Active Relaxation of Human Gallbladder Muscle Is Mediated by ATP-Sensitive Potassium Channels
Background: Active and significant relaxation of the human gallbladder must be one of the facets of its motility during both the filling and emptying cycle. Conflicting reports about the presence or significance of nitric oxide have been reported in the literature. The aim of this study was to inves...
Gespeichert in:
| Veröffentlicht in: | Digestion 2002-01, Vol.65 (4), p.220-226 |
|---|---|
| Hauptverfasser: | , , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 226 |
|---|---|
| container_issue | 4 |
| container_start_page | 220 |
| container_title | Digestion |
| container_volume | 65 |
| creator | Bird, N.C. Ahmed, R. Chess-Williams, R. Johnson, A.G. |
| description | Background: Active and significant relaxation of the human gallbladder must be one of the facets of its motility during both the filling and emptying cycle. Conflicting reports about the presence or significance of nitric oxide have been reported in the literature. The aim of this study was to investigate the role of nitric oxide and K ATP channels in human gallbladder muscle using isolated strips from human gallbladder. Methods: Full thickness strips were obtained from 56 human gallbladders and suspended under isometric tension in organ baths. The effect of nitric oxide donors and inhibitors on cholecystokinin octapeptide- and carbachol-induced contraction was examined. In separate experiments the effect of the K ATP channel activator, cromakalim, and the inhibitor, glibenclamide, were determined. Results: Cromakalim induced a significant relaxation of agonist-induced contraction in human gallbladder in vitro, an effect which was abolished by the K ATP channel inhibitor glibenclamide. No evidence of significant nitric oxide involvement in relaxation was observed. Conclusions: This study has demonstrated the presence of K ATP channels in human gallbladder for the first time. These are capable of causing significant relaxation in the presence of hormonal and muscarinic agonists and may represent a major pathway for gallbladder relaxation. |
| doi_str_mv | 10.1159/000063815 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_crossref_primary_10_1159_000063815</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>264256711</sourcerecordid><originalsourceid>FETCH-LOGICAL-c384t-311398c953996b86504358e3c9f4cd67c39ef31486cdb08160a156ef14e0863e3</originalsourceid><addsrcrecordid>eNpt0M9L5DAUB_AgKzqrHjwLSxQUPFSTvDaTHIdZHQcUxR9HKWn66tZN2zFpl53_3jozKoi5hJAP3_f4ErLL2QnniT5l_ZGgeLJGBjwWPNJKDn-QAWNcREIJ2CQ_Q3h-e-oYNsgmFwJ0LGFAHke2Lf8hvUVn_pu2bGraFPSiq0xNJ8a5zJk8R0-vumAd0mmgV5iXpsWcZnM6ur-J7rAO5SLjpmlNCGVX0fEfU9fowjZZL4wLuLO6t8jD-dn9-CK6vJ5Mx6PLyIKK2wg4B62sTkBrmSmZsBgShWB1EdtcDi1oLIDHSto8Y4pLZngiseAxMiUBYYscLXNnvnnpMLRpVQaLzpkamy6kQ8GZGILq4f4X-Nx0vu53S5Xuq2MLc7w01jcheCzSmS8r4-cpZ-lb3-lH3739tcrrsgrzT7kquAeHK2CCNa7wprZl-HSgRSK17t3e0v01_gn9B3gfc_Dt7-_pZAHSWV7AK0y7mUM</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>89142038</pqid></control><display><type>article</type><title>Active Relaxation of Human Gallbladder Muscle Is Mediated by ATP-Sensitive Potassium Channels</title><source>MEDLINE</source><source>Karger Journals Complete</source><creator>Bird, N.C. ; Ahmed, R. ; Chess-Williams, R. ; Johnson, A.G.</creator><creatorcontrib>Bird, N.C. ; Ahmed, R. ; Chess-Williams, R. ; Johnson, A.G.</creatorcontrib><description>Background: Active and significant relaxation of the human gallbladder must be one of the facets of its motility during both the filling and emptying cycle. Conflicting reports about the presence or significance of nitric oxide have been reported in the literature. The aim of this study was to investigate the role of nitric oxide and K ATP channels in human gallbladder muscle using isolated strips from human gallbladder. Methods: Full thickness strips were obtained from 56 human gallbladders and suspended under isometric tension in organ baths. The effect of nitric oxide donors and inhibitors on cholecystokinin octapeptide- and carbachol-induced contraction was examined. In separate experiments the effect of the K ATP channel activator, cromakalim, and the inhibitor, glibenclamide, were determined. Results: Cromakalim induced a significant relaxation of agonist-induced contraction in human gallbladder in vitro, an effect which was abolished by the K ATP channel inhibitor glibenclamide. No evidence of significant nitric oxide involvement in relaxation was observed. Conclusions: This study has demonstrated the presence of K ATP channels in human gallbladder for the first time. These are capable of causing significant relaxation in the presence of hormonal and muscarinic agonists and may represent a major pathway for gallbladder relaxation.