GABA transporters regulate tonic and synaptic GABA A receptor-mediated currents in the suprachiasmatic nucleus neurons
GABA is a principal neurotransmitter in the hypothalamic suprachiasmatic nucleus (SCN) that contributes to intercellular communication between individual circadian oscillators within the SCN network and the stability and precision of the circadian rhythms. GABA transporters (GAT) regulate the extrac...
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| Veröffentlicht in: | Journal of neurophysiology 2017-12, Vol.118 (6), p.3092-3106 |
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| description | GABA is a principal neurotransmitter in the hypothalamic suprachiasmatic nucleus (SCN) that contributes to intercellular communication between individual circadian oscillators within the SCN network and the stability and precision of the circadian rhythms. GABA transporters (GAT) regulate the extracellular GABA concentration and modulate GABA
receptor (GABA
R)-mediated currents. GABA transport inhibitors were applied to study how GABA
R-mediated currents depend on the expression and function of GAT. Nipecotic acid inhibits GABA transport and induced an inward tonic current in concentration-dependent manner during whole cell patch-clamp recordings from SCN neurons. Application of either the selective GABA transporter 1 (GAT1) inhibitors NNC-711 or SKF-89976A, or the GABA transporter 3 (GAT3) inhibitor SNAP-5114, produced only small changes of the baseline current. Coapplication of GAT1 and GAT3 inhibitors induced a significant GABA
R-mediated tonic current that was blocked by gabazine. GAT inhibitors decreased the amplitude and decay time constant and increased the rise time of spontaneous GABA
R-mediated postsynaptic currents. However, inhibition of GAT did not alter the expression of either GAT1 or GAT3 in the hypothalamus. Thus GAT1 and GAT3 functionally complement each other to regulate the extracellular GABA concentration and GABA
R-mediated synaptic and tonic currents in the SCN. Coapplication of SKF-89976A and SNAP-5114 (50 µM each) significantly reduced the circadian period of
expression in the SCN by 1.4 h. Our studies demonstrate that GAT are important regulators of GABA
R-mediated currents and the circadian clock in the SCN.
In the suprachiasmatic nucleus (SCN), the GABA transporters GAT1 and GAT3 are expressed in astrocytes. Inhibition of these GABA transporters increased a tonic GABA current and reduced the circadian period of
expression in SCN neurons. GAT1 and GAT3 showed functional cooperativity: inhibition of one GAT increased the activity but not the expression of the other. Our data demonstrate that GABA transporters are important regulators of GABA
receptor-mediated currents and the circadian clock. |
| doi_str_mv | 10.1152/jn.00194.2017 |
| format | Article |
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receptor (GABA
R)-mediated currents. GABA transport inhibitors were applied to study how GABA
R-mediated currents depend on the expression and function of GAT. Nipecotic acid inhibits GABA transport and induced an inward tonic current in concentration-dependent manner during whole cell patch-clamp recordings from SCN neurons. Application of either the selective GABA transporter 1 (GAT1) inhibitors NNC-711 or SKF-89976A, or the GABA transporter 3 (GAT3) inhibitor SNAP-5114, produced only small changes of the baseline current. Coapplication of GAT1 and GAT3 inhibitors induced a significant GABA
R-mediated tonic current that was blocked by gabazine. GAT inhibitors decreased the amplitude and decay time constant and increased the rise time of spontaneous GABA
R-mediated postsynaptic currents. However, inhibition of GAT did not alter the expression of either GAT1 or GAT3 in the hypothalamus. Thus GAT1 and GAT3 functionally complement each other to regulate the extracellular GABA concentration and GABA
R-mediated synaptic and tonic currents in the SCN. Coapplication of SKF-89976A and SNAP-5114 (50 µM each) significantly reduced the circadian period of
expression in the SCN by 1.4 h. Our studies demonstrate that GAT are important regulators of GABA
R-mediated currents and the circadian clock in the SCN.
