Expression of Splice Variants of the Human ADAM15 Gene and Strong Interaction between the Cytoplasmic Domain of One Variant and Src Family Proteins Lck and Hck
Objective: The aim of the present study was to show variant species of ADAM15 and unique Src homology 3 (SH3)-binding motifs, which strongly bound Src family proteins compared with ADAM15. Methods and Results: RT-PCR using primers for the cytoplasmic domain revealed the presence of different species...
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| Veröffentlicht in: | Pathobiology (Basel) 2004-01, Vol.71 (4), p.185-192 |
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| creator | Yasui, Atsushi Matsuura, Keiko Shimizu, Eiichi Hijiya, Naoki Higuchi, Yasunori Yamamoto, Shunsuke |
| description | Objective: The aim of the present study was to show variant species of ADAM15 and unique Src homology 3 (SH3)-binding motifs, which strongly bound Src family proteins compared with ADAM15. Methods and Results: RT-PCR using primers for the cytoplasmic domain revealed the presence of different species, designated ADAM15v1 and ADAM15v2, which had characteristic SH3-binding class I and class II motifs. The mRNA of ADAM15v1 and ADAM15v2 was mainly found in peripheral blood mononuclear cells, T lymphocytes and monocytic cell lines. ADAM15v2 protein interacted more strongly with the Src family proteins Lck and Hck than did ADAM15 protein, as examined by pull-down analysis and immunoprecipitation followed by immunoblot analysis. The binding with Lck and Hck was enhanced by the phosphorylation of ADAM15v2 protein. Conclusions: These results suggest that the cytoplasmic domain of ADAM15v2 strongly interacts with Lck and Hck and regulates leukocyte function. |
| doi_str_mv | 10.1159/000078672 |
| format | Article |
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Methods and Results: RT-PCR using primers for the cytoplasmic domain revealed the presence of different species, designated ADAM15v1 and ADAM15v2, which had characteristic SH3-binding class I and class II motifs. The mRNA of ADAM15v1 and ADAM15v2 was mainly found in peripheral blood mononuclear cells, T lymphocytes and monocytic cell lines. ADAM15v2 protein interacted more strongly with the Src family proteins Lck and Hck than did ADAM15 protein, as examined by pull-down analysis and immunoprecipitation followed by immunoblot analysis. The binding with Lck and Hck was enhanced by the phosphorylation of ADAM15v2 protein. Conclusions: These results suggest that the cytoplasmic domain of ADAM15v2 strongly interacts with Lck and Hck and regulates leukocyte function.</description><identifier>ISSN: 1015-2008</identifier><identifier>EISSN: 1423-0291</identifier><identifier>DOI: 10.1159/000078672</identifier><identifier>PMID: 15263807</identifier><identifier>CODEN: PATHEF</identifier><language>eng</language><publisher>Basel, Switzerland: S. Karger AG</publisher><subject>ADAM Proteins ; Alternative Splicing ; Amino Acid Sequence ; Base Sequence ; Cell Line ; DNA, Recombinant ; Humans ; Leukocytes, Mononuclear - metabolism ; Lymphocyte Specific Protein Tyrosine Kinase p56(lck) - genetics ; Lymphocyte Specific Protein Tyrosine Kinase p56(lck) - metabolism ; Lymphocytes ; Membrane Proteins - classification ; Membrane Proteins - genetics ; Metalloendopeptidases - classification ; Metalloendopeptidases - genetics ; Molecular Sequence Data ; Original Paper ; Protein Structure, Tertiary - genetics ; Protein-Tyrosine Kinases - genetics ; Protein-Tyrosine Kinases - metabolism ; Proto-Oncogene Proteins - genetics ; Proto-Oncogene Proteins - metabolism ; Proto-Oncogene Proteins c-hck ; Reverse Transcriptase Polymerase Chain Reaction ; RNA, Messenger - analysis</subject><ispartof>Pathobiology (Basel), 2004-01, Vol.71 (4), p.185-192</ispartof><rights>2004 S. Karger AG, Basel</rights><rights>Copyright 2004 S. Karger AG, Basel</rights><rights>Copyright (c) 2004 S. Karger AG, Basel</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c452t-1cd872432c62e932811a4398c5e60d99b2e667df75f328ca07c012cdc2e18bfd3</citedby><cites>FETCH-LOGICAL-c452t-1cd872432c62e932811a4398c5e60d99b2e667df75f328ca07c012cdc2e18bfd3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>315,781,785,2430,27928,27929</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/15263807$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Yasui, Atsushi</creatorcontrib><creatorcontrib>Matsuura, Keiko</creatorcontrib><creatorcontrib>Shimizu, Eiichi</creatorcontrib><creatorcontrib>Hijiya, Naoki</creatorcontrib><creatorcontrib>Higuchi, Yasunori</creatorcontrib><creatorcontrib>Yamamoto, Shunsuke</creatorcontrib><title>Expression of Splice Variants of the Human ADAM15 Gene and Strong Interaction between the Cytoplasmic Domain of One Variant and Src Family Proteins Lck and Hck</title><title>Pathobiology (Basel)</title><addtitle>Pathobiology</addtitle><description>Objective: The aim of the present study was to show variant species of ADAM15 and unique Src homology 3 (SH3)-binding motifs, which strongly bound Src family proteins compared with ADAM15. 