</description><identifier>ISSN: 0012-2823</identifier><identifier>EISSN: 1421-9867</identifier><identifier>DOI: 10.1159/000063815</identifier><identifier>PMID: 12239463</identifier><identifier>CODEN: DIGEBW</identifier><language>eng</language><publisher>Basel, Switzerland: Karger</publisher><subject>Adenosine Triphosphate - physiology ; Animals ; Biological and medical sciences ; Carbachol - pharmacology ; Cromakalim - pharmacology ; Digestive system ; Gallbladder - physiology ; Glyburide - pharmacology ; Guinea Pigs ; Humans ; In Vitro Techniques ; Investigative techniques, diagnostic techniques (general aspects) ; Medical sciences ; Muscle Relaxation - drug effects ; Muscle Relaxation - physiology ; Muscle, Smooth - physiology ; Nitric Oxide - agonists ; Nitric Oxide - antagonists & inhibitors ; Nitric Oxide - physiology ; Original Paper: Motility ; Pathology. Cytology. Biochemistry. Spectrometry. Miscellaneous investigative techniques ; Potassium Channels - chemistry ; Potassium Channels - drug effects ; Potassium Channels - physiology ; Sincalide - pharmacology</subject><ispartof>Digestion, 2002-01, Vol.65 (4), p.220-226</ispartof><rights>2002 S. Karger AG, Basel</rights><rights>2002 INIST-CNRS</rights><rights>Copyright 2002 S. Karger AG, Basel</rights><rights>Copyright S. Karger AG 2002</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c384t-311398c953996b86504358e3c9f4cd67c39ef31486cdb08160a156ef14e0863e3</citedby><cites>FETCH-LOGICAL-c384t-311398c953996b86504358e3c9f4cd67c39ef31486cdb08160a156ef14e0863e3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,2429,27924,27925</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=13925699$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/12239463$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Bird, N.C.</creatorcontrib><creatorcontrib>Ahmed, R.</creatorcontrib><creatorcontrib>Chess-Williams, R.</creatorcontrib><creatorcontrib>Johnson, A.G.</creatorcontrib><title>Active Relaxation of Human Gallbladder Muscle Is Mediated by ATP-Sensitive Potassium Channels</title><title>Digestion</title><addtitle>Digestion</addtitle><description>Background: Active and significant relaxation of the human gallbladder must be one of the facets of its motility during both the filling and emptying cycle. Conflicting reports about the presence or significance of nitric oxide have been reported in the literature. The aim of this study was to investigate the role of nitric oxide and K ATP channels in human gallbladder muscle using isolated strips from human gallbladder. Methods: Full thickness strips were obtained from 56 human gallbladders and suspended under isometric tension in organ baths. The effect of nitric oxide donors and inhibitors on cholecystokinin octapeptide- and carbachol-induced contraction was examined. In separate experiments the effect of the K ATP channel activator, cromakalim, and the inhibitor, glibenclamide, were determined. Results: Cromakalim induced a significant relaxation of agonist-induced contraction in human gallbladder in vitro, an effect which was abolished by the K ATP channel inhibitor glibenclamide. No evidence of significant nitric oxide involvement in relaxation was observed. Conclusions: This study has demonstrated the presence of K ATP channels in human gallbladder for the first time. These are capable of causing significant relaxation in the presence of hormonal and muscarinic agonists and may represent a major pathway for gallbladder relaxation.