res</addtitle><description>Gibbon species (Hylobatidae) impress with an unusually high number of numerical and structural chromosomal changes within the family itself as well as compared to other Hominoidea including humans. In former studies applying molecular cytogenetic methods, 86 evolutionary conserved breakpoints (ECBs) were reported in the white-handed gibbon (Hylobates lar, HLA) with respect to the human genome. To analyze those ECBs in more detail and also to achieve a better understanding of the fast karyotype evolution in Hylobatidae, molecular data for these regions are indispensably necessary. In the present study, we obtained whole chromosome-specific probes by microdissection of all 21 HLA autosomes and prepared them for aCGH. Locus-specific DNA probes were also used for further molecular cytogenetic characterization of selected regions. Thus, we could map 6 yet unreported ECBs in HLA with respect to the human genome. Additionally, in 26 of the 86 previously reported ECBs, the present approach enabled a more precise breakpoint mapping. Interestingly, a preferred localization of ECBs within segmental duplications, copy number variant regions, and fragile sites was observed.</description><subject>Animals</subject><subject>Cell Line</subject><subject>Chromosome Breakpoints</subject><subject>Chromosome Mapping</subject><subject>Chromosomes, Mammalian - genetics</subject><subject>Comparative Genomic Hybridization</subject><subject>Conserved Sequence</subject><subject>Evolution, Molecular</subject><subject>Female</subject><subject>Genome, Human - genetics</subject><subject>Humans</subject><subject>Hylobates</subject><subject>Hylobates lar</subject><subject>Hylobatidae</subject><subject>Karyotype</subject><subject>Original Article</subject><subject>Species Specificity</subject><issn>1424-8581</issn><issn>1424-859X</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2015</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><sourceid>GNUQQ</sourceid><recordid>eNqF0T1v2zAQBmAiaJGkaYfsRUGgSzOoJSl-jo7g2AUCZGnRbgJFnWMlEumSkoH8ifzmMrHjIUumOxDPewTuEDqn5DulwvwghJSaKsmP0CnljBdamL_vDr2mJ-hDSneEUM2FPEYnTBgmSclP0WMVhk2ENfjUbQHPvO0fEiQcVvjPuhuhWFrfQosXXdMEj6t1DENIYchk7m3T5zpzDlLCY8CG4fk29NPYBW_jA66CTxC3OX4Zwd5vQufHhJ9-tDE_5sS4BrycBuvxAnye-hG9X9k-wad9PUO_r-a_qmVxfbP4Wc2uC8cVG4uVaQ1jJRhouNWSENlKa03bMApOcSGYVKWl0rWkpKQlSilJNRgnODfCNeUZ-rabu4nh3wRprIcuOeh76yFMqaY5IIVS1LxNpSGMktLoTL--ondhinmlz8owzrUmWV3slIshpQirehO7Ie-rpqR-umd9uGe2X_YTp2aA9iBfDpjB5x24t_EW4gHs8_8B6bSi8w</recordid><startdate>20150101</startdate><enddate>20150101</enddate><creator>Weise, Anja</creator><creator>Kosyakova, Nadezda</creator><creator>Voigt, Martin</creator><creator>Aust, Nadine</creator><creator>Mrasek, Kristin</creator><creator>Löhmer, Sharon</creator><creator>Rubtsov, Nikolai</creator><creator>Karamysheva, Tatyana V.</creator><creator>Trifonov, Vladimir A.</creator><creator>Hardekopf, David</creator><creator>Jančušková, Tereza</creator><creator>Pekova, Sona</creator><creator>Wilhelm, Kathleen</creator><creator>Liehr, Thomas</creator><creator>Fan, Xiaobo</creator><general>S. 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In former studies applying molecular cytogenetic methods, 86 evolutionary conserved breakpoints (ECBs) were reported in the white-handed gibbon (Hylobates lar, HLA) with respect to the human genome. To analyze those ECBs in more detail and also to achieve a better understanding of the fast karyotype evolution in Hylobatidae, molecular data for these regions are indispensably necessary. In the present study, we obtained whole chromosome-specific probes by microdissection of all 21 HLA autosomes and prepared them for aCGH. Locus-specific DNA probes were also used for further molecular cytogenetic characterization of selected regions. Thus, we could map 6 yet unreported ECBs in HLA with respect to the human genome. Additionally, in 26 of the 86 previously reported ECBs, the present approach enabled a more precise breakpoint mapping. 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subjects | Animals Cell Line Chromosome Breakpoints Chromosome Mapping Chromosomes, Mammalian - genetics Comparative Genomic Hybridization Conserved Sequence Evolution, Molecular Female Genome, Human - genetics Humans Hylobates Hylobates lar Hylobatidae Karyotype Original Article Species Specificity |
title | Comprehensive Analyses of White-Handed Gibbon Chromosomes Enables Access to 92 Evolutionary Conserved Breakpoints Compared to the Human Genome |
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