In the suprachiasmatic nucleus (SCN), the GABA transporters GAT1 and GAT3 are expressed in astrocytes. Inhibition of these GABA transporters increased a tonic GABA current and reduced the circadian period of
expression in SCN neurons. GAT1 and GAT3 showed functional cooperativity: inhibition of one GAT increased the activity but not the expression of the other. Our data demonstrate that GABA transporters are important regulators of GABA
receptor-mediated currents and the circadian clock.</description><identifier>ISSN: 0022-3077</identifier><identifier>EISSN: 1522-1598</identifier><identifier>DOI: 10.1152/jn.00194.2017</identifier><identifier>PMID: 28855287</identifier><language>eng</language><publisher>United States</publisher><subject>Animals ; Anisoles - pharmacology ; GABA Antagonists - pharmacology ; GABA Plasma Membrane Transport Proteins - metabolism ; GABA Uptake Inhibitors - pharmacology ; Male ; Neurons - drug effects ; Neurons - metabolism ; Neurons - physiology ; Nipecotic Acids - pharmacology ; Oximes - pharmacology ; Period Circadian Proteins - genetics ; Period Circadian Proteins - metabolism ; Rats ; Rats, Sprague-Dawley ; Receptors, GABA-A - metabolism ; Suprachiasmatic Nucleus - cytology ; Suprachiasmatic Nucleus - metabolism ; Suprachiasmatic Nucleus - physiology ; Synaptic Potentials</subject><ispartof>Journal of neurophysiology, 2017-12, Vol.118 (6), p.3092-3106</ispartof><rights>Copyright © 2017 the American Physiological Society.</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c190t-d67c0cefab9fa5cb755030944285639900d1bd0fd9be144ac18165d29bae79223</citedby><cites>FETCH-LOGICAL-c190t-d67c0cefab9fa5cb755030944285639900d1bd0fd9be144ac18165d29bae79223</cites><orcidid>0000-0002-3689-2878</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,3039,27924,27925</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/28855287$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Moldavan, Michael</creatorcontrib><creatorcontrib>Cravetchi, Olga</creatorcontrib><creatorcontrib>Allen, Charles N</creatorcontrib><title>GABA transporters regulate tonic and synaptic GABA A receptor-mediated currents in the suprachiasmatic nucleus neurons</title><title>Journal of neurophysiology</title><addtitle>J Neurophysiol</addtitle><description>GABA is a principal neurotransmitter in the hypothalamic suprachiasmatic nucleus (SCN) that contributes to intercellular communication between individual circadian oscillators within the SCN network and the stability and precision of the circadian rhythms. GABA transporters (GAT) regulate the extracellular GABA concentration and modulate GABA
receptor (GABA
R)-mediated currents. GABA transport inhibitors were applied to study how GABA
R-mediated currents depend on the expression and function of GAT. Nipecotic acid inhibits GABA transport and induced an inward tonic current in concentration-dependent manner during whole cell patch-clamp recordings from SCN neurons. Application of either the selective GABA transporter 1 (GAT1) inhibitors NNC-711 or SKF-89976A, or the GABA transporter 3 (GAT3) inhibitor SNAP-5114, produced only small changes of the baseline current. Coapplication of GAT1 and GAT3 inhibitors induced a significant GABA
R-mediated tonic current that was blocked by gabazine. GAT inhibitors decreased the amplitude and decay time constant and increased the rise time of spontaneous GABA
R-mediated postsynaptic currents. However, inhibition of GAT did not alter the expression of either GAT1 or GAT3 in the hypothalamus. Thus GAT1 and GAT3 functionally complement each other to regulate the extracellular GABA concentration and GABA
R-mediated synaptic and tonic currents in the SCN. Coapplication of SKF-89976A and SNAP-5114 (50 µM each) significantly reduced the circadian period of
expression in the SCN by 1.4 h. Our studies demonstrate that GAT are important regulators of GABA
R-mediated currents and the circadian clock in the SCN.
