Decreased Expression Of apM1 in Omental and Subcutaneous Adipose Tissue of HumansWith Type 2 Diabetes
We have screened a subtracted cDNA library in order to identify differentially expressed genes in omental adipose tissue of human patients with Type 2 diabetes. One clone (#1738) showed a marked reduction in omental adipose tissue from patients with Type 2 diabetes. Sequencing and BLAST analysis rev...
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| Veröffentlicht in: | Journal of diabetes research 2000-01, Vol.1 (2), p.81-88 |
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| container_title | Journal of diabetes research |
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| creator | Statnick, Michael A. Beavers, Lisa S. Conner, Laura J. Corominola, Helena Johnson, Dwayne Hammond, Craig D. Rafaeloff-Phail, Ronit Seng, Thomas Suter, Todd M. Sluka, James P. Ravussin, Eric Gadski, Robert A. Caro, Jose F. |
| description | We have screened a subtracted cDNA library in
order to identify differentially expressed genes in
omental adipose tissue of human patients with
Type 2 diabetes. One clone (#1738) showed a marked
reduction in omental adipose tissue from patients
with Type 2 diabetes. Sequencing and BLAST analysis
revealed clone #1738 was the adipocyte‐specific
secreted protein gene apM1 (synonyms ACRP30,
AdipoQ, GBP28). Consistent with the murine orthologue,
apM1 mRNA was expressed in cultured
human adipocytes and not in preadipocytes.
Using RT‐PCR we confirmed that apM1 mRNA
levels were significantly reduced in omental adipose
tissue of obese patients with Type 2 diabetes compared
with lean and obese normoglycemic subjects.
Although less pronounced, apM1 mRNA levels
were reduced in subcutaneous adipose tissue of
Type 2 diabetic patients. Whereas the biological
function of apM1 is presently unknown, the tissue
specific expression, structural similarities to TNFα
and the dysregulated expression observed in obese
Type 2 diabetic patients suggest that this factor
may play a role in the pathogenesis of insulin resistance
and Type 2 diabetes. |
| doi_str_mv | 10.1155/EDR.2000.81 |
| format | Article |
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order to identify differentially expressed genes in
omental adipose tissue of human patients with
Type 2 diabetes. One clone (#1738) showed a marked
reduction in omental adipose tissue from patients
with Type 2 diabetes. Sequencing and BLAST analysis
revealed clone #1738 was the adipocyte‐specific
secreted protein gene apM1 (synonyms ACRP30,
AdipoQ, GBP28). Consistent with the murine orthologue,
apM1 mRNA was expressed in cultured
human adipocytes and not in preadipocytes.
Using RT‐PCR we confirmed that apM1 mRNA
levels were significantly reduced in omental adipose
tissue of obese patients with Type 2 diabetes compared
with lean and obese normoglycemic subjects.
Although less pronounced, apM1 mRNA levels
were reduced in subcutaneous adipose tissue of
Type 2 diabetic patients. Whereas the biological
function of apM1 is presently unknown, the tissue
specific expression, structural similarities to TNFα
and the dysregulated expression observed in obese
Type 2 diabetic patients suggest that this factor
may play a role in the pathogenesis of insulin resistance
and Type 2 diabetes.</description><identifier>ISSN: 2314-6745</identifier><identifier>EISSN: 2314-6753</identifier><identifier>DOI: 10.1155/EDR.2000.81</identifier><language>eng ; jpn</language><ispartof>Journal of diabetes research, 2000-01, Vol.1 (2), p.81-88</ispartof><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c1444-801be07b3273d8070df88724cade8a771e7af4c2b571ff3930dbbcc79803bede3</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,776,780,27903,27904</link.rule.ids></links><search><creatorcontrib>Statnick, Michael A.</creatorcontrib><creatorcontrib>Beavers, Lisa S.</creatorcontrib><creatorcontrib>Conner, Laura J.</creatorcontrib><creatorcontrib>Corominola, Helena</creatorcontrib><creatorcontrib>Johnson, Dwayne</creatorcontrib><creatorcontrib>Hammond, Craig D.</creatorcontrib><creatorcontrib>Rafaeloff-Phail, Ronit</creatorcontrib><creatorcontrib>Seng, Thomas</creatorcontrib><creatorcontrib>Suter, Todd M.</creatorcontrib><creatorcontrib>Sluka, James P.</creatorcontrib><creatorcontrib>Ravussin, Eric</creatorcontrib><creatorcontrib>Gadski, Robert A.</creatorcontrib><creatorcontrib>Caro, Jose F.</creatorcontrib><title>Decreased Expression Of apM1 in Omental and Subcutaneous Adipose Tissue of HumansWith Type 2 Diabetes</title><title>Journal of diabetes research</title><description>We have screened a subtracted cDNA library in
order to identify differentially expressed genes in
omental adipose tissue of human patients with
Type 2 diabetes. One clone (#1738) showed a marked
reduction in omental adipose tissue from patients
with Type 2 diabetes. Sequencing and BLAST analysis
revealed clone #1738 was the adipocyte‐specific
secreted protein gene apM1 (synonyms ACRP30,
AdipoQ, GBP28). Consistent with the murine orthologue,
apM1 mRNA was expressed in cultured
human adipocytes and not in preadipocytes.
Using RT‐PCR we confirmed that apM1 mRNA
levels were significantly reduced in omental adipose
tissue of obese patients with Type 2 diabetes compared
with lean and obese normoglycemic subjects.