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Conclusions: These results suggest that the cytoplasmic domain of ADAM15v2 strongly interacts with Lck and Hck and regulates leukocyte function.</description><subject>ADAM Proteins</subject><subject>Alternative Splicing</subject><subject>Amino Acid Sequence</subject><subject>Base Sequence</subject><subject>Cell Line</subject><subject>DNA, Recombinant</subject><subject>Humans</subject><subject>Leukocytes, Mononuclear - metabolism</subject><subject>Lymphocyte Specific Protein Tyrosine Kinase p56(lck) - genetics</subject><subject>Lymphocyte Specific Protein Tyrosine Kinase p56(lck) - metabolism</subject><subject>Lymphocytes</subject><subject>Membrane Proteins - classification</subject><subject>Membrane Proteins - genetics</subject><subject>Metalloendopeptidases - classification</subject><subject>Metalloendopeptidases - genetics</subject><subject>Molecular Sequence Data</subject><subject>Original Paper</subject><subject>Protein Structure, Tertiary - genetics</subject><subject>Protein-Tyrosine Kinases - genetics</subject><subject>Protein-Tyrosine Kinases - metabolism</subject><subject>Proto-Oncogene Proteins - genetics</subject><subject>Proto-Oncogene Proteins - metabolism</subject><subject>Proto-Oncogene Proteins c-hck</subject><subject>Reverse Transcriptase Polymerase Chain Reaction</subject><subject>RNA, Messenger - analysis</subject><issn>1015-2008</issn><issn>1423-0291</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2004</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><sourceid>GNUQQ</sourceid><recordid>eNqFkUtr3DAUhUVpaB7toutCEVkEunArXduyvBwmjwlMSSBpt0YjX6fO2JIryTTza_JXq3lkCqVQbSR0vnMu0iHkPWefOc_LLyyuQooCXpEjnkGaMCj563hmPE-AMXlIjr1_jJRkgr0hhzwHkUpWHJHni6fBofetNdQ29G7oWo30u3KtMsGvr8IPpLOxV4ZOzidfeU6v0CBVpqZ3wVnzQK9NQKd0WEcsMPxCNBvTdBXs0Cnft5qe2161mwk3Zh-_DXGaXqq-7Vb01tmArfF0rpcbbaaXb8lBozqP73b7Cfl2eXE_nSXzm6vr6WSe6CyHkHBdywKyFLQALFOQnKssLaXOUbC6LBeAQhR1U-RNFLVihWYcdK0BuVw0dXpCzra5g7M_R_Sh6luvseuUQTv6KrqBx1_-LwgcGHAhI3j6F_hoR2fiIyqATKQZl2vo0xbSznrvsKkG1_bKrSrOqnW31b7byH7cBY6LHus_5K7MCHzYAkvlHtDtgRf76T_V28n9BqiGukl_AzK4sbE</recordid><startdate>20040101</startdate><enddate>20040101</enddate><creator>Yasui, Atsushi</creator><creator>Matsuura, Keiko</creator><creator>Shimizu, Eiichi</creator><creator>Hijiya, Naoki</creator><creator>Higuchi, Yasunori</creator><creator>Yamamoto, Shunsuke</creator><general>S. 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metabolism</topic><topic>Lymphocyte Specific Protein Tyrosine Kinase p56(lck) - genetics</topic><topic>Lymphocyte Specific Protein Tyrosine Kinase p56(lck) - metabolism</topic><topic>Lymphocytes</topic><topic>Membrane Proteins - classification</topic><topic>Membrane Proteins - genetics</topic><topic>Metalloendopeptidases - classification</topic><topic>Metalloendopeptidases - genetics</topic><topic>Molecular Sequence Data</topic><topic>Original Paper</topic><topic>Protein Structure, Tertiary - genetics</topic><topic>Protein-Tyrosine Kinases - genetics</topic><topic>Protein-Tyrosine Kinases - metabolism</topic><topic>Proto-Oncogene Proteins - genetics</topic><topic>Proto-Oncogene Proteins - metabolism</topic><topic>Proto-Oncogene Proteins c-hck</topic><topic>Reverse Transcriptase Polymerase Chain Reaction</topic><topic>RNA, Messenger - analysis</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Yasui, Atsushi</creatorcontrib><creatorcontrib>Matsuura, Keiko</creatorcontrib><creatorcontrib>Shimizu, Eiichi</creatorcontrib><creatorcontrib>Hijiya, Naoki</creatorcontrib><creatorcontrib>Higuchi, Yasunori</creatorcontrib><creatorcontrib>Yamamoto, Shunsuke</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Nursing & Allied Health Database</collection><collection>Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Biology