In the suprachiasmatic nucleus (SCN), the GABA transporters GAT1 and GAT3 are expressed in astrocytes. Inhibition of these GABA transporters increased a tonic GABA current and reduced the circadian period of
expression in SCN neurons. GAT1 and GAT3 showed functional cooperativity: inhibition of one GAT increased the activity but not the expression of the other. Our data demonstrate that GABA transporters are important regulators of GABA
receptor-mediated currents and the circadian clock.</description><subject>Animals</subject><subject>Anisoles - pharmacology</subject><subject>GABA Antagonists - pharmacology</subject><subject>GABA Plasma Membrane Transport Proteins - metabolism</subject><subject>GABA Uptake Inhibitors - pharmacology</subject><subject>Male</subject><subject>Neurons - drug effects</subject><subject>Neurons - metabolism</subject><subject>Neurons - physiology</subject><subject>Nipecotic Acids - pharmacology</subject><subject>Oximes - pharmacology</subject><subject>Period Circadian Proteins - genetics</subject><subject>Period Circadian Proteins - metabolism</subject><subject>Rats</subject><subject>Rats, Sprague-Dawley</subject><subject>Receptors, GABA-A - metabolism</subject><subject>Suprachiasmatic Nucleus - cytology</subject><subject>Suprachiasmatic Nucleus - metabolism</subject><subject>Suprachiasmatic Nucleus - physiology</subject><subject>Synaptic Potentials</subject><issn>0022-3077</issn><issn>1522-1598</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2017</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNo9kMFOwzAMhiMEYmNw5IryAh1O2izNsUwwkCZxgXOVJi7rtKVVkiLt7ck24GT712dL_gi5ZzBnTPDHrZsDMFXMOTB5QaYp4xkTqrwkU4DU5yDlhNyEsAUAKYBfkwkvSyF4Kafke1U9VTR67cLQ-4g-UI9f405HpLF3naHaWRoOTg8xDSe6SojBIfY-26PtEmqpGb1HFwPtHI0bpGEcvDabToe9Pi660exwDNTh6HsXbslVq3cB737rjHy-PH8sX7P1--ptWa0zwxTEzC6kAYOtblSrhWmkEJCDKgpeikWuFIBljYXWqgZZUWjDSrYQlqtGo1Sc5zOSne8a34fgsa0H3-21P9QM6qO_euvqk7_66C_xD2d-GJv02z_9Jyz_AcftbT0</recordid><startdate>20171201</startdate><enddate>20171201</enddate><creator>Moldavan, Michael</creator><creator>Cravetchi, Olga</creator><creator>Allen, Charles N</creator><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><orcidid>https://orcid.org/0000-0002-3689-2878</orcidid></search><sort><creationdate>20171201</creationdate><title>GABA transporters regulate tonic and synaptic GABA A receptor-mediated currents in the suprachiasmatic nucleus neurons</title><author>Moldavan, Michael ; Cravetchi, Olga ; Allen, Charles N</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c190t-d67c0cefab9fa5cb755030944285639900d1bd0fd9be144ac18165d29bae79223</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2017</creationdate><topic>Animals</topic><topic>Anisoles - pharmacology</topic><topic>GABA Antagonists - pharmacology</topic><topic>GABA Plasma Membrane Transport Proteins - metabolism</topic><topic>GABA Uptake Inhibitors - pharmacology</topic><topic>Male</topic><topic>Neurons - drug effects</topic><topic>Neurons - metabolism</topic><topic>Neurons - physiology</topic><topic>Nipecotic Acids - pharmacology</topic><topic>Oximes - pharmacology</topic><topic>Period Circadian Proteins - genetics</topic><topic>Period Circadian Proteins - metabolism</topic><topic>Rats</topic><topic>Rats, Sprague-Dawley</topic><topic>Receptors, GABA-A - metabolism</topic><topic>Suprachiasmatic Nucleus - cytology</topic><topic>Suprachiasmatic Nucleus - metabolism</topic><topic>Suprachiasmatic Nucleus - physiology</topic><topic>Synaptic Potentials</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Moldavan, Michael</creatorcontrib><creatorcontrib>Cravetchi, Olga</creatorcontrib><creatorcontrib>Allen, Charles N</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><jtitle>Journal of neurophysiology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Moldavan, Michael</au><au>Cravetchi, Olga</au><au>Allen, Charles