Nitric Oxide Production by Pre-Implantation Embryos in Response to Embryotoxic Factors
In this report, we examined whether nitric oxide (NO) is involved in early embryo death. We have chosen various experimentally defined embryotoxic stimuli in mice and determined their ability to induce NO production by 2-cell stage embryos. The embryotoxic factors used were interferon-γ (IFN-γ), tum...
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| Veröffentlicht in: | Cellular physiology and biochemistry 2000-01, Vol.10 (3), p.169-176 |
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| container_title | Cellular physiology and biochemistry |
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| creator | Athanassakis, Irene Aifantis, Iannis Baritakis, Stavroula Farmakiotis, Vajia Koumantakis, Evgenios Vassiliadis, Simon |
| description | In this report, we examined whether nitric oxide (NO)
is involved in early embryo death. We have chosen various experimentally defined embryotoxic stimuli in mice and determined their ability to induce NO
production by 2-cell stage embryos. The embryotoxic factors used were interferon-γ (IFN-γ), tumor necrosis factor-α (TNF-α), lipopolysaccharide (LPS), 5-Azacytidine (5-AzaC) and the murine embryotoxic antibody DF4. We showed that in all cases the embryotoxic stimuli induced NO production by early stage embryos that correlated with the induction of the inducible and/or endothelial isoforms of NO synthase. This study was also extended to the human system where sera from women who aborted were tested for their ability to act embryotoxically by inducing NO in early mouse embryos and mature murine placenta. The results obtained confirmed the embryotoxic character of NO found in these particular sera leading to the hypothesis that NO plays a potential role in early embryo death. |
| doi_str_mv | 10.1159/000016347 |
| format | Article |
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is involved in early embryo death. We have chosen various experimentally defined embryotoxic stimuli in mice and determined their ability to induce NO
production by 2-cell stage embryos. The embryotoxic factors used were interferon-γ (IFN-γ), tumor necrosis factor-α (TNF-α), lipopolysaccharide (LPS), 5-Azacytidine (5-AzaC) and the murine embryotoxic antibody DF4. We showed that in all cases the embryotoxic stimuli induced NO production by early stage embryos that correlated with the induction of the inducible and/or endothelial isoforms of NO synthase. This study was also extended to the human system where sera from women who aborted were tested for their ability to act embryotoxically by inducing NO in early mouse embryos and mature murine placenta. The results obtained confirmed the embryotoxic character of NO found in these particular sera leading to the hypothesis that NO plays a potential role in early embryo death.</description><identifier>ISSN: 1015-8987</identifier><identifier>EISSN: 1421-9778</identifier><identifier>DOI: 10.1159/000016347</identifier><identifier>PMID: 10878447</identifier><language>eng</language><publisher>Basel, Switzerland</publisher><subject>Abortion, Spontaneous - blood ; Abortion, Spontaneous - etiology ; Animals ; Azacitidine - pharmacology ; Blastocyst - drug effects ; Blastocyst - metabolism ; Female ; Humans ; Interferon-gamma - pharmacology ; Lipopolysaccharides - pharmacology ; Mice ; Mice, Inbred BALB C ; Nitric Oxide - biosynthesis ; Original Paper ; Pregnancy ; Pregnancy Trimester, First ; Teratogens - analysis ; Teratogens - pharmacology ; Tumor Necrosis Factor-alpha - pharmacology</subject><ispartof>Cellular physiology and biochemistry, 2000-01, Vol.10 (3), p.169-176</ispartof><rights>2000 S. Karger AG, Basel</rights><rights>Copyright 2000 S. Karger AG, Basel.