</description><subject>Adenosine Triphosphate - physiology</subject><subject>Animals</subject><subject>Biological and medical sciences</subject><subject>Carbachol - pharmacology</subject><subject>Cromakalim - pharmacology</subject><subject>Digestive system</subject><subject>Gallbladder - physiology</subject><subject>Glyburide - pharmacology</subject><subject>Guinea Pigs</subject><subject>Humans</subject><subject>In Vitro Techniques</subject><subject>Investigative techniques, diagnostic techniques (general aspects)</subject><subject>Medical sciences</subject><subject>Muscle Relaxation - drug effects</subject><subject>Muscle Relaxation - physiology</subject><subject>Muscle, Smooth - physiology</subject><subject>Nitric Oxide - agonists</subject><subject>Nitric Oxide - antagonists & inhibitors</subject><subject>Nitric Oxide - physiology</subject><subject>Original Paper: Motility</subject><subject>Pathology. Cytology. Biochemistry. Spectrometry. Miscellaneous investigative techniques</subject><subject>Potassium Channels - chemistry</subject><subject>Potassium Channels - drug effects</subject><subject>Potassium Channels - physiology</subject><subject>Sincalide - pharmacology</subject><issn>0012-2823</issn><issn>1421-9867</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2002</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><recordid>eNpt0M9L5DAUB_AgKzqrHjwLSxQUPFSTvDaTHIdZHQcUxR9HKWn66tZN2zFpl53_3jozKoi5hJAP3_f4ErLL2QnniT5l_ZGgeLJGBjwWPNJKDn-QAWNcREIJ2CQ_Q3h-e-oYNsgmFwJ0LGFAHke2Lf8hvUVn_pu2bGraFPSiq0xNJ8a5zJk8R0-vumAd0mmgV5iXpsWcZnM6ur-J7rAO5SLjpmlNCGVX0fEfU9fowjZZL4wLuLO6t8jD-dn9-CK6vJ5Mx6PLyIKK2wg4B62sTkBrmSmZsBgShWB1EdtcDi1oLIDHSto8Y4pLZngiseAxMiUBYYscLXNnvnnpMLRpVQaLzpkamy6kQ8GZGILq4f4X-Nx0vu53S5Xuq2MLc7w01jcheCzSmS8r4-cpZ-lb3-lH3739tcrrsgrzT7kquAeHK2CCNa7wprZl-HSgRSK17t3e0v01_gn9B3gfc_Dt7-_pZAHSWV7AK0y7mUM</recordid><startdate>20020101</startdate><enddate>20020101</enddate><creator>Bird, N.C.</creator><creator>Ahmed, R.</creator><creator>Chess-Williams, R.</creator><creator>Johnson, A.G.</creator><general>Karger</general><general>S. Karger AG</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7QP</scope><scope>7X7</scope><scope>7XB</scope><scope>88E</scope><scope>8AO</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>BENPR</scope><scope>CCPQU</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>K9.</scope><scope>M0S</scope><scope>M1P</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>S0X</scope><scope>7X8</scope></search><sort><creationdate>20020101</creationdate><title>Active Relaxation of Human Gallbladder Muscle Is Mediated by ATP-Sensitive Potassium Channels</title><author>Bird, N.C. ; Ahmed, R. ; Chess-Williams, R. ; Johnson, A.G.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c384t-311398c953996b86504358e3c9f4cd67c39ef31486cdb08160a156ef14e0863e3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2002</creationdate><topic>Adenosine Triphosphate - physiology</topic><topic>Animals</topic><topic>Biological and medical sciences</topic><topic>Carbachol - pharmacology</topic><topic>Cromakalim - pharmacology</topic><topic>Digestive system</topic><topic>Gallbladder - physiology</topic><topic>Glyburide - pharmacology</topic><topic>Guinea Pigs</topic><topic>Humans</topic><topic>In Vitro Techniques</topic><topic>Investigative techniques, diagnostic techniques (general aspects)</topic><topic>Medical sciences</topic><topic>Muscle Relaxation - drug effects</topic><topic>Muscle Relaxation - physiology</topic><topic>Muscle, Smooth - physiology</topic><topic>Nitric Oxide - agonists</topic><topic>Nitric Oxide - antagonists & inhibitors</topic><topic>Nitric Oxide - physiology</topic><topic>Original Paper: Motility</topic><topic>Pathology. Cytology. Biochemistry. Spectrometry. Miscellaneous investigative techniques</topic><topic>Potassium Channels - chemistry</topic><topic>Potassium Channels - drug effects</topic><topic>Potassium Channels - physiology</topic><topic>Sincalide - pharmacology</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Bird, N.C.</creatorcontrib><creatorcontrib>Ahmed, R.</creatorcontrib><creatorcontrib>Chess-Williams, R.</creatorcontrib><creatorcontrib>Johnson, A.G.