Expression and Functional Analysis of Dkk1 during Early Gonadal Development
WNT signalling plays a central role in mammalian sex determination by promoting ovarian development and repressing aspects of testis development in the early gonad. Dickkopf homolog 1 (DKK1) is a WNT signalling antagonist that plays critical roles in multiple developmental systems by modulating WNT...
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| Veröffentlicht in: | Sexual development 2011-01, Vol.5 (3), p.124-130 |
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| container_title | Sexual development |
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| creator | Combes, A.N. Bowles, J. Feng, C.-W. Chiu, H.S. Khoo, P.-L. Jackson, A. Little, M.H. Tam, P.P.L. Koopman, P. |
| description | WNT signalling plays a central role in mammalian sex determination by promoting ovarian development and repressing aspects of testis development in the early gonad. Dickkopf homolog 1 (DKK1) is a WNT signalling antagonist that plays critical roles in multiple developmental systems by modulating WNT activity. Here, we examined the role of DKK1 in mouse sex determination and early gonadal development. Dkk1 mRNA was upregulated sex-specifically during testis differentiation, suggesting that DKK1 could repress WNT signalling in the developing testis. However, we observed overtly normal testis development in Dkk1-null XY gonads, and found no significant upregulation of Axin2 or Sp5 that would indicate increased canonical WNT signalling. Nor did we find significant differences in expression of key markers of testis and ovarian development. We propose that DKK1 may play a protective role that is not unmasked by loss-of-function in the absence of other stressors. |
| doi_str_mv | 10.1159/000327709 |
| format | Article |
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Dickkopf homolog 1 (DKK1) is a WNT signalling antagonist that plays critical roles in multiple developmental systems by modulating WNT activity. Here, we examined the role of DKK1 in mouse sex determination and early gonadal development. Dkk1 mRNA was upregulated sex-specifically during testis differentiation, suggesting that DKK1 could repress WNT signalling in the developing testis. However, we observed overtly normal testis development in Dkk1-null XY gonads, and found no significant upregulation of Axin2 or Sp5 that would indicate increased canonical WNT signalling. Nor did we find significant differences in expression of key markers of testis and ovarian development. We propose that DKK1 may play a protective role that is not unmasked by loss-of-function in the absence of other stressors.</description><identifier>ISSN: 1661-5425</identifier><identifier>EISSN: 1661-5433</identifier><identifier>DOI: 10.1159/000327709</identifier><identifier>PMID: 21654186</identifier><language>eng</language><publisher>Basel, Switzerland: S. Karger AG</publisher><subject>Animals ; Female ; Gonads - growth & development ; Gonads - metabolism ; In Situ Hybridization ; Intercellular Signaling Peptides and Proteins - genetics ; Intercellular Signaling Peptides and Proteins - metabolism ; Male ; Mice ; Mice, Knockout ; Original ; Original Article ; Ovary - growth & development ; Ovary - metabolism ; Reverse Transcriptase Polymerase Chain Reaction ; Sex Determination Processes - genetics ; Sex Determination Processes - physiology ; Testis - growth & development ; Testis - metabolism</subject><ispartof>Sexual development, 2011-01, Vol.5 (3), p.124-130</ispartof><rights>2011 S. Karger AG, Basel</rights><rights>Copyright © 2011 S. Karger AG, Basel.</rights><rights>Copyright © 2011 by S. Karger AG, Basel 2011</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c395t-5e9d4183da9c07c861f57896763a01622fb05456a0dc9fbda91dca81abdbe3873</citedby><cites>FETCH-LOGICAL-c395t-5e9d4183da9c07c861f57896763a01622fb05456a0dc9fbda91dca81abdbe3873</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>230,314,776,780,881,2423,27901,27902</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/21654186$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Combes, A.N.</creatorcontrib><creatorcontrib>Bowles, J.</creatorcontrib><creatorcontrib>Feng, C.-W.</creatorcontrib><creatorcontrib>Chiu, H.S.</creatorcontrib><creatorcontrib>Khoo, P.-L.</creatorcontrib><creatorcontrib>Jackson, A.</creatorcontrib><creatorcontrib>Little, M.H.</creatorcontrib><creatorcontrib>Tam, P.P.L.</creatorcontrib><creatorcontrib>Koopman, P.</creatorcontrib><title>Expression and Functional Analysis of Dkk1 during Early Gonadal Development</title><title>Sexual development</title><addtitle>Sex Dev</addtitle><description>WNT signalling plays a central role in mammalian sex determination by promoting ovarian development and repressing aspects of testis development in the early gonad. Dickkopf homolog 1 (DKK1) is a WNT signalling antagonist that plays critical roles in multiple developmental systems by modulating WNT activity. Here, we examined the role of DKK1 in mouse sex determination and early gonadal development. Dkk1 mRNA was upregulated sex-specifically during testis differentiation, suggesting that DKK1 could repress WNT signalling in the developing testis. However, we observed overtly normal testis development in Dkk1-null XY gonads, and found no significant upregulation of Axin2 or Sp5 that would indicate increased canonical WNT signalling. Nor did we find significant differences in expression of key markers of testis and ovarian development. 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Dickkopf homolog 1 (DKK1) is a WNT signalling antagonist that plays critical roles in multiple developmental systems by modulating WNT activity. Here, we examined the role of DKK1 in mouse sex determination and early gonadal development. Dkk1 mRNA was upregulated sex-specifically during testis differentiation, suggesting that DKK1 could repress WNT signalling in the developing testis. However, we observed overtly normal testis development in Dkk1-null XY gonads, and found no significant upregulation of Axin2 or Sp5 that would indicate increased canonical WNT signalling. Nor did we find significant differences in expression of key markers of testis and ovarian development. We propose that DKK1 may play a protective role that is not unmasked by loss-of-function in the absence of other stressors.</abstract><cop>Basel, Switzerland</cop><pub>S. Karger AG</pub><pmid>21654186</pmid><doi>10.1159/000327709</doi><tpages>7</tpages><oa>free_for_read</oa></addata></record> |
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| source | Karger Journals; MEDLINE; Alma/SFX Local Collection |
| subjects | Animals Female Gonads - growth & development Gonads - metabolism In Situ Hybridization Intercellular Signaling Peptides and Proteins - genetics Intercellular Signaling Peptides and Proteins - metabolism Male Mice Mice, Knockout Original Original Article Ovary - growth & development Ovary - metabolism Reverse Transcriptase Polymerase Chain Reaction Sex Determination Processes - genetics Sex Determination Processes - physiology Testis - growth & development Testis - metabolism |
| title | Expression and Functional Analysis of Dkk1 during Early Gonadal Development |
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