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c326t-ccfcbb75f3cdb4a1226072abb3a4401dd9b9e2f8510f01a52008cf4f55784c3a3</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>315,782,786,2431,27931,27932</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/10878447$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Athanassakis, Irene</creatorcontrib><creatorcontrib>Aifantis, Iannis</creatorcontrib><creatorcontrib>Baritakis, Stavroula</creatorcontrib><creatorcontrib>Farmakiotis, Vajia</creatorcontrib><creatorcontrib>Koumantakis, Evgenios</creatorcontrib><creatorcontrib>Vassiliadis, Simon</creatorcontrib><title>Nitric Oxide Production by Pre-Implantation Embryos in Response to Embryotoxic Factors</title><title>Cellular physiology and biochemistry</title><addtitle>Cell Physiol Biochem</addtitle><description>In this report, we examined whether nitric oxide (NO)
is involved in early embryo death. We have chosen various experimentally defined embryotoxic stimuli in mice and determined their ability to induce NO
production by 2-cell stage embryos. The embryotoxic factors used were interferon-γ (IFN-γ), tumor necrosis factor-α (TNF-α), lipopolysaccharide (LPS), 5-Azacytidine (5-AzaC) and the murine embryotoxic antibody DF4. We showed that in all cases the embryotoxic stimuli induced NO production by early stage embryos that correlated with the induction of the inducible and/or endothelial isoforms of NO synthase. This study was also extended to the human system where sera from women who aborted were tested for their ability to act embryotoxically by inducing NO in early mouse embryos and mature murine placenta. The results obtained confirmed the embryotoxic character of NO found in these particular sera leading to the hypothesis that NO plays a potential role in early embryo death.</description><subject>Abortion, Spontaneous - blood</subject><subject>Abortion, Spontaneous - etiology</subject><subject>Animals</subject><subject>Azacitidine - pharmacology</subject><subject>Blastocyst - drug effects</subject><subject>Blastocyst - metabolism</subject><subject>Female</subject><subject>Humans</subject><subject>Interferon-gamma - pharmacology</subject><subject>Lipopolysaccharides - pharmacology</subject><subject>Mice</subject><subject>Mice, Inbred BALB C</subject><subject>Nitric Oxide - biosynthesis</subject><subject>Original Paper</subject><subject>Pregnancy</subject><subject>Pregnancy Trimester, First</subject><subject>Teratogens - analysis</subject><subject>Teratogens - pharmacology</subject><subject>Tumor Necrosis Factor-alpha - pharmacology</subject><issn>1015-8987</issn><issn>1421-9778</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2000</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNptkEtLw0AUhQdRbK0uXAsS3LmIzrOTLLW0Wii2iLoN85RokwkzKTT_3rEp6sK7ua_vHi4HgHMEbxBi-S2MgcaE8gMwRBSjNOc8O4w1RCzN8owPwEkIHxFiPMfHYIBgxjNK-RC8PZWtL1Wy3JbaJCvv9Ea1pasT2cXOpPOqWYu6FbvZtJK-cyEp6-TZhMbVwSSt249bt406M6Fa58MpOLJiHczZPo_A62z6MnlMF8uH-eRukSqCx22qlFVScmaJ0pIKhPEYciykJIJSiLTOZW6wzRiCFiLBMISZstQyFt9XRJARuO51lXcheGOLxpeV8F2BYPHtTfHjTWQve7bZyMroP2RvRgQueuBT-Hfjf4H9-dW_28nqfgcUjbbkC5o5c-c</recordid><startdate>20000101</startdate><enddate>20000101</enddate><creator>Athanassakis, Irene</creator><creator>Aifantis, Iannis</creator><creator>Baritakis, Stavroula</creator><creator>Farmakiotis, Vajia</creator><creator>Koumantakis, Evgenios</creator><creator>Vassiliadis, Simon</creator><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope></search><sort><creationdate>20000101</creationdate><title>Nitric Oxide Production by Pre-Implantation Embryos in Response to Embryotoxic Factors</title><author>Athanassakis, Irene ; Aifantis, Iannis ; Baritakis, Stavroula ; Farmakiotis, Vajia ; Koumantakis, Evgenios ; Vassiliadis, Simon</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c326t-ccfcbb75f3cdb4a1226072abb3a4401dd9b9e2f8510f01a52008cf4f55784c3a3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2000</creationdate><topic>Abortion, Spontaneous - blood</topic><topic>Abortion, Spontaneous - etiology</topic><topic>Animals</topic><topic>Azacitidine - pharmacology</topic><topic>Blastocyst - drug effects</topic><topic>Blastocyst - metabolism</topic><topic>Female</topic><topic>Humans</topic><topic>Interferon-gamma - pharmacology</topic><topic>Lipopolysaccharides - pharmacology</topic><topic>Mice</topic><topic>Mice, Inbred BALB C</topic><topic>Nitric Oxide - biosynthesis</topic><topic>Original Paper</topic><topic>Pregnancy</topic><topic>Pregnancy Trimester, First</topic><topic>Teratogens - analysis</topic><topic>Teratogens - pharmacology</topic><topic>Tumor Necrosis Factor-alpha - pharmacology</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Athanassakis, Irene</creatorcontrib><creatorcontrib>Aifantis, Iannis</creatorcontrib><creatorcontrib>Baritakis, Stavroula</creatorcontrib><creatorcontrib>Farmakiotis, Vajia</creatorcontrib><creatorcontrib>Koumantakis, Evgenios</creatorcontrib><creatorcontrib>Vassiliadis, Simon</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><jtitle>Cellular physiology and biochemistry</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Athanassakis, Irene</au><au>Aifantis, Iannis</au><au>Baritakis, Stavroula</au><au>Farmakiotis, Vajia</au><au>Koumantakis, Evgenios</au><au>Vassiliadis, Simon</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Nitric Oxide Production by Pre-Implantation Embryos in Response to Embryotoxic Factors</atitle><jtitle>Cellular physiology and biochemistry</jtitle><addtitle>Cell Physiol Biochem</addtitle><date>2000-01-01</date><risdate>2000</risdate><volume>10</volume><issue>3</issue><spage>169</spage><epage>176</epage><pages>169-176</pages><issn>1015-8987</issn><eissn>1421-9778</eissn><abstract>In this report, we examined whether nitric oxide (NO)
is involved in early embryo death. We have chosen various experimentally defined embryotoxic stimuli in mice and determined their ability to induce NO
production by 2-cell stage embryos. The embryotoxic factors used were interferon-γ (IFN-γ), tumor necrosis factor-α (TNF-α), lipopolysaccharide (LPS), 5-Azacytidine (5-AzaC) and the murine embryotoxic antibody DF4. We showed that in all cases the embryotoxic stimuli induced NO production by early stage embryos that correlated with the induction of the inducible and/or endothelial isoforms of NO synthase. This study was also extended to the human system where sera from women who aborted were tested for their ability to act embryotoxically by inducing NO in early mouse embryos and mature murine placenta. The results obtained confirmed the embryotoxic character of NO found in these particular sera leading to the hypothesis that NO plays a potential role in early embryo death.</abstract><cop>Basel, Switzerland</cop><pmid>10878447</pmid><doi>10.1159/000016347</doi><tpages>8</tpages></addata></record> |
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| ispartof | Cellular physiology and biochemistry, 2000-01, Vol.10 (3), p.169-176 |
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| source | MEDLINE; Karger Journals |
| subjects | Abortion, Spontaneous - blood Abortion, Spontaneous - etiology Animals Azacitidine - pharmacology Blastocyst - drug effects Blastocyst - metabolism Female Humans Interferon-gamma - pharmacology Lipopolysaccharides - pharmacology Mice Mice, Inbred BALB C Nitric Oxide - biosynthesis Original Paper Pregnancy Pregnancy Trimester, First Teratogens - analysis Teratogens - pharmacology Tumor Necrosis Factor-alpha - pharmacology |
| title | Nitric Oxide Production by Pre-Implantation Embryos in Response to Embryotoxic Factors |
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