</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Calcium & Calcified Tissue Abstracts</collection><collection>Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Medical Database (Alumni Edition)</collection><collection>ProQuest Pharma Collection</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest Central UK/Ireland</collection><collection>ProQuest Central</collection><collection>ProQuest One Community College</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Medical Database</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central China</collection><collection>SIRS Editorial</collection><collection>MEDLINE - Academic</collection><jtitle>Digestion</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Bird, N.C.</au><au>Ahmed, R.</au><au>Chess-Williams, R.</au><au>Johnson, A.G.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Active Relaxation of Human Gallbladder Muscle Is Mediated by ATP-Sensitive Potassium Channels</atitle><jtitle>Digestion</jtitle><addtitle>Digestion</addtitle><date>2002-01-01</date><risdate>2002</risdate><volume>65</volume><issue>4</issue><spage>220</spage><epage>226</epage><pages>220-226</pages><issn>0012-2823</issn><eissn>1421-9867</eissn><coden>DIGEBW</coden><abstract>Background: Active and significant relaxation of the human gallbladder must be one of the facets of its motility during both the filling and emptying cycle. Conflicting reports about the presence or significance of nitric oxide have been reported in the literature. The aim of this study was to investigate the role of nitric oxide and K ATP channels in human gallbladder muscle using isolated strips from human gallbladder. Methods: Full thickness strips were obtained from 56 human gallbladders and suspended under isometric tension in organ baths. The effect of nitric oxide donors and inhibitors on cholecystokinin octapeptide- and carbachol-induced contraction was examined. In separate experiments the effect of the K ATP channel activator, cromakalim, and the inhibitor, glibenclamide, were determined. Results: Cromakalim induced a significant relaxation of agonist-induced contraction in human gallbladder in vitro, an effect which was abolished by the K ATP channel inhibitor glibenclamide. No evidence of significant nitric oxide involvement in relaxation was observed. Conclusions: This study has demonstrated the presence of K ATP channels in human gallbladder for the first time. These are capable of causing significant relaxation in the presence of hormonal and muscarinic agonists and may represent a major pathway for gallbladder relaxation.</abstract><cop>Basel, Switzerland</cop><pub>Karger</pub><pmid>12239463</pmid><doi>10.1159/000063815</doi><tpages>7</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0012-2823 |
| ispartof | Digestion, 2002-01, Vol.65 (4), p.220-226 |
| issn | 0012-2823 1421-9867 |
| language | eng |
| recordid | cdi_crossref_primary_10_1159_000063815 |
| source | MEDLINE; Karger Journals Complete |
| subjects | Adenosine Triphosphate - physiology Animals Biological and medical sciences Carbachol - pharmacology Cromakalim - pharmacology Digestive system Gallbladder - physiology Glyburide - pharmacology Guinea Pigs Humans In Vitro Techniques Investigative techniques, diagnostic techniques (general aspects) Medical sciences Muscle Relaxation - drug effects Muscle Relaxation - physiology Muscle, Smooth - physiology Nitric Oxide - agonists Nitric Oxide - antagonists & inhibitors Nitric Oxide - physiology Original Paper: Motility Pathology. Cytology. Biochemistry. Spectrometry. Miscellaneous investigative techniques Potassium Channels - chemistry Potassium Channels - drug effects Potassium Channels - physiology Sincalide - pharmacology |
| title | Active Relaxation of Human Gallbladder Muscle Is Mediated by ATP-Sensitive Potassium Channels |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2024-12-20T12%3A19%3A52IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Active%20Relaxation%20of%20Human%20Gallbladder%20Muscle%20Is%20Mediated%20by%20ATP-Sensitive%20Potassium%20Channels&rft.jtitle=Digestion&rft.au=Bird,%20N.C.&rft.date=2002-01-01&rft.volume=65&rft.issue=4&rft.spage=220&rft.epage=226&rft.pages=220-226&rft.issn=0012-2823&rft.eissn=1421-9867&rft.coden=DIGEBW&rft_id=info:doi/10.1159/000063815&rft_dat=%3Cproquest_cross%3E264256711%3C/proquest_cross%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=89142038&rft_id=info:pmid/12239463&rfr_iscdi=true |