N</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>GABA transporters regulate tonic and synaptic GABA A receptor-mediated currents in the suprachiasmatic nucleus neurons</atitle><jtitle>Journal of neurophysiology</jtitle><addtitle>J Neurophysiol</addtitle><date>2017-12-01</date><risdate>2017</risdate><volume>118</volume><issue>6</issue><spage>3092</spage><epage>3106</epage><pages>3092-3106</pages><issn>0022-3077</issn><eissn>1522-1598</eissn><abstract>GABA is a principal neurotransmitter in the hypothalamic suprachiasmatic nucleus (SCN) that contributes to intercellular communication between individual circadian oscillators within the SCN network and the stability and precision of the circadian rhythms. GABA transporters (GAT) regulate the extracellular GABA concentration and modulate GABA
receptor (GABA
R)-mediated currents. GABA transport inhibitors were applied to study how GABA
R-mediated currents depend on the expression and function of GAT. Nipecotic acid inhibits GABA transport and induced an inward tonic current in concentration-dependent manner during whole cell patch-clamp recordings from SCN neurons. Application of either the selective GABA transporter 1 (GAT1) inhibitors NNC-711 or SKF-89976A, or the GABA transporter 3 (GAT3) inhibitor SNAP-5114, produced only small changes of the baseline current. Coapplication of GAT1 and GAT3 inhibitors induced a significant GABA
R-mediated tonic current that was blocked by gabazine. GAT inhibitors decreased the amplitude and decay time constant and increased the rise time of spontaneous GABA
R-mediated postsynaptic currents. However, inhibition of GAT did not alter the expression of either GAT1 or GAT3 in the hypothalamus. Thus GAT1 and GAT3 functionally complement each other to regulate the extracellular GABA concentration and GABA
R-mediated synaptic and tonic currents in the SCN. Coapplication of SKF-89976A and SNAP-5114 (50 µM each) significantly reduced the circadian period of
expression in the SCN by 1.4 h. Our studies demonstrate that GAT are important regulators of GABA
R-mediated currents and the circadian clock in the SCN.
In the suprachiasmatic nucleus (SCN), the GABA transporters GAT1 and GAT3 are expressed in astrocytes. Inhibition of these GABA transporters increased a tonic GABA current and reduced the circadian period of
expression in SCN neurons. GAT1 and GAT3 showed functional cooperativity: inhibition of one GAT increased the activity but not the expression of the other. Our data demonstrate that GABA transporters are important regulators of GABA
receptor-mediated currents and the circadian clock.</abstract><cop>United States</cop><pmid>28855287</pmid><doi>10.1152/jn.00194.2017</doi><tpages>15</tpages><orcidid>https://orcid.org/0000-0002-3689-2878</orcidid><oa>free_for_read</oa></addata></record> |
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| source | MEDLINE; American Physiological Society Paid; EZB-FREE-00999 freely available EZB journals; Alma/SFX Local Collection |
| subjects | Animals Anisoles - pharmacology GABA Antagonists - pharmacology GABA Plasma Membrane Transport Proteins - metabolism GABA Uptake Inhibitors - pharmacology Male Neurons - drug effects Neurons - metabolism Neurons - physiology Nipecotic Acids - pharmacology Oximes - pharmacology Period Circadian Proteins - genetics Period Circadian Proteins - metabolism Rats Rats, Sprague-Dawley Receptors, GABA-A - metabolism Suprachiasmatic Nucleus - cytology Suprachiasmatic Nucleus - metabolism Suprachiasmatic Nucleus - physiology Synaptic Potentials |
| title | GABA transporters regulate tonic and synaptic GABA A receptor-mediated currents in the suprachiasmatic nucleus neurons |
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