Although less pronounced, apM1 mRNA levels
were reduced in subcutaneous adipose tissue of
Type 2 diabetic patients. Whereas the biological
function of apM1 is presently unknown, the tissue
specific expression, structural similarities to TNFα
and the dysregulated expression observed in obese
Type 2 diabetic patients suggest that this factor
may play a role in the pathogenesis of insulin resistance
and Type 2 diabetes.</description><issn>2314-6745</issn><issn>2314-6753</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2000</creationdate><recordtype>article</recordtype><recordid>eNo9kM1Kw0AURgdRsNSufIG7l9SZzMSZLEtbrVApaMVlmJ87ONImITcB-_amKK6-s_g4i8PYreBzIYrifr16neec87kRF2ySS6GyB13Iy39WxTWbEX2NH17K0hRmwnCFvkNLGGD93XZIlJoadhFs-yIgjXjEurcHsHWAt8H5obc1NgPBIqS2IYR9IhoQmgib4Whr-kj9J-xPLUIOq2Qd9kg37CraA-Hsb6fs_XG9X26y7e7pebnYZl4opTLDhUOuncy1DIZrHqIxOlfeBjRWa4HaRuVzV2gRoywlD855r0vDpcOAcsrufr2-a4g6jFXbpaPtTpXg1TlSNUaqzpEqI-QPKjlZxw</recordid><startdate>200001</startdate><enddate>200001</enddate><creator>Statnick, Michael A.</creator><creator>Beavers, Lisa S.</creator><creator>Conner, Laura J.</creator><creator>Corominola, Helena</creator><creator>Johnson, Dwayne</creator><creator>Hammond, Craig D.</creator><creator>Rafaeloff-Phail, Ronit</creator><creator>Seng, Thomas</creator><creator>Suter, Todd M.</creator><creator>Sluka, James P.</creator><creator>Ravussin, Eric</creator><creator>Gadski, Robert A.</creator><creator>Caro, Jose F.</creator><scope>AAYXX</scope><scope>CITATION</scope></search><sort><creationdate>200001</creationdate><title>Decreased Expression Of apM1 in Omental and Subcutaneous Adipose Tissue of HumansWith Type 2 Diabetes</title><author>Statnick, Michael A. ; Beavers, Lisa S. ; Conner, Laura J. ; Corominola, Helena ; Johnson, Dwayne ; Hammond, Craig D. ; Rafaeloff-Phail, Ronit ; Seng, Thomas ; Suter, Todd M. ; Sluka, James P. ; Ravussin, Eric ; Gadski, Robert A. ; Caro, Jose F.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c1444-801be07b3273d8070df88724cade8a771e7af4c2b571ff3930dbbcc79803bede3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng ; jpn</language><creationdate>2000</creationdate><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Statnick, Michael A.</creatorcontrib><creatorcontrib>Beavers, Lisa S.</creatorcontrib><creatorcontrib>Conner, Laura J.</creatorcontrib><creatorcontrib>Corominola, Helena</creatorcontrib><creatorcontrib>Johnson, Dwayne</creatorcontrib><creatorcontrib>Hammond, Craig D.</creatorcontrib><creatorcontrib>Rafaeloff-Phail, Ronit</creatorcontrib><creatorcontrib>Seng, Thomas</creatorcontrib><creatorcontrib>Suter, Todd M.</creatorcontrib><creatorcontrib>Sluka, James P.</creatorcontrib><creatorcontrib>Ravussin, Eric</creatorcontrib><creatorcontrib>Gadski, Robert A.</creatorcontrib><creatorcontrib>Caro, Jose F.</creatorcontrib><collection>CrossRef</collection><jtitle>Journal of diabetes research</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Statnick, Michael A.</au><au>Beavers, Lisa S.</au><au>Conner, Laura J.</au><au>Corominola, Helena</au><au>Johnson, Dwayne</au><au>Hammond, Craig D.</au><au>Rafaeloff-Phail, Ronit</au><au>Seng, Thomas</au><au>Suter, Todd M.</au><au>Sluka, James P.</au><au>Ravussin, Eric</au><au>Gadski, Robert A.</au><au>Caro, Jose F.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Decreased Expression Of apM1 in Omental and Subcutaneous Adipose Tissue of HumansWith Type 2 Diabetes</atitle><jtitle>Journal of diabetes research</jtitle><date>2000-01</date><risdate>2000</risdate><volume>1</volume><issue>2</issue><spage>81</spage><epage>88</epage><pages>81-88</pages><issn>2314-6745</issn><eissn>2314-6753</eissn><abstract>We have screened a subtracted cDNA library in
order to identify differentially expressed genes in
omental adipose tissue of human patients with
Type 2 diabetes. One clone (#1738) showed a marked
reduction in omental adipose tissue from patients
with Type 2 diabetes. Sequencing and BLAST analysis
revealed clone #1738 was the adipocyte‐specific
secreted protein gene apM1 (synonyms ACRP30,
AdipoQ, GBP28). Consistent with the murine orthologue,
apM1 mRNA was expressed in cultured
human adipocytes and not in preadipocytes.
Using RT‐PCR we confirmed that apM1 mRNA
levels were significantly reduced in omental adipose
tissue of obese patients with Type 2 diabetes compared
with lean and obese normoglycemic subjects.
Although less pronounced, apM1 mRNA levels
were reduced in subcutaneous adipose tissue of
Type 2 diabetic patients. Whereas the biological
function of apM1 is presently unknown, the tissue
specific expression, structural similarities to TNFα
and the dysregulated expression observed in obese
Type 2 diabetic patients suggest that this factor
may play a role in the pathogenesis of insulin resistance
and Type 2 diabetes.</abstract><doi>10.1155/EDR.2000.81</doi><tpages>8</tpages></addata></record> |
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| source | Wiley Online Library Open Access; Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals; Alma/SFX Local Collection |
| title | Decreased Expression Of apM1 in Omental and Subcutaneous Adipose Tissue of HumansWith Type 2 Diabetes |
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