Database (Alumni Edition)</collection><collection>Medical Database (Alumni Edition)</collection><collection>Science Database (Alumni Edition)</collection><collection>STEM Database</collection><collection>ProQuest Pharma Collection</collection><collection>Public Health Database</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest Central UK/Ireland</collection><collection>ProQuest Central Essentials</collection><collection>Biological Science Collection</collection><collection>Proquest Central</collection><collection>Natural Science Collection</collection><collection>Earth, Atmospheric & Aquatic Science Collection</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central Korea</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Central Student</collection><collection>SciTech Premium Collection</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>Nursing & Allied Health Database (Alumni Edition)</collection><collection>ProQuest Biological Science Collection</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Medical Database</collection><collection>Science Database</collection><collection>Biological Science Database</collection><collection>Nursing & Allied Health Premium</collection><collection>Earth, Atmospheric & Aquatic Science Database</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central Basic</collection><collection>SIRS Editorial</collection><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Genetics Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Pathobiology (Basel)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Yasui, Atsushi</au><au>Matsuura, Keiko</au><au>Shimizu, Eiichi</au><au>Hijiya, Naoki</au><au>Higuchi, Yasunori</au><au>Yamamoto, Shunsuke</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Expression of Splice Variants of the Human ADAM15 Gene and Strong Interaction between the Cytoplasmic Domain of One Variant and Src Family Proteins Lck and Hck</atitle><jtitle>Pathobiology (Basel)</jtitle><addtitle>Pathobiology</addtitle><date>2004-01-01</date><risdate>2004</risdate><volume>71</volume><issue>4</issue><spage>185</spage><epage>192</epage><pages>185-192</pages><issn>1015-2008</issn><eissn>1423-0291</eissn><coden>PATHEF</coden><abstract>Objective: The aim of the present study was to show variant species of ADAM15 and unique Src homology 3 (SH3)-binding motifs, which strongly bound Src family proteins compared with ADAM15. Methods and Results: RT-PCR using primers for the cytoplasmic domain revealed the presence of different species, designated ADAM15v1 and ADAM15v2, which had characteristic SH3-binding class I and class II motifs. The mRNA of ADAM15v1 and ADAM15v2 was mainly found in peripheral blood mononuclear cells, T lymphocytes and monocytic cell lines. ADAM15v2 protein interacted more strongly with the Src family proteins Lck and Hck than did ADAM15 protein, as examined by pull-down analysis and immunoprecipitation followed by immunoblot analysis. The binding with Lck and Hck was enhanced by the phosphorylation of ADAM15v2 protein. Conclusions: These results suggest that the cytoplasmic domain of ADAM15v2 strongly interacts with Lck and Hck and regulates leukocyte function.</abstract><cop>Basel, Switzerland</cop><pub>S. Karger AG</pub><pmid>15263807</pmid><doi>10.1159/000078672</doi><tpages>8</tpages></addata></record> |
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| source | MEDLINE; Karger Journals |
| subjects | ADAM Proteins Alternative Splicing Amino Acid Sequence Base Sequence Cell Line DNA, Recombinant Humans Leukocytes, Mononuclear - metabolism Lymphocyte Specific Protein Tyrosine Kinase p56(lck) - genetics Lymphocyte Specific Protein Tyrosine Kinase p56(lck) - metabolism Lymphocytes Membrane Proteins - classification Membrane Proteins - genetics Metalloendopeptidases - classification Metalloendopeptidases - genetics Molecular Sequence Data Original Paper Protein Structure, Tertiary - genetics Protein-Tyrosine Kinases - genetics Protein-Tyrosine Kinases - metabolism Proto-Oncogene Proteins - genetics Proto-Oncogene Proteins - metabolism Proto-Oncogene Proteins c-hck Reverse Transcriptase Polymerase Chain Reaction RNA, Messenger - analysis |
| title | Expression of Splice Variants of the Human ADAM15 Gene and Strong Interaction between the Cytoplasmic Domain of One Variant and Src Family Proteins Lck and Hck |
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