A Comparative Validation Study of Fluconazole by HPLC and UPLC with Forced Degradation Study

A Comparative Validation Study of Fluconazole by HPLC and UPLC with Forced Degradation Study

The simplest stability indicating reversed phase Isocratic HPLC and UPLC methods has been developed and validated for the determination of fluconazole in bulk and solid pharmaceutical dosage form. A SunFire C18 (250 × 4.5 mm, 5 μm particle size) column has been used for HPLC and BEH C18 (100 × 2.1 m...

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Veröffentlicht in:Chromatography research international 2013-12, Vol.2013 (2013), p.1-5
Hauptverfasser: Jebaliya, Hetal, Patel, Madhavi, Jadeja, Yashwantsinh, Dabhi, Batuk, Shah, Anamik
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container_issue 2013
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container_title Chromatography research international
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creator Jebaliya, Hetal
Patel, Madhavi
Jadeja, Yashwantsinh
Dabhi, Batuk
Shah, Anamik
description The simplest stability indicating reversed phase Isocratic HPLC and UPLC methods has been developed and validated for the determination of fluconazole in bulk and solid pharmaceutical dosage form. A SunFire C18 (250 × 4.5 mm, 5 μm particle size) column has been used for HPLC and BEH C18 (100 × 2.1 mm, 1.7 μm particle size) column used for UPLC. The Mobile phase consisted of Methanol : Water (70 : 30) for HPLC and Methanol : Water (55 : 45 v/v) for UPLC. Isocratic flow was set at 1 mL/min and 0.30 mL/min, respectively, for HPLC and UPLC. For both HPLC and UPLC system detection has been performed at 211 nm with 30°C column oven temperature (good elution was obtained at 30°C) and injection volume, respectively, 2 μL and 20 μL for HPLC and UPLC.
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title A Comparative Validation Study of Fluconazole by HPLC and UPLC with Forced Degradation Study
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